pAAVLINKv1-pCALM1-Cre-3'-CUL7-FLAG

价格：元

规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg

相关技术服务：质粒构建    基因合成    质粒大提

联系方式：I47-825O-882O

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pAAVLINKv1-pCALM1-Cre-3'-CUL7-FLAG载体质粒基本信息

质粒名称:
pAAVLINKv1-pCALM1-Cre-3'-CUL7-FLAG
出品公司: Wekwikgene
目录编号: 0001857
产品用途: 3' AAVLINK plasmid for CUL7 expression
存储实验室: Zhonghua Lu Lab

载体骨架基本信息
载体名称: pAAV-MCS2
表达系统: 腺病毒
启动子:
pCALM1
拷贝数: 高拷贝
载体抗性: 氨苄霉素
生长温度: 37 ℃
宿主菌: DH5alpha

插入基因信息
插入基因名称: CUL7
物种来源: H.sapiens
密码子优化: 未优化

文献引用方法
参考文献部分:
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review

pAAVLINKv1-pCALM1-Cre-3'-CUL7-FLAG质粒图谱载体图谱和pAAVLINKv1-pCALM1-Cre-3'-CUL7-FLAG载体序列质粒序列多克隆位点信息

pAAVLINKv1-pCALM1-Cre-3-CUL7-FLAG载体图谱

pAAVLINKv1-pCALM1-Cre-3'-CUL7-FLAG质粒载体简介

pAAVLINKv1-pCALM1-Cre-3'-CUL7-FLAG质粒序列载体序列

LOCUS       pAAVLINKv1-pCALM1-Cre-3'-CUL7-FLAG               6412 bp DNA     circular SYN 24-NOV-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6412)
  AUTHORS   Stavrini Papadaki
  TITLE     Direct Submission
  JOURNAL   Exported Jun 10, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..6412
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
                     /note="color: #ffe4c4"
     promoter        145..265
                     /label=pCALM1
     CDS             277..1308
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
                     LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
                     PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
                     LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
                     RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
                     RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
                     DGD"
     intron          1309..1349
                     /label=Splicing Donor
     protein_bind    1356..1389
                     /label=lox2272-JTZ17
                     /bound_moiety="Cre recombinase"
                     /note="Right element (RE) mutant of loxP (Araki et al., 
                     2010). Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     intron          1390..1481
                     /label=Splicing Acceptor
                     /note="Splicing acceptor"
     CDS             1482..3113
                     /codon_start=1
                     /gene="CUL7"
                     /gene_synonym="3M1; CUL-7; dJ20C7.5; KIAA0076"
                     /product="cullin-7 isoform 2"
                     /label=CUL7
                     /note="isoform 2 is encoded by transcript variant 2"
                     /db_xref="GI:41872646"
                     /db_xref="CCDS:CCDS4881.1"
                     /db_xref="GeneID:9820"
                     /db_xref="HGNC:HGNC:21024"
                     /db_xref="MIM:609577"
                     /protein_id="NP_055595.2"
                     /translation="VNNFLTSSWRDDDFVPRYCEHFNILQNSSSELFGPRAAFLLALQN
                     GCAGALLKLPFLKAAHVSEQFARHIDQQIQGSRIGGAQEMERLAQLQQCLQAVLIFSGL
                     EIATTFEHYYQHYMADRLLGVVSSWLEGAVLEQIGPCFPNRLPQQMLQSLSTSKELQRQ
                     FHVYQLQQLDQELLKLEDTEKKIQVGLGASGKEHKSEKEEEAGAAAVVDVAEGEEEEEE
                     NEDLYYEGAMPEVSVLVLSRHSWPVASICHTLNPRTCLPSYLRGTLNRYSNFYNKSQSH
                     PALERGSQRRLQWTWLGWAELQFGNQTLHVSTVQMWLLLYLNDLKAVSVESLPAFSGLS
                     ADMLNQAIGPLTSSRGPLDLHEQKDIPGGVLKIRDGSKEPRSRWDIVRLIPPQTYLQAE
                     GEDGQNLEKRRNLLNCLIVRILKAHGDEGLHIDQLVCLVLEAWQKGPCPPRGLVSSLGK
                     GSACSSTDVLSCILHLLGKGTLRRHDDRPQVLSYAVPVTVMEPHTESLNPGSSGPNPPL
                     TFHTLQIRSRGVPYASCTATQSFSTFR"
     misc_feature    2131..2136
                     /label=SDS
     misc_feature    2140..2145
                     /label=SDS
     misc_feature    2263..2268
                     /label=SDS
     misc_feature    2582..2587
                     /label=SDS
     misc_feature    2900..2905
                     /label=SDS
     misc_feature    3076..3081
                     /label=SDS
     CDS             3120..3185
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     terminator      3201..3632
                     /label=W3SL
                     /note="Short WPRE and SV40 polyA"
     misc_feature    3345..3350
                     /label=SDS
     repeat_region   3675..3815
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     misc_feature    3675..3804
                     /label=AAV2 ITR
     repeat_region   3676..3816
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     rep_origin      3890..4345
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        4627..4731
                     /gene="bla"
                     /label=AmpR promoter
     CDS             4732..5592
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      5763..6351
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtcgctct gcgcgaggcg gctccgccgc ggcggaggga
      181 tacggcgcac catatatata tcgcggggcg cagactcgcg ctccggcagt ggtgctggga
      241 gtgtcgtgga cgccgtgccg ttactcgtac gccaccatgt ccaatttact gaccgtacac
      301 caaaatttgc ctgcattacc ggtcgatgca acgagtgatg aggttcgcaa gaacctgatg
      361 gacatgttca gggatcgcca ggcgttttct gagcatacct ggaaaatgct tctgtccgtt
      421 tgccggtcgt gggcggcatg gtgcaagttg aataaccgga aatggtttcc cgcagaacct
      481 gaagatgttc gcgattatct tctatatctt caggcgcgcg gtctggcagt aaaaaccatc
      541 cagcaacatt tgggccagct aaacatgctt catcgtcggt ccgggctgcc acgaccaagt
      601 gacagcaatg ctgtttcact ggttatgcgg cggatccgaa aagaaaacgt tgatgccggt
      661 gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt tcgaccaggt tcgttcactc
      721 atggaaaata gcgatcgctg ccaggatata cgtaatctgg catttctggg gattgcttat
      781 aacaccctgt tacgtatagc cgaaattgcc aggatcaggg ttaaagatat ctcacgtact
      841 gacggtggga gaatgttaat ccatattggc agaacgaaaa cgctggttag caccgcaggt
      901 gtagagaagg cacttagcct gggggtaact aaactggtcg agcgatggat ttccgtctct
      961 ggtgtagctg atgatccgaa taactacctg ttttgccggg tcagaaaaaa tggtgttgcc
     1021 gcgccatctg ccaccagcca gctatcaact cgcgccctgg aagggatttt tgaagcaact
     1081 catcgattga tttacggcgc taaggatgac tctggtcaga gatacctggc ctggtctgga
     1141 cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg ctggagtttc aataccggag
     1201 atcatgcaag ctggtggctg gaccaatgta aatattgtaa tgaactatat ccgtaacctg
     1261 gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg gcgattaggt aagtatcaag
     1321 gttacaagac aggtttaagg agaccaataa ctagtataac ttcgtatagg atactttata
     1381 gcaatttatg aaactgggct tgtcgagaca gagaagactc ttgcgtttct gataggcacc
     1441 tattggtctt actgacatcc actttgcctt tctctccaca ggtgaacaat tttctgacct
     1501 catcctggcg ggatgatgac tttgtgccac gctactgtga gcactttaat attctgcaga
     1561 actcaagctc tgaactgttt gggcctcggg cagccttctt gctggcgctg caaaatggct
     1621 gtgcgggagc cttgctgaag ctcccttttc tcaaagctgc ccacgtgagt gagcagttcg
     1681 cccggcacat tgaccagcag atccagggca gccggatcgg tggagcccag gaaatggaga
     1741 ggctggcaca gctgcagcaa tgcctgcaag ctgtcctgat tttctccggc ttggagatag
     1801 ccaccacttt tgagcattat taccagcact acatggcgga ccgtctcctg ggcgtggtct
     1861 cgagctggct ggagggggcc gtgctggagc agatcggtcc ctgcttcccc aaccgcctcc
     1921 cccagcagat gttgcagagc ctgagcacct ctaaggagct gcagcgccag ttccacgtct
     1981 accagctcca gcagctggat caggaactcc tgaagctgga ggatacagag aagaaaatac
     2041 aggtgggcct tggggccagt ggcaaggagc acaagagcga gaaggaagag gaagctgggg
     2101 cagcagcagt ggtggatgtg gcggagggag aggaggaaga ggaggagaat gaggacctct
     2161 actatgaagg ggcaatgcca gaagtgtctg tgcttgtcct gtcccgacac tcctggcctg
     2221 ttgcctcaat ctgccacaca ctgaacccca gaacctgcct gccctcctac ctgaggggca
     2281 ctttgaacag atactccaac ttctacaaca agagtcagag ccaccctgcc cttgagcgag
     2341 gctcacagag gcgactgcag tggacgtggc tgggctgggc tgagctgcag tttgggaacc
     2401 agaccctgca tgtgtccacc gtgcagatgt ggctactgct gtatctcaac gacctgaagg
     2461 cggtctctgt ggagagtctg ccggcgttct cagggctctc cgcagacatg ctcaatcagg
     2521 cgattgggcc cctcacctct tcaagaggcc ccctggacct tcacgagcaa aaggatatac
     2581 caggaggggt cctcaagatt cgagatggca gcaaggaacc caggtcgaga tgggacattg
     2641 tgcggctcat cccacctcag acgtacctgc aagctgaggg tgaagacggc cagaacttgg
     2701 agaagagacg gaatcttctg aactgcctca tcgtccgaat cctcaaggcc catggagatg
     2761 aggggctgca cattgaccag cttgtctgtc tggtgctgga ggcttggcag aagggcccgt
     2821 gtcctcccag gggtttggtc agcagccttg gtaaggggtc tgcatgcagc agcactgacg
     2881 tcctctcctg catcctacac ctcctgggca agggcacgct gagacgccat gacgaccggc
     2941 cccaggtgct gtcctatgca gtccctgtga ctgtcatgga gcctcacact gagtccctga
     3001 acccaggctc ctcaggcccc aacccacccc tcaccttcca taccctacag attcgctccc
     3061 ggggtgtgcc ctatgcctcc tgcactgcca cccagagctt ctctaccttc cggtgtacag
     3121 actacaaaga ccatgacggt gattataaag atcatgacat cgactacaag gatgacgatg
     3181 acaagtaaga attctgatga ctcgagataa tcaacctctg gattacaaaa tttgtgaaag
     3241 attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat
     3301 gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc
     3361 ctggttagtt cttgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg
     3421 ggctcggctg ttgggcactg acaattccgt ggtgtttatt tgtgaaattt gtgatgctat
     3481 tgctttattt gtaaccatct agctttattt gtgaaatttg tgatgctatt gctttatttg
     3541 taaccattat aagctgcaat aaacaagtta acaacaacaa ttgcattcat tttatgtttc
     3601 aggttcaggg ggagatgtgg gaggtttttt aaagatctgg agaccggtgt ggcacgtgcg
     3661 gaccgagcgg ccgcaggaac ccctagtgat ggagttggcc actccctctc tgcgcgctcg
     3721 ctcgctcact gaggccgggc gaccaaaggt cgcccgacgc ccgggctttg cccgggcggc
     3781 ctcagtgagc gagcgagcgc gcagctgcct gcaggggcgc ctgatgcggt attttctcct
     3841 tacgcatctg tgcggtattt cacaccgcat acgtcaaagc aaccatagta cgcgccctgt
     3901 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
     3961 agcgccttag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
     4021 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg
     4081 cacctcgacc ccaaaaaact tgatttgggt gatggttcac gtagtgggcc atcgccctga
     4141 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc
     4201 caaactggaa caacactcaa ctctatctcg ggctattctt ttgatttata agggattttg
     4261 ccgatttcgg tctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt
     4321 aacaaaatat taacgtttac aattttatgg tgcactctca gtacaatctg ctctgatgcc
     4381 gcatagttaa gccagccccg acacccgcca acacccgctg acgcgccctg acgggcttgt
     4441 ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag
     4501 aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt
     4561 ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga
     4621 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
     4681 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt
     4741 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct
     4801 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt
     4861 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt
     4921 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac
     4981 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac
     5041 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct
     5101 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg
     5161 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg
     5221 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca
     5281 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa
     5341 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt
     5401 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc
     5461 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg
     5521 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
     5581 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt
     5641 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
     5701 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     5761 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     5821 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     5881 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac
     5941 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     6001 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     6061 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     6121 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
     6181 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     6241 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     6301 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     6361 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gt
//

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pAAVLINKv1-pCALM1-Cre-3'-CUL7-FLAG质粒载体应用举例

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