| 质粒名称: | pAAV-CAG-NES-FRCaM |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0000182 |
| 产品用途: | Expresses the variant of FRCaMP red calcium indicator based on calmodulin from S.pombe fungus with deleted M13-peptide and blocked sensitivity to calcium ions in the cytosol of mammalian cells |
| 存储实验室: | Laboratory of Molecular Design |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV |
| 表达系统: | 腺病毒，哺乳动物表达 |
| 启动子: | CAG |
| 拷贝数: | 低拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | Mach1 |
|
插入基因信息 |
|
| 插入基因名称: | FRCaM |
| 物种来源: | Synthetic |
| 密码子优化: | 细菌表达优化 |
|
文献引用方法 |
|
| 参考文献部分: |
FRCaMP, a Red Fluorescent Genetically Encoded Calcium Indicator Based on Calmodulin from Schizosaccharomyces Pombe Fungus
Subach O.M., Barykina N.V., Chefanova E.S., Vlaskina A.V., Sotskov V.P., Ivashkina O.I., Anokhin K.V., Subach F.V.
Int. J. Mol. Sci. 2021, Vol. 22, Page 111 22, 111 (2020)
|
买家导航
pAAV-CAG-NES-FRCaM载体质粒基本信息
pAAV-CAG-NES-FRCaM质粒图谱载体图谱和pAAV-CAG-NES-FRCaM载体序列质粒序列多克隆位点信息
pAAV-CAG-NES-FRCaM质粒载体简介
pAAV-CAG-NES-FRCaM质粒序列载体序列
LOCUS pAAV-CAG-NES-FRCaM 6001 bp DNA circular SYN 22-APR-2023
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6001)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Apr 29, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6001
/mol_type="other DNA"
/organism="synthetic DNA construct"
rep_origin complement(182..770)
/direction=LEFT
/label=ori
/note="high-copy-number colE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(941..1801)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(1802..1906)
/gene="bla"
/label=AmpR promoter
rep_origin complement(1932..2387)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 2942..3219
/label=chicken beta-actin promoter
CDS 3652..3678
/codon_start=1
/product="nuclear export signal from the HIV Rev protein
(Fischer et al., 1995)"
/label=NES
/translation="LPPLERLTL"
CDS 3688..4890
/codon_start=1
/label=FRCaM
/translation="VPRVSEWMYPEDGALKSEIRKGLRLKDGGHYAAEVKTTYKAKKPV
QLPGAYIVDIELDILSHNEDYTIVEQCERAVGRHPTGGTDALYKGGTGSGGSLVSKGEE
DNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEAFQTARLKVTKGGPLPFAWDIL
SPQFMYGSKAYIKHPADIPDYFKLSFPEGFRWERVMSFEDGGIIHVNQDSSLQDGVFIY
KVKLLGTNFPPDGPVMQKETMGWEASYEMTTRNLTDEQIAEFREAFSLLDRDQDGNITS
NELGVVMRSLGLSPTAAELQDMINEVDADGNGTIDFTEFLTMMARKMKDTDDEEEVREA
FKVFDKDGSGYITVEELTHVLTSLGERLSREEVADVIREADSDGDGVINYEEFSRVISS
K"
primer_bind complement(4272..4294)
/label=WKG181-R
misc_feature 4903..5491
/label=WPRE
/note="woodchuck posttranscriptional regulatory element"
CDS complement(5374..5385)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
polyA_signal 5534..5655
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
ORIGIN
1 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
61 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
121 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
181 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
241 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
301 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
361 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
421 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
481 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
541 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
601 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
661 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
721 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
781 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
841 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
901 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
961 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
1021 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
1081 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
1141 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
1201 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
1261 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
1321 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
1381 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
1441 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
1501 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
1561 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
1621 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
1681 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
1741 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
1801 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
1861 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
1921 aagtgccacc taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta
1981 aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga
2041 atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa
2101 cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga
2161 accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc
2221 taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga
2281 agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg
2341 cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtccc attcgccatt
2401 caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct
2461 gcgcgctcgc tcgctcactg aggccgcccg ggcaaagccc gggcgtcggg cgacctttgg
2521 tcgcccggcc tcagtgagcg agcgagcgcg cagagaggga gtggccaact ccatcactag
2581 gggttccttg tagttaatga ttaacccgcc atgctactta tctacgtagc catgctctag
2641 gaagatcgta ggttaattaa ggcgcgcccc taggggagat ggccagccag cccattgacg
2701 tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg
2761 gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt
2821 acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg
2881 accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg
2941 gtcgaggtga gccccacgtt ctgcttcact ctccccatct cccccccctc cccaccccca
3001 attttgtatt tatttatttt ttaattattt tgtgcagcga tgggggcggg gggggggggg
3061 gggcgcgcgc caggcggggc ggggcggggc gaggggcggg gcggggcgag gcggagaggt
3121 gcggcggcag ccaatcagag cggcgcgctc cgaaagtttc cttttatggc gaggcggcgg
3181 cggcggcggc cctataaaaa gcgaagcgcg cggcgggcgg gagtcgctgc gacgctgcct
3241 tcgccccgtg ccccgctccg ccgccgcctc gcgccgcccg ccccggctct gactgaccgc
3301 gttactccca caggtgagcg ggcgggacgg cccttctcct ccgggctgta attagcgctt
3361 ggtttaatga cggcttgttt cttttctgtg gctgcgtgaa agccttgagg ggctccggga
3421 gggccctttg tgcgggggga gcggctcggg gctgtccgcg gggggacggc tgccttcggg
3481 ggggacgggg cagggcgggg ttcggcttct ggcgtgtgac cggcggctct agagcctctg
3541 ctaaccatgt tcatgccttc ttctttttcc tacagctcct gggcaacgtg ctggttattg
3601 tgctgtctca tcattttggc aaagaattgg ggatccgaca ccatgcttca acttcctcct
3661 cttgaacgtc ttactctttc gagatctgtg ccgcgggttt ccgagtggat gtaccccgag
3721 gacggcgccc tgaagagcga gatcaggaag gggctgaggc tgaaggacgg cggccactac
3781 gccgccgagg tcaagaccac ctacaaggcc aagaagcccg tgcagctgcc cggcgcctac
3841 atcgtcgaca tcgagttgga catcttgtcc cacaacgagg actacaccat cgtggaacag
3901 tgcgaacgcg ccgtgggccg ccaccccacc ggtggcacgg acgcgctgta caagggaggt
3961 acaggctccg gcgggagtct ggtaagcaag ggcgaggagg ataacatggc catcatcaag
4021 gagttcatgc gcttcaaggt gcacatggag ggctccgtga acggccacga gttcgagatc
4081 gagggcgagg gcgagggccg cccctacgag gccttccaga ccgctaggct gaaggtgacc
4141 aagggtggcc ccctgccctt cgcctgggac atcctgtccc ctcagttcat gtacggctcc
4201 aaggcctaca ttaagcaccc agccgacatc cccgactact tcaagctgtc cttccccgag
4261 ggcttcaggt gggagcgcgt gatgagcttc gaggacggcg gcattattca cgttaatcag
4321 gactcctccc tgcaggacgg cgtattcatc tacaaggtga agctgctcgg caccaacttc
4381 ccccccgacg gccccgtaat gcagaaagag accatgggct gggaggcttc ctacgagatg
4441 actacccgta accttacaga tgagcagatt gcggagttcc gtgaggcctt ttcgctgctt
4501 gatcgtgatc aggatggaaa tatcacgtcc aatgaattgg gtgtggttat gaggtcgtta
4561 ggtctatcgc ctactgccgc cgaattacaa gatatgatta atgaggtcga tgccgatggt
4621 aatggcacaa ttgattttac cgaatttttg acaatgatgg cccggaaaat gaaggatacc
4681 gacgacgaag aggaagttcg cgaagccttt aaagtcttcg ataaagatgg aagtggatac
4741 attacagtcg aggagctgac tcatgttctt acaagtctcg gtgaacgttt gtctcgagaa
4801 gaagtagccg atgtgatacg tgaagccgac tccgatggcg atggtgtaat caactacgaa
4861 gaattctctc gtgtcatttc ttccaagtaa aagcttatcg ataatcaacc tctggattac
4921 aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga
4981 tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc
5041 tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa
5101 cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc
5161 acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc
5221 atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc
5281 gtggtgttgt cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg
5341 attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct
5401 tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg
5461 agtcggatct ccctttgggc cgcctccccg catcgatacc gtcgacccgg gcggccgctt
5521 cgagcagaca tgataagata cattgatgag tttggacaaa ccacaactag aatgcagtga
5581 aaaaaatgct ttatttgtga aatttgtgat gctattgctt tatttgtaac cattataagc
5641 tgcaataaac aagttaacaa caacaattgc attcatttta tgtttcaggt tcagggggag
5701 atgtgggagg ttttttaaag caagtaaaac ctctacaaat gtggtaaaat cgataaggat
5761 cttcctagag catggctacg tagataagta gcatggcggg ttaatcatta actacaagga
5821 acccctagtg atggagttgg ccactccctc tctgcgcgct cgctcgctca ctgaggccgg
5881 gcgaccaaag gtcgcccgac gcccgggctt tgcccgggcg gcctcagtga gcgagcgagc
5941 gcgcagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc
6001 t
//
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