pTCK303-bk1-5HA-bk2

LOCUS       pTCK303-bk1-5HA-bk2         15510 bp DNA     circular SYN 25-MAR-2026
DEFINITION  pTCK303.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15510)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 22, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..15510
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(23..368)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     745..761
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        777..2769
                     /label=Ubi promoter
                     /note="maize polyubiquitin gene promoter"
     misc_feature    2686..2703
                     /label=303F
     CDS             2818..2898
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
     CDS             2845..2925
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
     CDS             2872..2952
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
     misc_feature    2959..2979
                     /label=同源臂
     misc_feature    2992..3012
                     /label=同源臂
     CDS             3013..3022
                     /codon_start=1
                     /label=Feature 22
                     /translation="EFP"
     terminator      3023..3275
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    3072..3091
                     /label=NOSR
     primer_bind     complement(3292..3308)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    3316..3332
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3340..3370)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3385..3406
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        3597..4273
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     CDS             4340..5365
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     polyA_signal    5406..5580
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    5658..5682
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             6107..6901
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      8187..8775
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    8961..9101
                     /label=bom
                     /note="basis of mobility region from pBR322"
     CDS             complement(11207..11836)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    13137..13161
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     terminator      13183..13435
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(13470..13487)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(join(13494..15311,15502..15510,1..6))
                     /codon_start=1
                     /product="beta-glucuronidase"
                     /label=GUS
                     /note="This version of the gusA gene has a 5' extension 
                     with a catalase intron to ensure expression in plants but 
                     not bacteria."
                     /translation="MVDLRNRRLVRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWES
                     ALQESRAIAVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGK
                     VWVNNQEVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGK
                     KKQSYFHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSV
                     ELRDADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRS
                     VAVKGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYP
                     YAEEMLDWADEHGIVVIDETAAVGFQLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQA
                     IKELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVDT
                     LAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGNKK
                     GIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     intron          15312..15501
                     /label=cat1 intron
                     /note="castor bean catalase intron, modified"
ORIGIN
        1 taccatggtc aagagtcccc cgtgttctct ccaaatgaaa tgaacttcct tatatagagg
       61 aagggtcttg cgaaggatag tgggattgtg cgtcatccct tacgtcagtg gagatatcac
      121 atcaatccac ttgctttgaa gacgtggttg gaacgtcttc tttttccacg atgctcctcg
      181 tgggtggggg tccatctttg ggaccactgt cggcagaggc atcttcaacg atggcctttc
      241 ctttatcgca atgatggcat ttgtaggagc caccttcctt ttccactatc ttcacaataa
      301 agtgacagat agctgggcaa tggaatccga ggaggtttcc ggatattacc ctttgttgaa
      361 aagtctcaat tgccctttgg tcttctgaga ctgtatcttt gatatttttg gagtagacaa
      421 gtgtgtcgtg ctccaccatg ttgacgaaga ttttcttctt gtcattgagt cgtaagagac
      481 tctgtatgaa ctgttcgcca gtctttacgg cgagttctgt taggtcctct atttgaatct
      541 ttgactccat gaagctaaac tgaaggcggg aaacgacaat ctgatccaag ctcaagctgc
      601 tctagcattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt
      661 cgctattacg ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc
      721 cagggttttc ccagtcacga cgttgtaaaa cgacggccag tgccaagctt gcatgcctgc
      781 agtgcagcgt gacccggtcg tgcccctctc tagagataat gagcattgca tgtctaagtt
      841 ataaaaaatt accacatatt ttttttgtca cacttgtttg aagtgcagtt tatctatctt
      901 tatacatata tttaaacttt actctacgaa taatataatc tatagtacta caataatatc
      961 agtgttttag agaatcatat aaatgaacag ttagacatgg tctaaaggac aattgagtat
     1021 tttgacaaca ggactctaca gttttatctt tttagtgtgc atgtgttctc cttttttttt
     1081 gcaaatagct tcacctatat aatacttcat ccattttatt agtacatcca tttagggttt
     1141 agggttaatg gtttttatag actaattttt ttagtacatc tattttattc tattttagcc
     1201 tctaaattaa gaaaactaaa actctatttt agttttttta tttaataatt tagatataaa
     1261 atagaataaa ataaagtgac taaaaattaa acaaataccc tttaagaaat taaaaaaact
     1321 aaggaaacat ttttcttgtt tcgagtagat aatgccagcc tgttaaacgc cgtcgacgag
     1381 tctaacggac accaaccagc gaaccagcag cgtcgcgtcg ggccaagcga agcagacggc
     1441 acggcatctc tgtcgctgcc tctggacccc tctcgagagt tccgctccac cgttggactt
     1501 gctccgctgt cggcatccag aaattgcgtg gcggagcggc agacgtgagc cggcacggca
     1561 ggcggcctcc tcctcctctc acggcaccgg cagctacggg ggattccttt cccaccgctc
     1621 cttcgctttc ccttcctcgc ccgccgtaat aaatagacac cccctccaca ccctctttcc
     1681 ccaacctcgt gttgttcgga gcgcacacac acacaaccag atctccccca aatccacccg
     1741 tcggcacctc cgcttcaagg tacgccgctc gtcctccccc cccccccctc tctaccttct
     1801 ctagatcggc gttccggtcc atggttaggg cccggtagtt ctacttctgt tcatgtttgt
     1861 gttagatccg tgtttgtgtt agatccgtgc tgctagcgtt cgtacacgga tgcgacctgt
     1921 acgtcagaca cgttctgatt gctaacttgc cagtgtttct ctttggggaa tcctgggatg
     1981 gctctagccg ttccgcagac gggatcgatt tcatgatttt ttttgtttcg ttgcataggg
     2041 tttggtttgc ccttttcctt tatttcaata tatgccgtgc acttgtttgt cgggtcatct
     2101 tttcatgctt ttttttgtct tggttgtgat gatgtggtct ggttgggcgg tcgttctaga
     2161 tcggagtaga attctgtttc aaactacctg gtggatttat taattttgga tctgtatgtg
     2221 tgtgccatac atattcatag ttacgaattg aagatgatgg atggaaatat cgatctagga
     2281 taggtataca tgttgatgcg ggttttactg atgcatatac agagatgctt tttgttcgct
     2341 tggttgtgat gatgtggtgt ggttgggcgg tcgttcattc gttctagatc ggagtagaat
     2401 actgtttcaa actacctggt gtatttatta attttggaac tgtatgtgtg tgtcatacat
     2461 cttcatagtt acgagtttaa gatggatgga aatatcgatc taggataggt atacatgttg
     2521 atgtgggttt tactgatgca tatacatgat ggcatatgca gcatctattc atatgctcta
     2581 accttgagta cctatctatt ataataaaca agtatgtttt ataattattt tgatcttgat
     2641 atacttggat gatggcatat gcagcagcta tatgtggatt tttttagccc tgccttcata
     2701 cgctatttat ttgcttggta ctgtttcttt tgtcgatgct caccctgttg tttggtgtta
     2761 cttctgcagg tcgactctcg aggatccggt acccggtatc gatttaaaag gcctatgtac
     2821 ccatacgatg ttccagatta cgcttaccca tacgatgttc cagattacgc ttacccatac
     2881 gatgttccag attacgctta cccatacgat gttccagatt acgcttaccc atacgatgtt
     2941 ccagattacg ctaggcctca ggtcgactct cgaggatcaa ctagtgagct cagtacccgg
     3001 tatcgattta aagaatttcc ccgatcgttc aaacatttgg caataaagtt tcttaagatt
     3061 gaatcctgtt gccggtcttg cgatgattat catataattt ctgttgaatt acgttaagca
     3121 tgtaataatt aacatgtaat gcatgacgtt atttatgaga tgggttttta tgattagagt
     3181 cccgcaatta tacatttaat acgcgataga aaacaaaata tagcgcgcaa actaggataa
     3241 attatcgcgc gcggtgtcat ctatgttact agatcgggaa ttcgtaatca tgtcatagct
     3301 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat
     3361 aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc
     3421 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg
     3481 cgcggggaga ggcggtttgc gtattggcta gagcagcttg ccaacatggt ggagcacgac
     3541 actctcgtct actccaagaa tatcaaagat acagtctcag aagaccaaag ggctattgag
     3601 acttttcaac aaagggtaat atcgggaaac ctcctcggat tccattgccc agctatctgt
     3661 cacttcatca aaaggacagt agaaaaggaa ggtggcacct acaaatgcca tcattgcgat
     3721 aaaggaaagg ctatcgttca agatgcctct gccgacagtg gtcccaaaga tggaccccca
     3781 cccacgagga gcatcgtgga aaaagaagac gttccaacca cgtcttcaaa gcaagtggat
     3841 tgatgtgaac atggtggagc acgacactct cgtctactcc aagaatatca aagatacagt
     3901 ctcagaagac caaagggcta ttgagacttt tcaacaaagg gtaatatcgg gaaacctcct
     3961 cggattccat tgcccagcta tctgtcactt catcaaaagg acagtagaaa aggaaggtgg
     4021 cacctacaaa tgccatcatt gcgataaagg aaaggctatc gttcaagatg cctctgccga
     4081 cagtggtccc aaagatggac ccccacccac gaggagcatc gtggaaaaag aagacgttcc
     4141 aaccacgtct tcaaagcaag tggattgatg tgatatctcc actgacgtaa gggatgacgc
     4201 acaatcccac tatccttcgc aagacccttc ctctatataa ggaagttcat ttcatttgga
     4261 gaggacacgc tgaaatcacc agtctctctc tacaaatcta tctctctcga gctttcgcag
     4321 atccgggggg caatgagata tgaaaaagcc tgaactcacc gcgacgtctg tcgagaagtt
     4381 tctgatcgaa aagttcgaca gcgtctccga cctgatgcag ctctcggagg gcgaagaatc
     4441 tcgtgctttc agcttcgatg taggagggcg tggatatgtc ctgcgggtaa atagctgcgc
     4501 cgatggtttc tacaaagatc gttatgttta tcggcacttt gcatcggccg cgctcccgat
     4561 tccggaagtg cttgacattg gggagtttag cgagagcctg acctattgca tctcccgccg
     4621 ttcacagggt gtcacgttgc aagacctgcc tgaaaccgaa ctgcccgctg ttctacaacc
     4681 ggtcgcggag gctatggatg cgatcgctgc ggccgatctt agccagacga gcgggttcgg
     4741 cccattcgga ccgcaaggaa tcggtcaata cactacatgg cgtgatttca tatgcgcgat
     4801 tgctgatccc catgtgtatc actggcaaac tgtgatggac gacaccgtca gtgcgtccgt
     4861 cgcgcaggct ctcgatgagc tgatgctttg ggccgaggac tgccccgaag tccggcacct
     4921 cgtgcacgcg gatttcggct ccaacaatgt cctgacggac aatggccgca taacagcggt
     4981 cattgactgg agcgaggcga tgttcgggga ttcccaatac gaggtcgcca acatcttctt
     5041 ctggaggccg tggttggctt gtatggagca gcagacgcgc tacttcgagc ggaggcatcc
     5101 ggagcttgca ggatcgccac gactccgggc gtatatgctc cgcattggtc ttgaccaact
     5161 ctatcagagc ttggttgacg gcaatttcga tgatgcagct tgggcgcagg gtcgatgcga
     5221 cgcaatcgtc cgatccggag ccgggactgt cgggcgtaca caaatcgccc gcagaagcgc
     5281 ggccgtctgg accgatggct gtgtagaagt actcgccgat agtggaaacc gacgccccag
     5341 cactcgtccg agggcaaaga aatagagtag atgccgaccg ggatctgtcg atcgacaagc
     5401 tcgagtttct ccataataat gtgtgagtag ttcccagata agggaattag ggttcctata
     5461 gggtttcgct catgtgttga gcatataaga aacccttagt atgtatttgt atttgtaaaa
     5521 tacttctatc aataaaattt ctaattccta aaaccaaaat ccagtactaa aatccagatc
     5581 ccccgaatta attcggcgtt aattcagtac attaaaaacg tccgcaatgt gttattaagt
     5641 tgtctaagcg tcaatttgtt tacaccacaa tatatcctgc caccagccag ccaacagctc
     5701 cccgaccggc agctcggcac aaaatcacca ctcgatacag gcagcccatc agtccgggac
     5761 ggcgtcagcg ggagagccgt tgtaaggcgg cagactttgc tcatgttacc gatgctattc
     5821 ggaagaacgg caactaagct gccgggtttg aaacacggat gatctcgcgg agggtagcat
     5881 gttgattgta acgatgacag agcgttgctg cctgtgatca ccgcggtttc aaaatcggct
     5941 ccgtcgatac tatgttatac gccaactttg aaaacaactt tgaaaaagct gttttctggt
     6001 atttaaggtt ttagaatgca aggaacagtg aattggagtt cgtcttgtta taattagctt
     6061 cttggggtat ctttaaatac tgtagaaaag aggaaggaaa taataaatgg ctaaaatgag
     6121 aatatcaccg gaattgaaaa aactgatcga aaaataccgc tgcgtaaaag atacggaagg
     6181 aatgtctcct gctaaggtat ataagctggt gggagaaaat gaaaacctat atttaaaaat
     6241 gacggacagc cggtataaag ggaccaccta tgatgtggaa cgggaaaagg acatgatgct
     6301 atggctggaa ggaaagctgc ctgttccaaa ggtcctgcac tttgaacggc atgatggctg
     6361 gagcaatctg ctcatgagtg aggccgatgg cgtcctttgc tcggaagagt atgaagatga
     6421 acaaagccct gaaaagatta tcgagctgta tgcggagtgc atcaggctct ttcactccat
     6481 cgacatatcg gattgtccct atacgaatag cttagacagc cgcttagccg aattggatta
     6541 cttactgaat aacgatctgg ccgatgtgga ttgcgaaaac tgggaagaag acactccatt
     6601 taaagatccg cgcgagctgt atgatttttt aaagacggaa aagcccgaag aggaacttgt
     6661 cttttcccac ggcgacctgg gagacagcaa catctttgtg aaagatggca aagtaagtgg
     6721 ctttattgat cttgggagaa gcggcagggc ggacaagtgg tatgacattg ccttctgcgt
     6781 ccggtcgatc agggaggata tcggggaaga acagtatgtc gagctatttt ttgacttact
     6841 ggggatcaag cctgattggg agaaaataaa atattatatt ttactggatg aattgtttta
     6901 gtacctaggg aaggtgcgaa taagcgggga aattcttctc ggctgactca gtcatttcat
     6961 ttcttcatgt ttgagccgat tttttctccc gtaaatgcct tgaatcagcc tatttagacc
     7021 gtttcttcgc catttaaggc gttatcccca gtttttagtg agatctctcc cactgacgta
     7081 tcatttggtc cgcccgaaac aggttggcca gcgtgaataa catcgccagt tggttatcgt
     7141 ttttcagcaa ccccttgtat ctggctttca cgaagccgaa ctgtcgcttg atgatgcgaa
     7201 atgggtgctc caccctggcc cggatgctgg ctttcatgta ttcgatgttg atggccgttt
     7261 tgttcttgcg tggatgctgt ttcaaggttc ttaccttgcc ggggcgctcg gcgatcagcc
     7321 agtccacatc cacctcggcc agctcctcgc gctgtggcgc cccttggtag ccggcatcgg
     7381 ctgagacaaa ttgctcctct ccatgcagca gattacccag ctgattgagg tcatgctcgt
     7441 tggccgcggt ggtgaccagg ctgtgggtca ggccactctt ggcatcgaca ccaatgtggg
     7501 ccttcatgcc aaagtgccac tgattgcctt tcttggtctg atgcatctcc ggatcgcgtt
     7561 gctgctcttt gttcttggtc gagctgggtg cctcaatgat ggtggcatcg accaaggtgc
     7621 cttgagtcat catgacgcct gcttcggcca gccagcgatt gatggtcttg aacaattggc
     7681 gggccagttg atgctgctcc agcaggtggc ggaaattcat gatggtggtg cggtccggca
     7741 aggcgctatc cagggataac cgggcaaaca gacgcatgga ggcgatttcg tacagagcat
     7801 cttccatcgc gccatcgctc aggttgtacc aatgctgcat gcagtgaatg cgtagcatgg
     7861 tttccagcgg ataaggtcgc cggccattac cagccttggg gtaaaacggc tcgatgactt
     7921 ccaccatgtt ttgccatggc agaatctgct ccatgcggga caagaaaatc tcttttctgg
     7981 tctgacggcg cttactgctg aattcactgt cggcgaaggt aagttgatga ctcatgatga
     8041 accctgttct atggctccag atgacaaaca tgatctcata tcagggactt gttcgcacct
     8101 tccctagaat gcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     8161 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     8221 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     8281 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta
     8341 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     8401 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     8461 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     8521 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     8581 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     8641 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     8701 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     8761 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     8821 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
     8881 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg
     8941 agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt
     9001 tcacaccgca tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag
     9061 tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac
     9121 ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga
     9181 ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc
     9241 agggtgcctt gatgtgggcg ccggcggtcg agtggcgacg gcgcggcttg tccgcgccct
     9301 ggtagattgc ctggccgtag gccagccatt tttgagcggc cagcggccgc gataggccga
     9361 cgcgaagcgg cggggcgtag ggagcgcagc gaccgaaggg taggcgcttt ttgcagctct
     9421 tcggctgtgc gctggccaga cagttatgca caggccaggc gggttttaag agttttaata
     9481 agttttaaag agttttaggc ggaaaaatcg ccttttttct cttttatatc agtcacttac
     9541 atgtgtgacc ggttcccaat gtacggcttt gggttcccaa tgtacgggtt ccggttccca
     9601 atgtacggct ttgggttccc aatgtacgtg ctatccacag gaaagagacc ttttcgacct
     9661 ttttcccctg ctagggcaat ttgccctagc atctgctccg tacattagga accggcggat
     9721 gcttcgccct cgatcaggtt gcggtagcgc atgactagga tcgggccagc ctgccccgcc
     9781 tcctccttca aatcgtactc cggcaggtca tttgacccga tcagcttgcg cacggtgaaa
     9841 cagaacttct tgaactctcc ggcgctgcca ctgcgttcgt agatcgtctt gaacaaccat
     9901 ctggcttctg ccttgcctgc ggcgcggcgt gccaggcggt agagaaaacg gccgatgccg
     9961 ggatcgatca aaaagtaatc ggggtgaacc gtcagcacgt ccgggttctt gccttctgtg
    10021 atctcgcggt acatccaatc agctagctcg atctcgatgt actccggccg cccggtttcg
    10081 ctctttacga tcttgtagcg gctaatcaag gcttcaccct cggataccgt caccaggcgg
    10141 ccgttcttgg ccttcttcgt acgctgcatg gcaacgtgcg tggtgtttaa ccgaatgcag
    10201 gtttctacca ggtcgtcttt ctgctttccg ccatcggctc gccggcagaa cttgagtacg
    10261 tccgcaacgt gtggacggaa cacgcggccg ggcttgtctc ccttcccttc ccggtatcgg
    10321 ttcatggatt cggttagatg ggaaaccgcc atcagtacca ggtcgtaatc ccacacactg
    10381 gccatgccgg ccggccctgc ggaaacctct acgtgcccgt ctggaagctc gtagcggatc
    10441 acctcgccag ctcgtcggtc acgcttcgac agacggaaaa cggccacgtc catgatgctg
    10501 cgactatcgc gggtgcccac gtcatagagc atcggaacga aaaaatctgg ttgctcgtcg
    10561 cccttgggcg gcttcctaat cgacggcgca ccggctgccg gcggttgccg ggattctttg
    10621 cggattcgat cagcggccgc ttgccacgat tcaccggggc gtgcttctgc ctcgatgcgt
    10681 tgccgctggg cggcctgcgc ggccttcaac ttctccacca ggtcatcacc cagcgccgcg
    10741 ccgatttgta ccgggccgga tggtttgcga ccgctcacgc cgattcctcg ggcttggggg
    10801 ttccagtgcc attgcagggc cggcaggcaa cccagccgct tacgcctggc caaccgcccg
    10861 ttcctccaca catggggcat tccacggcgt cggtgcctgg ttgttcttga ttttccatgc
    10921 cgcctccttt agccgctaaa attcatctac tcatttattc atttgctcat ttactctggt
    10981 agctgcgcga tgtattcaga tagcagctcg gtaatggtct tgccttggcg taccgcgtac
    11041 atcttcagct tggtgtgatc ctccgccggc aactgaaagt tgacccgctt catggctggc
    11101 gtgtctgcca ggctggccaa cgttgcagcc ttgctgctgc gtgcgctcgg acggccggca
    11161 cttagcgtgt ttgtgctttt gctcattttc tctttacctc attaactcaa atgagttttg
    11221 atttaatttc agcggccagc gcctggacct cgcgggcagc gtcgccctcg ggttctgatt
    11281 caagaacggt tgtgccggcg gcggcagtgc ctgggtagct cacgcgctgc gtgatacggg
    11341 actcaagaat gggcagctcg tacccggcca gcgcctcggc aacctcaccg ccgatgcgcg
    11401 tgcctttgat cgcccgcgac acgacaaagg ccgcttgtag ccttccatcc gtgacctcaa
    11461 tgcgctgctt aaccagctcc accaggtcgg cggtggccca tatgtcgtaa gggcttggct
    11521 gcaccggaat cagcacgaag tcggctgcct tgatcgcgga cacagccaag tccgccgcct
    11581 ggggcgctcc gtcgatcact acgaagtcgc gccggccgat ggccttcacg tcgcggtcaa
    11641 tcgtcgggcg gtcgatgccg acaacggtta gcggttgatc ttcccgcacg gccgcccaat
    11701 cgcgggcact gccctgggga tcggaatcga ctaacagaac atcggccccg gcgagttgca
    11761 gggcgcgggc tagatgggtt gcgatggtcg tcttgcctga cccgcctttc tggttaagta
    11821 cagcgataac cttcatgcgt tccccttgcg tatttgttta tttactcatc gcatcatata
    11881 cgcagcgacc gcatgacgca agctgtttta ctcaaataca catcaccttt ttagacggcg
    11941 gcgctcggtt tcttcagcgg ccaagctggc cggccaggcc gccagcttgg catcagacaa
    12001 accggccagg atttcatgca gccgcacggt tgagacgtgc gcgggcggct cgaacacgta
    12061 cccggccgcg atcatctccg cctcgatctc ttcggtaatg aaaaacggtt cgtcctggcc
    12121 gtcctggtgc ggtttcatgc ttgttcctct tggcgttcat tctcggcggc cgccagggcg
    12181 tcggcctcgg tcaatgcgtc ctcacggaag gcaccgcgcc gcctggcctc ggtgggcgtc
    12241 acttcctcgc tgcgctcaag tgcgcggtac agggtcgagc gatgcacgcc aagcagtgca
    12301 gccgcctctt tcacggtgcg gccttcctgg tcgatcagct cgcgggcgtg cgcgatctgt
    12361 gccggggtga gggtagggcg ggggccaaac ttcacgcctc gggccttggc ggcctcgcgc
    12421 ccgctccggg tgcggtcgat gattagggaa cgctcgaact cggcaatgcc ggcgaacacg
    12481 gtcaacacca tgcggccggc cggcgtggtg gtgtcggccc acggctctgc caggctacgc
    12541 aggcccgcgc cggcctcctg gatgcgctcg gcaatgtcca gtaggtcgcg ggtgctgcgg
    12601 gccaggcggt ctagcctggt cactgtcaca acgtcgccag ggcgtaggtg gtcaagcatc
    12661 ctggccagct ccgggcggtc gcgcctggtg ccggtgatct tctcggaaaa cagcttggtg
    12721 cagccggccg cgtgcagttc ggcccgttgg ttggtcaagt cctggtcgtc ggtgctgacg
    12781 cgggcatagc ccagcaggcc agcggcggcg ctcttgttca tggcgtaatg tctccggttc
    12841 tagtcgcaag tattctactt tatgcgacta aaacacgcga caagaaaacg ccaggaaaag
    12901 ggcagggcgg cagcctgtcg cgtaacttag gacttgtgcg acatgtcgtt ttcagaagac
    12961 ggctgcactg aacgtcagaa gccgactgca ctatagcagc ggaggggttg gatcaaagta
    13021 ctttgatccc gaggggaacc ctgtggttgg catgcacata caaatggacg aacggataaa
    13081 ccttttcacg cccttttaaa tatccgatta ttctaataaa cgctcttttc tcttaggttt
    13141 acccgccaat atatcctgtc aaacactgat agtttaattc ccgatctagt aacatagatg
    13201 acaccgcgcg cgataattta tcctagtttg cgcgctatat tttgttttct atcgcgtatt
    13261 aaatgtataa ttgcgggact ctaatcataa aaacccatct cataaataac gtcatgcatt
    13321 acatgttaat tattacatgc ttaacgtaat tcaacagaaa ttatatgata atcatcgcaa
    13381 gaccggcaac aggattcaat cttaagaaac tttattgcca aatgtttgaa cgatcgggga
    13441 aattcgagct ggtcacctgt aattcacacg tggtggtggt ggtggtggct agcttgtttg
    13501 cctccctgct gcggtttttc accgaagttc atgccagtcc agcgtttttg cagcagaaaa
    13561 gccgccgact tcggtttgcg gtcgcgagtg aagatccctt tcttgttacc gccaacgcgc
    13621 aatatgcctt gcgaggtcgc aaaatcggcg aaattccata cctgttcacc gacgacggcg
    13681 ctgacgcgat caaagacgcg gtgatacata tccagccatg cacactgata ctcttcactc
    13741 cacatgtcgg tgtacattga gtgcagcccg gctaacgtat ccacgccgta ttcggtgatg
    13801 ataatcggct gatgcagttt ctcctgccag gccagaagtt ctttttccag taccttctct
    13861 gccgtttcca aatcgccgct ttggacatac catccgtaat aacggttcag gcacagcaca
    13921 tcaaagagat cgctgatggt atcggtgtga gcgtcgcaga acattacatt gacgcaggtg
    13981 atcggacgcg tcgggtcgag tttacgcgtt gcttccgcca gtggcgcgaa atattcccgt
    14041 gcaccttgcg gacgggtatc cggttcgttg gcaatactcc acatcaccac gcttgggtgg
    14101 tttttgtcac gcgctatcag ctctttaatc gcctgtaagt gcgcttgctg agtttccccg
    14161 ttgactgcct cttcgctgta cagttctttc ggcttgttgc ccgcttcgaa accaatgcct
    14221 aaagagaggt taaagccgac agcagcagtt tcatcaatca ccacgatgcc atgttcatct
    14281 gcccagtcga gcatctcttc agcgtaaggg taatgcgagg tacggtagga gttggcccca
    14341 atccagtcca ttaatgcgtg gtcgtgcacc atcagcacgt tatcgaatcc tttgccacgc
    14401 aagtccgcat cttcatgacg accaaagcca gtaaagtaga acggtttgtg gttaatcagg
    14461 aactgttcgc ccttcactgc cactgaccgg atgccgacgc gaagcgggta gatatcacac
    14521 tctgtctggc ttttggctgt gacgcacagt tcatagagat aaccttcacc cggttgccag
    14581 aggtgcggat tcaccacttg caaagtcccg ctagtgcctt gtccagttgc aaccacctgt
    14641 tgatccgcat cacgcagttc aacgctgaca tcaccattgg ccaccacctg ccagtcaaca
    14701 gacgcgtggt tacagtcttg cgcgacatgc gtcaccacgg tgatatcgtc cacccaggtg
    14761 ttcggcgtgg tgtagagcat tacgctgcga tggattccgg catagttaaa gaaatcatgg
    14821 aagtaagact gctttttctt gccgttttcg tcggtaatca ccattcccgg cgggatagtc
    14881 tgccagttca gttcgttgtt cacacaaacg gtgatacgta cacttttccc ggcaataaca
    14941 tacggcgtga catcggcttc aaatggcgta tagccgccct gatgctccat cacttcctga
    15001 ttattgaccc acactttgcc gtaatgagtg accgcatcga aacgcagcac gatacgctgg
    15061 cctgcccaac ctttcggtat aaagacttcg cgctgatacc agacgttgcc cgcataatta
    15121 cgaatatctg catcggcgaa ctgatcgtta aaactgcctg gcacagcaat tgcccggctt
    15181 tcttgtaacg cgctttccca ccaacgctga tcaattccac agttttcgcg atccagactg
    15241 aatgcccaca ggccgtcgag ttttttgatt tcacgggttg gggtttctac aggacggacg
    15301 agtcgtcggt tctgtaacta tcatcatcat catagacaca cgaaataaag taatcagatt
    15361 atcagttaaa gctatgtaat atttacacca taaccaatca attaaaaaat agatcagttt
    15421 aaagaaagat caaagctcaa aaaaataaaa agagaaaagg gtcctaacca agaaaatgaa
    15481 ggagaaaaac tagaaattta ccctcagatc
//