| 质粒名称: | pMO746 |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 117480 |
| 存储实验室: | Judy Wall |
|
载体骨架基本信息 |
|
| 载体名称: | pCR4/TOPO |
| 载体出品公司: | Invitrogen |
| 空载体大小: | 4000bp |
| 完整质粒大小: | 3205bp |
| 载体类型: | 细菌表达 |
| 载体抗性: | Ampicillin and Kanamycin, 100 & 50 μg/mL |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | uracil 、upp、DVU1025 |
| 物种来源: | Desulfovibrio vulgaris |
| 插入大小: | 627bp |
| GenBank编号 | NC_002937.3 |
|
文献引用方法 |
|
| 材料和方法部分: | pMO746 was a gift from Judy Wall (Addgene plasmid # 117480 ; http://n2t.net/addgene:117480 ; RRID:Addgene_117480) |
| 参考文献部分: | The genetic basis for bacterial mercury methylation. Parks JM, Johs A, Podar M, Bridou R, Hurt RA Jr, Smith SD, Tomanicek SJ, Qian Y, Brown SD, Brandt CC, Palumbo AV, Smith JC, Wall JD, Elias DA, Liang L. Science. 2013 Mar 15;339(6125):1332-5. doi: 10.1126/science.1230667. Epub 2013 Feb 7. 10.1126/science.1230667 PubMed 23393089 |
买家导航
pMO746载体质粒基本信息
pMO746质粒图谱载体图谱和pMO746载体序列质粒序列多克隆位点信息
pMO746质粒载体简介
pMO746质粒序列载体序列
LOCUS pMO746 3206 bp DNA circular SYN 14-APR-2025
DEFINITION upp in an artificial operon with npt from pMO9071 and AmpR-pUC ori
from pCR4/TOPO, Pnpt-npt-upp;, KanR.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 3206)
AUTHORS Parks JM, Johs A, Podar M, Bridou R, Hurt RA Jr, Smith SD, Tomanicek
SJ, Qian Y, Brown SD, Brandt CC, Palumbo AV, Smith JC, Wall JD,
Elias DA, Liang L
TITLE The genetic basis for bacterial mercury methylation.
JOURNAL Science. 2013 Mar 15;339(6125):1332-5. doi: 10.1126/science.1230667.
Epub 2013 Feb 7.
PUBMED 23393089
REFERENCE 2 (bases 1 to 3206)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Apr 14, 2025 from SnapGene Server 8.0.1
https://www.biofeng.com
COMMENT SGRef: number: 1; type: "Journal Article"; doi:
"10.1126/science.1230667"; journalName: "Science"; date:
"2013-03-15- 15"; volume: "339"; issue: "6125"; pages: "1332-5"
COMMENT Sequence Label: pMO746
FEATURES Location/Qualifiers
source 1..3206
/mol_type="other DNA"
/organism="synthetic DNA construct"
CDS complement(join(2..793,794..862))
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 625..644
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 986..1574
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 1475..1494
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(2274..3068)
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(2385..2404)
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
primer_bind 2995..3014
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
ORIGIN
1 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
61 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
121 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
181 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
241 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
301 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
361 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
421 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
481 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
541 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
601 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
661 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
721 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
781 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
841 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
901 gttattgtct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
961 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
1021 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
1081 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
1141 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
1201 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
1261 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
1321 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
1381 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
1441 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
1501 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
1561 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
1621 cttttgctca catcttactt ggtgccgaat atcttgtcgc ccgcgtcgcc gagaccgggc
1681 aggatgtagc cgtgttcgtt caggcgttca tccacggcgg cgacgtagat gtccacatcg
1741 gggtgcttgt ccacgatgcg cttcagccct tcgggcgcgg cgacgaggaa aagtcccttt
1801 atctgcgggc agcctgcatt cttgagaagg tcgatggtgg cgtcgagggt gccgccggtc
1861 gccagcatgg ggtcgaggat gacagccatg cgctcgtcga tgttcttggc gagcttggtg
1921 tagtactgca ccggttcgag ggtctcctcg ttgcggaaca tgcccaccac gctgaccttg
1981 gcaccgggaa tcatatccag gaagccgtcg agcatgccga gaccggcgcg caggatgggc
2041 acggcggtga tcttcttgcc ctttatctgg tcgacctcga caggcccggc ccaaccctgt
2101 acgctgacct tctccgtttc gaggtccttc gtggcctcgt aggcgagaag acggcagagt
2161 tcgttggcga tggcccggaa ttcgctcacc gggacatcat gctggcgcag gcggcccagc
2221 ttgtgtttga ccagcggatg gtcgacgacg taaacagcca cggttgtctc ctttcagaag
2281 aactcgtcaa gaaggcgata gaaggcgatg cgctgcgaat cgggagcggc gataccgtaa
2341 agcacgagga agcggtcagc ccattcgccg ccaagctctt cagcaatatc acgggtagcc
2401 aacgctatgt cctgatagcg gtccgccaca cccagccggc cacagtcgat gaatccagaa
2461 aagcggccat tttccaccat gatattcggc aagcaggcat cgccatgggt cacgacgaga
2521 tcctcgccgt cgggcatgcg cgccttgagc ctggcgaaca gttcggctgg cgcgagcccc
2581 tgatgctctt cgtccagatc atcctgatcg acaagaccgg cttccatccg agtacgtgct
2641 cgctcgatgc gatgtttcgc ttggtggtcg aatgggcagg tagccggatc aagcgtatgc
2701 agccgccgca ttgcatcagc catgatggat actttctcgg caggagcaag gtgagatgac
2761 aggagatcct gccccggcac ttcgcccaat agcagccagt cccttcccgc ttcagtgaca
2821 acgtcgagca cagctgcgca aggaacgccc gtcgtggcca gccacgatag ccgcgctgcc
2881 tcgtcctgca gttcattcag ggcaccggac aggtcggtct tgacaaaaag aaccgggcgc
2941 ccctgcgctg acagccggaa cacggcggca tcagagcagc cgattgtctg ttgtgcccag
3001 tcatagccga atagcctctc cacccaagcg gccggagaac ctgcgtgcaa tccatcttgt
3061 tcaatcatgc gaaacgatcc tcatcctgtc tcttgatcag atcttgatcc cctgcgccat
3121 cagatccttg gcggcaagaa agccatccag tttactttgc agggcttccc aaccttacca
3181 gagggcgccc cagctggcaa ttccgg
//
pMO746载体图谱质粒图谱pdf版和pMO746载体序列质粒序列等相关资料下载
pMO746质粒载体应用举例
- 上一篇：pADH110
- 下一篇：pScaffold-H1 donor
