pAAVLINK-EF1a-HA-5'-HIVEP3

LOCUS       pAAVLINK-EF1a-HA-5'-HIVEP3            7077 bp DNA     circular SYN 20-NOV-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7077)
  AUTHORS   Stavrini Papadaki
  TITLE     Direct Submission
  JOURNAL   Exported Jun 3, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..7077
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     misc_feature    12..130
                     /label=AAV2 ITR
     promoter        158..700
                     /label=EF1-alpha
                     /note="Chimeric promoter of ef1a and htlv 5' UTR"
     promoter        187..398
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             411..679
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     misc_feature    505..510
                     /label=SDS
     CDS             716..742
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             749..4217
                     /codon_start=1
                     /label=HIVEP3
                     /translation="MDPEQSVKGTKKAEGSPRKRLTKGEAIQTSVSSSVPYPGSGTAAT
                     QESPAQELLAPQPFLGPSSVLREGSQEKTGQQQKPPKRPPIEASVHISQLPQHPLTPAF
                     MSPGKPEHLLEGSTWQLVDPMRPGPSGSFVAPGLHPQSQLLPSHASIIPPEDLPGVPKV
                     FVPRPSQVSLKPTEEAHKKERKPQKPGKYICQYCSRPCAKPSVLQKHIRSHTGERPYPC
                     GPCGFSFKTKSNLYKHRKSHAHRIKAGLASGMGGEMYPHGLEMERIPGEEFEEPTEGES
                     TDSEEETSATSGHPAELSPRPKQPLLSSGLYSSGSHSSSHERCSLSQSSTAQSLEDPPP
                     FVEPSSEHPLSHKPEDTHTIKQKLALRLSERKKVIDEQAFLSPGSKGSTESGYFSRSES
                     AEQQVSPPNTNAKSYAEIIFGKCGRIGQRTAMLTATSTQPLLPLSTEDKPSLVPLSVPR
                     TQVIEHITKLITINEAVVDTSEIDSVKPRRSSLSRRSSMESPKSSLYREPLSSHSEKTK
                     PEQSLLSLQHPPSTAPPVPLLRSHSMPSAACTISTPHHPFRGSYSFDDHITDSEALSRS
                     SHVFTSHPRMLKRQPAIELPLGGEYSSEEPGPSSKDTASKPSDEVEPKESELTKKTKKG
                     LKTKGVIYECNICGARYKKRDNYEAHKKYYCSELQIAKPISAGTHTSPEAEKSQIEHEP
                     WSQMMHYKLGTTLELTPLRKRRKEKSLGDEEEPPAFESTKSQFGSPGPSDAARNLPLES
                     TKSPAEPSKSVPSLEGPTGFQPRTPKPGSGSESGKERRTTSKEISVIQHTSSFEKSDSL
                     EQPSGLEGEDKPLAQFPSPPPAPHGRSAHSLQPKLVRQPNIQVPEILVTEEPDRPDTEP
                     EPPPKEPEKTEEFQWPQRSQTLAQLPAEKLPPKKKRLRLAEMAQSSGESSFESSVPLSR
                     SPSQESNVSLSGSSRSASFERDDHGKAEAPSPSSDMRPKPLGTHMLTVPSHHPHAREMR
                     RSASEQSPNVSHSAHMTETRSKSFDYGSLSLTGPSAPAPVAPPARVAPPERRKCFLVRQ
                     ASLSRPPESELEVAPKGRQESEEPQPSSSKPSAKSSLSQISSAATSHGGPPGGKGPGQD
                     RPPLGPTVPYTEALQVFHHPVAQTPLHEKPYLPPPVSLFSFQHLVQHEP"
     misc_feature    2065..2070
                     /label=SDS
     misc_feature    2891..2896
                     /label=SDS
     misc_feature    3042..3047
                     /label=SDS
     intron          4218..4258
                     /label=Splicing Donor
     misc_feature    4262..4295
                     /label=lox2272-JT1571
     repeat_region   4340..4480
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     misc_feature    4340..4469
                     /label=AAV2 ITR
     rep_origin      4555..5010
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        5292..5396
                     /gene="bla"
                     /label=AmpR promoter
     CDS             5397..6257
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      6428..7016
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtgtcgac tctagaggat ctgcgatcgc tccggtgccc
      181 gtcagtgggc agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca
      241 attgaacggg tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact
      301 ggctccgcct ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga
      361 acgttctttt tcgcaacggg tttgccgcca gaacacagct gaagcttcga ggggctcgca
      421 tctctccttc acgcgcccgc cgccctacct gaggccgcca tccacgccgg ttgagtcgcg
      481 ttctgccgcc tcccgcctgt ggtgcctcct gaactgcgtc cgccgtctag gtaagtttaa
      541 agctcaggtc gagaccgggc ctttgtccgg cgctcccttg gagcctacct agactcagcc
      601 ggctctccac gctttgcctg accctgcttg ctcaactcta cgtctttgtt tcgttttctg
      661 ttctgcgccg ttacagatcc aagctgtgac cggcgcctac cgtacggcca ccatgtaccc
      721 ttatgacgta cctgactatg ctggatctat ggatcctgaa caaagtgtca agggcaccaa
      781 gaaggctgag ggaagtcccc ggaagcggct gaccaaagga gaggccattc agaccagtgt
      841 ttcttccagc gtcccatacc caggcagcgg cacagctgcc acccaagaga gccccgccca
      901 agagctctta gccccgcagc ccttcctggg cccctcatca gttcttaggg aaggctctca
      961 ggagaaaacg ggccagcagc agaagccccc caaaaggccc cccatcgaag catccgtcca
     1021 catctcacag cttccgcagc accctctgac accagcattc atgtcgcctg gcaaacctga
     1081 gcatctcctg gaggggtcca catggcaact ggttgacccc atgagacctg gaccctctgg
     1141 ctccttcgtg gcccctgggc tccatcctca gagccagctc cttccttccc acgcttccat
     1201 cattcccccc gaggaccttc ctggagtccc caaagtcttc gtgcctcgtc cttcccaggt
     1261 ctccttgaag cccacagaag aggcacacaa gaaggagagg aagccccaga agccaggcaa
     1321 gtacatctgc cagtactgca gccggccctg tgccaagccc agcgtgctcc agaagcacat
     1381 tcgctcacac acaggtgaga ggccctaccc ctgcggcccc tgtggcttct ccttcaagac
     1441 caagagtaat ctctacaagc acaggaagtc ccatgcccac cgcatcaaag caggcctggc
     1501 ctcaggcatg ggtggcgaga tgtacccaca tgggctggag atggagcgga tccctgggga
     1561 agagtttgag gagcccactg agggagaaag cacagattct gaagaggaga ctagtgccac
     1621 ctctggtcac cctgcagagc tctccccaag acccaagcag ccccttctct ccagcgggct
     1681 atacagctct gggagccaca gttccagcca cgaacgctgt tccctgtccc agtccagcac
     1741 agcccagtca ctcgaagacc cccctccatt tgtggaaccc tcatctgagc accccctgag
     1801 ccataaacct gaagacaccc acacgattaa gcagaagctg gccctccgct taagcgagag
     1861 gaagaaggtg atcgatgagc aggcgtttct gagcccaggc agcaaaggga gtactgagtc
     1921 tgggtatttc tctcgctccg agagtgcaga gcagcaggtc agccccccaa acaccaacgc
     1981 caagtcctac gctgagatca tctttggcaa gtgtgggcga ataggacagc ggaccgccat
     2041 gctgacagcc acctccaccc agcccctcct gcccctctcc accgaagaca agcccagcct
     2101 ggtgcctttg tctgtacccc ggacgcaggt gatcgagcac atcacgaagc tcatcaccat
     2161 caacgaggcc gtggtggaca ccagtgagat cgacagcgtg aagccaaggc ggagctcact
     2221 gtccaggcgc agcagcatgg agtccccaaa atccagcctc taccgggagc ccctgtcatc
     2281 ccacagtgag aaaaccaagc ctgaacaatc actgctgagc ctccagcacc cgcccagtac
     2341 cgccccccct gtgcctctcc tgagaagcca ctcaatgcct tctgccgcct gcactatcag
     2401 caccccccac caccccttcc gaggtagcta ctccttcgat gaccatatca ccgactccga
     2461 agccctgagc cgcagcagtc acgtgtttac ctcccacccc cggatgctga agcgccagcc
     2521 ggcaatcgaa ttacctttgg gaggggaata cagttctgag gagcctggcc caagcagcaa
     2581 agacacagcc tccaagccct cggacgaagt ggaacccaag gaaagcgagc ttaccaaaaa
     2641 gaccaagaag ggtttgaaaa caaaaggggt gatctacgaa tgtaacatat gtggtgctcg
     2701 gtacaagaaa agggataact acgaagccca caaaaaatac tactgctcag agcttcagat
     2761 cgcaaagccc atctctgcag gcacccacac atctccagaa gctgaaaaga gtcagattga
     2821 gcatgagccg tggtcccaaa tgatgcatta caaactggga accaccctgg aactcactcc
     2881 actgaggaag aggaggaaag agaagagcct tggggacgag gaagagccac ctgcctttga
     2941 gtccacaaaa agtcagtttg gcagccccgg gccatctgat gctgctcgga accttcccct
     3001 ggagtccacc aagtcaccag cagaaccaag taaatcagtg ccctccttgg agggacccac
     3061 gggcttccag ccaaggactc ccaagccagg gtccggttca gaatcaggga aggagaggag
     3121 aacaacgtcc aaagaaattt ctgtcatcca gcacaccagc tcctttgaga aatctgattc
     3181 tctcgagcag ccgagtggct tggaagggga agacaaacct ctggcccagt tcccatcacc
     3241 cccacctgcc ccacacggac gctctgctca ctccctgcag cctaagttgg tccgccagcc
     3301 caacattcag gttcctgaga tcctagtaac tgaggagcct gaccggccgg acacagagcc
     3361 agagccgccc cctaaggaac ctgagaagac tgaggagttc caatggcccc agcgcagcca
     3421 gacacttgcc cagctcccag ctgagaagct gccacccaaa aagaagaggt tgcgcctggc
     3481 agagatggcc caatcatcag gggagtccag cttcgagtcc tctgtgcctc tgtctcgcag
     3541 cccgagccag gaaagcaatg tctctttgag tgggtccagc cgctcagcct cgtttgagag
     3601 ggatgaccat gggaaagccg aggcccccag tccctcatct gacatgcgcc ccaaacccct
     3661 gggcacccac atgttgactg tccccagcca ccacccacat gcccgagaga tgcggaggtc
     3721 agcctcagag cagagcccca acgtttccca ttctgcccac atgaccgaga cacgcagcaa
     3781 atcctttgac tatggcagct tgtccttgac aggcccttct gctccagccc cagtggctcc
     3841 accagcgcgg gtggccccgc cagagagaag aaaatgcttc ttggtgagac aggcctctct
     3901 gagcaggcct ccagaatctg agttggaggt tgcccccaag ggaagacagg agagcgaaga
     3961 accacagccc tcatccagta aaccctctgc caaaagctca ttgtcccaga tttcctctgc
     4021 ggccacctca catggtggac ccccgggagg caagggccca gggcaggaca ggcccccatt
     4081 ggggcccact gtgccctaca cagaagcact gcaagtgttc caccaccccg ttgcccagac
     4141 acccctgcat gagaagccat acctgccccc accagtctcc cttttctcct tccagcatct
     4201 cgtgcagcat gagccaggta agtatcaagg ttacaagaca ggtttaagga gaccaataag
     4261 ctattattcg tataggatac tttatacgaa gttattaagt tatggagacc ggtgtggcac
     4321 gtgcggaccg agcggccgca ggaaccccta gtgatggagt tggccactcc ctctctgcgc
     4381 gctcgctcgc tcactgaggc cgggcgacca aaggtcgccc gacgcccggg ctttgcccgg
     4441 gcggcctcag tgagcgagcg agcgcgcagc tgcctgcagg ggcgcctgat gcggtatttt
     4501 ctccttacgc atctgtgcgg tatttcacac cgcatacgtc aaagcaacca tagtacgcgc
     4561 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac
     4621 ttgccagcgc cttagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg
     4681 ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt
     4741 tacggcacct cgaccccaaa aaacttgatt tgggtgatgg ttcacgtagt gggccatcgc
     4801 cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct
     4861 tgttccaaac tggaacaaca ctcaactcta tctcgggcta ttcttttgat ttataaggga
     4921 ttttgccgat ttcggtctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga
     4981 attttaacaa aatattaacg tttacaattt tatggtgcac tctcagtaca atctgctctg
     5041 atgccgcata gttaagccag ccccgacacc cgccaacacc cgctgacgcg ccctgacggg
     5101 cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt
     5161 gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc
     5221 tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc
     5281 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
     5341 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga
     5401 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
     5461 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag
     5521 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag
     5581 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta
     5641 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg
     5701 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca
     5761 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag
     5821 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc
     5881 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg
     5941 tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc
     6001 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg
     6061 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg
     6121 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga
     6181 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac
     6241 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa
     6301 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
     6361 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     6421 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     6481 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     6541 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
     6601 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     6661 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     6721 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     6781 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     6841 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     6901 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     6961 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     7021 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgt
//