pYK325

LOCUS       pYK325           6823 bp DNA     circular SYN 11-FEB-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6823)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 27, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..6823
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    232..819
                     /label=CMV
     primer_bind     863..882
                     /label=T7
     primer_bind     881..900
                     /label=oYK06-ANR-mEGFP-F
     primer_bind     881..900
                     /label=oYK36-AG-Link_s-F
     primer_bind     881..900
                     /label=oYK30-NS3a_Head-F
     misc_feature    905..964
                     /label=NLS2
     primer_bind     932..985
                     /label=oYK30-NS3a_Head-F
     primer_bind     complement(943..987)
                     /label=oYK163_NLS_R
     primer_bind     complement(945..967)
                     /label=oYK54-NLS-R
     primer_bind     complement(945..967)
                     /label=oYK57-NLS-R
     CDS             968..1555
                     /codon_start=1
                     /label=NS3a H1
                     /translation="KKKGSVVIVGRINLSGDTAYSQQTRGLEGCQETSQTGRDKNQVEG
                     EVQVVSTATQSFLATSINGVLWTVYHGAGTRTIASPKGPVTQMYTNVDKDLVGWQAPQG
                     SRSLTPCTCGSSDLYLVTRHADVIPVRGRGDSRGSLLSPRPISYLKGSSGGPLLCPAGH
                     AVGIFRAAVSTRGVAKAVDFIPVESLETTMRSP"
     primer_bind     968..1003
                     /label=oSCL1082
     primer_bind     968..988
                     /label=oyk03-03-F
     primer_bind     968..988
                     /label=oYK04-ns3a-F
     primer_bind     968..986
                     /label=oYK25-NS3a-F
     primer_bind     985..1043
                     /label=oSCL1083
     primer_bind     1024..1082
                     /label=oSCL1084
     primer_bind     1061..1119
                     /label=oSCL1085
     primer_bind     1098..1156
                     /label=oSCL1086
     primer_bind     complement(1126..1145)
                     /label=oSCL1106
     primer_bind     1127..1165
                     /label=oSCL1104
     primer_bind     complement(1362..1420)
                     /label=oSCL1105
     primer_bind     complement(1402..1473)
                     /label=oSCL1087
     primer_bind     1404..1425
                     /label=oSCL1107
     primer_bind     1455..1473
                     /label=oSCL1088
     primer_bind     complement(1537..1565)
                     /label=oYK1077_NS3a_R
     primer_bind     complement(1537..1561)
                     /label=oSCL1089
     primer_bind     complement(1540..1572)
                     /label=oYK30-NS3a_head-R
     primer_bind     complement(1541..1562)
                     /label=oYK1075_NS3a_R
     primer_bind     complement(1543..1584)
                     /label=oYK66-NS3a-R
     primer_bind     complement(1544..1561)
                     /label=oYK1076_NS3Flag_R
     misc_feature    1562..1572
                     /label=linker
     CDS             1565..1639
                     /codon_start=1
                     /label=5xGS Linker
                     /translation="GSGTGSGTGSGTGTTSGTGTGGSTG"
     primer_bind     1565..1585
                     /label=oYK66-AG-lplA-F
     primer_bind     1565..1583
                     /label=Primer 1
     primer_bind     complement(1614..1642)
                     /label=olyl212
     primer_bind     complement(1614..1639)
                     /label=olyl234
     primer_bind     complement(1623..1659)
                     /label=oYK45-46 ANR1-R
     primer_bind     complement(1623..1639)
                     /label=oYK1165_NS3a_R
     primer_bind     complement(1638..1659)
                     /label=oYK1075_NS3a_R
     primer_bind     1639..1658
                     /label=oYK36-AG-Link_s-F
     primer_bind     1640..1659
                     /label=oYK41-44 AG-LplA-F
     primer_bind     1640..1659
                     /label=oYK45-46 AG-lplA-F
     primer_bind     complement(1640..1659)
                     /label=oYK1076_NS3Flag_R
     misc_feature    1640..1642
                     /label=Long_Linker
     misc_feature    1643..2317
                     /label=AG
     primer_bind     2091..2101
                     /label=oSCL1104
     primer_bind     complement(2302..2317)
                     /label=oYK37-AG-R
     primer_bind     complement(2303..2340)
                     /label=oYK224_AG_R
     primer_bind     complement(2321..2343)
                     /label=oYK57-MCP-mCherry-R
     primer_bind     complement(2345..2364)
                     /label=oSCL1089
     terminator      2417..2644
                     /label=bGH PA
     misc_feature    2690..3118
                     /label=f1 origin
     misc_feature    3123..3466
                     /label=SV40 early promoter and origin
     misc_feature    3312..3389
                     /label=SV40 ORI
     CDS             3531..4322
                     /label=Kan/neoR
     misc_feature    4496..4626
                     /label=SV40 early polyadenylation signal
     primer_bind     complement(4662..4682)
                     /label=M13-rev
     misc_binding    complement(4688..4710)
                     /label=LacO
     promoter        complement(4715..4744)
                     /label=lac
     misc_feature    complement(5009..5682)
                     /label=pUC origin
     CDS             complement(5830..6489)
                     /label=AmpR
     primer_bind     6146..6157
                     /label=oyk03-03-F
     primer_bind     6146..6157
                     /label=oYK04-ns3a-F
     promoter        complement(6729..6757)
                     /label=Amp prom
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 caccatgccc aagaagaagc gcaaggtgga agctagcgct tccccgaaga aaaagcggaa
      961 agtcatgaag aagaagggca gcgtggtgat cgtgggcaga atcaacctga gcggcgacac
     1021 cgcctacagc cagcagacca gaggcctgga gggctgccag gagaccagcc agaccggcag
     1081 agacaagaac caggtggagg gcgaggtgca ggtggtgagc accgccaccc agagcttcct
     1141 ggccaccagc atcaacggcg tgctgtggac cgtgtaccac ggcgccggca ccagaaccat
     1201 cgccagcccc aagggccccg tgacccagat gtacacgaac gtggacaagg acttagtggg
     1261 ctggcaggcc ccccaaggca gcagaagcct gaccccctgc acctgcggca gcagcgacct
     1321 gtacctggtg accagacacg ccgacgtgat ccccgtgaga ggaagaggcg acagcagagg
     1381 cagcctgctg agccccagac ccatcagcta tctgaaaggc tcaagcggcg gccccctgtt
     1441 gtgtcccgcc ggccatgccg tgggcatctt cagagccgcc gtgagcacca gaggcgtggc
     1501 caaggccgtg gacttcatcc ccgtggagag cctggagacc accatgagaa gccccgctag
     1561 cagcggaagt ggaactgggt ctgggactgg gtccggcacc gggactactt caggcaccgg
     1621 cactgggggc tccactggca tggtgagcgt gatcaagccc gagatgaaga tcaagctgtg
     1681 catgaggggc accgtgaacg gccacaactt cgtgatcgag ggcgagggca agggcaaccc
     1741 ctacgagggc acccagatcc tggacctgaa cgtgaccgag ggcgcccccc tgcccttcgc
     1801 ctacgacatc ctgaccaccg tgttccagta cggcaacagg gccttcacca agtaccccgc
     1861 cgacatccag gactacttca agcagacctt ccccgagggc taccactggg agaggagcat
     1921 gacctacgag gaccagggca tctgcaccgc caccagcaac atcagcatga ggggcgactg
     1981 cttcttctac gacatcaggt tcgacggcgt gaacttcccc cccaacggcc ccgtgatgca
     2041 gaagaagacc ctgaagtggg agcccagcac cgagaagatg tacgtgaggg acggcgtgct
     2101 gaagggcgac gtgaacatgg ccctgctgct ggagggcggc ggccactaca ggtgcgactt
     2161 caagaccacc tacaaggcca agaaggacgt gaggctgccc gactaccact tcgtggacca
     2221 caggatcgag atcctgaagc acgacaagga ctacaacaag gtgaagctgt acgagaacgc
     2281 cgtggccagg tacagcatgc tgcccagcca ggccaagtaa ggatccacta gtccagtgtg
     2341 gtggaattct gcagatatcc agcacagtgg cggccgctcg agtctagagg gcccgtttaa
     2401 acccgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc
     2461 cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag
     2521 gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag
     2581 gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct
     2641 atggcttctg aggcggaaag aaccagctgg ggctctaggg ggtatcccca cgcgccctgt
     2701 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
     2761 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
     2821 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg
     2881 cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga
     2941 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc
     3001 caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg
     3061 ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaattaa
     3121 ttctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa
     3181 gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc
     3241 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc
     3301 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct
     3361 gactaatttt ttttatttat gcagaggccg aggccgcctc tgcctctgag ctattccaga
     3421 agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctcccg ggagcttgta
     3481 tatccatttt cggatctgat caagagacag gatgaggatc gtttcgcatg attgaacaag
     3541 atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg
     3601 cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc
     3661 cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag gacgaggcag
     3721 cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca
     3781 ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat
     3841 ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata
     3901 cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac
     3961 gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc
     4021 tcgcgccagc cgaactgttc gccaggctca aggcgcgcat gcccgacggc gaggatctcg
     4081 tcgtgaccca tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg
     4141 gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta
     4201 cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg
     4261 gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct
     4321 gagcgggact ctggggttcg aaatgaccga ccaagcgacg cccaacctgc catcacgaga
     4381 tttcgattcc accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc
     4441 cggctggatg atcctccagc gcggggatct catgctggag ttcttcgccc accccaactt
     4501 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
     4561 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
     4621 tgtctgtata ccgtcgacct ctagctagag cttggcgtaa tcatggtcat agctgtttcc
     4681 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg
     4741 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc
     4801 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg
     4861 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
     4921 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
     4981 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
     5041 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     5101 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     5161 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     5221 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     5281 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     5341 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     5401 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     5461 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
     5521 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     5581 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     5641 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     5701 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
     5761 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
     5821 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
     5881 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
     5941 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
     6001 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
     6061 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
     6121 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
     6181 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
     6241 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
     6301 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
     6361 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
     6421 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
     6481 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
     6541 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
     6601 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
     6661 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
     6721 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
     6781 aggggttccg cgcacatttc cccgaaaagt gccacctgac gtc
//