| 质粒名称: | pYSJ49 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0002579 |
| 产品用途: | Synthetic mammalian ETS TF-dependent SEAP expression vector ((CCGGAAGT)3-PhCMVmin-SEAP-pA). |
| 存储实验室: | Mingqi XIE Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pcDNA3.1 |
| 表达系统: | 哺乳动物表达 |
| 启动子: | Synthetic |
| 拷贝数: | 低拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | Stbl3 |
|
插入基因信息 |
|
| 插入基因名称: | SEAP |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
pYSJ49
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pYSJ49载体质粒基本信息
pYSJ49质粒图谱载体图谱和pYSJ49载体序列质粒序列多克隆位点信息
pYSJ49质粒载体简介
pYSJ49质粒序列载体序列
LOCUS pYSJ49 6430 bp DNA circular SYN 14-JAN-2024
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6430)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Sep 19, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6430
/mol_type="other DNA"
/organism="synthetic DNA construct"
primer_bind 214..273
/label=oYSJ145-X3-F
primer_bind complement(214..273)
/label=oYSJ146-X3-R
primer_bind 214..253
/label=oYSJ173-X3-F
primer_bind complement(234..273)
/label=oYSJ174-X3-R
misc_feature 234..241
/label=(CCGGAA)X1
misc_feature 242..249
/label=X2
misc_feature 250..257
/label=X3
misc_feature 264..411
/label=hcmvmin
misc_feature 333..347
/label=New Feature
misc_feature 434..457
/label=New Feature
misc_feature 459..1979
/label=SEAP
terminator 2027..2254
/label=bGH PA
misc_feature 2922..2999
/label=SV40 ORI
CDS 3141..3932
/label=Kan/neoR
primer_bind complement(4272..4292)
/label=M13-rev
misc_binding complement(4298..4320)
/label=LacO
promoter complement(4325..4354)
/label=lac
CDS complement(5437..6096)
/label=AmpR
promoter complement(6336..6364)
/label=Amp prom
ORIGIN
1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgtccggaag
241 tccggaagtc cggaagtgct agctcgagct cggtacccgg gtcgagtagg cgtgtacggt
301 gggaggccta tataagcaga gctcgtttag tgaaccgtca gatcgcctgg agacgccatc
361 cacgctgttt tgacctccat agaagacacc gggaccgatc cagcctccgc ggccccgaat
421 tcgagctcgc ccggggatcc tctagagtca gcttctgcat gctgctgctg ctgctgctgc
481 tgggcctgag gctacagctc tccctgggca tcatcccagt tgaggaggag aacccggact
541 tctggaaccg cgaggcagcc gaggccctgg gtgccgccaa gaagctgcag cctgcacaga
601 cagccgccaa gaacctcatc atcttcctgg gcgatgggat gggggtgtct acggtgacag
661 ctgccaggat cctaaaaggg cagaagaagg acaaactggg gcctgagata cccctggcca
721 tggaccgctt cccatatgtg gctctgtcca agacatacaa tgtagacaaa catgtgccag
781 acagtggagc cacagccacg gcctacctgt gcggggtcaa gggcaacttc cagaccattg
841 gcttgagtgc agccgcccgc tttaaccagt gcaacacgac acgcggcaac gaggtcatct
901 ccgtgatgaa tcgggccaag aaagcaggga agtcagtggg agtggtaacc accacacgag
961 tgcagcacgc ctcgccagcc ggcacctacg cccacacggt gaaccgcaac tggtactcgg
1021 acgccgacgt gcctgcctcg gcccgccagg aggggtgcca ggacatcgct acgcagctca
1081 tctccaacat ggacattgac gtgatcctag gtggaggccg aaagtacatg tttcccatgg
1141 gaaccccaga ccctgagtac ccagatgact acagccaagg tgggaccagg ctggacggga
1201 agaatctggt gcaggaatgg ctggcgaagc gccagggtgc ccggtatgtg tggaaccgca
1261 ctgagctcat gcaggcttcc ctggacccgt ctgtgaccca tctcatgggt ctctttgagc
1321 ctggagacat gaaatacgag atccaccgag actccacact ggacccctcc ctgatggaga
1381 tgacagaggc tgccctgcgc ctgctgagca ggaacccccg cggcttcttc ctcttcgtgg
1441 agggtggtcg catcgaccat ggtcatcatg aaagcagggc ttaccgggca ctgactgaga
1501 cgatcatgtt cgacgacgcc attgagaggg cgggccagct caccagcgag gaggacacgc
1561 tgagcctcgt cactgccgac cactcccacg tcttctcctt cggaggctac cccctgcgag
1621 ggagctccat cttcgggctg gcccctggca aggcccggga caggaaggcc tacacggtcc
1681 tcctatacgg aaacggtcca ggctatgtgc tcaaggacgg cgcccggccg gatgttaccg
1741 agagcgagag cgggagcccc gagtatcggc agcagtcagc agtgcccctg gacgaagaga
1801 cccacgcagg cgaggacgtg gcggtgttcg cgcgcggccc gcaggcgcac ctggttcacg
1861 gcgtgcagga gcagaccttc atagcgcacg tcatggcctt cgccgcctgc ctggagccct
1921 acaccgcctg cgacctggcg ccccccgccg gcaccaccga cgccgcgcac ccgggttaag
1981 cggccgctcg agtctagagg gcccgtttaa acccgctgat cagcctcgac tgtgccttct
2041 agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc
2101 actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt
2161 cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat
2221 agcaggcatg ctggggatgc ggtgggctct atggcttctg aggcggaaag aaccagctgg
2281 ggctctaggg ggtatcccca cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
2341 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc
2401 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc
2461 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt
2521 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag
2581 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg
2641 gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag
2701 ctgatttaac aaaaatttaa cgcgaattaa ttctgtggaa tgtgtgtcag ttagggtgtg
2761 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
2821 caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
2881 tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc
2941 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg
3001 aggccgcctc tgcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag
3061 gcttttgcaa aaagctcccg ggagcttgta tatccatttt cggatctgat caagagacag
3121 gatgaggatc gtttcgcatg attgaacaag atggattgca cgcaggttct ccggccgctt
3181 gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg
3241 ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg
3301 gtgccctgaa tgaactgcag gacgaggcag cgcggctatc gtggctggcc acgacgggcg
3361 ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg
3421 gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca
3481 tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc
3541 accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc
3601 aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca
3661 aggcgcgcat gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga
3721 atatcatggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg
3781 cggaccgcta tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg
3841 aatgggctga ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg
3901 ccttctatcg ccttcttgac gagttcttct gagcgggact ctggggttcg aaatgaccga
3961 ccaagcgacg cccaacctgc catcacgaga tttcgattcc accgccgcct tctatgaaag
4021 gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct
4081 catgctggag ttcttcgccc accccaactt gtttattgca gcttataatg gttacaaata
4141 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
4201 tttgtccaaa ctcatcaatg tatcttatca tgtctgtata ccgtcgacct ctagctagag
4261 cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc
4321 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta
4381 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca
4441 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
4501 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
4561 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
4621 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
4681 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
4741 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
4801 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
4861 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
4921 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
4981 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
5041 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
5101 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt
5161 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtttttt
5221 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
5281 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
5341 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
5401 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
5461 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
5521 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
5581 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
5641 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
5701 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
5761 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
5821 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
5881 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
5941 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
6001 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
6061 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
6121 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
6181 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
6241 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
6301 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
6361 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
6421 acctgacgtc
//
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