| 质粒名称: | pYK464 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0000816 |
| 产品用途: | Mammalian PhCMV-driven constitutive dCas9-NS3a(H1) expression vector (PhCMV-dCas9-NS3a(H1)-pA). |
| 存储实验室: | Anping ZENG Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pCMV |
| 表达系统: | 哺乳动物表达 |
| 启动子: | CMV |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | Stbl3 |
|
插入基因信息 |
|
| 插入基因名称: | dCas9-NS3a(H1) |
| 物种来源: | synthetic |
| 密码子优化: | 哺乳细胞表达优化 |
|
文献引用方法 |
|
| 参考文献部分: |
Programmable solid-state condensates for spatiotemporal control of mammalian gene expression
Wang Y., Jiang J., Xiong Q., Li S., Shao J., Xie M., Zeng A.P.
Nat. Chem. Biol. 2025 1–10 (2025)
|
pYK464
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pYK464载体质粒基本信息
pYK464质粒图谱载体图谱和pYK464载体序列质粒序列多克隆位点信息
pYK464质粒载体简介
pYK464质粒序列载体序列
LOCUS pYK464 10215 bp DNA circular SYN 11-FEB-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 10215)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Jul 27, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..10215
/mol_type="other DNA"
/organism="synthetic DNA construct"
misc_feature 232..819
/label=CMV
primer_bind 863..882
/label=T7
primer_bind 880..899
/label=oSCL1162
primer_bind 881..919
/label=oYK207_dcas9_F
CDS 920..5059
/label=Translation 748-6546
CDS 920..5023
/label=dCas9 (D839A and N863A nonsense-mutation)
primer_bind complement(5036..5079)
/label=oYK464_dCas9_2_R
primer_bind complement(5036..5059)
/label=oYK464_dCas9_R
misc_feature 5036..5056
/label=NLS (SV40 short)
/label=nonstandard type: signal
misc_feature 5060..5095
/label=linker
CDS 5060..5089
/codon_start=1
/label=linker
/translation="ASGSGGGGDV"
primer_bind 5060..5089
/label=oSCL388
primer_bind 5060..5079
/label=oLZ048 NSP3-F
primer_bind 5060..5079
/label=oSCL1192
primer_bind 5060..5079
/label=oSCL1129
primer_bind complement(5060..5079)
/label=oSCL1163
primer_bind 5060..5077
/label=oLZ147 NS3a F
primer_bind 5060..5076
/label=oYF0033
primer_bind 5060..5076
/label=oYK419_GS_F
primer_bind 5060..5075
/label=oYK28-mCherry-NS3a-F
primer_bind complement(5067..5085)
/label=Oscl18-4
primer_bind 5070..5114
/label=oSCL1502
primer_bind complement(5070..5109)
/label=oSCL1119
primer_bind complement(5070..5089)
/label=oSCL075
primer_bind complement(5070..5089)
/label=oSCL075a
primer_bind complement(5070..5089)
/label=oSCL075a
primer_bind 5073..5131
/label=oSCL1082
misc_feature 5073..5080
/label=linker
CDS 5096..5683
/codon_start=1
/label=NS3a H1
/translation="KKKGSVVIVGRINLSGDTAYSQQTRGLEGCQETSQTGRDKNQVEG
EVQVVSTATQSFLATSINGVLWTVYHGAGTRTIASPKGPVTQMYTNVDKDLVGWQAPQG
SRSLTPCTCGSSDLYLVTRHADVIPVRRRGDSRGSLLSPRPISYLKGSSGGPLLCPAGH
AVGIFRAAVSTRGVAKAVDFIPVESLETTMRSP"
primer_bind 5096..5114
/label=oSCL1162
primer_bind 5113..5171
/label=oSCL1083
primer_bind 5152..5210
/label=oSCL1084
primer_bind 5189..5247
/label=oSCL1085
primer_bind 5226..5284
/label=oSCL1086
primer_bind complement(5254..5273)
/label=oSCL1106
primer_bind 5255..5293
/label=oSCL1104
primer_bind complement(5490..5548)
/label=oSCL1105
primer_bind complement(5530..5601)
/label=oSCL1087
primer_bind 5532..5553
/label=oSCL1107
primer_bind 5583..5601
/label=oSCL1088
primer_bind complement(5665..5712)
/label=oSCL1089
primer_bind complement(5665..5712)
/label=oSCL1503
primer_bind complement(5665..5712)
/label=oSCL1532
primer_bind complement(5665..5689)
/label=oSCL1163
primer_bind 5669..5688
/label=oYF0033
primer_bind 5669..5688
/label=oSCL1192
primer_bind 5669..5688
/label=oSCL1129
primer_bind 5670..5688
/label=oLZ147 NS3a F
primer_bind complement(5684..5732)
/label=oYK03-03-R
primer_bind complement(5684..5703)
/label=oSCL1130
primer_bind complement(5735..5755)
/label=oSCL1503
primer_bind complement(5737..5756)
/label=oSCL1089
primer_bind complement(5737..5756)
/label=oSCL1532
terminator 5809..6036
/label=bGH PA
misc_feature 6082..6510
/label=f1 origin
misc_feature 6515..6858
/label=SV40 early promoter and origin
misc_feature 6704..6781
/label=SV40 ORI
CDS 6923..7714
/label=Kan/neoR
misc_feature 7888..8018
/label=SV40 early polyadenylation signal
primer_bind complement(8054..8074)
/label=M13-rev
misc_binding complement(8080..8102)
/label=LacO
promoter complement(8107..8136)
/label=lac
misc_feature complement(8401..9074)
/label=pUC origin
CDS complement(9222..9881)
/label=AmpR
promoter complement(10121..10149)
/label=Amp prom
ORIGIN
1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
901 ggatcgaacc cttgccacca tggacaagaa gtactccatt gggctcgcta tcggcacaaa
961 cagcgtcggc tgggccgtca ttacggacga gtacaaggtg ccgagcaaaa aattcaaagt
1021 tctgggcaat accgatcgcc acagcataaa gaagaacctc attggcgccc tcctgttcga
1081 ctccggggag acggccgaag ccacgcggct caaaagaaca gcacggcgca gatatacccg
1141 cagaaagaat cggatctgct acctgcagga gatctttagt aatgagatgg ctaaggtgga
1201 tgactctttc ttccataggc tggaggagtc ctttttggtg gaggaggata aaaagcacga
1261 gcgccaccca atctttggca atatcgtgga cgaggtggcg taccatgaaa agtacccaac
1321 catatatcat ctgaggaaga agcttgtaga cagtactgat aaggctgact tgcggttgat
1381 ctatctcgcg ctggcgcata tgatcaaatt tcggggacac ttcctcatcg agggggacct
1441 gaacccagac aacagcgatg tcgacaaact ctttatccaa ctggttcaga cttacaatca
1501 gcttttcgaa gagaacccga tcaacgcatc cggagttgac gccaaagcaa tcctgagcgc
1561 taggctgtcc aaatcccggc ggctcgaaaa cctcatcgca cagctccctg gggagaagaa
1621 gaacggcctg tttggtaatc ttatcgccct gtcactcggg ctgaccccca actttaaatc
1681 taacttcgac ctggccgaag atgccaagct tcaactgagc aaagacacct acgatgatga
1741 tctcgacaat ctgctggccc agatcggcga ccagtacgca gacctttttt tggcggcaaa
1801 gaacctgtca gacgccattc tgctgagtga tattctgcga gtgaacacgg agatcaccaa
1861 agctccgctg agcgctagta tgatcaagcg ctatgatgag caccaccaag acttgacttt
1921 gctgaaggcc cttgtcagac agcaactgcc tgagaagtac aaggaaattt tcttcgatca
1981 gtctaaaaat ggctacgccg gatacattga cggcggagca agccaggagg aattttacaa
2041 atttattaag cccatcttgg aaaaaatgga cggcaccgag gagctgctgg taaagcttaa
2101 cagagaagat ctgttgcgca aacagcgcac tttcgacaat ggaagcatcc cccaccagat
2161 tcacctgggc gaactgcacg ctatcctcag gcggcaagag gatttctacc cctttttgaa
2221 agataacagg gaaaagattg agaaaatcct cacatttcgg ataccctact atgtaggccc
2281 cctcgcccgg ggaaattcca gattcgcgtg gatgactcgc aaatcagaag agaccatcac
2341 tccctggaac ttcgaggaag tcgtggataa gggggcctct gcccagtcct tcatcgaaag
2401 gatgactaac tttgataaaa atctgcctaa cgaaaaggtg cttcctaaac actctctgct
2461 gtacgagtac ttcacagttt ataacgagct caccaaggtc aaatacgtca cagaagggat
2521 gagaaagcca gcattcctgt ctggagagca gaagaaagct atcgtggacc tcctcttcaa
2581 gacgaaccgg aaagttaccg tgaaacagct caaagaagac tatttcaaaa agattgaatg
2641 tttcgactct gttgaaatca gcggagtgga ggatcgcttc aacgcatccc tgggaacgta
2701 tcacgatctc ctgaaaatca ttaaagacaa ggacttcctg gacaatgagg agaacgagga
2761 cattcttgag gacattgtcc tcacccttac gttgtttgaa gatagggaga tgattgaaga
2821 acgcttgaaa acttacgctc atctcttcga cgacaaagtc atgaaacagc tcaagaggcg
2881 ccgatataca ggatgggggc ggctgtcaag aaaactgatc aatgggatcc gagacaagca
2941 gagtggaaag acaatcctgg attttcttaa gtccgatgga tttgccaacc ggaacttcat
3001 gcagttgatc catgatgact ctctcacctt taaggaggac atccagaaag cacaagtttc
3061 tggccagggg gacagtcttc acgagcacat cgctaatctt gcaggtagcc cagctatcaa
3121 aaagggaata ctgcagaccg ttaaggtcgt ggatgaactc gtcaaagtaa tgggaaggca
3181 taagcccgag aatatcgtta tcgagatggc ccgagagaac caaactaccc agaagggaca
3241 gaagaacagt agggaaagga tgaagaggat tgaagagggt ataaaagaac tggggtccca
3301 aatccttaag gaacacccag ttgaaaacac ccagcttcag aatgagaagc tctacctgta
3361 ctacctgcag aacggcaggg acatgtacgt ggatcaggaa ctggacatca atcggctctc
3421 cgactacgac gtggctgcta tcgtgcccca gtcttttctc aaagatgatt ctattgataa
3481 taaagtgttg acaagatccg ataaagctag agggaagagt gataacgtcc cctcagaaga
3541 agttgtcaag aaaatgaaaa attattggcg gcagctgctg aacgccaaac tgatcacaca
3601 acggaagttc gataatctga ctaaggctga acgaggtggc ctgtctgagt tggataaagc
3661 cggcttcatc aaaaggcagc ttgttgagac acgccagatc accaagcacg tggcccaaat
3721 tctcgattca cgcatgaaca ccaagtacga tgaaaatgac aaactgattc gagaggtgaa
3781 agttattact ctgaagtcta agctggtctc agatttcaga aaggactttc agttttataa
3841 ggtgagagag atcaacaatt accaccatgc gcatgatgcc tacctgaatg cagtggtagg
3901 cactgcactt atcaaaaaat atcccaagct tgaatctgaa tttgtttacg gagactataa
3961 agtgtacgat gttaggaaaa tgatcgcaaa gtctgagcag gaaataggca aggccaccgc
4021 taagtacttc ttttacagca atattatgaa ttttttcaag accgagatta cactggccaa
4081 tggagagatt cggaagcgac cacttatcga aacaaacgga gaaacaggag aaatcgtgtg
4141 ggacaagggt agggatttcg cgacagtccg gaaggtcctg tccatgccgc aggtgaacat
4201 cgttaaaaag accgaagtac agaccggagg cttctccaag gaaagtatcc tcccgaaaag
4261 gaacagcgac aagctgatcg cacgcaaaaa agattgggac cccaagaaat acggcggatt
4321 cgattctcct acagtcgctt acagtgtact ggttgtggcc aaagtggaga aagggaagtc
4381 taaaaaactc aaaagcgtca aggaactgct gggcatcaca atcatggagc gatcaagctt
4441 cgaaaaaaac cccatcgact ttctcgaggc gaaaggatat aaagaggtca aaaaagacct
4501 catcattaag cttcccaagt actctctctt tgagcttgaa aacggccgga aacgaatgct
4561 cgctagtgcg ggcgagctgc agaaaggtaa cgagctggca ctgccctcta aatacgttaa
4621 tttcttgtat ctggccagcc actatgaaaa gctcaaaggg tctcccgaag ataatgagca
4681 gaagcagctg ttcgtggaac aacacaaaca ctaccttgat gagatcatcg agcaaataag
4741 cgaattctcc aaaagagtga tcctcgccga cgctaacctc gataaggtgc tttctgctta
4801 caataagcac agggataagc ccatcaggga gcaggcagaa aacattatcc acttgtttac
4861 tctgaccaac ttgggcgcgc ctgcagcctt caagtacttc gacaccacca tagacagaaa
4921 gcggtacacc tctacaaagg aggtcctgga cgccacactg attcatcagt caattacggg
4981 gctctatgaa acaagaatcg acctctctca gctcggtgga gacagcaggg ctgaccccaa
5041 gaagaagagg aaggtgtcgg ccagcggaag tggcggagga ggcgacgtcg gatccaagaa
5101 gaagggcagc gtggtgatcg tgggcagaat caacctgagc ggcgacaccg cctacagcca
5161 gcagaccaga ggcctggagg gctgccagga gaccagccag accggcagag acaagaacca
5221 ggtggagggc gaggtgcagg tggtgagcac cgccacccag agcttcctgg ccaccagcat
5281 caacggcgtg ctgtggaccg tgtaccacgg cgccggcacc agaaccatcg ccagccccaa
5341 gggccccgtg acccagatgt acacgaacgt ggacaaggac ttagtgggct ggcaggcccc
5401 ccaaggcagc agaagcctga ccccctgcac ctgcggcagc agcgacctgt acctggtgac
5461 cagacacgcc gacgtgatcc ccgtgagaag aagaggcgac agcagaggca gcctgctgag
5521 ccccagaccc atcagctatc tgaaaggctc aagcggcggc cccctgttgt gtcccgccgg
5581 ccatgccgtg ggcatcttca gagccgccgt gagcaccaga ggcgtggcca aggccgtgga
5641 cttcatcccc gtggagagcc tggagaccac catgagaagc cccgctagct agaaattctg
5701 cagatatcca gcggatccac tagtccagtg tggtggaatt ctgcagatat ccagcacagt
5761 ggcggccgct cgagtctaga gggcccgttt aaacccgctg atcagcctcg actgtgcctt
5821 ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg
5881 ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt
5941 gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca
6001 atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa agaaccagct
6061 ggggctctag ggggtatccc cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg
6121 tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt
6181 tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc
6241 tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg
6301 gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg
6361 agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct
6421 cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg
6481 agctgattta acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg
6541 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
6601 agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
6661 tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc
6721 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc
6781 cgaggccgcc tctgcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct
6841 aggcttttgc aaaaagctcc cgggagcttg tatatccatt ttcggatctg atcaagagac
6901 aggatgagga tcgtttcgca tgattgaaca agatggattg cacgcaggtt ctccggccgc
6961 ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc
7021 cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc
7081 cggtgccctg aatgaactgc aggacgaggc agcgcggcta tcgtggctgg ccacgacggg
7141 cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt
7201 gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc
7261 catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga
7321 ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga
7381 tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct
7441 caaggcgcgc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc
7501 gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt
7561 ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg
7621 cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat
7681 cgccttctat cgccttcttg acgagttctt ctgagcggga ctctggggtt cgaaatgacc
7741 gaccaagcga cgcccaacct gccatcacga gatttcgatt ccaccgccgc cttctatgaa
7801 aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca gcgcggggat
7861 ctcatgctgg agttcttcgc ccaccccaac ttgtttattg cagcttataa tggttacaaa
7921 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
7981 ggtttgtcca aactcatcaa tgtatcttat catgtctgta taccgtcgac ctctagctag
8041 agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt
8101 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc
8161 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc
8221 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct
8281 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
8341 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
8401 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
8461 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
8521 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
8581 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
8641 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
8701 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
8761 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
8821 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
8881 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
8941 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
9001 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
9061 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
9121 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
9181 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
9241 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
9301 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
9361 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
9421 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
9481 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
9541 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
9601 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
9661 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
9721 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
9781 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
9841 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
9901 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
9961 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
10021 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
10081 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
10141 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
10201 gtgccacctg acgtc
//
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