| 质粒名称: | pYK154 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0000793 |
| 产品用途: | Mammalian PhCMV-driven constitutive G3BP1-AG-NS3a(H1) expression vector (PhCMV-G3BP1-AG-NS3a(H1)-pA). |
| 存储实验室: | Anping ZENG Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pCMV |
| 表达系统: | 哺乳动物表达 |
| 启动子: | CMV |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | Stbl3 |
|
插入基因信息 |
|
| 插入基因名称: | G3BP1-AG-NS3a(H1) |
| 物种来源: | synthetic |
| 密码子优化: | 哺乳细胞表达优化 |
|
文献引用方法 |
|
| 参考文献部分: |
Programmable solid-state condensates for spatiotemporal control of mammalian gene expression
Wang Y., Jiang J., Xiong Q., Li S., Shao J., Xie M., Zeng A.P.
Nat. Chem. Biol. 2025 1–10 (2025)
|
pYK154
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pYK154载体质粒基本信息
pYK154质粒图谱载体图谱和pYK154载体序列质粒序列多克隆位点信息
pYK154质粒载体简介
pYK154质粒序列载体序列
LOCUS pYK154 8412 bp DNA circular SYN 11-FEB-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8412)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Jul 24, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..8412
/mol_type="other DNA"
/organism="synthetic DNA construct"
misc_feature 232..819
/label=CMV
primer_bind 863..882
/label=T7
primer_bind 881..901
/label=oYK30-NS3a_Head-F
primer_bind 881..901
/label=oYK165_G3BP1_cut_F
primer_bind 881..900
/label=oYK06-ANR-mEGFP-F
promoter 881
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind 914..963
/label=oCW20
primer_bind 926..963
/label=oQZ238
primer_bind 934..963
/label=oCW18
gene 941..2338
/gene="G3BP1"
/gene_synonym="G3BP; HDH-VIII"
/label=G3BP1
/note="G3BP stress granule assembly factor 1"
/db_xref="GeneID:10146"
/db_xref="HGNC:HGNC:30292"
/db_xref="MIM:608431"
misc_feature 941..1366
/label=NTF2L
misc_feature 1367..1618
/label=IDR1
primer_bind 1367..1386
/label=oYK165_G3BP1_cut_F
misc_feature 1367..1369
/gene="G3BP1"
/gene_synonym="G3BP; HDH-VIII"
/note="Phosphothreonine./evidence=ECO:0007744|PubMed:186696
48; propagated from UniProtKB/Swiss-Prot (Q13283.1);
phosphorylation site"
misc_feature 1385..1387
/gene="G3BP1"
/gene_synonym="G3BP; HDH-VIII"
/note="Phosphoserine./evidence=ECO:0000269|PubMed:11604510,
ECO:0007744|PubMed:17081983, ECO:0007744|PubMed:17693683,
ECO:0007744|PubMed:18220336, ECO:0007744|PubMed:18318008,
ECO:0007744|PubMed:18669648, ECO:0007744|PubMed:18691976,
ECO:0007744|PubMed:20068231, ECO:0007744|PubMed:21406692,
ECO:0007744|PubMed:23186163, ECO:0007744|PubMed:24275569;
propagated from UniProtKB/Swiss-Prot (Q13283.1);
phosphorylation site"
misc_feature 1619..1942
/label=IDR2
misc_feature 1631..1633
/gene="G3BP1"
/gene_synonym="G3BP; HDH-VIII"
/note="Phosphoserine.
/evidence=ECO:0000269|Ref.6;propagated from
UniProtKB/Swiss-Prot (Q13283.1); phosphorylation site"
misc_feature 1634..1636
/gene="G3BP1"
/gene_synonym="G3BP; HDH-VIII"
/note="Phosphoserine./evidence=ECO:0000269|PubMed:11604510,
ECO:0000269|Ref.6, ECO:0007744|PubMed:18669648,
ECO:0007744|PubMed:19690332, ECO:0007744|PubMed:20068231,
ECO:0007744|PubMed:21406692, ECO:0007744|PubMed:24275569;
propagated from UniProtKB/Swiss-Prot (Q13283.1);
phosphorylation site"
misc_feature 1688..1690
/gene="G3BP1"
/gene_synonym="G3BP; HDH-VIII"
/note="Phosphoserine./evidence=ECO:0007744|PubMed:23186163;
propagated from UniProtKB/Swiss-Prot (Q13283.1);
phosphorylation site"
misc_feature 1697..1699
/gene="G3BP1"
/gene_synonym="G3BP; HDH-VIII"
/note="Phosphoserine./evidence=ECO:0007744|PubMed:23186163;
propagated from UniProtKB/Swiss-Prot (Q13283.1);
phosphorylation site"
primer_bind 1906..1916
/label=Oscl9-1
/note="for primer of EGFP"
misc_feature 1913..1918
/gene="G3BP1"
/gene_synonym="G3BP; HDH-VIII"
/note="Cleavage, by human enterovirus 71 protease 3C.
/evidence=ECO:0000269|PubMed:30006004; propagated from
UniProtKB/Swiss-Prot (Q13283.1); cleavage site"
misc_feature 1943..2170
/label=RRM
misc_feature 2057..2059
/gene="G3BP1"
/gene_synonym="G3BP; HDH-VIII"
/note="Phosphoserine./evidence=ECO:0007744|PubMed:18669648,
ECO:0007744|PubMed:20068231; propagated from
UniProtKB/Swiss-Prot (Q13283.1); phosphorylation site"
misc_feature 2066..2068
/gene="G3BP1"
/gene_synonym="G3BP; HDH-VIII"
/note="N6-acetyllysine, alternate.
/evidence=ECO:0007744|PubMed:19608861; propagated from
UniProtKB/Swiss-Prot (Q13283.1); acetylation site"
misc_feature 2171..2338
/label=IDR3
misc_feature 2225..2227
/gene="G3BP1"
/gene_synonym="G3BP; HDH-VIII"
/note="Asymmetric dimethylarginine.
/evidence=ECO:0007744|PubMed:24129315; propagated from
UniProtKB/Swiss-Prot (Q13283.1); methylation site"
misc_feature 2243..2245
/gene="G3BP1"
/gene_synonym="G3BP; HDH-VIII"
/note="Asymmetric dimethylarginine, alternate.
/evidence=ECO:0007744|PubMed:24129315; Dimethylated
arginine, alternate. /evidence=ECO:0000269|Ref.6;
Omega-N-methylarginine, alternate.
/evidence=ECO:0000269|Ref.6, ECO:0007744|PubMed:24129315;
propagated from UniProtKB/Swiss-Prot (Q13283.1);
methylation site"
misc_feature 2279..2281
/gene="G3BP1"
/gene_synonym="G3BP; HDH-VIII"
/note="Omega-N-methylarginine.
/evidence=ECO:0007744|PubMed:24129315; propagated from
UniProtKB/Swiss-Prot (Q13283.1); methylation site"
primer_bind complement(2294..2340)
/label=oCW21
misc_feature 2318..2320
/gene="G3BP1"
/gene_synonym="G3BP; HDH-VIII"
/note="Dimethylated arginine, alternate.
/evidence=ECO:0000269|Ref.6; Omega-N-methylarginine,
alternate. /evidence=ECO:0000269|Ref.6,
ECO:0007744|PubMed:24129315; propagated from
UniProtKB/Swiss-Prot (Q13283.1); methylation site"
primer_bind complement(2328..2358)
/label=oYK79_G3BP1_R
misc_feature 2333..2335
/gene="G3BP1"
/gene_synonym="G3BP; HDH-VIII"
/note="Omega-N-methylarginine.
/evidence=ECO:0007744|PubMed:24129315; propagated from
UniProtKB/Swiss-Prot (Q13283.1); methylation site"
misc_feature 2339..2566
/label=Long_Linker
primer_bind 2339..2358
/label=oYK78_AG_ANR1_F
primer_bind 2339..2356
/label=oYK69_AG_ANR1_F
misc_feature 2339..2340
/label=linker
misc_feature 2567..3241
/label=AG
primer_bind 3015..3025
/label=oSCL1104
primer_bind complement(3229..3249)
/label=oYK65-AG-R
misc_feature 3242..3292
/label=Short_Linker
primer_bind complement(3268..3292)
/label=oYK33-35-PB1-AG-R
primer_bind 3273..3313
/label=oYK153_NS3a_F
CDS 3293..3880
/codon_start=1
/label=NS3a H1
/translation="KKKGSVVIVGRINLSGDTAYSQQTRGLEGCQETSQTGRDKNQVEG
EVQVVSTATQSFLATSINGVLWTVYHGAGTRTIASPKGPVTQMYTNVDKDLVGWQAPQG
SRSLTPCTCGSSDLYLVTRHADVIPVRGRGDSRGSLLSPRPISYLKGSSGGPLLCPAGH
AVGIFRAAVSTRGVAKAVDFIPVESLETTMRSP"
primer_bind 3293..3328
/label=oSCL1082
primer_bind 3293..3313
/label=oyk03-03-F
primer_bind 3293..3313
/label=oYK04-ns3a-F
primer_bind 3293..3311
/label=oYK25-NS3a-F
primer_bind 3293..3310
/label=oYK30-NS3a_Head-F
primer_bind 3310..3368
/label=oSCL1083
primer_bind 3349..3407
/label=oSCL1084
primer_bind 3386..3444
/label=oSCL1085
primer_bind 3423..3481
/label=oSCL1086
primer_bind complement(3451..3470)
/label=oSCL1106
primer_bind 3452..3490
/label=oSCL1104
primer_bind complement(3687..3745)
/label=oSCL1105
primer_bind complement(3727..3798)
/label=oSCL1087
primer_bind 3729..3750
/label=oSCL1107
primer_bind 3780..3798
/label=oSCL1088
primer_bind complement(3862..3909)
/label=oSCL1089
primer_bind complement(3865..3912)
/label=oYK30-NS3a_head-R
primer_bind complement(3881..3929)
/label=oYK03-03-R
primer_bind complement(3881..3900)
/label=oSCL1130
primer_bind complement(3889..3909)
/label=olyl382
primer_bind complement(3910..3932)
/label=oYK57-MCP-mCherry-R
primer_bind complement(3910..3929)
/label=oYK25-NS3a-R
primer_bind complement(3934..3953)
/label=oSCL1089
terminator 4006..4233
/label=bGH PA
misc_feature 4279..4707
/label=f1 origin
misc_feature 4712..5055
/label=SV40 early promoter and origin
misc_feature 4901..4978
/label=SV40 ORI
CDS 5120..5911
/label=Kan/neoR
misc_feature 6085..6215
/label=SV40 early polyadenylation signal
primer_bind 6189..6208
/label=olyl382
primer_bind complement(6251..6271)
/label=M13-rev
misc_binding complement(6277..6299)
/label=LacO
promoter complement(6304..6333)
/label=lac
primer_bind 6448..6459
/label=oYK69_AG_ANR1_F
misc_feature complement(6598..7271)
/label=pUC origin
CDS complement(7419..8078)
/label=AmpR
primer_bind 7735..7746
/label=oyk03-03-F
primer_bind 7735..7746
/label=oYK04-ns3a-F
primer_bind 7735..7746
/label=oYK153_NS3a_F
promoter complement(8318..8346)
/label=Amp prom
ORIGIN
1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
901 gtttaaactt aagcttggta ccgagctcgg atccgccacc atggtgatgg agaagcctag
961 tcccctgctg gtcgggcggg aatttgtgag acagtattac acactgctga accaggcccc
1021 agacatgctg catagatttt atggaaagaa ctcttcttat gtccatgggg gattggattc
1081 aaatggaaag ccagcagatg cagtctacgg acagaaagaa atccacagga aagtgatgtc
1141 acaaaacttc accaactgcc acaccaagat tcgccatgtt gatgctcatg ccacgctaaa
1201 tgatggtgtg gtagtccagg tgatggggct tctctctaac aacaaccagg ctttgaggag
1261 attcatgcaa acgtttgtcc ttgctcctga ggggtctgtt gcaaataaat tctatgttca
1321 caatgatatc ttcagatacc aagatgaggt ctttggtggg tttgtcactg agcctcagga
1381 ggagtctgaa gaagaagtag aggaacctga agaaagacag caaacacctg aggtggtacc
1441 tgatgattct ggaactttct atgatcaggc agttgtcagt aatgacatgg aagaacattt
1501 agaggagcct gttgctgaac cagagcctga tcctgaacca gaaccagaac aagaacctgt
1561 atctgaaatc caagaggaaa agcctgagcc agtattagaa gaaactgccc ctgaggatgc
1621 tcagaagagt tcttctccag cacctgcaga catagctcag acagtacagg aagacttgag
1681 gacattttct tgggcatctg tgaccagtaa gaatcttcca cccagtggag ctgttccagt
1741 tactgggata ccacctcatg ttgttaaagt accagcttca cagccccgtc cagagtctaa
1801 gcctgaatct cagattccac cacaaagacc tcagcgggat caaagagtgc gagaacaacg
1861 aataaatatt cctccccaaa ggggacccag accaatccgt gaggctggtg agcaaggtga
1921 cattgaaccc cgaagaatgg tgagacaccc tgacagtcac caactcttca ttggcaacct
1981 gcctcatgaa gtggacaaat cagagcttaa agatttcttt caaagttatg gaaacgtggt
2041 ggagttgcgc attaacagtg gtgggaaatt acccaatttt ggttttgttg tgtttgatga
2101 ttctgagcct gttcagaaag tccttagcaa caggcccatc atgttcagag gtgaggtccg
2161 tctgaatgtc gaagagaaga agactcgagc tgccagggaa ggcgaccgac gagataatcg
2221 ccttcgggga cctggaggcc ctcgaggtgg gctgggtggt ggaatgagag gccctccccg
2281 tggaggcatg gtgcagaaac caggatttgg agtgggaagg gggcttgcgc cacggcaggg
2341 ttctgggagt ggcggtagtg gtgctggagg cagcgcaggc tctggagcag gcggcagtgc
2401 cggaagtggc gctggagggt ctgccggatc tggagccggt ggttctgccg gctccggcgc
2461 tggtgggagc gcttccggaa gtagtcccgg taattccgct gacggcggcg gaggatcggg
2521 tggtagtggt ggttcaggag gaggatcgac ccaaggagga tccatggtga gcgtgatcaa
2581 gcccgagatg aagatcaagc tgtgcatgag gggcaccgtg aacggccaca acttcgtgat
2641 cgagggcgag ggcaagggca acccctacga gggcacccag atcctggacc tgaacgtgac
2701 cgagggcgcc cccctgccct tcgcctacga catcctgacc accgtgttcc agtacggcaa
2761 cagggccttc accaagtacc ccgccgacat ccaggactac ttcaagcaga ccttccccga
2821 gggctaccac tgggagagga gcatgaccta cgaggaccag ggcatctgca ccgccaccag
2881 caacatcagc atgaggggcg actgcttctt ctacgacatc aggttcgacg gcgtgaactt
2941 cccccccaac ggccccgtga tgcagaagaa gaccctgaag tgggagccca gcaccgagaa
3001 gatgtacgtg agggacggcg tgctgaaggg cgacgtgaac atggccctgc tgctggaggg
3061 cggcggccac tacaggtgcg acttcaagac cacctacaag gccaagaagg acgtgaggct
3121 gcccgactac cacttcgtgg accacaggat cgagatcctg aagcacgaca aggactacaa
3181 caaggtgaag ctgtacgaga acgccgtggc caggtacagc atgctgccca gccaggccaa
3241 gggtaccgga actgcagcag agaattcggg aaactcgaga acaaagcttg aaaagaagaa
3301 gggcagcgtg gtgatcgtgg gcagaatcaa cctgagcggc gacaccgcct acagccagca
3361 gaccagaggc ctggagggct gccaggagac cagccagacc ggcagagaca agaaccaggt
3421 ggagggcgag gtgcaggtgg tgagcaccgc cacccagagc ttcctggcca ccagcatcaa
3481 cggcgtgctg tggaccgtgt accacggcgc cggcaccaga accatcgcca gccccaaggg
3541 ccccgtgacc cagatgtaca cgaacgtgga caaggactta gtgggctggc aggcccccca
3601 aggcagcaga agcctgaccc cctgcacctg cggcagcagc gacctgtacc tggtgaccag
3661 acacgccgac gtgatccccg tgagaggaag aggcgacagc agaggcagcc tgctgagccc
3721 cagacccatc agctatctga aaggctcaag cggcggcccc ctgttgtgtc ccgccggcca
3781 tgccgtgggc atcttcagag ccgccgtgag caccagaggc gtggccaagg ccgtggactt
3841 catccccgtg gagagcctgg agaccaccat gagaagcccc gctagctaga aattctgcag
3901 atatccagcg gatccactag tccagtgtgg tggaattctg cagatatcca gcacagtggc
3961 ggccgctcga gtctagaggg cccgtttaaa cccgctgatc agcctcgact gtgccttcta
4021 gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca
4081 ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc
4141 attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagacaata
4201 gcaggcatgc tggggatgcg gtgggctcta tggcttctga ggcggaaaga accagctggg
4261 gctctagggg gtatccccac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg
4321 ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct
4381 tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc
4441 ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg
4501 atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt
4561 ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg
4621 tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc
4681 tgatttaaca aaaatttaac gcgaattaat tctgtggaat gtgtgtcagt tagggtgtgg
4741 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
4801 aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
4861 caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc
4921 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga
4981 ggccgcctct gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg
5041 cttttgcaaa aagctcccgg gagcttgtat atccattttc ggatctgatc aagagacagg
5101 atgaggatcg tttcgcatga ttgaacaaga tggattgcac gcaggttctc cggccgcttg
5161 ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct ctgatgccgc
5221 cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg acctgtccgg
5281 tgccctgaat gaactgcagg acgaggcagc gcggctatcg tggctggcca cgacgggcgt
5341 tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc tgctattggg
5401 cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga aagtatccat
5461 catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc cattcgacca
5521 ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc ttgtcgatca
5581 ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg ccaggctcaa
5641 ggcgcgcatg cccgacggcg aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa
5701 tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc tgggtgtggc
5761 ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc ttggcggcga
5821 atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc agcgcatcgc
5881 cttctatcgc cttcttgacg agttcttctg agcgggactc tggggttcga aatgaccgac
5941 caagcgacgc ccaacctgcc atcacgagat ttcgattcca ccgccgcctt ctatgaaagg
6001 ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg cggggatctc
6061 atgctggagt tcttcgccca ccccaacttg tttattgcag cttataatgg ttacaaataa
6121 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
6181 ttgtccaaac tcatcaatgt atcttatcat gtctgtatac cgtcgacctc tagctagagc
6241 ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca
6301 cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa
6361 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
6421 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
6481 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
6541 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
6601 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
6661 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
6721 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
6781 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
6841 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
6901 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
6961 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
7021 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
7081 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
7141 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
7201 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
7261 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
7321 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
7381 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
7441 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
7501 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
7561 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
7621 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
7681 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
7741 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
7801 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
7861 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
7921 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
7981 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
8041 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
8101 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
8161 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
8221 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
8281 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
8341 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
8401 ccacctgacg tc
//
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