| 质粒名称: | pAAVLINK-EF1a-RELN-A |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001735 |
| 产品用途: | 5' AAVLINK plasmid for RELN expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒 |
| 启动子: | EF1a |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | RELN |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
pAAVLINK-EF1a-RELN-A
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAVLINK-EF1a-RELN-A载体质粒基本信息
pAAVLINK-EF1a-RELN-A质粒图谱载体图谱和pAAVLINK-EF1a-RELN-A载体序列质粒序列多克隆位点信息
pAAVLINK-EF1a-RELN-A质粒载体简介
pAAVLINK-EF1a-RELN-A质粒序列载体序列
LOCUS pAAVLINK-EF1a-RELN-A 7176 bp DNA circular SYN 21-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7176)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported Jun 4, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..7176
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
promoter 146..688
/label=EF1-alpha
/note="Chimeric promoter of ef1a and htlv 5' UTR"
promoter 175..386
/label=EF-1-alpha core promoter
/note="core promoter for human elongation factor
EF-1-alpha"
LTR 399..667
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from human
T-cell leukemia virus (HTLV) type 1"
misc_feature 493..498
/label=SDS
regulatory 695..704
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
CDS 701..4316
/codon_start=1
/label=RELN
/translation="MERSGWARQTFLLALLLGATLRARAAAGYYPRFSPFFFLCTHHGE
LEGDGEQGEVLISLHIAGNPTYYVPGQEYHVTISTSTFFDGLLVTGLYTSTSVQASQSI
GGSSAFGFGIMSDHQFGNQFMCSVVASHVSHLPTTNLSFIWIAPPAGTGCVNFMATATH
RGQVIFKDALAQQLCEQGAPTDVTVHPHLAEIHSDSIILRDDFDSYHQLQLNPNIWVEC
NNCETGEQCGAIMHGNAVTFCEPYGPRELITTGLNTTTASVLQFSIGSGSCRFSYSDPS
IIVLYAKNNSADWIQLEKIRAPSNVSTIIHILYLPEDAKGENVQFQWKQENLRVGEVYE
ACWALDNILIINSAHRQVVLEDSLDPVDTGNWLFFPGATVKHSCQSDGNSIYFHGNEGS
EFNFATTRDVDLSTEDIQEQWSEEFESQPTGWDVLGAVIGTECGTIESGLSMVFLKDGE
RKLCTPSMDTTGYGNLRFYFVMGGICDPGNSHENDIILYAKIEGRKEHITLDTLSYSSY
KVPSLVSVVINPELQTPATKFCLRQKNHQGHNRNVWAVDFFHVLPVLPSTMSHMIQFSI
NLGCGTHQPGNSVSLEFSTNHGRSWSLLHTECLPEICAGPHLPHSTVYSSENYSGWNRI
TIPLPNAALTRNTRIRWRQTGPILGNMWAIDNVYIGPSCLKFCSGRGQCTRHGCKCDPG
FSGPACEMASQTFPMFISESFGSSRLSSYHNFYSIRGAEVSFGCGVLASGKALVFNKDG
RRQLITSFLDSSQSRFLQFTLRLGSKSVLSTCRAPDQPGEGVLLHYSYDNGITWKLLEH
YSYLSYHEPRIISVELPGDAKQFGIQFRWWQPYHSSQREDVWAIDEIIMTSVLFNSISL
DFTNLVEVTQSLGFYLGNVQPYCGHDWTLCFTGDSKLASSMRYVETQSMQIGASYMIQF
SLVMGCGQKYTPHMDNQVKLEYSTNHGLTWHLVQEECLPSMPSCQEFTSASIYHASEFT
QWRRVIVLLPQKTWSSATRFRWSQSYYTAQDEWALDSIYIGQQCPNMCSGHGSCDHGIC
RCDQGYQGTECHPEAALPSTIMSDFENQNGWESDWQEVIGGEIVKPEQGCGVISSGSSL
YFSKAGKRQLVSWDLDTSWVDFVQFYIQIGGESASCNKPDSREEGVLLQYSNNGGIQWH
LLAEMYFSDFSKPRFVYLELPAAAKTPCTRFRWWQPVFS"
misc_feature 733..738
/label=SDS
CDS 2198..2209
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
misc_feature 2506..2511
/label=SDS
misc_feature 4149..4154
/label=SDS
misc_feature 4159..4164
/label=SDS
intron 4317..4357
/label=Splicing Donor
misc_feature 4361..4394
/label=loxP-JT1571
repeat_region 4439..4579
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 4439..4568
/label=AAV2 ITR
rep_origin 4654..5109
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 5391..5495
/gene="bla"
/label=AmpR promoter
CDS 5496..6356
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 6527..7115
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtggatct gcgatcgctc cggtgcccgt cagtgggcag
181 agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat tgaacgggtg
241 cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt
301 ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac gttctttttc
361 gcaacgggtt tgccgccaga acacagctga agcttcgagg ggctcgcatc tctccttcac
421 gcgcccgccg ccctacctga ggccgccatc cacgccggtt gagtcgcgtt ctgccgcctc
481 ccgcctgtgg tgcctcctga actgcgtccg ccgtctaggt aagtttaaag ctcaggtcga
541 gaccgggcct ttgtccggcg ctcccttgga gcctacctag actcagccgg ctctccacgc
601 tttgcctgac cctgcttgct caactctacg tctttgtttc gttttctgtt ctgcgccgtt
661 acagatccaa gctgtgaccg gcgcctaccg tacggccacc atggagcgca gtggctgggc
721 ccggcagact ttcctcctag cgctgttgct gggggcgacg ctgagggcgc gcgcggcggc
781 tggctattac ccccgctttt cgcccttctt tttcctgtgc acccaccacg gggagctgga
841 aggggatggg gagcagggcg aggtgctcat ttccctgcat attgcgggca accccaccta
901 ctacgttccg ggacaagaat accatgtgac aatttcaaca agcacctttt ttgacggctt
961 gctggtgaca ggactataca catctacaag tgttcaggca tcacagagca ttggaggttc
1021 cagtgctttc ggatttggga tcatgtctga ccaccagttt ggtaaccagt ttatgtgcag
1081 tgtggtagcc tctcacgtga gtcacctgcc cacaaccaac ctcagtttca tctggattgc
1141 tccacctgcg ggcacaggct gtgtgaattt catggctaca gcaacacacc ggggccaggt
1201 tattttcaaa gatgctttag cccagcagtt gtgtgaacaa ggagctccaa cagatgtcac
1261 tgtgcaccca catctagctg aaatacatag tgacagcatt atcctgagag atgactttga
1321 ctcctaccac caactgcaat taaatccaaa tatatgggtt gaatgtaaca actgtgagac
1381 tggagaacag tgtggcgcga ttatgcatgg caatgccgtc accttctgtg aaccatatgg
1441 cccacgagaa ctgattacca caggccttaa tacaacaaca gcttctgtcc tccaattttc
1501 cattgggtca ggttcatgtc gctttagtta ttcagacccc agcatcatcg tgttatatgc
1561 caagaataac tctgcggact ggattcagct agagaaaatt agagcccctt ccaatgtcag
1621 cacaatcatc catatcctct accttcctga ggacgccaaa ggggagaatg tccaatttca
1681 gtggaagcag gaaaatcttc gtgtaggtga agtgtatgaa gcctgctggg ccttagataa
1741 catcttgatc atcaattcag ctcacagaca agtcgtttta gaagatagtc tcgacccagt
1801 ggacacaggc aactggcttt tcttcccagg agctacagtt aagcatagct gtcagtcaga
1861 tgggaactcc atttatttcc atggaaatga aggcagcgag ttcaattttg ccaccaccag
1921 ggatgtagat ctttccacag aagatattca agagcaatgg tcagaagaat ttgagagcca
1981 gcctacagga tgggatgtct tgggagctgt cattggtaca gaatgtggaa cgatagaatc
2041 aggcttatca atggtcttcc tcaaagatgg agagaggaaa ttatgcactc catccatgga
2101 cactaccggt tatgggaacc tgaggtttta ctttgtgatg ggaggaattt gtgaccctgg
2161 aaattctcat gaaaatgaca taatcctgta tgcaaaaatt gaaggaagaa aagagcatat
2221 aacactggat accctttcct attcctcata taaggttccg tctttggttt ctgtggtcat
2281 caatcctgaa cttcagactc ctgctaccaa attttgtctc aggcaaaaga accatcaagg
2341 acataatagg aatgtctggg ctgtagactt tttccatgtc ttgcctgttc tcccttctac
2401 aatgtctcac atgatacagt tttccatcaa tctgggatgt ggaacgcatc agcctggtaa
2461 cagtgtcagc ttggaatttt ctaccaacca tgggcgctcc tggtccctcc ttcacactga
2521 atgcttacct gagatctgtg ctggacccca cctcccccac agcactgtct actcctctga
2581 aaactacagt gggtggaacc gaataacaat tccccttcct aacgcagcac taacccggaa
2641 caccaggatt cgctggagac aaacaggacc aatccttgga aacatgtggg caattgataa
2701 tgtttatatt ggcccgtcat gtctcaaatt ctgttctggc agaggacagt gcactagaca
2761 tggttgcaag tgtgaccctg gattttctgg cccagcttgt gagatggcat cccagacatt
2821 cccaatgttt atttctgaaa gctttggcag ttccaggctc tcctcttacc ataactttta
2881 ctctatccgt ggtgctgaag tcagctttgg ttgtggtgtc ttggccagtg gtaaggccct
2941 ggttttcaac aaagatgggc ggcgtcagct aattacatct ttccttgaca gctcacaatc
3001 caggtttctc cagttcacac tgagactggg gagcaaatct gttctgagca cgtgcagagc
3061 ccctgatcag cctggtgaag gagttttgtt gcattattct tatgataatg ggataacttg
3121 gaaactcctg gagcattatt catatctcag ctatcatgag cccagaataa tctccgtaga
3181 actaccaggt gatgcaaagc agtttggaat tcagttcaga tggtggcaac cgtatcattc
3241 ttcccagaga gaagatgtat gggctattga tgagattatc atgacatctg tgcttttcaa
3301 cagcattagt cttgacttta ccaatcttgt ggaggtcact cagtctctgg gattctacct
3361 tggaaatgtt cagccatact gtggccacga ctggaccctt tgttttacag gagattctaa
3421 acttgcctca agtatgcgct atgtggaaac acaatcaatg cagataggag catcctatat
3481 gattcagttc agtttggtga tgggatgtgg ccagaaatac accccacaca tggacaacca
3541 ggtgaagctg gagtactcaa ccaaccacgg ccttacctgg cacctcgtcc aagaagaatg
3601 ccttccaagt atgccaagtt gtcaggaatt tacatcagca agtatttacc atgccagtga
3661 gtttacacag tggaggagag tcatagtgct tcttccccag aaaacttggt ccagtgctac
3721 ccgtttccgc tggagccaga gctattacac agctcaagac gagtgggctt tggacagcat
3781 ttacattggg cagcagtgcc ccaacatgtg cagtgggcat ggctcatgcg atcatggcat
3841 atgcaggtgt gaccaggggt accaaggcac tgaatgccac ccagaagctg cccttccgtc
3901 cacaattatg tcagattttg agaaccagaa tggctgggag tctgactggc aagaagttat
3961 tgggggagaa attgtaaaac cagaacaagg gtgtggtgtc atctcttctg gatcatctct
4021 gtacttcagc aaggctggga aaagacagct ggtgagttgg gacctggata cttcttgggt
4081 ggactttgtc cagttctaca tccagatagg cggagagagt gcttcatgca acaagcctga
4141 cagcagagag gagggcgtcc tccttcagta cagcaacaat gggggcatcc agtggcacct
4201 gctagcagag atgtactttt cagacttcag caaacccaga tttgtctatc tggagcttcc
4261 agctgctgcc aagacccctt gcaccaggtt ccgctggtgg cagcccgtgt tctcaggtaa
4321 gtatcaaggt tacaagacag gtttaaggag accaataagc tattattcgt atagcataca
4381 ttatacgaag ttattaagtt atggagaccg gtgtggcacg tgcggaccga gcggccgcag
4441 gaacccctag tgatggagtt ggccactccc tctctgcgcg ctcgctcgct cactgaggcc
4501 gggcgaccaa aggtcgcccg acgcccgggc tttgcccggg cggcctcagt gagcgagcga
4561 gcgcgcagct gcctgcaggg gcgcctgatg cggtattttc tccttacgca tctgtgcggt
4621 atttcacacc gcatacgtca aagcaaccat agtacgcgcc ctgtagcggc gcattaagcg
4681 cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ttagcgcccg
4741 ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc
4801 taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa
4861 aacttgattt gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc
4921 ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac
4981 tcaactctat ctcgggctat tcttttgatt tataagggat tttgccgatt tcggtctatt
5041 ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgt
5101 ttacaatttt atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagc
5161 cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg
5221 cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat
5281 caccgaaacg cgcgagacga aagggcctcg tgatacgcct atttttatag gttaatgtca
5341 tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc
5401 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
5461 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
5521 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
5581 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
5641 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
5701 cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac
5761 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
5821 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
5881 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
5941 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
6001 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc
6061 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
6121 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
6181 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
6241 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
6301 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
6361 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
6421 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
6481 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
6541 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
6601 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
6661 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
6721 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
6781 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
6841 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
6901 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
6961 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
7021 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
7081 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
7141 ggttcctggc cttttgctgg ccttttgctc acatgt
//
