| 质粒名称: | mCherry-Rab5a-7 |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 55126 |
| 存储实验室: | Michael Davidson |
|
载体骨架基本信息 |
|
| 载体名称: | mCherry |
| 载体出品公司: | Clontech |
| 空载体大小: | 4750bp |
| 完整质粒大小: | 5335bp |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | 新霉素（G418） |
| 载体抗性: | Kanamycin, 50 μg/mL |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | Rab5a\NM_004162.4 |
| 启动子: | CMV |
| 克隆方法: | 未知 |
| 5’测序引物 | CMV-F |
| 3’测序引物 | SV40pA-R |
|
文献引用方法 |
|
| 材料和方法部分: | mCherry-Rab5a-7 was a gift from Michael Davidson (Addgene plasmid # 55126 ; http://n2t.net/addgene:55126 ; RRID:Addgene_55126) |
买家导航
mCherry-Rab5a-7载体质粒基本信息
mCherry-Rab5a-7质粒图谱载体图谱和mCherry-Rab5a-7载体序列质粒序列多克隆位点信息
mCherry-Rab5a-7质粒载体简介
mCherry-Rab5a-7质粒序列载体序列
LOCUS mCherry-Rab5a-7 5331 bp DNA circular SYN 14-APR-2025
DEFINITION Localization: Ras GTPase, Excitation: 587, Emission: 610.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5331)
TITLE Davidson lab - mCherry constructs
REFERENCE 2 (bases 1 to 5331)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Apr 14, 2025 from SnapGene Server 8.0.1
https://www.biofeng.com
COMMENT SGRef: number: 1; type: "Journal Article"
COMMENT Sequence Label: mCherry-Rab5a-7
FEATURES Location/Qualifiers
source 1..5331
/mol_type="other DNA"
/organism="synthetic DNA construct"
enhancer 16..319
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 320..523
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 474..494
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
regulatory 562..571
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
CDS 568..1275
/codon_start=1
/product="monomeric derivative of DsRed fluorescent protein
(Shaner et al., 2004)"
/label=mCherry
/note="mammalian codon-optimized"
/translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
EGRHSTGGMDELYK"
primer_bind complement(713..731)
/label=mCherry-R
/note="mCherry, reverse primer"
primer_bind 981..1000
/label=mCherry-F
/note="mCherry, forward primer"
polyA_signal 2074..2195
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(2111..2130)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 2165..2184
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
rep_origin complement(2202..2657)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(2339..2360)
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind 2551..2570
/label=F1ori-R
/note="F1 origin, reverse primer"
promoter 2684..2788
/gene="bla"
/label=AmpR promoter
promoter 2790..3147
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
enhancer 2790..2981
/label=SV40 enhancer
/note="enhancer for the SV40 early promoter (Herr, 1993)"
primer_bind complement(2813..2833)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
rep_origin 2998..3133
/label=SV40 ori
/note="SV40 origin of replication"
CDS 3182..3976
/codon_start=1
/product="aminoglycoside phosphotransferase"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
CDS 3182..3976
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(3236..3255)
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
primer_bind 3846..3865
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
primer_bind complement(4164..4183)
/label=TK-pA-R
/note="Thymidine kinase polyA, reverse primer"
polyA_signal 4208..4255
/label=HSV TK poly(A) signal
/note="herpes simplex virus thymidine kinase
polyadenylation signal (Cole and Stacy, 1985)"
rep_origin 4584..5172
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 5073..5092
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
ORIGIN
1 atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa
61 cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac
121 tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca
181 agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg
241 gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt
301 agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg
361 gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg
421 gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat
481 gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctggtttag tgaaccgtca
541 gatccgctag cgctaccggt cgccaccatg gtgagcaagg gcgaggagga taacatggcc
601 atcatcaagg agttcatgcg cttcaaggtg cacatggagg gctccgtgaa cggccacgag
661 ttcgagatcg agggcgaggg cgagggccgc ccctacgagg gcacccagac cgccaagctg
721 aaggtgacca agggtggccc cctgcccttc gcctgggaca tcctgtcccc tcagttcatg
781 tacggctcca aggcctacgt gaagcacccc gccgacatcc ccgactactt gaagctgtcc
841 ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg cgtggtgacc
901 gtgacccagg actcctccct gcaggacggc gagttcatct acaaggtgaa gctgcgcggc
961 accaacttcc cctccgacgg ccccgtaatg cagaagaaga ccatgggctg ggaggcctcc
1021 tccgagcgga tgtaccccga ggacggcgcc ctgaagggcg agatcaagca gaggctgaag
1081 ctgaaggacg gcggccacta cgacgctgag gtcaagacca cctacaaggc caagaagccc
1141 gtgcagctgc ccggcgccta caacgtcaac atcaagttgg acatcacctc ccacaacgag
1201 gactacacca tcgtggaaca gtacgaacgc gccgagggcc gccactccac cggcggcatg
1261 gacgagctgt acaagtccgg actcagatct cgagccatgg ctagtcgagg cgcaacaaga
1321 cccaacgggc caaatactgg aaataaaata tgccagttca aactagtact tctgggagag
1381 tctgctgttg gcaaatcaag cctagtgctt cgttttgtga agggccaatt tcatgaattt
1441 caagagagta ccataggggc tgcttttcta acccaaactg tgtgtcttga tgatacaaca
1501 gtaaagtttg aaatatggga tacagctggt caagaacgat accatagctt agcaccaatg
1561 tactacagag gagcacaagc agccatagtt gtatatgata tcacaaatga ggagtccttt
1621 gccagagcca aaaactgggt taaagaactt cagaggcaag ccagtcctaa cattgtaata
1681 gctttatcag gaaacaaggc tgatcttgca aataaaagag ctgtcgattt ccaggaagca
1741 cagtcctatg cagatgacaa cagtttatta ttcatggaga catcagctaa aacatcgatg
1801 aatgtaaatg aaatattcat ggcaatagct aaaaagttgc caaagaacga accacagaat
1861 ccaggagcaa attctgccag aggaagagga gtagacctta ctgaacccac gcagccaacc
1921 aggaatcagt gttgtagtaa ctaaggatcc accggatcta gataactgat cataatcagc
1981 cataccacat ttgtagaggt tttacttgct ttaaaaaacc tcccacacct ccccctgaac
2041 ctgaaacata aaatgaatgc aattgttgtt gttaacttgt ttattgcagc ttataatggt
2101 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
2161 agttgtggtt tgtccaaact catcaatgta tcttaacgcg taaattgtaa gcgttaatat
2221 tttgttaaaa ttcgcgttaa atttttgtta aatcagctca ttttttaacc aataggccga
2281 aatcggcaaa atcccttata aatcaaaaga atagaccgag atagggttga gtgttgttcc
2341 agtttggaac aagagtccac tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac
2401 cgtctatcag ggcgatggcc cactacgtga accatcaccc taatcaagtt ttttggggtc
2461 gaggtgccgt aaagcactaa atcggaaccc taaagggagc ccccgattta gagcttgacg
2521 gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag cgggcgctag
2581 ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc
2641 gccgctacag ggcgcgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
2701 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
2761 gcttcaataa tattgaaaaa ggaagagtcc tgaggcggaa agaaccagct gtggaatgtg
2821 tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
2881 catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt
2941 atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc
3001 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt
3061 atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc
3121 ttttttggag gcctaggctt ttgcaaagat cgatcaagag acaggatgag gatcgtttcg
3181 catgattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt
3241 cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc
3301 agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact
3361 gcaagacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt
3421 gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca
3481 ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat
3541 gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg
3601 catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga
3661 agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcga gcatgcccga
3721 cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa
3781 tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga
3841 catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt
3901 cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct
3961 tgacgagttc ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc gacgcccaac
4021 ctgccatcac gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc
4081 gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc
4141 gcccacccta gggggaggct aactgaaaca cggaaggaga caataccgga aggaacccgc
4201 gctatgacgg caataaaaag acagaataaa acgcacggtg ttgggtcgtt tgttcataaa
4261 cgcggggttc ggtcccaggg ctggcactct gtcgataccc caccgagacc ccattggggc
4321 caatacgccc gcgtttcttc cttttcccca ccccaccccc caagttcggg tgaaggccca
4381 gggctcgcag ccaacgtcgg ggcggcaggc cctgccatag cctcaggtta ctcatatata
4441 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
4501 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
4561 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
4621 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
4681 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
4741 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
4801 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
4861 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
4921 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
4981 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
5041 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
5101 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
5161 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
5221 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
5281 atgcattagt tattaatagt aatcaattac ggggtcatta gttcatagcc c
//
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