| 质粒名称: | HP4_M13 |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 120340 |
| 存储实验室: | Hendrik Dietz |
|
载体骨架基本信息 |
|
| 载体名称: | psB4K5 |
| 载体出品公司: | BioBrick registry |
| 空载体大小: | 3200bp |
| 完整质粒大小: | 9448bp |
| 载体类型: | 细菌表达 |
| 载体抗性: | Kanamycin |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 低拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | Coding part of the M13 phage genome |
| 物种来源: | phage M13 |
| 插入大小: | 6300bp |
| 启动子: | native phage promoters |
| 克隆方法: | 未知 |
| 5’测序引物 | tgccacctgacgtctaagaa |
| 3’测序引物 | attaccgcctttgagtgagc |
|
文献引用方法 |
|
| 材料和方法部分: | HP4_M13 was a gift from Hendrik Dietz (Addgene plasmid # 120340 ; http://n2t.net/addgene:120340 ; RRID:Addgene_120340) |
| 参考文献部分: | Biotechnological mass production of DNA origami. Praetorius F, Kick B, Behler KL, Honemann MN, Weuster-Botz D, Dietz H. Nature. 2017 Dec 6;552(7683):84-87. doi: 10.1038/nature24650. 10.1038/nature24650 PubMed 29219963 |
买家导航
HP4_M13载体质粒基本信息
HP4_M13质粒图谱载体图谱和HP4_M13载体序列质粒序列多克隆位点信息
HP4_M13质粒载体简介
HP4_M13质粒序列载体序列
LOCUS HP4_M13 9447 bp DNA circular SYN 14-APR-2025
DEFINITION Helper plasmid for production of phagemid ssDNA, based on m13mp18.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9447)
AUTHORS Praetorius F, Kick B, Behler KL, Honemann MN, Weuster-Botz D, Dietz
H
TITLE Biotechnological mass production of DNA origami.
JOURNAL Nature. 2017 Dec 6;552(7683):84-87. doi: 10.1038/nature24650.
PUBMED 29219963
REFERENCE 2 (bases 1 to 9447)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Apr 14, 2025 from SnapGene Server 8.0.1
https://www.biofeng.com
COMMENT SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName:
"Nature"; date: "2017-12-6- 6"; volume: "552"; issue: "7683"; pages:
"84-87"
COMMENT Sequence Label: HP4_M13
FEATURES Location/Qualifiers
source 1..9447
/mol_type="other DNA"
/organism="synthetic DNA construct"
terminator complement(83..112)
/label=T3Te terminator
/note="phage T3 early transcription terminator"
CDS 323..1555
/codon_start=1
/gene="II"
/product="pII"
/label=M13 gene II
/note="replication"
/translation="MIDMLVLRLPFIDSLVCSRLSGNDLIAFVDLSKIATLSGINLSAR
TVEYHIDGDLTVSGLSHPFESLPTHYSGIAFKIYEGSKNFYPCVEIKASPAKVLQGHNV
FGTTDLALCSEALLLNFANSLPCLYDLLDVNATTISRIDATFSARAPNENIAKQVIDHL
RNVSNGQTKSTRSQNWESTVIWNETSRHRTLVAYLKHVELQHYIQQLSSKPSAKMTSYQ
KEQLKVLSNPDLLEFASGLVRFEARIKTRYLKSFGLPLNLFDAIRFASDYNSQGKDLIF
DLWSFSFSELFKAFEGDSMNIYDDSAVLDAIQSKHFTITPSGKTSFAKASRYFGFYRRL
VNEGYDSVALTMPRNSFWRYVSALVECGIPKSQLMNLSTCNNVVPLVRFINVDFSSQRP
DWYNEPVLKIA"
CDS 1567..1830
/codon_start=1
/gene="V"
/product="pV"
/label=M13 gene V
/note="replication"
/translation="MIKVEIKPSQAQFTTRSGVSRQGKPYSLNEQLCYVDLGNEYPVLV
KITLDEGQPAYAPGLYTVHLSSFKVGQFGSLMIDRLRLVPAK"
CDS 1832..1933
/codon_start=1
/gene="VII"
/product="pVII"
/label=M13 gene VII
/note="minor coat protein"
/translation="MEQVADFDTIYQAMIQISVVLCFALGIIAGGQR"
CDS 1930..2028
/codon_start=1
/gene="IX"
/product="pIX"
/label=M13 gene IX
/note="minor coat protein"
/translation="MSVLVYSFASFVLGWCLRSGITYFTRLMETSS"
CDS 2025..2246
/codon_start=1
/gene="VIII"
/product="pVIII"
/label=M13 gene VIII
/note="major coat protein"
/translation="MKKSLVLKASVAVATLVPMLSFAAEGDDPAKAAFNSLQASATEYI
GYAWAMVVVIVGATIGIKLFKKFTSKAS"
terminator 2250..2297
/label=fd terminator
/note="central terminator from bacteriophage fd (Otsuka and
Kunisawa, 1982)"
CDS 2302..3576
/codon_start=1
/gene="III"
/product="pIII"
/label=M13 gene III
/note="minor coat protein"
/translation="MKKLLFAIPLVVPFYSHSAETVESCLAKSHTENSFTNVWKDDKTL
DRYANYEGCLWNATGVVVCTGDETQCYGTWVPIGLAIPENEGGGSEGGGSEGGGSEGGG
TKPPEYGDTPIPGYTYINPLDGTYPPGTEQNPANPNPSLEESQPLNTFMFQNNRFRNRQ
GALTVYTGTVTQGTDPVKTYYQYTPVSSKAMYDAYWNGKFRDCAFHSGFNEDLFVCEYQ
GQSSDLPQPPVNAGGGSGGGSGGGSEGGGSEGGGSEGGGSEGGGSGGGSGSGDFDYEKM
ANANKGAMTENADENALQSDAKGKLDSVATDYGAAIDGFIGDVSGLANGNGATGDFAGS
NSQMAQVGDGDNSPLMNNFRQYLPSLPQSVECRPFVFGAGKPYEFSIDCDKINLFRGVF
AFLLYVATFMYVFSTFANILRNKES"
CDS 3579..3917
/codon_start=1
/gene="VI"
/product="pVI"
/label=M13 gene VI
/note="minor coat protein"
/translation="MPVLLGIPLLLRFLGFLLVTLFGYLLTFLKKGFGKIAIAISLFLA
LIIGLNSILVGYLSDISAQLPSDFVQGVQLILPSNALPCFYVILSVKAAIFIFDVKQKI
VSYLDWDK"
CDS 3919..4965
/codon_start=1
/gene="I"
/product="pI"
/label=M13 gene I
/note="phage assembly"
/translation="MAVYFVTGKLGSGKTLVSVGKIQDKIVAGCKIATNLDLRLQNLPQ
VGRFAKTPRVLRIPDKPSISDLLAIGRGNDSYDENKNGLLVLDECGTWFNTRSWNDKER
QPIIDWFLHARKLGWDIIFLVQDLSIVDKQARSALAEHVVYCRRLDRITLPFVGTLYSL
ITGSKMPLPKLHVGVVKYGDSQLSPTVERWLYTGKNLYNAYDTKQAFSSNYDSGVYSYL
TPYLSHGRYFKPLNLGQKMKLTKIYLKKFSRVLCLAIGFASAFTYSYITQPKPEVKKVV
SQTYDFDKFTIDSSQRLNLSYRYVFKDSKGKLINSDDLQKQGYSLTYIDLCTVSIKKGN
SNEIVKCN"
CDS 4943..6223
/codon_start=1
/gene="IV"
/product="pIV"
/label=M13 gene IV
/note="phage assembly"
/translation="MKLLNVINFVFLMFVSSSSFAQVIEMNNSPLRDFVTWYSKQSGES
VIVSPDVKGTVTVYSSDVKPENLRNFFISVLRANNFDMVGSNPSIIQKYNPNNQDYIDE
LPSSDNQEYDDNSAPSGGFFVPQNDNVTQTFKINNVRAKDLIRVVELFVKSNTSKSSNV
LSIDGSNLLVVSAPKDILDNLPQFLSTVDLPTDQILIEGLIFEVQQGDALDFSFAAGSQ
RGTVAGGVNTDRLTSVLSSAGGSFGIFNGDVLGLSVRALKTNSHSKILSVPRILTLSGQ
KGSISVGQNVPFITGRVTGESANVNNPFQTIERQNVGISMSVFPVAMAGGNIVLDITSK
ADSLSSSTQASDVITNQRSIATTVNLRDGQTLLLGGLTDYKNTSQDSGVPFLSKIPLIG
LLFSSRSDSNEESTLYVLVKATIVRAL"
terminator 6266..6297
/label=tonB terminator
/note="bidirectional E. coli tonB-P14 transcription
terminator"
CDS complement(6396..7211)
/codon_start=1
/product="aminoglycoside phosphotransferase"
/label=KanR
/note="confers resistance to kanamycin in bacteria or G418
(Geneticin(R)) in eukaryotes"
/translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
primer_bind 7119..7138
/label=Kan-R
/note="Kanamycin resistance gene, reverse primer"
primer_bind 7203..7222
/label=pENTR-R
/note="pENTR vectors, reverse primer"
CDS complement(7569..8519)
/codon_start=1
/gene="rep101"
/product="RepA protein needed for replication with the
pSC101 origin"
/label=Rep101
/translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFGIFQWTNYAKFS
SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
rep_origin 8567..8788
/label=pSC101 ori
/note="low-copy replication origin that requires the Rep101
protein"
terminator complement(9280..9374)
/label=lambda t0 terminator
/note="transcription terminator from phage lambda"
ORIGIN
1 ctttttcttt aaaaccgaaa agattacttc gcgtttgcca cctgacgtct aagaaaagga
61 atattcagca atttgcccgt gccgaagaaa ggcccacccg tgaaggtgag ccagtgagtt
121 gattgctacg taattagtta gttagccctt agtgactcca gacgcgaatt atttttgatg
181 gcgttcctat tggttaaaaa atgagctgat ttaacaaaaa tttaatgcga attttaacaa
241 aatattaacg tttacaattt aaatatttgc ttatacaatc ttcctgtttt tggggctttt
301 ctgattatca accggggtac atatgattga catgctagtt ttacgattac cgttcatcga
361 ttctcttgtt tgctccagac tctcaggcaa tgacctgata gcctttgtag atctctcaaa
421 aatagctacc ctctccggca ttaatttatc agctagaacg gttgaatatc atattgatgg
481 tgatttgact gtctccggcc tttctcaccc ttttgaatct ttacctacac attactcagg
541 cattgcattt aaaatatatg agggttctaa aaatttttat ccttgcgttg aaataaaggc
601 ttctcccgca aaagtattac agggtcataa tgtttttggt acaaccgatt tagctttatg
661 ctctgaggct ttattgctta attttgctaa ttctttgcct tgcctgtatg atttattgga
721 tgttaatgct actactatta gtagaattga tgccaccttt tcagctcgcg ccccaaatga
781 aaatatagct aaacaggtta ttgaccattt gcgaaatgta tctaatggtc aaactaaatc
841 tactcgttcg cagaattggg aatcaactgt tatatggaat gaaacttcca gacaccgtac
901 tttagttgca tatttaaaac atgttgagct acagcattat attcagcaat taagctctaa
961 gccatccgca aaaatgacct cttatcaaaa ggagcaatta aaggtactct ctaatcctga
1021 cctgttggag tttgcttccg gtctggttcg ctttgaagct cgaattaaaa cgcgatattt
1081 gaagtctttc gggcttcctc ttaatctttt tgatgcaatc cgctttgctt ctgactataa
1141 tagtcagggt aaagacctga tttttgattt atggtcattc tcgttttctg aactgtttaa
1201 agcatttgag ggggattcaa tgaatattta tgacgattcc gcagtattgg acgctatcca
1261 gtctaaacat tttactatta ccccctctgg caaaacttct tttgcaaaag cctctcgcta
1321 ttttggtttt tatcgtcgtc tggtaaacga gggttatgat agtgttgctc ttactatgcc
1381 tcgtaattcc ttttggcgtt atgtatctgc attagttgaa tgtggtattc ctaaatctca
1441 actgatgaat ctttctacct gtaataatgt tgttccgtta gttcgtttta ttaacgtaga
1501 tttttcttcc caacgtcctg actggtataa tgagccagtt cttaaaatcg cataaggtaa
1561 ttcacaatga ttaaagttga aattaaacca tctcaagccc aatttactac tcgttctggt
1621 gtttctcgtc agggcaagcc ttattcactg aatgagcagc tttgttacgt tgatttgggt
1681 aatgaatatc cggttcttgt caagattact cttgatgaag gtcagccagc ctatgcgcct
1741 ggtctgtaca ccgttcatct gtcctctttc aaagttggtc agttcggttc ccttatgatt
1801 gaccgtctgc gcctcgttcc ggctaagtaa catggagcag gtcgcggatt tcgacacaat
1861 ttatcaggcg atgatacaaa tctccgttgt actttgtttc gcgcttggta taatcgctgg
1921 gggtcaaaga tgagtgtttt agtgtattct tttgcctctt tcgttttagg ttggtgcctt
1981 cgtagtggca ttacgtattt tacccgttta atggaaactt cctcatgaaa aagtctttag
2041 tcctcaaagc ctctgtagcc gttgctaccc tcgttccgat gctgtctttc gctgctgagg
2101 gtgacgatcc cgcaaaagcg gcctttaact ccctgcaagc ctcagcgacc gaatatatcg
2161 gttatgcgtg ggcgatggtt gttgtcattg tcggcgcaac tatcggtatc aagctgttta
2221 agaaattcac ctcgaaagca agctgataaa ccgatacaat taaaggctcc ttttggagcc
2281 ttttttttgg agattttcaa cgtgaaaaaa ttattattcg caattccttt agttgttcct
2341 ttctattctc actccgctga aactgttgaa agttgtttag caaaatccca tacagaaaat
2401 tcatttacta acgtctggaa agacgacaaa actttagatc gttacgctaa ctatgagggc
2461 tgtctgtgga atgctacagg cgttgtagtt tgtactggtg acgaaactca gtgttacggt
2521 acatgggttc ctattgggct tgctatccct gaaaatgagg gtggtggctc tgagggtggc
2581 ggttctgagg gtggcggttc tgagggtggc ggtactaaac ctcctgagta cggtgataca
2641 cctattccgg gctatactta tatcaaccct ctcgacggca cttatccgcc tggtactgag
2701 caaaaccccg ctaatcctaa tccttctctt gaggagtctc agcctcttaa tactttcatg
2761 tttcagaata ataggttccg aaataggcag ggggcattaa ctgtttatac gggcactgtt
2821 actcaaggca ctgaccccgt taaaacttat taccagtaca ctcctgtatc atcaaaagcc
2881 atgtatgacg cttactggaa cggtaaattc agagactgcg ctttccattc tggctttaat
2941 gaggatttat ttgtttgtga atatcaaggc caatcgtctg acctgcctca acctcctgtc
3001 aatgctggcg gcggctctgg tggtggttct ggtggcggct ctgagggtgg tggctctgag
3061 ggtggcggtt ctgagggtgg cggctctgag ggaggcggtt ccggtggtgg ctctggttcc
3121 ggtgattttg attatgaaaa gatggcaaac gctaataagg gggctatgac cgaaaatgcc
3181 gatgaaaacg cgctacagtc tgacgctaaa ggcaaacttg attctgtcgc tactgattac
3241 ggtgctgcta tcgatggttt cattggtgac gtttccggcc ttgctaatgg taatggtgct
3301 actggtgatt ttgctggctc taattcccaa atggctcaag tcggtgacgg tgataattca
3361 cctttaatga ataatttccg tcaatattta ccttccctcc ctcaatcggt tgaatgtcgc
3421 ccttttgtct ttggcgctgg taaaccatat gaattttcta ttgattgtga caaaataaac
3481 ttattccgtg gtgtctttgc gtttctttta tatgttgcca cctttatgta tgtattttct
3541 acgtttgcta acatactgcg taataaggag tcttaatcat gccagttctt ttgggtattc
3601 cgttattatt gcgtttcctc ggtttccttc tggtaacttt gttcggctat ctgcttactt
3661 ttcttaaaaa gggcttcggt aagatagcta ttgctatttc attgtttctt gctcttatta
3721 ttgggcttaa ctcaattctt gtgggttatc tctctgatat tagcgctcaa ttaccctctg
3781 actttgttca gggtgttcag ttaattctcc cgtctaatgc gcttccctgt ttttatgtta
3841 ttctctctgt aaaggctgct attttcattt ttgacgttaa acaaaaaatc gtttcttatt
3901 tggattggga taaataatat ggctgtttat tttgtaactg gcaaattagg ctctggaaag
3961 acgctcgtta gcgttggtaa gattcaggat aaaattgtag ctgggtgcaa aatagcaact
4021 aatcttgatt taaggcttca aaacctcccg caagtcggga ggttcgctaa aacgcctcgc
4081 gttcttagaa taccggataa gccttctata tctgatttgc ttgctattgg gcgcggtaat
4141 gattcctacg atgaaaataa aaacggcttg cttgttctcg atgagtgcgg tacttggttt
4201 aatacccgtt cttggaatga taaggaaaga cagccgatta ttgattggtt tctacatgct
4261 cgtaaattag gatgggatat tatttttctt gttcaggact tatctattgt tgataaacag
4321 gcgcgttctg cattagctga acatgttgtt tattgtcgtc gtctggacag aattacttta
4381 ccttttgtcg gtactttata ttctcttatt actggctcga aaatgcctct gcctaaatta
4441 catgttggcg ttgttaaata tggcgattct caattaagcc ctactgttga gcgttggctt
4501 tatactggta agaatttgta taacgcatat gatactaaac aggctttttc tagtaattat
4561 gattccggtg tttattctta tttaacgcct tatttatcac acggtcggta tttcaaacca
4621 ttaaatttag gtcagaagat gaaattaact aaaatatatt tgaaaaagtt ttctcgcgtt
4681 ctttgtcttg cgattggatt tgcatcagca tttacatata gttatataac ccaacctaag
4741 ccggaggtta aaaaggtagt ctctcagacc tatgattttg ataaattcac tattgactct
4801 tctcagcgtc ttaatctaag ctatcgctat gttttcaagg attctaaggg aaaattaatt
4861 aatagcgacg atttacagaa gcaaggttat tcactcacat atattgattt atgtactgtt
4921 tccattaaaa aaggtaattc aaatgaaatt gttaaatgta attaattttg ttttcttgat
4981 gtttgtttca tcatcttctt ttgctcaggt aattgaaatg aataattcgc ctctgcgcga
5041 ttttgtaact tggtattcaa agcaatcagg cgaatccgtt attgtttctc ccgatgtaaa
5101 aggtactgtt actgtatatt catctgacgt taaacctgaa aatctacgca atttctttat
5161 ttctgtttta cgtgcaaata attttgatat ggtaggttct aacccttcca ttattcagaa
5221 gtataatcca aacaatcagg attatattga tgaattgcca tcatctgata atcaggaata
5281 tgatgataat tccgctcctt ctggtggttt ctttgttccg caaaatgata atgttactca
5341 aacttttaaa attaataacg ttcgggcaaa ggatttaata cgagttgtcg aattgtttgt
5401 aaagtctaat acttctaaat cctcaaatgt attatctatt gacggctcta atctattagt
5461 tgttagtgct cctaaagata ttttagataa ccttcctcaa ttcctttcaa ctgttgattt
5521 gccaactgac cagatattga ttgagggttt gatatttgag gttcagcaag gtgatgcttt
5581 agatttttca tttgctgctg gctctcagcg tggcactgtt gcaggcggtg ttaatactga
5641 ccgcctcacc tctgttttat cttctgctgg tggttcgttc ggtattttta atggcgatgt
5701 tttagggcta tcagttcgcg cattaaagac taatagccat tcaaaaatat tgtctgtgcc
5761 acgtattctt acgctttcag gtcagaaggg ttctatctct gttggccaga atgtcccttt
5821 tattactggt cgtgtgactg gtgaatctgc caatgtaaat aatccatttc agacgattga
5881 gcgtcaaaat gtaggtattt ccatgagcgt ttttcctgtt gcaatggctg gcggtaatat
5941 tgttctggat attaccagca aggccgatag tttgagttct tctactcagg caagtgatgt
6001 tattactaat caaagaagta ttgctacaac ggttaatttg cgtgatggac agactctttt
6061 actcggtggc ctcactgatt ataaaaacac ttctcaggat tctggcgtac cgttcctgtc
6121 taaaatccct ttaatcggcc tcctgtttag ctcccgctct gattctaacg aggaaagcac
6181 gttatacgtg ctcgtcaaag caaccatagt acgcgccctg tagcatgcag gagtcactaa
6241 gggttagtta gttagattag cagaaagtca aaagcctccg accggaggct tttgactaaa
6301 acttcccttg gggttatcat tggggctcac tcaaaggcgg taatcagata aaaaaaatcc
6361 ttagctttcg ctaaggatga tttctgctag tattattaga aaaactcatc gagcatcaaa
6421 tgaaactgca atttattcat atcaggatta tcaataccat atttttgaaa aagccgtttc
6481 tgtaatgaag gagaaaactc accgaggcag ttccaaagaa tggcaaggtc ctggtaacgg
6541 tctgcgattc cgacccgtcc aacatcaata caacctatta atttcccctc gtcaaaaata
6601 aggttatcaa gtgagaaatc accatgagtg acgactgaat ccggtgagaa tggcaagagc
6661 ttgtgcattt ctttccagac ttgttcaaca ggccagccat tacgctcgtc atcaaaatca
6721 ctcgcatcaa ccaaaccgtt attcatgcgt gattgcgcct gagcaagacg aaatacacga
6781 tcgctgttaa aaggacaatt acaaacagga atcgaatgta accggcgcag gaacacggcc
6841 agcgcatcaa caatattttc acctgaatca ggatattctt ctaatacctg gaaggctgtt
6901 ttcccaggaa tcgcggtggt gagtaaccac gcatcatcag gagtacggat aaaatgcttg
6961 atggtcggga gaggcataaa ctccgtcagc cagttgagac ggaccatctc atctgtaaca
7021 tcattggcaa cgctaccttt gccatgtttc agaaacaact ctggcgcatc gggcttccca
7081 tacaagcgat agattgtcgc acctgattgc ccgacattat cgcgagccca tttataccca
7141 tataaatcag cgtccatgtt ggagtttaag cgcggacggg agcaagacgt ttcccgttga
7201 atatggctca taacacccct tgtattactg tttatgtaag cagacagttt tattgttcat
7261 gatgatatat ttttatcttg tgcaatgtaa catcagagat tttgagacac aacgtggctt
7321 tgttgaataa atcgaacttt tgctgagttg aaggatcagc tctagtagtt acattgtcga
7381 tctgttcatg gtgaacagct ttgaatgcac caaaaactcg taaaagctct gatgtatcta
7441 tcttttttac accgttttca tctgtgcata tggacagttt tccctttgat atgtaacggt
7501 gaacagttgt tctacttttg tttgttagtc ttgatgcttc actgatagat acaagagcca
7561 taagaacctc agatccttcc gtatttagcc agtatgttct ctagtgtggt tcgttgtttt
7621 tgcgtgagcc atgagaacga accattgaga tcatacttac tttgcatgtc actcaaaaat
7681 tttgcctcaa aactggtgag ctgaattttt gcagttaaag catcgtgtag tgtttttctt
7741 agtccgttat gtaggtagga atctgatgta atggttgttg gtattttgtc accattcatt
7801 tttatctggt tgttctcaag ttcggttacg agatccattt gtctatctag ttcaacttgg
7861 aaaatcaacg tatcagtcgg gcggcctcgc ttatcaacca ccaatttcat attgctgtaa
7921 gtgtttaaat ctttacttat tggtttcaaa acccattggt taagcctttt aaactcatgg
7981 tagttatttt caagcattaa catgaactta aattcatcaa ggctaatctc tatatttgcc
8041 ttgtgagttt tcttttgtgt tagttctttt aataaccact cataaatcct catagagtat
8101 ttgttttcaa aagacttaac atgttccaga ttatatttta tgaatttttt taactggaaa
8161 agataaggca atatctcttc actaaaaact aattctaatt tttcgcttga gaacttggca
8221 tagtttgtcc actggaaaat cccaaagcct ttaaccaaag gattcctgat ttccacagtt
8281 ctcgtcatca gctctctggt tgctttagct aatacaccat aagcattttc cctactgatg
8341 ttcatcatct gagcgtattg gttataagtg aacgataccg tccgttcttt ccttgtaggg
8401 ttttcaatcg tggggttgag tagtgccaca cagcataaaa ttagcttggt ttcatgctcc
8461 gttaagtcat agcgactaat cgctagttca tttgctttga aaacaactaa ttcagacata
8521 catctcaatt ggtctaggtg attttaatca ctataccaat tgagatgggc tagtcaatga
8581 taattacatg tccttttcct ttgagttgtg ggtatctgta aattctgcta gacctttgct
8641 ggaaaacttg taaattctgc tagaccctct gtaaattccg ctagaccttt gtgtgttttt
8701 ttgtttatat tcaagtggtt ataatttata gaataaagaa agaataaaaa aagataaaaa
8761 gaatagatcc cagccctgtg tataactcac tactttagtc agttccgcag tattacaaaa
8821 ggatgtcgca aacgctgttt gctcctctac aaaacagacc ttaaaaccct aaaggcttaa
8881 gtagcaccct cgcaagctcg ggcaaatcgc tgaatattcc ttttgtctcc gaccatcagg
8941 cacctgagtc gctgtctttt tcgtgacatt cagttcgctg cgctcacggc tctggcagtg
9001 aatgggggta aatggcacta caggcgcctt ttatggattc atgcaaggaa actacccata
9061 atacaagaaa agcccgtcac gggcttctca gggcgtttta tggcgggtct gctatgtggt
9121 gctatctgac tttttgctgt tcagcagttc ctgccctctg attttccagt ctgaccactt
9181 cggattatcc cgtgacaggt cattcagact ggctaatgca cccagtaagg cagcggtatc
9241 atcaacaggc ttacccgtct tactgtccct agtgcttgga ttctcaccaa taaaaaacgc
9301 ccggcggcaa ccgagcgttc tgaacaaatc cagatggagt tctgaggtca ttactggatc
9361 tatcaacagg agtccaagcg agctcgtaaa cttggtctga cagctctagc tccggcaaaa
9421 aaacgggcaa ggtgtcacca ccctgcc
//
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