| 质粒名称: | pAAV-CAG-LSSmScarlet3-hLAMP2A |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0000173 |
| 产品用途: | Expresses the LSSmScarlet3-hLAMP2A fusion of chemically stable red fluorescent protein with a large Stock's shift in the lysosomes of mammalian cells |
| 存储实验室: | Laboratory of Molecular Design |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV |
| 表达系统: | 腺病毒，哺乳动物表达 |
| 启动子: |
CAG
|
| 拷贝数: | 低拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | Mach1 |
|
插入基因信息 |
|
| 插入基因名称: | LSSmScarlet3-hLAMP2A |
| 物种来源: | Synthetic |
| 密码子优化: | 细菌表达优化 |
|
文献引用方法 |
|
| 参考文献部分: |
LSSmScarlet2 and LSSmScarlet3, Chemically Stable Genetically Encoded Red Fluorescent Proteins with a Large Stokes' Shift
Subach O.M., Vlaskina A.V., Agapova Y.K., Piatkevich K.D., Patrushev M.V., Samygina V.R., Subach F.V.
Int. J. Mol. Sci. 23, 11051 (2022)
|
买家导航
pAAV-CAG-LSSmScarlet3-hLAMP2A载体质粒基本信息
pAAV-CAG-LSSmScarlet3-hLAMP2A质粒图谱载体图谱和pAAV-CAG-LSSmScarlet3-hLAMP2A载体序列质粒序列多克隆位点信息
pAAV-CAG-LSSmScarlet3-hLAMP2A质粒载体简介
pAAV-CAG-LSSmScarlet3-hLAMP2A质粒序列载体序列
LOCUS pAAV-CAG-LSSmScarlet3-hLAMP2A 6715 bp DNA circular SYN 22-APR-2023
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6715)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Apr 29, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6715
/mol_type="other DNA"
/organism="synthetic DNA construct"
rep_origin complement(182..770)
/direction=LEFT
/label=ori
/note="high-copy-number colE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(941..1801)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(1802..1906)
/gene="bla"
/label=AmpR promoter
rep_origin complement(1932..2387)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 2942..3219
/label=chicken beta-actin promoter
regulatory 3637..3646
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
CDS 3643..4338
/codon_start=1
/label=LSSmScarlet3
/translation="MVSKGEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTA
KLKVTKGGPLPFSWDILSPQFMYGSRAFTKHPADIPDYHKQSFPEGFKWERVMNFEDGG
AVTVTQDTSLEDGTLIYEVKLRGTNFPPDGPVMQKKTMGLEADTERLYPEDGVLKGDIK
MALRLKDGGRYLAHVRTTYKAKKPVLMPGAYNVDRKLDITSHNEDYTVVEQFERSEGRH
STGDMDELYK"
CDS 4354..5583
/codon_start=1
/label=hLAMP2A
/translation="MVCFRLFPVPGSGLVLVCLVLGAVRSYALELNLTDSENATCLYAK
WQMNFTVRYETTNKTYKTVTISDHGTVTYNGSICGDDQNGPKIAVQFGPGFSWIANFTK
AASTYSIDSVSFSYNTGDNTTFPDAEDKGILTVDELLAIRIPLNDLFRCNSLSTLEKND
VVQHYWDVLVQAFVQNGTVSTNEFLCDKDKTSTVAPTIHTTVPSPTTTPTPKEKPEAGT
YSVNNGNDTCLLATMGLQLNITQDKVASVININPNTTHSTGSCRSHTALLRLNSSTIKY
LDFVFAVKNENRFYLKEVNISMYLVNGSVFSIANNNLSYWDAPLGSSYMCNKEQTVSVS
GAFQINTFDLRVQPFNVTQGKYSTAQDCSADDDNFLVPIAVGAALAGVLILVLLAYFIG
LKHHHAGYEQF"
primer_bind complement(4969..4988)
/label=hLAMP2A-R
misc_feature 5617..6205
/label=WPRE
/note="woodchuck posttranscriptional regulatory element"
CDS complement(6088..6099)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
polyA_signal 6248..6369
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
ORIGIN
1 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
61 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
121 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
181 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
241 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
301 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
361 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
421 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
481 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
541 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
601 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
661 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
721 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
781 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
841 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
901 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
961 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
1021 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
1081 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
1141 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
1201 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
1261 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
1321 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
1381 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
1441 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
1501 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
1561 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
1621 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
1681 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
1741 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
1801 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
1861 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
1921 aagtgccacc taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta
1981 aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga
2041 atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa
2101 cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga
2161 accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc
2221 taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga
2281 agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg
2341 cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtccc attcgccatt
2401 caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct
2461 gcgcgctcgc tcgctcactg aggccgcccg ggcaaagccc gggcgtcggg cgacctttgg
2521 tcgcccggcc tcagtgagcg agcgagcgcg cagagaggga gtggccaact ccatcactag
2581 gggttccttg tagttaatga ttaacccgcc atgctactta tctacgtagc catgctctag
2641 gaagatcgta ggttaattaa ggcgcgcccc taggggagat ggccagccag cccattgacg
2701 tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg
2761 gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt
2821 acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg
2881 accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg
2941 gtcgaggtga gccccacgtt ctgcttcact ctccccatct cccccccctc cccaccccca
3001 attttgtatt tatttatttt ttaattattt tgtgcagcga tgggggcggg gggggggggg
3061 gggcgcgcgc caggcggggc ggggcggggc gaggggcggg gcggggcgag gcggagaggt
3121 gcggcggcag ccaatcagag cggcgcgctc cgaaagtttc cttttatggc gaggcggcgg
3181 cggcggcggc cctataaaaa gcgaagcgcg cggcgggcgg gagtcgctgc gacgctgcct
3241 tcgccccgtg ccccgctccg ccgccgcctc gcgccgcccg ccccggctct gactgaccgc
3301 gttactccca caggtgagcg ggcgggacgg cccttctcct ccgggctgta attagcgctt
3361 ggtttaatga cggcttgttt cttttctgtg gctgcgtgaa agccttgagg ggctccggga
3421 gggccctttg tgcgggggga gcggctcggg gctgtccgcg gggggacggc tgccttcggg
3481 ggggacgggg cagggcgggg ttcggcttct ggcgtgtgac cggcggctct agagcctctg
3541 ctaaccatgt tcatgccttc ttctttttcc tacagctcct gggcaacgtg ctggttattg
3601 tgctgtctca tcattttggc aaagaattgg ggatccgcca ccatggtgag caagggcgag
3661 gcagtgatca aggagttcat gcggttcaag gtgcacatgg agggctccat gaacggccac
3721 gagttcgaga tcgagggcga gggcgagggc cgcccctacg agggcaccca gaccgccaag
3781 ctgaaggtga ccaagggtgg tccactgccc ttctcctggg acatcctgtc ccctcagttc
3841 atgtacggct ccagggcctt caccaagcac cccgccgata tccccgacta ccataagcag
3901 tccttccccg agggcttcaa gtgggagcgc gtgatgaact tcgaggacgg cggcgccgtg
3961 accgtgaccc aggacacctc cctggaggac ggcaccctga tctacgaggt gaagctccgc
4021 ggcaccaact tccctcctga cggccccgta atgcagaaga agactatggg cttggaagcg
4081 gacaccgagc ggttgtaccc cgaggacggc gtgctgaagg gcgacattaa gatggccctg
4141 cgcctgaagg acggcggccg ctacctggca cacgtcagga ccacctacaa ggccaagaag
4201 cccgtgttga tgcccggcgc ctacaacgtc gaccgcaagt tggacatcac ctcccacaac
4261 gaggactaca ccgtggtgga acagttcgaa cgctccgagg gccgccactc caccggcgac
4321 atggacgagc tgtacaagac cggtggcgga tcaatggtgt gcttccgcct cttcccggtt
4381 ccgggctcag ggctcgttct ggtctgccta gtcctgggag ctgtgcggtc ttatgcattg
4441 gaacttaatt tgacagattc agaaaatgcc acttgccttt atgcaaaatg gcagatgaat
4501 ttcacagtac gctatgaaac tacaaataaa acttataaaa ctgtaaccat ttcagaccat
4561 ggcactgtga catataatgg aagcatttgt ggggatgatc agaatggtcc caaaatagca
4621 gtgcagttcg gacctggctt ttcctggatt gcgaatttta ccaaggcagc atctacttat
4681 tcaattgaca gcgtctcatt ttcctacaac actggtgata acacaacatt tcctgatgct
4741 gaagataaag gaattcttac tgttgatgaa cttttggcca tcagaattcc attgaatgac
4801 ctttttagat gcaatagttt atcaactttg gaaaagaatg atgttgtcca acactactgg
4861 gatgttcttg tacaagcttt tgtccaaaat ggcacagtga gcacaaatga gttcctgtgt
4921 gataaagaca aaacttcaac agtggcaccc accatacaca ccactgtgcc atctcctact
4981 acaacaccta ctccaaagga aaaaccagaa gctggaacct attcagttaa taatggcaat
5041 gatacttgtc tgctggctac catggggctg cagctgaaca tcactcagga taaggttgct
5101 tcagttatta acatcaaccc caatacaact cactccacag gcagctgccg ttctcacact
5161 gctctactta gactcaatag cagcaccatt aagtatctag actttgtctt tgctgtgaaa
5221 aatgaaaacc gattttatct gaaggaagtg aacatcagca tgtatttggt taatggctcc
5281 gttttcagca ttgcaaataa caatctcagc tactgggatg cccccctggg aagttcttat
5341 atgtgcaaca aagagcagac tgtttcagtg tctggagcat ttcagataaa tacctttgat
5401 ctaagggttc agcctttcaa tgtgacacaa ggaaagtatt ctacagccca agactgcagt
5461 gcagatgacg acaacttcct tgtgcccata gcggtgggag ctgccttggc aggagtactt
5521 attctagtgt tgctggctta ttttattggt ctcaagcacc atcatgctgg atatgagcaa
5581 ttttagctcg aggaattcga tatcaagctt atcgataatc aacctctgga ttacaaaatt
5641 tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct
5701 gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg
5761 tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc
5821 gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt
5881 cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc
5941 gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg
6001 ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac ctggattctg
6061 cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc
6121 ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg
6181 atctcccttt gggccgcctc cccgcatcga taccgtcgac ccgggcggcc gcttcgagca
6241 gacatgataa gatacattga tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa
6301 tgctttattt gtgaaatttg tgatgctatt gctttatttg taaccattat aagctgcaat
6361 aaacaagtta acaacaacaa ttgcattcat tttatgtttc aggttcaggg ggagatgtgg
6421 gaggtttttt aaagcaagta aaacctctac aaatgtggta aaatcgataa ggatcttcct
6481 agagcatggc tacgtagata agtagcatgg cgggttaatc attaactaca aggaacccct
6541 agtgatggag ttggccactc cctctctgcg cgctcgctcg ctcactgagg ccgggcgacc
6601 aaaggtcgcc cgacgcccgg gctttgcccg ggcggcctca gtgagcgagc gagcgcgcag
6661 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgct
//
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