| 质粒名称: | pSL1395 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0002436 |
| 产品用途: | SB100X-specific transposon containing a constitutive PuroR expression unit and a light-inducible MCP/LIRP-dependent expression unit for SEAP-mRNA containing 8 tandem MS2-box repeats in the 5’-UTR (ITR(SB)-PRPBSA-PuroR-pA:PhCMV-(MS2)8-SEAP-pA-ITR(SB)). |
| 存储实验室: | Mingqi XIE Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pSBbi-BP |
| 表达系统: | 哺乳动物表达 |
| 启动子: |
PhCMV, PRPBSA
|
| 拷贝数: | 未拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | Stbl3 |
|
插入基因信息 |
|
| 插入基因名称: | PuroR, SEAP |
| 物种来源: | synthetic |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
Rationally designed light-inducible RNA-releasing protein for translational regulation and optogenetic control of gene therapies
NA
NA
|
pSL1395
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pSL1395载体质粒基本信息
pSL1395质粒图谱载体图谱和pSL1395载体序列质粒序列多克隆位点信息
pSL1395质粒载体简介
pSL1395质粒序列载体序列
LOCUS pSL1395 7114 bp DNA circular SYN 11-FEB-2026
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7114)
AUTHORS Trial User
TITLE Direct Submission
JOURNAL Exported Aug 30, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..7114
/mol_type="other DNA"
/organism="synthetic DNA construct"
primer_bind complement(1..20)
/label=oSCL513
terminator 22..249
/label=bGH polyA
misc_feature complement(333..364)
/label=SleepingBeauty-ITR_Li
/label=nonstandard type: ITR
misc_feature complement(530..560)
/label=SleepingBeauty-ITR_Lo
/label=nonstandard type: ITR
promoter 681..785
/label=AmpR promoter
misc_feature 786..1643
/label=AmpR
/label=nonstandard type: resistance
rep_origin 1741..2423
/label=ColE1 origin
misc_feature complement(2593..2624)
/label=SleepingBeauty-ITR-Ro
/label=nonstandard type: ITR
misc_feature 2790..2818
/label=SleepingBeauty-ITR_Ri
/label=nonstandard type: ITR
terminator complement(2954..3083)
/label=rBG_polyA
primer_bind 3060..3081
/label=rev-rBG
primer_bind 3152..3196
/label=oSCL636
primer_bind 3152..3171
/label=oSCL542
CDS complement(3175..3771)
/label=PuroR
primer_bind 3175..3196
/label=oSCL551
primer_bind complement(3752..3781)
/label=oSCL635
primer_bind complement(3757..3777)
/label=oSCL550
misc_feature complement(3772..3773)
/label=P2A
/label=nonstandard type: cleavage
primer_bind 3775..3807
/label=oSCL542
primer_bind 3786..3807
/label=oSCL506
misc_feature complement(3794..4405)
/label=P_RPBSA
/label=nonstandard type: promoter_const
primer_bind 4408..4427
/label=oSCL514
misc_feature 4415..4494
/label=P_EF1a
/label=nonstandard type: promoter_const
primer_bind 4476..4514
/label=oSCL1378
primer_bind 4476..4514
/label=oSCL2110
primer_bind 4494..4517
/label=oSCL1800
misc_feature 4496..5078
/label=CMV
primer_bind complement(5052..5083)
/label=OXB018
primer_bind 5059..5094
/label=oSCL1791
primer_bind 5062..5082
/label=oSCL1013
misc_feature 5084..5085
/label=linker
primer_bind 5089..5130
/label=oSCL2270
misc_feature 5112..5124
/label=linker
misc_feature 5130..5148
/label=MS2 box
misc_feature 5154..5163
/label=linker
misc_feature 5169..5187
/label=MS2 box
misc_feature 5193..5202
/label=linker
misc_feature 5193..5202
/label=linker
misc_feature 5201..5231
/label=hairpin
misc_feature 5207..5225
/label=MS2 box
misc_feature 5230..5242
/label=linker
misc_feature 5241..5271
/label=hairpin
misc_feature 5247..5265
/label=MS2 box
primer_bind complement(5264..5281)
/label=oSCL2271
primer_bind 5273..5299
/label=oSCL2270
misc_feature 5275..5276
/label=linker
misc_feature 5281..5293
/label=linker
misc_feature 5299..5317
/label=MS2 box
misc_feature 5323..5332
/label=linker
misc_feature 5338..5356
/label=MS2 box
misc_feature 5362..5371
/label=linker
misc_feature 5362..5371
/label=linker
misc_feature 5370..5400
/label=hairpin
misc_feature 5376..5394
/label=MS2 box
misc_feature 5399..5411
/label=linker
misc_feature 5410..5440
/label=hairpin
misc_feature 5416..5434
/label=MS2 box
primer_bind complement(5433..5462)
/label=oSCL2271
misc_feature 5444..5449
/label=linker
primer_bind 5457..5478
/label=Oscl31-3
misc_feature 5458..5461
/label=New Feature
misc_feature 5458..5461
/label=New Feature
misc_feature 5461
/label=EGFP
/note="note=""Fragment extracted from OWS233-Kpn1-EGFP-Xba1
[758 nt] : (KpnI[4] / XbaI[766])"
primer_bind 5464..5478
/label=oSCL775
primer_bind 5466..5478
/label=oSCL456
misc_feature 5472..7031
/label=SEAP
primer_bind complement(5660..5696)
/label=oSCL545
primer_bind 5677..5705
/label=oSCL544
primer_bind complement(6493..6524)
/label=oSCL546
primer_bind 6505..6537
/label=oSCL547
primer_bind 6590..6620
/label=Oscl50-1
primer_bind complement(6590..6620)
/label=Oscl50-2
primer_bind complement(6979..7031)
/label=oSCL795
primer_bind complement(7013..7029)
/label=oSCL2111
primer_bind complement(7013..7028)
/label=oSCL475
primer_bind complement(7015..7071)
/label=oSCL548
primer_bind complement(7015..7057)
/label=oSCL083
primer_bind complement(7015..7031)
/label=Oscl31-4
primer_bind complement(7015..7031)
/label=oSCL457
primer_bind complement(7015..7031)
/label=oSCL776
polyA_signal 7023..7031
/label=SV40 late polyA
misc_feature 7075..7080
/label=EcoRI
primer_bind complement(join(7114,1..20))
/label=oSCL2111
misc_feature 7114
/label=New Feature
misc_feature 7114
/label=New Feature
ORIGIN
1 ctagagctcg ctgatcagcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc
61 cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa
121 atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg
181 ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg gatgcggtgg
241 gctctatggc ttctgaggcg gaaagaacca gctgggccta ctagatagcg gaccccttac
301 cgaaacatcg ccgcattctg cagaggagtc gaacttagtg tatgtaaact tctgacccac
361 tggaattgtg atacagtgaa ttataagtga aataatctgt ctgtaaacaa ttgttggaaa
421 aatgacttgt gtcatgcaca aagtagatgt cctaactgac ttgccaaaac tattgtttgt
481 taacaagaaa tttgtggagt agttgaaaaa cgagttttaa tgactccaac ttaagtgtat
541 gtaaacttcc gacttcaact gtatagggat ccaattttaa caaaatatta acgcttacaa
601 tttcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatcaggtg
661 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
721 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
781 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
841 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
901 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
961 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
1021 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
1081 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
1141 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
1201 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
1261 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
1321 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
1381 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
1441 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
1501 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
1561 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
1621 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
1681 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
1741 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
1801 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
1861 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
1921 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
1981 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
2041 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
2101 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
2161 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
2221 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
2281 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
2341 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
2401 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
2461 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
2521 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
2581 cggatcccta tacagttgaa gtcggaagtt tacatacacc ttagccaaat acatttaaac
2641 tcactttttc acaattcctg acatttaatc ctagtaaaaa ttccctgtct taggtcagtt
2701 aggatcacca ctttatttta agaatgtgaa atatcagaat aatagtagag agaatgattc
2761 atttcagctt ttatttcttt catcacattc ccagtgggtc agaagtttac atacactcga
2821 ctcctctgca gaatgcggcg atgtttcggt aaggggtccc gagtagctag ttcatggcag
2881 ccagcatatg gcatatgttg ccaaactcta aaccaaatac tcattctgat gttttaaatg
2941 atttgccctc ccatatgtcc ttccgagtga gagacacaaa aaattccaac acactattgc
3001 aatgaaaata aatttccttt attagccaga agtcagatgc tcaaggggct tcatgatgtc
3061 cccataattt ttggcagagg gaaaaagatc tcagtggtat ttgtgagcca gggcattggc
3121 cacaccagcc accaccttct gataggcagc ctgcacctga ggagtgaatt ctcaggcacc
3181 gggcttgcgg gtcatgcacc aggtgcgcgg tccttcgggc acctcgacgt cggcggtgac
3241 ggtgaagccg agccgctcgt agaaggggag gttgcggggc gcggaggtct ccaggaaggc
3301 gggcaccccg gcgcgctcgg ccgcctccac tccggggagc acgacggcgc tgcccagacc
3361 cttgccctgg tggtcgggcg agacgccgac ggtggccagg aaccacgcgg gctccttggg
3421 ccggtgcggc gccaggaggc cttccatctg ttgctgcgcg gccagccggg aaccgctcaa
3481 ctcggccatg cgcgggccga tctcggcgaa caccgccccc gcttcgacgc tctccggcgt
3541 ggtccagacc gccaccgcgg cgccgtcgtc cgcgacccac accttgccga tgtcgagccc
3601 gacgcgcgtg aggaagagtt cttgcagctc ggtgacccgc tcgatgtggc ggtccggatc
3661 gacggtgtgg cgcgtggcgg ggtagtcggc gaacgcggcg gcgagggtgc gtacggccct
3721 ggggacgtcg tcgcgggtgg cgaggcgcac cgtgggcttg tactcggtca tggtggctag
3781 ctattcctca ggtgcagagg tttctacagg gaaagagaga aaggctacag ttagtttgct
3841 gtgatgatgc taagtgccga ggtcagatgt ctatggattg tacacggaat cccaacgcag
3901 gaaccaaaaa acactcaccc aaaaaaatgg cgctaaggcc gagagaaagg cgaagttccg
3961 tctacggcta tataccttga aacaggggcg gcagaagtga cgcaacgcgg ttgcgcggac
4021 ggcttcctgt gttttcattg gtcgaagcgt tctgcgcgtg cgcagagatt ccccagggaa
4081 ggcgggccct cttccgaatg tccaatccgg acattcttcc ggttggacct ccgcggtcag
4141 cgctgtgccc ggcgagagat cacgtggggc gcggaggcgg tgctgctggg gcacggccgt
4201 ccagcctcgg cggccatatt tttgaggggc tgttcatctc gttcacacgc tctgtccgcc
4261 atgtttgtga gtggaagcgc cattacccct tcaagcgact gaaggctgca gggcctctgg
4321 tggcccgcat ggggagacca gacccgccag gcccgccttt ccgcactcag tccgggctta
4381 ctttattttg tgagacaggg tctcgcctag aggctccggt gcccgtcagt gggcagagcg
4441 cacatcgccc acagtccccg agaagttggg gggaggggtc ggcaattgaa ccggagttga
4501 cattgattat tgactagtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca
4561 tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac
4621 gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact
4681 ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa
4741 gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg
4801 cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta
4861 gtcatcgcta ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg
4921 tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg
4981 caccaaaatc aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg
5041 ggcggtaggc gtgtacggtg ggaggtctat ataagcagag ctccttgaac cggtggatcc
5101 ggtctcaaac tgcaacaaac aaatcaccac cgtgaggatc acccacggtg gtgaaagcct
5161 tagcctagcc gtgaggatca cccacggtag gcacaagaaa ttctacccgt gaggatcacc
5221 cacgggtaga atcacaaaga ctacagccgt gaggatcacc cacggctgta gactcaaact
5281 gcaacaaaca aatcaccacc gtgaggatca cccacggtgg tgaaagcctt agcctagccg
5341 tgaggatcac ccacggtagg cacaagaaat tctacccgtg aggatcaccc acgggtagaa
5401 tcacaaagac tacagccgtg aggatcaccc acggctgtag actcaaactg gagacgaagc
5461 ttagcgccac catgctgctg ctgctgctgc tgctgggcct gaggctacag ctctccctgg
5521 gcatcatccc agttgaggag gagaacccgg acttctggaa ccgcgaggca gccgaggccc
5581 tgggtgccgc caagaagctg cagcctgcac agacagccgc caagaacctc atcatcttcc
5641 tgggcgatgg gatgggggtg tctacggtga cagctgccag aatcctaaaa gggcagaaga
5701 aggacaaact ggggcctgag atacccctgg ccatggaccg cttcccatat gtggctctgt
5761 ccaagacata caatgtagac aaacatgtgc cagacagtgg agccacagcc acggcctacc
5821 tgtgcggggt caagggcaac ttccagacca ttggcttgag tgcagccgcc cgctttaacc
5881 agtgcaacac gacacgcggc aacgaggtca tctccgtgat gaatcgggcc aagaaagcag
5941 ggaagtcagt gggagtggta accaccacac gagtgcagca cgcctcgcca gccggcacct
6001 acgcccacac ggtgaaccgc aactggtact cggacgccga cgtgcctgcc tcggcccgcc
6061 aggaggggtg ccaggacatc gctacgcagc tcatctccaa catggacatt gacgtgatcc
6121 taggtggagg ccgaaagtac atgtttcgca tgggaacccc agaccctgag tacccagatg
6181 actacagcca aggtgggacc aggctggacg ggaagaatct ggtgcaggaa tggctggcga
6241 agcgccaggg tgcccggtat gtgtggaacc gcactgagct catgcaggct tccctggacc
6301 cgtctgtgac ccatctcatg ggtctctttg agcctggaga catgaaatac gagatccacc
6361 gagactccac actggacccc tccctgatgg agatgacaga ggctgccctg cgcctgctga
6421 gcaggaaccc ccgcggcttc ttcctcttcg tggagggtgg tcgcatcgac catggtcatc
6481 atgaaagcag ggcttaccgg gcactgactg agaccatcat gttcgacgac gccattgaga
6541 gggcgggcca gctcaccagc gaggaggaca cgctgagcct cgtcactgcc gaccactccc
6601 acgtgttctc cttcggaggc taccccctgc gagggagctc catcttcggg ctggcccctg
6661 gcaaggcccg ggacaggaag gcctacacgg tcctcctata cggaaacggt ccaggctatg
6721 tgctcaagga cggcgcccgg ccggatgtta ccgagagcga gagcgggagc cccgagtatc
6781 ggcagcagtc agcagtgccc ctggacgaag agacccacgc aggcgaggac gtggcggtgt
6841 tcgcgcgcgg cccgcaggcg cacctggttc acggcgtgca ggagcagacc ttcatagcgc
6901 acgtcatggc cttcgccgcc tgcctggagc cctacaccgc ctgcgacctg gcgccccccg
6961 ccggcaccac cgacgccgcg cacccgggtt acagcagagt cggggcggcc ggccgcttcg
7021 agcagacatg agcggccgct caatctcgag gggatccact agtccagtgt ggtggaattc
7081 tgcagatatc cagcacagtg gcggccgctc gagt
//
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