| 质粒名称: | pAAV-hSyn-GRAB_NTS1.0 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0000157 |
| 产品用途: | Expresses the neurotensin sensor GRAB_NTS1.0 in neurons |
| 存储实验室: | Yulong Li Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV |
| 表达系统: | 腺病毒，哺乳动物表达 |
| 启动子: | hSyn |
| 拷贝数: | 低拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a，TOP10 |
|
插入基因信息 |
|
| 插入基因名称: | GRAB_NTS1.0 |
| 物种来源: | Synthetic |
| 密码子优化: | 哺乳细胞表达优化 |
|
文献引用方法 |
|
| 参考文献部分: |
A tool kit of highly selective and sensitive genetically encoded neuropeptide sensors
Wang H., Qian T., Zhao Y., Zhuo Y., Wu C., Osakada T., Chen P., Chen Z., Ren H., Yan Y., Geng L., Fu S., Mei L., Li G., Wu L., Jiang Y., Qian W., Zhang L., Peng W., Xu M., HU J., Jiang M., Chen L., Tang C., Zhu Y., Lin D., Zhou J., Li Y.
Science Vol. 382, 6672 (2023)
|
买家导航
pAAV-hSyn-GRAB_NTS1.0载体质粒基本信息
pAAV-hSyn-GRAB_NTS1.0质粒图谱载体图谱和pAAV-hSyn-GRAB_NTS1.0载体序列质粒序列多克隆位点信息
pAAV-hSyn-GRAB_NTS1.0质粒载体简介
pAAV-hSyn-GRAB_NTS1.0质粒序列载体序列
LOCUS pAAV-hSyn-GRAB_NTS1.0 6611 bp DNA circular SYN 21-APR-2023
DEFINITION Expresses the neurotensin sensor GRAB_NTS1.0 in neurons.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6611)
TITLE Yulong Li lab sensors
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 6611)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Apr 28, 2026 from SnapGene 6.0.2
https://www.biofeng.com
COMMENT SGRef: number: 1; type: "Journal Article"; journalName:
"Unpublished"
FEATURES Location/Qualifiers
source 1..6611
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR (alternate)
/note="Functional equivalent of wild-type AAV2 ITR"
promoter 131..574
/label=hSyn
regulatory 581..590
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
sig_peptide 587..649
/codon_start=1
/product="leader sequence from mouse immunoglobulin kappa
light chain"
/label=Ig-kappa leader
/translation="METDTLLLWVLLLWVPGSTGD"
protein_bind 650..674
/gene="mutant version of attB"
/label=attB1
/bound_moiety="BP Clonase(TM)"
/note="recombination site for the Gateway(R) BP reaction"
CDS 674..2713
/codon_start=1
/label=GRAB_NTS1.0
/translation="TTGRLNSSAPGTPGTPAADPFQRAQAGLEEALLAPGFGNASGNAS
ERVLAAPSSELDVNTDIYSKVLVTAVYLALFVVGTVGNTVTAFTLARKKSLQSLQSTVH
YHLGSLALSDLLTLLLAMPVELYNFIWVHHPWAFGDAGCRGYYFLRDACTYATALNVAS
LSVERYLAICHPFKAKTLMSRSRTKKFISAIWLASALLAVPMLFTMGEQNRSADGQHAG
GLVCTPTIHTATVKVVIQVNTFMSFIFPMVVISVLNTIIANKLTVMVRQAAEQLNGAPG
EPAPAGPRDTDALDLEEGGNVYIKADKQKNGIKAAFNIRHNIEDGGVQLAYHYQQNTPI
GDGPVLLPDNHYLSVQSKLSKDPNEKRDHMVLLEFVTAAGITWGMDELYKGGTGGSMVR
KGEELFTGVVPILVELDGDVNGHKFSVGGEGEGDATEGKLTLKFICTTGKLPVPWPTLV
TTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFKDDGNYKTRAEVKFEGDTLV
NRIELKGIDFKEDGNILGHKLEYNTGAAARWRGREPNRVQALRHGVRVLRAVVIAFVVC
WLPYHVRRLMFCYISDEQWTPFLYDFYHYFYMVTNALFYVSSTINPILYNLVSANFRHI
FLATLACLCPVWRRRRKRPAFSRKADSVSSNHTLSSNATRETLY"
primer_bind 1384..1411
/label=WKG157-F
primer_bind 1780..1801
/label=EGFP-C
/note="EGFP, forward primer"
primer_bind complement(1907..1928)
/label=EGFP-N
/note="EGFP, reverse primer"
primer_bind complement(2168..2187)
/label=EXFP-R
/note="For distinguishing EGFP variants, reverse primer"
misc_feature 2738..3326
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(2791..2811)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(3209..3220)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
polyA_signal 3358..3834
/label=hGH poly(A) signal
/note="human growth hormone polyadenylation signal"
primer_bind complement(3557..3576)
/label=hGH-PA-R
/note="Human growth hormone terminator, reverse primer"
repeat_region 3874..4014
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
repeat_region 3874..4003
/label=AAV2 ITR
rep_origin 4089..4544
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(4176..4195)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 4386..4407
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind complement(4561..4580)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind 4680..4702
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
primer_bind complement(4740..4758)
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
promoter 4826..4930
/gene="bla"
/label=AmpR promoter
CDS 4931..5791
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(5149..5168)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 5962..6550
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 6451..6470
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtatgagg cggggtgggg gtgcctacct gacgaccgac
181 cccgacccac tggacaagca cccaaccccc attccccaaa ttgcgcatcc cctatcagag
241 agggggaggg gaaacaggat gcggcgaggc gcgtgcgcac tgccagcttc agcaccgcgg
301 acagtgcctt cgcccccgcc tggcggcgcg cgccaccgcc gcctcagcac tgaaggcgcg
361 ctgacgtcac tcgccggtcc cccgcaaact ccccttcccg gccaccttgg tcgcgtccgc
421 gccgccgccg gcccagccgg accgcaccac gcgaggcgcg agataggggg gcacgggcgc
481 gaccatctgc gctgcggcgc cggcgactca gcgctgcctc agtctgcggt gggcagcgga
541 ggagtcgtgt cgtgcctgag agcgcagtcg agaaaccggt gccaccatgg agacagacac
601 actcctgcta tgggtactgc tgctctgggt tccaggttcc actggtgaca caagtttgta
661 caaaaaagtt ggcaccaccg gtcgcctcaa cagctccgcg ccgggaaccc cgggcacgcc
721 ggccgccgac cccttccagc gggcgcaggc cggactggag gaggcgctgc tggccccggg
781 cttcggcaac gcttcgggca acgcgtcgga gcgcgtcctg gcggcaccca gcagcgagct
841 ggacgtgaac accgacatct actccaaggt gctggtgacc gccgtgtacc tggcgctctt
901 cgtggtgggc acggtgggca acacggtgac ggcgttcacg ctggcgcgga agaagtcgct
961 gcagagcctg cagagcacgg tgcattacca cctgggcagc ctggcgctgt ccgacctgct
1021 caccctgctg ctggccatgc ccgtggagct gtacaacttc atctgggtgc accacccctg
1081 ggccttcggc gacgccggct gccgcggcta ctacttcctg cgcgacgcct gcacctacgc
1141 cacggccctc aacgtggcca gcctgagtgt ggagcgctac ctggccatct gccacccctt
1201 caaggccaag accctcatgt cccgaagccg caccaagaag ttcatcagcg ccatctggct
1261 cgcctcggcc ctgctggcgg tgcctatgct gttcaccatg ggcgagcaga accgcagcgc
1321 cgacggccag cacgccggcg gcctggtgtg cacccccacc atccacactg ccaccgtcaa
1381 ggtcgtcata caggtcaaca ccttcatgtc cttcatattc cccatggtgg tcatctcggt
1441 cctgaacacc atcatcgcca acaagctgac cgtcatggta cgccaggcgg ccgagcagct
1501 caacggcgcc cctggcgagc ccgcgccggc cgggccgcgc gacaccgacg cgctggacct
1561 ggaggaggga ggaaacgtct atatcaaggc cgacaagcag aagaacggca tcaaggcggc
1621 gttcaatatc cgccacaaca tcgaggacgg cggcgtgcag ctcgcctacc actaccagca
1681 gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcgtgca
1741 gtccaaactt tcgaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt
1801 gaccgccgcc gggatcactt ggggcatgga cgagctgtac aagggcggta ccggagggag
1861 catggtgaga aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga
1921 cggcgacgta aacggccaca agttcagcgt gggcggcgag ggtgagggcg atgccaccga
1981 gggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac
2041 cctcgtgacc accctgacct acggcgtgca gtgcttcagc cgctaccccg accacatgaa
2101 gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac atccaggagc gcaccatctt
2161 cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct
2221 ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca
2281 caagctggag tacaacaccg gagcagcagc acgctggcgc gggcgggagc ctaatagggt
2341 ccaggccctg cggcacggcg tgcgcgtcct acgtgcagtg gtcatcgcct ttgtggtctg
2401 ctggctgccc taccacgtgc ggcgcctcat gttctgctac atctcggatg agcagtggac
2461 tccgttcctc tatgacttct accactactt ctacatggtg accaacgcac tcttctacgt
2521 cagctccacc atcaacccca tcctgtacaa cctcgtctct gccaacttcc gccacatctt
2581 cctggccaca ctggcctgcc tctgcccggt gtggcggcgc aggaggaaga ggccagcctt
2641 ctcgaggaag gccgacagcg tgtccagcaa ccacaccctc tccagcaatg ccacccgcga
2701 gacgctgtac taggaattcg atatcaagct tatcgataat caacctctgg attacaaaat
2761 ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc
2821 tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt
2881 gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg
2941 cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg
3001 tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc
3061 cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt
3121 gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc tatgttgcca cctggattct
3181 gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg
3241 cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg
3301 gatctccctt tgggccgcct ccccgcatcg ataccgagcg ctgctcgaga gatctacggg
3361 tggcatccct gtgacccctc cccagtgcct ctcctggccc tggaagttgc cactccagtg
3421 cccaccagcc ttgtcctaat aaaattaagt tgcatcattt tgtctgacta ggtgtccttc
3481 tataatatta tggggtggag gggggtggta tggagcaagg ggcaagttgg gaagacaacc
3541 tgtagggcct gcggggtcta ttgggaacca agctggagtg cagtggcaca atcttggctc
3601 actgcaatct ccgcctcctg ggttcaagcg attctcctgc ctcagcctcc cgagttgttg
3661 ggattccagg catgcatgac caggctcagc taatttttgt ttttttggta gagacggggt
3721 ttcaccatat tggccaggct ggtctccaac tcctaatctc aggtgatcta cccaccttgg
3781 cctcccaaat tgctgggatt acaggcgtga accactgctc ccttccctgt ccttctgatt
3841 ttgtaggtaa ccacgtgcgg accgagcggc cgcaggaacc cctagtgatg gagttggcca
3901 ctccctctct gcgcgctcgc tcgctcactg aggccgggcg accaaaggtc gcccgacgcc
3961 cgggctttgc ccgggcggcc tcagtgagcg agcgagcgcg cagctgcctg caggggcgcc
4021 tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata cgtcaaagca
4081 accatagtac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag
4141 cgtgaccgct acacttgcca gcgccttagc gcccgctcct ttcgctttct tcccttcctt
4201 tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt
4261 ccgatttagt gctttacggc acctcgaccc caaaaaactt gatttgggtg atggttcacg
4321 tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt
4381 taatagtgga ctcttgttcc aaactggaac aacactcaac tctatctcgg gctattcttt
4441 tgatttataa gggattttgc cgatttcggt ctattggtta aaaaatgagc tgatttaaca
4501 aaaatttaac gcgaatttta acaaaatatt aacgtttaca attttatggt gcactctcag
4561 tacaatctgc tctgatgccg catagttaag ccagccccga cacccgccaa cacccgctga
4621 cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc
4681 cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg
4741 cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc
4801 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
4861 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
4921 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
4981 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
5041 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
5101 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
5161 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
5221 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
5281 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
5341 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
5401 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
5461 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
5521 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
5581 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
5641 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
5701 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
5761 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
5821 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
5881 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
5941 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
6001 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
6061 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
6121 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
6181 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
6241 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
6301 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
6361 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
6421 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
6481 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
6541 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
6601 tgctcacatg t
//
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