lenti_dCas9-KRAB-MeCP2

LOCUS       lenti_dCas9-KRAB-MeCP2               15398 bp ds-DNA     circular SYN 27-FEB-2019
DEFINITION  Bipartite dCas9 repressor (dCas9-KRAB-MeCP2) in a UCOE-SFFV-dCas9 
            lentiviral vector..
ACCESSION   .
VERSION     .
KEYWORDS    lenti_dCas9-KRAB-MeCP2
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15398)
  TITLE     Validation of VIPER-inferred master regulators by pooled CRISPR 
            screening
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 15398)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Feb 27, 2019 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..15398
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        103..510
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     regulatory      579..588
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             585..4688
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             4692..4718
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             4737..4757
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4764..4784
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4824..5009
                     /codon_start=1
                     /gene="ZNF10"
                     /gene_synonym="KOX1"
                     /product="Kruppel-associated box (KRAB) transcriptional 
                     repression domain from the human zinc finger protein ZNF10 
                     (Margolin et al., 1994)"
                     /label=KRAB
                     /translation="RTLVTFKDVFVDFTREEWKLLDTAQQIVYRNVMLENYKNLVSLGY
                     QLTKPDVILRLEKGEEP"
     CDS             5043..5063
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5550..5570
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=7xHis
                     /translation="HHHHHHH"
     CDS             5940..5996
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             5997..6395
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    6456..7044
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6509..6529)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6927..6938)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(7047..7063)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7048..7064)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             7154..7387
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(7412..7430)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(7413..7430)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(7720..7739)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(7953..7972)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8072..8094
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8132..8150)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8218..8322
                     /gene="bla"
                     /label=AmpR promoter
     CDS             8323..9183
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8541..8560)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9354..9942
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9843..9862
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10096..10113
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(10183..10203)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        10188..10517
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      10368..10503
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     10430..10449
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     intron          11651..11716
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             11846..11866
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    12291..12425
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(12328..12347)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     12382..12401
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             12593..13226
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    13273..13398
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    13895..14128
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    14642..14759
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 gcgcggagtc ctgcagcccc gataaaataa aagattttat ttagtctcca gaaaaagggg
       61 ggaatgaaag accccacctg taggtttggc aagctagctg cagtaacgcc attttgcaag
      121 gcatggaaaa ataccaaacc aagaatagag aagttcagat caagggcggg tacatgaaaa
      181 tagctaacgt tgggccaaac aggatatctg cggtgagcag tttcggcccc ggcccggggc
      241 caagaacaga tggtcaccgc agtttcggcc ccggcccgag gccaagaaca gatggtcccc
      301 agatatggcc caaccctcag cagtttctta agacccatca gatgtttcca ggctccccca
      361 aggacctgaa atgaccctgc gccttatttg aattaaccaa tcagcctgct tctcgcttct
      421 gttcgcgcgc ttctgcttcc cgagctctat aaaagagctc acaacccctc actcggcgcg
      481 ccagtcctcc gacagactga gtcgcccggg ggggatctgg agctctcgag aattctcacg
      541 cgtctgcagg atatcaagct tgcggtaccg cgggcccggc caccatggac aagaagtaca
      601 gcatcggcct ggccatcggc accaactctg tgggctgggc cgtgatcacc gacgagtaca
      661 aggtgcccag caagaaattc aaggtgctgg gcaacaccga ccggcacagc atcaagaaga
      721 acctgatcgg cgccctgctg ttcgacagcg gagaaacagc cgaggccacc cggctgaaga
      781 gaaccgccag aagaagatac accagacgga agaaccggat ctgctatctg caagagatct
      841 tcagcaacga gatggccaag gtggacgaca gcttcttcca cagactggaa gagtccttcc
      901 tggtggaaga ggataagaag cacgagcggc accccatctt cggcaacatc gtggacgagg
      961 tggcctacca cgagaagtac cccaccatct accacctgag aaagaaactg gtggacagca
     1021 ccgacaaggc cgacctgcgg ctgatctatc tggccctggc ccacatgatc aagttccggg
     1081 gccacttcct gatcgagggc gacctgaacc ccgacaacag cgacgtggac aagctgttca
     1141 tccagctggt gcagacctac aaccagctgt tcgaggaaaa ccccatcaac gccagcggcg
     1201 tggacgccaa ggccatcctg tctgccagac tgagcaagag cagacggctg gaaaatctga
     1261 tcgcccagct gcccggcgag aagaagaatg gcctgttcgg caacctgatt gccctgagcc
     1321 tgggcctgac ccccaacttc aagagcaact tcgacctggc cgaggatgcc aaactgcagc
     1381 tgagcaagga cacctacgac gacgacctgg acaacctgct ggcccagatc ggcgaccagt
     1441 acgccgacct gtttctggcc gccaagaacc tgtccgacgc catcctgctg agcgacatcc
     1501 tgagagtgaa caccgagatc accaaggccc ccctgagcgc ctctatgatc aagagatacg
     1561 acgagcacca ccaggacctg accctgctga aagctctcgt gcggcagcag ctgcctgaga
     1621 agtacaaaga gattttcttc gaccagagca agaacggcta cgccggctac atcgatggcg
     1681 gagccagcca ggaagagttc tacaagttca tcaagcccat cctggaaaag atggacggca
     1741 ccgaggaact gctcgtgaag ctgaacagag aggacctgct gcggaagcag cggaccttcg
     1801 acaacggcag catcccccac cagatccacc tgggagagct gcacgccatt ctgcggcggc
     1861 aggaagattt ttacccattc ctgaaggaca accgggaaaa gatcgagaag atcctgacct
     1921 tccgcatccc ctactacgtg ggccctctgg ccaggggaaa cagcagattc gcctggatga
     1981 ccagaaagag cgaggaaacc atcaccccct ggaacttcga ggaagtggtg gacaagggcg
     2041 ccagcgccca gagcttcatc gagcggatga ccaacttcga taagaacctg cccaacgaga
     2101 aggtgctgcc caagcacagc ctgctgtacg agtacttcac cgtgtacaac gagctgacca
     2161 aagtgaaata cgtgaccgag ggaatgagaa agcccgcctt cctgagcggc gagcagaaaa
     2221 aagccatcgt ggacctgctg ttcaagacca accggaaagt gaccgtgaag cagctgaaag
     2281 aggactactt caagaaaatc gagtgcttcg actccgtgga aatctccggc gtggaagatc
     2341 ggttcaacgc ctccctgggc acataccacg atctgctgaa aattatcaag gacaaggact
     2401 tcctggacaa tgaggaaaac gaggacattc tggaagatat cgtgctgacc ctgacactgt
     2461 ttgaggacag agagatgatc gaggaacggc tgaaaaccta tgcccacctg ttcgacgaca
     2521 aagtgatgaa gcagctgaag cggcggagat acaccggctg gggcaggctg agccggaagc
     2581 tgatcaacgg catccgggac aagcagtccg gcaagacaat cctggatttc ctgaagtccg
     2641 acggcttcgc caacagaaac ttcatgcagc tgatccacga cgacagcctg acctttaaag
     2701 aggacatcca gaaagcccag gtgtccggcc agggcgatag cctgcacgag cacattgcca
     2761 atctggccgg cagccccgcc attaagaagg gcatcctgca gacagtgaag gtggtggacg
     2821 agctcgtgaa agtgatgggc cggcacaagc ccgagaacat cgtgatcgaa atggccagag
     2881 agaaccagac cacccagaag ggacagaaga acagccgcga gagaatgaag cggatcgaag
     2941 agggcatcaa agagctgggc agccagatcc tgaaagaaca ccccgtggaa aacacccagc
     3001 tgcagaacga gaagctgtac ctgtactacc tgcagaatgg gcgggatatg tacgtggacc
     3061 aggaactgga catcaaccgg ctgtccgact acgatgtgga cgctatcgtg cctcagagct
     3121 ttctgaagga cgactccatc gataacaaag tgctgactcg gagcgacaag aaccggggca
     3181 agagcgacaa cgtgccctcc gaagaggtcg tgaagaagat gaagaactac tggcgccagc
     3241 tgctgaatgc caagctgatt acccagagga agttcgacaa tctgaccaag gccgagagag
     3301 gcggcctgag cgaactggat aaggccggct tcatcaagag acagctggtg gaaacccggc
     3361 agatcacaaa gcacgtggca cagatcctgg actcccggat gaacactaag tacgacgaga
     3421 acgacaaact gatccgggaa gtgaaagtga tcaccctgaa gtccaagctg gtgtccgatt
     3481 tccggaagga tttccagttt tacaaagtgc gcgagatcaa caactaccac cacgcccacg
     3541 acgcctacct gaacgccgtc gtgggaaccg ccctgatcaa aaagtaccct aagctggaaa
     3601 gcgagttcgt gtacggcgac tacaaggtgt acgacgtgcg gaagatgatc gccaagagcg
     3661 agcaggaaat cggcaaggct accgccaagt acttcttcta cagcaacatc atgaactttt
     3721 tcaagaccga gattaccctg gccaacggcg agatccggaa gcggcctctg atcgagacaa
     3781 acggcgaaac aggcgagatc gtgtgggata agggccggga ctttgccacc gtgcggaaag
     3841 tgctgtctat gccccaagtg aatatcgtga aaaagaccga ggtgcagaca ggcggcttca
     3901 gcaaagagtc tatcctgccc aagaggaaca gcgacaagct gatcgccaga aagaaggact
     3961 gggaccctaa gaagtacggc ggcttcgaca gccccaccgt ggcctattct gtgctggtgg
     4021 tggccaaagt ggaaaagggc aagtccaaga aactgaagag tgtgaaagag ctgctgggga
     4081 tcaccatcat ggaaagaagc agcttcgaga agaatcccat cgactttctg gaagccaagg
     4141 gctacaaaga agtgaaaaag gacctgatca tcaagctgcc taagtactcc ctgttcgagc
     4201 tggaaaacgg ccggaagaga atgctggcct ctgccggcga actgcagaag ggaaacgaac
     4261 tggccctgcc ctccaaatat gtgaacttcc tgtacctggc cagccactat gagaagctga
     4321 agggctcccc cgaggataat gagcagaaac agctgtttgt ggaacagcac aaacactacc
     4381 tggacgagat catcgagcag atcagcgagt tctccaagag agtgatcctg gccgacgcta
     4441 atctggacaa ggtgctgagc gcctacaaca agcacagaga caagcctatc agagagcagg
     4501 ccgagaatat catccacctg tttaccctga ccaatctggg agcccctgcc gccttcaagt
     4561 actttgacac caccatcgac cggaagaggt acaccagcac caaagaggtg ctggacgcca
     4621 ccctgatcca ccagagcatc accggcctgt acgagacacg gatcgacctg tctcagctgg
     4681 gaggcgacgc ctatccctat gacgtgcccg attatgccag cctgggcagc ggctccccca
     4741 agaaaaaacg caaggtggaa gatcctaaga aaaagcggaa agtggacggc attggtagtg
     4801 ggagcaacgg cagcagcgga tcccggacac tggtgacctt caaggatgta tttgtggact
     4861 tcaccaggga ggagtggaag ctgctggaca ctgctcagca gatcgtgtac agaaatgtga
     4921 tgctggagaa ctataagaac ctggtttcct tgggttatca gcttactaag ccagatgtga
     4981 tcctccggtt ggagaaggga gaagagccct cgggaggtgg ttcgggtggc tctggatcaa
     5041 gcccaaagaa gaaacggaag gtggaagcct cagtgcaggt gaaaagggtg ctggaaaaat
     5101 cccccggcaa actcctcgtg aagatgccct tccaggcttc ccctggcgga aaaggtgaag
     5161 ggggtggcgc aaccacatct gcccaggtca tggtcatcaa gcgacctgga aggaaaagaa
     5221 aggccgaggc tgaccctcag gccattccaa agaaacgggg acgcaagcca gggtccgtgg
     5281 tcgcagctgc agcagctgag gctaagaaaa aggcagtgaa ggaaagctcc atccgcagtg
     5341 tgcaggagac tgtcctgccc atcaagaaga ggaagactag ggagaccgtg tccatcgagg
     5401 tcaaagaagt ggtcaagccc ctgctcgtgt ccaccctggg cgaaaaatct ggaaaggggc
     5461 tcaaaacatg caagtcacct ggacggaaaa gcaaggagtc tagtccaaag gggcgctcaa
     5521 gctccgcttc tagtccccct aaaaaggaac accatcacca tcaccatcac gccgagtctc
     5581 ctaaggctcc tatgccactg ctcccaccac ctccaccacc tgagccacag tcaagcgaag
     5641 accccatcag cccacccgag cctcaggatc tgtcctctag tatttgcaaa gaggaaaaga
     5701 tgcccagagc aggcagcctg gagagtgatg gctgtccaaa agaacccgcc aagacccagc
     5761 ctatggtggc agccgctgca actaccacca caaccacaac taccacagtg gccgaaaaat
     5821 acaagcatcg cggcgagggc gaacgaaagg acattgtgtc aagctccatg cccagaccta
     5881 accgggagga accagtcgat agtaggacac ccgtgactga gagagtctca gcggccgccg
     5941 caacaaactt ctctctgctg aaacaagccg gagatgtcga agagaatcct ggaccgatgg
     6001 ccaagccttt gtctcaagaa gaatccaccc tcattgaaag agcaacggct acaatcaaca
     6061 gcatccccat ctctgaagac tacagcgtcg ccagcgcagc tctctctagc gacggccgca
     6121 tcttcactgg tgtcaatgta tatcatttta ctgggggacc ttgtgcagaa ctcgtggtgc
     6181 tgggcactgc tgctgctgcg gcagctggca acctgacttg tatcgtcgcg atcggaaatg
     6241 agaacagggg catcttgagc ccctgcggac ggtgccgaca ggtgcttctc gatctgcatc
     6301 ctgggatcaa agccatagtg aaggacagtg atggacagcc gacggcagtt gggattcgtg
     6361 aattgctgcc ctctggttat gtgtgggagg gctaataagg ccgcgactct agagtcgacc
     6421 tgcaggcatg caagcttgat atcaagctta tcgataatca acctctggat tacaaaattt
     6481 gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg
     6541 ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt
     6601 ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg
     6661 tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc
     6721 agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg
     6781 cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt
     6841 tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg tgttgccacc tggattctgc
     6901 gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg
     6961 gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga
     7021 tctccctttg ggccgcctcc ccgcatcgat accgtcgacc tcgagggaat taattcgagc
     7081 tcggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa
     7141 gaaaaggggg gactggaagg gctaattcac tcccaacgaa gacaagatct gctttttgct
     7201 tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg
     7261 aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt
     7321 ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc
     7381 tctagcagca tctagaatta attccgtgta ttctatagtg tcacctaaat cgtatgtgta
     7441 tgatacataa ggttatgtat taattgtagc cgcgttctaa cgacaatatg tacaagccta
     7501 attgtgtagc atctggctta ctgaagcaga ccctatcatc tctctcgtaa actgccgtca
     7561 gagtcggttt ggttggacga accttctgag tttctggtaa cgccgtcccg cacccggaaa
     7621 tggtcagcga accaatcagc agggtcatcg ctagccagat cctctacgcc ggacgcatcg
     7681 tggccggcat caccggcgcc acaggtgcgg ttgctggcgc ctatatcgcc gacatcaccg
     7741 atggggaaga tcgggctcgc cacttcgggc tcatgagcgc ttgtttcggc gtgggtatgg
     7801 tggcaggccc cgtggccggg ggactgttgg gcgccatctc cttgcatgca ccattccttg
     7861 cggcggcggt gctcaacggc ctcaacctac tactgggctg cttcctaatg caggagtcgc
     7921 ataagggaga gcgtcgaatg gtgcactctc agtacaatct gctctgatgc cgcatagtta
     7981 agccagcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg
     8041 gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca
     8101 ccgtcatcac cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt tttataggtt
     8161 aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc
     8221 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
     8281 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc
     8341 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
     8401 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa
     8461 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg
     8521 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa
     8581 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
     8641 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
     8701 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta
     8761 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
     8821 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca
     8881 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
     8941 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc
     9001 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca
     9061 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca
     9121 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
     9181 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa
     9241 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
     9301 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     9361 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     9421 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     9481 gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac
     9541 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     9601 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     9661 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     9721 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     9781 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     9841 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     9901 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     9961 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
    10021 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc
    10081 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa
    10141 ccgcctctcc ccgcgcgttg gccgattcat taatgcagct gtggaatgtg tgtcagttag
    10201 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
    10261 agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
    10321 tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa
    10381 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag
    10441 aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag
    10501 gcctaggctt ttgcaaaaag cttggacaca agacaggctt gcgagatatg tttgagaata
    10561 ccactttatc ccgcgtcagg gagaggcagt gcgtaaaaag acgcggactc atgtgaaata
    10621 ctggttttta gtgcgccaga tctctataat ctcgcgcaac ctattttccc ctcgaacact
    10681 ttttaagccg tagataaaca ggctgggaca cttcacatga gcgaaaaata catcgtcacc
    10741 tgggacatgt tgcagatcca tgcacgtaaa ctcgcaagcc gactgatgcc ttctgaacaa
    10801 tggaaaggca ttattgccgt aagccgtggc ggtctgtacc gggtgcgtta ctggcgcgtg
    10861 aactgggtat tcgtcatgtc gataccgttt gtatttccag ctacgatcac gacaaccagc
    10921 gcgagcttaa agtgctgaaa cgcgcagaag gcgatggcga aggcttcatc gttattgatg
    10981 acctggtgga taccggtggt actgcggttg cgattcgtga aatgtatcca aaagcgcact
    11041 ttgtcaccat cttcgcaaaa ccggctggtc gtccgctggt tgatgactat gttgttgata
    11101 tcccgcaaga tacctggatt gaacagccgt gggatatggg cgtcgtattc gtcccgccaa
    11161 tctccggtcg ctaatctttt caacgcctgg cactgccggg cgttgttctt tttaacttca
    11221 ggcgggttac aatagtttcc agtaagtatt ctggaggctg catccatgac acaggcaaac
    11281 ctgagcgaaa ccctgttcaa accccgcttt aaacatcctg aaacctcgac gctagtccgc
    11341 cgctttaatc acggcgcaca accgcctgtg cagtcggccc ttgatggtaa aaccatccct
    11401 cactggtatc gcatgattaa ccgtctgatg tggatctggc gcggcattga cccacgcgaa
    11461 atcctcgacg tccaggcacg tattgtgatg agcgatgccg aacgtaccga cgatgattta
    11521 tacgatacgg tgattggcta ccgtggcggc aactggattt atgagtgggc cccggatctt
    11581 tgtgaaggaa ccttacttct gtggtgtgac ataattggac aaactaccta cagagattta
    11641 aagctctaag gtaaatataa aatttttaag tgtataatgt gttaaactac tgattctaat
    11701 tgtttgtgta ttttagattc caacctatgg aactgatgaa tgggagcagt ggtggaatgc
    11761 ctttaatgag gaaaacctgt tttgctcaga agaaatgcca tctagtgatg atgaggctac
    11821 tgctgactct caacattcta ctcctccaaa aaagaagaga aaggtagaag accccaagga
    11881 ctttccttca gaattgctaa gttttttgag tcatgctgtg tttagtaata gaactcttgc
    11941 ttgctttgct atttacacca caaaggaaaa agctgcactg ctatacaaga aaattatgga
    12001 aaaatattct gtaaccttta taagtaggca taacagttat aatcataaca tactgttttt
    12061 tcttactcca cacaggcata gagtgtctgc tattaataac tatgctcaaa aattgtgtac
    12121 ctttagcttt ttaatttgta aaggggttaa taaggaatat ttgatgtata gtgccttgac
    12181 tagagatcat aatcagccat accacatttg tagaggtttt acttgcttta aaaaacctcc
    12241 cacacctccc cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt aacttgttta
    12301 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
    12361 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct
    12421 ggatcaactg gataactcaa gctaaccaaa atcatcccaa acttcccacc ccatacccta
    12481 ttaccactgc caattacctg tggtttcatt tactctaaac ctgtgattcc tctgaattat
    12541 tttcatttta aagaaattgt atttgttaaa tatgtactac aaacttagta gttggaaggg
    12601 ctaattcact cccaaagaag acaagatatc cttgatctgt ggatctacca cacacaaggc
    12661 tacttccctg attagcagaa ctacacacca gggccagggg tcagatatcc actgaccttt
    12721 ggatggtgct acaagctagt accagttgag ccagataagg tagaagaggc caataaagga
    12781 gagaacacca gcttgttaca ccctgtgagc ctgcatggga tggatgaccc ggagagagaa
    12841 gtgttagagt ggaggtttga cagccgccta gcatttcatc acgtggcccg agagctgcat
    12901 ccggagtact tcaagaactg ctgatatcga gcttgctaca agggactttc cgctggggac
    12961 tttccaggga ggcgtggcct gggcgggact ggggagtggc gagccctcag atcctgcata
    13021 taagcagctg ctttttgcct gtactgggtc tctctggtta gaccagatct gagcctggga
    13081 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct
    13141 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt
    13201 ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa agcgaaaggg
    13261 aaaccagagg agctctctcg acgcaggact cggcttgctg aagcgcgcac ggcaagaggc
    13321 gaggggcggc gactggtgag tacgccaaaa attttgacta gcggaggcta gaaggagaga
    13381 gatgggtgcg agagcgtcag tattaagcgg gggagaatta gatcgcgatg ggaaaaaatt
    13441 cggttaaggc cagggggaaa gaaaaaatat aaattaaaac atatagtatg ggcaagcagg
    13501 gagctagaac gattcgcagt taatcctggc ctgttagaaa catcagaagg ctgtagacaa
    13561 atactgggac agctacaacc atcccttcag acaggatcag aagaacttag atcattatat
    13621 aatacagtag caaccctcta ttgtgtgcat caaaggatag agataaaaga caccaaggaa
    13681 gctttagaca agatagagga agagcaaaac aaaagtaaga ccaccgcaca gcaagcggcc
    13741 ggccgctgat cttcagacct ggaggaggag atatgaggga caattggaga agtgaattat
    13801 ataaatataa agtagtaaaa attgaaccat taggagtagc acccaccaag gcaaagagaa
    13861 gagtggtgca gagagaaaaa agagcagtgg gaataggagc tttgttcctt gggttcttgg
    13921 gagcagcagg aagcactatg ggcgcagcgt caatgacgct gacggtacag gccagacaat
    13981 tattgtctgg tatagtgcag cagcagaaca atttgctgag ggctattgag gcgcaacagc
    14041 atctgttgca actcacagtc tggggcatca agcagctcca ggcaagaatc ctggctgtgg
    14101 aaagatacct aaaggatcaa cagctcctgg ggatttgggg ttgctctgga aaactcattt
    14161 gcaccactgc tgtgccttgg aatgctagtt ggagtaataa atctctggaa cagatttgga
    14221 atcacacgac ctggatggag tgggacagag aaattaacaa ttacacaagc ttaatacact
    14281 ccttaattga agaatcgcaa aaccagcaag aaaagaatga acaagaatta ttggaattag
    14341 ataaatgggc aagtttgtgg aattggttta acataacaaa ttggctgtgg tatataaaat
    14401 tattcataat gatagtagga ggcttggtag gtttaagaat agtttttgct gtactttcta
    14461 tagtgaatag agttaggcag ggatattcac cattatcgtt tcagacccac ctcccaaccc
    14521 cgaggggacc cgacaggccc gaaggaatag aagaagaagg tggagagaga gacagagaca
    14581 gatccattcg attagtgaac ggatctcgac ggtatcgcca aatggcagta ttcatccaca
    14641 attttaaaag aaaagggggg attggggggt acagtgcagg ggaaagaata gtagacataa
    14701 tagcaacaga catacaaact aaagaattac aaaaacaaat tacaaaaatt caaaattttc
    14761 gggtttatta cagggacagc agagatccag tttggatcga taagcttgat atcgaattcg
    14821 ggaggtggtc cctgcagtta cgccaatgat aacccccgcc agaaaaatct tagtagcctt
    14881 ccctttttgt tttccgtgcc ccaactcggc ggattgactc ggccccttcc ggaaacaccc
    14941 gaatcaactt ctagtcaaat tattgttcac gccgcaatga cccacccctg gcccgcgtct
    15001 gtggaactga cccctggtgt acaggagagt tcgctgctga aagtggtccc aaaggggtac
    15061 tagtttttaa gctcccaact ccccctcccc cagcgtctgg aggattccac accctcgcac
    15121 cgcaggggcg aggaagtggg cggagtccgg ttttggcgcc agccgctgag gctgccaagc
    15181 agaaaagcca ccgctgagga gactccggtc actgtcctcg ccccgcctcc cccttccctc
    15241 cccttgggga ccaccgggcg ccacgccgcg aacggtaagt gccgcggtcg tcggcgcctc
    15301 cgccctcccc ctagggcccc aattcccagc gggcgcggcg cgcggccccg cgcgcagctc
    15361 ccggctccct cccccttcgg atgtggcttg agctgtag
//