pimEJ5GFP

LOCUS       pimEJ5GFP               12719 bp ds-DNA     circular SYN 06-AUG-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pimEJ5GFP
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12719)
  AUTHORS   Bennardo N, Cheng A, Huang N, Stark JM
  TITLE     Alternative-NHEJ is a mechanistically distinct pathway of mammalian 
            chromosome break repair.
  JOURNAL   PLoS Genet. 2008 Jun 27;4(6):e1000110. doi: 
            10.1371/journal.pgen.1000110.
  PUBMED    18584027
REFERENCE   2  (bases 1 to 12719)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 6, 2018 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..12719
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     1..20
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1..19
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             557..1579
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     enhancer        1733..2112
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2114..2389
                     /label=chicken beta-actin promoter
     intron          2390..3398
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     3322..3344
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     3406..3425
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     promoter        3480..3977
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(3515..3538)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     3910..3929
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             3996..4595
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3996..4015)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4492..4512
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     regulatory      5260..5269
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             5266..5985
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5311..5332)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5572..5591)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5919..5940
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(6190..6209)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_site      6255..6310
                     /label=-beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal"
     primer_bind     complement(6309..6328)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     promoter        complement(10374..10392)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(10375..10392)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(10473..10492)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     10592..10614
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(10652..10670)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        10738..10842
                     /gene="bla"
                     /label=AmpR promoter
     CDS             10843..11703
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(11061..11080)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11874..12462
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12363..12382
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     12616..12633
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 taatacgact cactataggg agaccggcag atctgatccg ctcgagcgga tcatcgccgg
       61 gtgagtgtcc ccaccccacc ctgggcgcgc gggtcgttgg gacgccggga gacccccgcg
      121 tgggtcgagc gcgctgagtg tcccgtgcct ctttcctagg caaagagaag gagcccctgg
      181 agtcgcagta ccaggtgggc ccgctgttgg gcagcggtgg cttcggctcg gtctactctg
      241 gcatccgcgt cgccgacaac ttgccggtga gtgggcaacg ccgcctcggg gagggcgcgc
      301 ggagggatgc tttgagcagg agggtctgat ggagactcgg gggtcttctt ccaggtggcc
      361 attaagcacg tggagaagga ccggatttcc gattggggag aactggtgag tgagccggag
      421 ccagagaccc tctcatgggt gggtgggtgg cgacgggcgt gccggaaccg gcgcgctaac
      481 cctcgccctc actctgcagc ccaatggcac ccgagtgccc atggaagtgg tcctgttgaa
      541 gaaggtgagc tcgctcaaaa agcctgaact caccgcgacg tctgtcgaga agtttctgat
      601 cgaaaagttc gacagcgtct ccgacctgat gcagctctcg gagggcgaag aatctcgtgc
      661 tttcagcttc gatgtaggag ggcgtggata tgtcctgcgg gtaaatagct gcgccgatgg
      721 tttctacaaa gatcgttatg tttatcggca ctttgcatcg gccgcgctcc cgattccgga
      781 agtgcttgac attggggaat tcagcgagag cctgacctat tgcatctccc gccgtgcaca
      841 gggtgtcacg ttgcaagacc tgcctgaaac cgaactgccc gctgttctgc agccggtcgc
      901 ggaggccatg gatgcgatcg ctgcggccga tcttagccag acgagcgggt tcggcccatt
      961 cggaccgcaa ggaatcggtc aatacactac atggcgtgat ttcatatgcg cgattgctga
     1021 tccccatgtg tatcactggc aaactgtgat ggacgacacc gtcagtgcgt ccgtcgcgca
     1081 ggctctcgat gagctgatgc tttgggccga ggactgcccc gaagtccggc acctcgtgca
     1141 cgcggatttc ggctccaaca atgtcctgac ggacaatggc cgcataacag cggtcattga
     1201 ctggagcgag gcgatgttcg gggattccca atacgaggtc gccaacatct tcttctggag
     1261 gccgtggttg gcttgtatgg agcagcagac gcgctacttc gagcggaggc atccggagct
     1321 tgcaggatcg ccgcggctcc gggcgtatat gctccgcatt ggtcttgacc aactctatca
     1381 gagcttggtt gacggcaatt tcgatgatgc agcttgggcg cagggtcgat gcgacgcaat
     1441 cgtccgatcc ggagccggga ctgtcgggcg tacacaaatc gcccgcagaa gcgcggccgt
     1501 ctggaccgat ggctgtgtag aagtactcgc cgatagtgga aaccgacgcc ccagcactcg
     1561 tccgagggca aaggaatagt cgactacacg gactttgatg gtgagccagg ccccattatt
     1621 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
     1681 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctggg tcgacattga
     1741 ttattgacta gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg
     1801 gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc
     1861 cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat
     1921 tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat
     1981 catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat
     2041 gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc
     2101 gctattacca tggtcgaggt gagccccacg ttctgcttca ctctccccat ctcccccccc
     2161 tccccacccc caattttgta tttatttatt ttttaattat tttgtgcagc gatgggggcg
     2221 gggggggggg gggcgcgcgc caggcggggc ggggcggggc gaggggcggg gcggggcgag
     2281 gcggagaggt gcggcggcag ccaatcagag cggcgcgctc cgaaagtttc cttttatggc
     2341 gaggcggcgg cggcggcggc cctataaaaa gcgaagcgcg cggcgggcgg gagtcgctgc
     2401 gttgccttcg ccccgtgccc cgctccgcgc cgcctcgcgc cgcccgcccc ggctctgact
     2461 gaccgcgtta ctcccacagg tgagcgggcg ggacggccct tctcctccgg gctgtaatta
     2521 gcgcttggtt taatgacggc tcgtttcttt tctgtggctg cgtgaaagcc ttaaagggct
     2581 ccgggagggc cctttgtgcg ggggggagcg gctcgggggg tgcgtgcgtg tgtgtgtgcg
     2641 tggggagcgc cgcgtgcggc ccgcgctgcc cggcggctgt gagcgctgcg ggcgcggcgc
     2701 ggggctttgt gcgctccgcg tgtgcgcgag gggagcgcgg ccgggggcgg tgccccgcgg
     2761 tgcggggggg ctgcgagggg aacaaaggct gcgtgcgggg tgtgtgcgtg ggggggtgag
     2821 cagggggtgt gggcgcggcg gtcgggctgt aacccccccc tgcacccccc tccccgagtt
     2881 gctgagcacg gcccggcttc gggtgcgggg ctccgtgcgg ggcgtggcgc ggggctcgcc
     2941 gtgccgggcg gggggtggcg gcaggtgggg gtgccgggcg gggcggggcc gcctcgggcc
     3001 ggggagggct cgggggaggg gcgcggcggc cccggagcgc cggcggctgt cgaggcgcgg
     3061 cgagccgcag ccattgcctt ttatggtaat cgtgcgagag ggcgcaggga cttcctttgt
     3121 cccaaatctg gcggagccga aatctgggag gcgccgccgc accccctcta gcgggcgcgg
     3181 gcgaagcggt gcggcgccgg caggaaggaa atgggcgggg agggccttcg tgcgtcgccg
     3241 cgccgccgtc cccttctcca tctccagcct cggggctgcc gcagggggac ggctgccttc
     3301 gggggggacg gggcagggcg gggttcggct tctggcgtgt gaccggcggc tctagagcct
     3361 ctgctaacca tgttcatgcc ttcttctttt tcctacagct cctgggcaac gtgctggtta
     3421 ttgtgctgtc tcatcatttt ggcaaagaat tctagggata acagggtaat aattctaccg
     3481 ggtaggggag gcgcttttcc caaggcagtc tggagcatgc gctttagcag ccccgctggg
     3541 cacttggcgc tacacaagtg gcctctggcc tcacacattc cacatccacc ggtaggcgcc
     3601 aaccggctcc gttctttggt ggccccttcg cgccaccttc tactcctccc ctagtcagga
     3661 agttcccccc cgccccgcag ctcgcgtcgt gcaggacgtg acaaatggaa gtagcacgtc
     3721 tcactagtct cgtgcagatg gacagcaccg ctgagcaatg gaagcgggta ggcctttggg
     3781 gcagcggcca atagcagctt tgctccttcg ctttctgggc tcagaggctg ggaaggggtg
     3841 ggtccggggg cgggctcagg ggcgggctca ggggcggggc gggcgcccga aggtcctccg
     3901 gaggcccggc attctgcacg cttcaaaagc gcacgtctgc cgcgctgttc tcctcttcct
     3961 catctccggg cctttcgacc gatccagccg ccaccatgac cgagtacaag cccacggtgc
     4021 gcctcgccac ccgcgacgac gtcccccggg ccgtacgcac cctcgccgcc gcgttcgccg
     4081 actaccccgc cacgcgccac accgtcgacc cggaccgcca catcgagcgg gtcaccgagc
     4141 tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg
     4201 acggcgccgc ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg
     4261 ccgagatcgg cccgcgcatg gccgagttga gcggttcccg gctggccgcg cagcaacaga
     4321 tggaaggcct cctggcgccg caccggccca aggagcccgc gtggttcctg gccaccgtcg
     4381 gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg
     4441 aggcggccga gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc
     4501 ccttctacga gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc
     4561 gcacctggtg catgacccgc aagcccggtg cctgacgccc gccccacgac ccgcagcgcc
     4621 cgaccgaaag gagcgcacga ccccatggct ccgaccgaag ccacccgggg cggccccgcc
     4681 gaccccgcac ccgcccccga ggcccaccgc gggggacaca ccgaacacgc cgaccctgct
     4741 gaacacgcgg cgcagttcgg tgcccaggag cggatcgaaa ttgatgatct attaaacaat
     4801 aaagatgtcc actaaaatgg aagtttttcc tgtcatactt tgttaagaag ggtgagaaca
     4861 gagtacctac attttgaatg gaaggattgg agctacgggg gtgggggtgg ggtgggatta
     4921 gataaatgcc tgctctttac tgaaggctct ttactattgc tttatgataa tgtttcatag
     4981 ttggatatca taatttaaac aagcaaaacc aaattaaggg ccagctcatt cctcccactc
     5041 atgatctata gatctataga tctctcgtgg gatcattgtt tttctcttga ttcccacttt
     5101 gtggttctaa gtactgtggt ttccaaatgt gtcagtttca tagcctgaag aacgagatca
     5161 gcagcctctg ttccacatac acttcattct cagtattgtt ttgccaagtt ctaattccat
     5221 cagaagctta gggataacag ggtaatggat ccaccggtcg ccaccatggt gagcaagggc
     5281 gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc
     5341 cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg
     5401 aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg
     5461 acctacggcg tgcagtgctt cagccgctac cccgaccaca tgaagcagca cgacttcttc
     5521 aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc
     5581 aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag
     5641 ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac
     5701 tacaacagcc acaacgtcta tatcatggcc gacaagcaga agaacggcat caaggtgaac
     5761 ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag
     5821 aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcacccag
     5881 tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg
     5941 accgccgccg ggatcactct cggcatggac gagctgtaca agtaaagcgg ccgcgactct
     6001 agatcataat cagccatacc acatttgtag aggttttact tgctttaaaa aacctcccac
     6061 acctccccct gaacctgaaa cataaaatga atgcaattgt tgttgttaat tcactcctca
     6121 ggtgcaggct gcctatcaga aggtggtggc tggtgtggcc aatgccctgg ctcacaaata
     6181 ccactgagat ctttttccct ctgccaaaaa ttatggggac atcatgaagc cccttgagca
     6241 tctgacttct ggctaataaa ggaaatttat tttcattgca atagtgtgtt ggaatttttt
     6301 gtgtctctca ctcggaagga catatgggag ggcaaatcat ttaaaacatc agaatgagta
     6361 tttggtttag agtttggcaa catatgccat atgctggctg ccatgaacaa aggtggctat
     6421 aaagaggtca tcagtatatg aaacagcccc ctgctgtcca ttccttattc catagaaaag
     6481 ccttgacttg aggttagatt ttttttatat tttgttttgt gttatttttt tctttaacat
     6541 ccctaaaatt ttccttacat gttttactag ccagattttt cctcctctcc tgactactcc
     6601 cagtcatagc tgtccctctt ctcttatgaa gatccctcga cctgcagccc aagctggcgt
     6661 cgactacacg gactttgatg gtgagccagg cagcggccgc tagctgccca aggtgccggc
     6721 ctggaagcct ctggcggcct cggctccggc cctttgtaaa ggtcattggg ccgcctggct
     6781 cagagctggc aggggtgggg cagaggcgag cttcccggca tagtacagcc ggggatttcg
     6841 agctggttga acgaacctgc aacatttctg gcgtgattgg ggttttgttg ttgttgttgt
     6901 tgtttttgtt gttgctgctg gtgtttgctt gtttttcttt tcagcaggaa tttattaaat
     6961 tccatgcttt cccggtgaat aagaggtcgt gggtagccgg ctgtagccca gatttctaaa
     7021 gtatgaccca cttttccctc cagtaggagg tggagccgtt ggtggcggga gacattggtg
     7081 ctgatttttt tctttttctt tttctttttt tttttttttt cttttgtagt tttgttttgt
     7141 gggggagttt taaggtagtt cttagaactg cagggttttg ttttgttttg ttttgctttt
     7201 gagggtaaag gaataagagc cgcggtttca ccccacttgg gggtgttgtg gggtgaggag
     7261 gcctggaatg aatgttgtga aataagatct gttgctggtt tgaaaattag ttgggagtct
     7321 ccgctgggag gatgaaaacc tatctaccat agggaggggc ttggatcgcg ttctttcagc
     7381 agtggagggc aggaatagta gatcaaagcg cgagggtggg tcacgatccg agtggctcac
     7441 ccggacaaac tatggccttt caaaacttgt gactctggct tgtggtttga atttctttgg
     7501 aggatgccgg gctgggctgc gttttttgta tttaacagtt aatggctggc tgagtcctcc
     7561 cctgtgtttt ccccccctgg gcaagtcttt gctgccccct gttgagcacc acgaggctag
     7621 cacctttttt tttttttttt ttttttactc cccaaagttt gtaaacagga atcacgtggt
     7681 ctgaaaccag ccctgcaact gtacctttcc agtctaggga gtgggagagt gcagtgtcta
     7741 tgccttgctt gcatagaacc cggaggaaag cacaccctga gttagttgtg tataaaggcc
     7801 cttacctggg cccggtctag atgagactgg tgtggcaggg cctggggtcc ttgtagttaa
     7861 aggctaaccc actgagtggc ctctagactt gagcttttcg gtggctttat ttgtctttgg
     7921 gccgatgtgt aagggatttg ggtttagggt ttttgggctc ccagagagct accatatcat
     7981 ttcttcattt tctgtctgga ccagagaggc agagatggag agaaaaaaaa atatggttgt
     8041 tttaaagtca aatctgcctg tctgcctcct gagagctggg attaaaggca tgtgccacca
     8101 aacccgagcc aggcagtgtc tttattaaaa aatatttgaa aacctagtgg aggtggtggt
     8161 tcttgctttt aatccagccc ttgggaggta gaggtaggca gatctccgag ttcaaggcca
     8221 gcctggccta cagagtcact tccagagcta cacagagaaa ctttggagga gaaaaaaaaa
     8281 attaaaagaa gcggctcaga ctagctgctt tgttaacttt ttttttgcta atgctggttt
     8341 ctttctattc cctctgctgg tagggacccg agtgtacagt cctccagagt ggattcgcta
     8401 ccatcgctac cacggcaggt cggcagctgt ctggtccctt gggatcctgc tctatgacat
     8461 ggtctgcgga gatattccgt ttgagcacga tgaagagatc atcaagggcc aagtgttctt
     8521 caggcaaact gtctcttcag gtaacttccc tcagacaccc ccaatccttc ccaaaccctg
     8581 gttcagggtt ggtttgggga ttacaagagt gagagagtat tcctatccac ctttttattt
     8641 ttatttattc attttctttg ggggagagta aggccttagc ctgcctctga cccccaactg
     8701 ctgggactca agagatggtg ggttttcact gggtcagagt actccatttg ggctcaaatt
     8761 ggaacaagtt ttatatacat cccaccccaa cccccagacc ctgtaaaatc taagtaccag
     8821 cagagaccca gtctctaaac aaagtttaaa aaaaaaaaaa aaaaatgaag gttccggata
     8881 gtgtcagtgg aaggcgtgag gtaggtaggt gaattgaatc acctcatctt gctcactggt
     8941 ggctcatcaa aacccttgtc atccttccta tttctagtga gtgggtgtca tgggaaaggc
     9001 ccgagctatt gaagtctgaa accacaggtt taagagggga ggcacttttt taactgaaag
     9061 ctgattgctg gaggctttcg gtatccgttc tttcccaccg ggcagtgttt ggtataaaaa
     9121 gtaccgtgcc tctttaaaaa ccccagactt tgtaggtctg gggaagaaga cctttgcatt
     9181 acaaaaacaa gttgagtcat ctttaacctc tgtctgcccc gccttctgca gagtgtcagc
     9241 accttattaa atggtgcctg tccctgagac cgtcagatcg gccctccttt gaagaaatcc
     9301 ggaaccatcc gtggatgcag ggtgacctcc tgccccaggc agcttctgag atccatctgc
     9361 acagtctgtc accgggatcc agcaagtagc agcctttctg ctgctgtctg tcagaccctc
     9421 cagggaggga gagcttgtct tctggcctcc aacaggaccc tgcgtgacga tgcagggaca
     9481 gcaatgacaa ctcattccag actccaggtc cctggaggag cctcccacaa gggaaagaga
     9541 ctacttcact ggtcctgggc ccctctttgc gcgccccgcc cccagactca gcgtctagtg
     9601 ttgctgggct cccctgaatc catccctggt tgggggtggg agtggatcag agccctgtcg
     9661 atggaacttt agtcaccatg gagactgggt caccaagatg ggccgggggg gagaggaaaa
     9721 cagtctgggg ggtgggataa aaactagcac catatttaag tccctgtcac ctcttccaac
     9781 tcctgagtgc cttctgtggg gactccgcct gtgctgggag aaatacttga actttacctc
     9841 ttacctgctg cttctcaaac tctgcctggg ttttgcttcc ttcctctcct ctcctttccc
     9901 atcccccact tcctatgaaa ggtgccatgg gagaggctac agggccaact gctgagccac
     9961 ctgccccttt tctgtctcct ttagtaaaat tcccaagcaa actggtcttc ctctttggtt
    10021 tcgacttaac tgtttcaaaa ccaagacctc acacagaacg cacaaccaat gcaaaacaaa
    10081 aaacaaaaaa aacaaaaaaa aaaccagaca agctgtaaat gtgtgtatag ttccggcatg
    10141 gtagcctgca aaaggactgt agatgataac aattttaaag aagaaaaaaa aatgccttta
    10201 agttatttta tgtgttgggg gtttggtttg gttttttgtt tttgtttttt tttttctttt
    10261 ttattctttt ttgcttgttt ttgaaagatg gcacgttcta gcccggaagt tttaatgtat
    10321 ttatttattt atttatttgg ttgccttcct gtccaagctt cagctgctcg agttctatag
    10381 tgtcacctaa atcgtatgtg tatgatacat aaggttatgt attaattgta gccgcgttct
    10441 aacgacaata tgtccatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta
    10501 agccagcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg
    10561 gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca
    10621 ccgtcatcac cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt tttataggtt
    10681 aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc
    10741 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
    10801 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc
    10861 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
    10921 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa
    10981 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg
    11041 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa
    11101 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
    11161 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
    11221 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta
    11281 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
    11341 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca
    11401 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
    11461 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc
    11521 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca
    11581 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca
    11641 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
    11701 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa
    11761 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
    11821 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
    11881 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
    11941 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
    12001 gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac
    12061 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
    12121 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
    12181 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
    12241 gaactgagat acctacagcg tgagcattga gaaagcgcca cgcttcccga agggagaaag
    12301 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
    12361 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
    12421 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
    12481 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
    12541 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc
    12601 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa
    12661 ccgcctctcc ccgcgcgttg gccgattcat taatgcaggt taacctggct tatcgaaat
//