pIMP1-Padc

LOCUS       pIMP1-Padc                4945 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Plasmid with adc(CA_P0165) promoter for gene expression in 
            Clostridium acetobutylicum ATCC 824 and Clostridium beijerinckii 
            NCIMB8052.
ACCESSION   .
VERSION     .
KEYWORDS    pIMP1-Padc
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4945)
  AUTHORS   Zhang N, Shao L, Jiang Y, Gu Y, Li Q, Liu J, Jiang W, Yang S
  TITLE     I-SceI-mediated scarless gene modification via allelic exchange in 
            Clostridium.
  JOURNAL   J Microbiol Methods. 2015 Jan;108:49-60. doi: 
            10.1016/j.mimet.2014.11.004. Epub 2014 Nov 15.
  PUBMED    25451462
REFERENCE   2  (bases 1 to 4945)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..4945
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      complement(166..754)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(246..265)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(925..1785)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1548..1567
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1786..1890)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     1958..1976
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(2014..2036)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2136..2155
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2349..2371
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2363..2380
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2364..2380
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4709..4725)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4709..4725)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4722..4744)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4733..4749
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4757..4787)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4802..4823
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4940..12)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
       61 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
      121 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
      181 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
      241 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
      301 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
      361 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
      421 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
      481 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
      541 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
      601 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
      661 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
      721 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
      781 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
      841 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
      901 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
      961 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
     1021 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
     1081 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
     1141 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
     1201 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
     1261 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
     1321 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
     1381 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
     1441 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
     1501 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
     1561 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
     1621 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
     1681 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
     1741 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
     1801 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
     1861 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt
     1921 ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt
     1981 tcgtctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac
     2041 ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc
     2101 gggtgttggc gggtgtcggg gctggcttaa ctatgcggca tcagagcaga ttgtactgag
     2161 agtgcaccat atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag
     2221 gcgccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc
     2281 gctattacgc cagctggcga aagggggatg tgctgcaagg cgattaagtt gggtaacgcc
     2341 agggttttcc cagtcacgac gttgtaaaac gacggccagt gaattcgagc tcggtacccg
     2401 gggatcctct agagtcgaca aaagtcacct tcctaaattt aataatgttt agcttttcta
     2461 acatacttta tcttcacatt ataaatcgcc tctagtccta tttatttata tttacattat
     2521 aacacatatt gtttttttaa gtaaattttt acaccatata ttaatttttt ctactagctt
     2581 tcgaaaataa aagtacactt cctaaaaatt ctgcataata gatttatata aacttatgtg
     2641 gaggtgtttt tactgcagcc aagcttaatc gcatttcata gattgacctc ccaataacta
     2701 cgtggtgtta ttgggaggtc aatctatttc atttgcctct tgctcaaagt tcccaaattc
     2761 gagtaagagg tatttttgtt tttggtcgtc gcctctcatt agtagttcag ggtttaacat
     2821 taatactcca gtttttcttt ttataatatt tccttcttct aagattttaa gtgttgttat
     2881 tactgtttgt agacttgttc ctgtagcttt tgctatttct cttgttgtag ctatcattgt
     2941 attgttactt aagtggacat tatctaggat atagttaacg attttaagtt tttttccgcc
     3001 aatcatatct aacatactta ttaattgcac tatatatgcc tttacgaagt taccagacgt
     3061 ttgtttacgg tataacttgt ctacctctat gacttctcca ctttcttcgt ctatgagcct
     3121 ctgagagcct ttatagactg ttccatatct ttctttcatc tttttctcac tccttatttt
     3181 aaactattct aactatatca taactgttct aaaaaaaaaa gaacatttgt taaaagaaat
     3241 tagaacaaaa tgagtgaaaa attagaacaa acaaattcct tataaacctt atcatctcaa
     3301 cctatattaa gattttacct agttgaatct tcttttctat ataaagcgtc ggagcatatc
     3361 agggggttat ctaacgtaaa tgctaccctt cggctcgctt tcgctcggca ttgacgtcag
     3421 atactgcacc ccctgaaccc ccatgctcca acagcaaaaa ggaaactttt tgctgctttt
     3481 ccgacgctta ttcgcttcgc tcatatttat atagaaaaga agtgaatgcg caaaagacat
     3541 aatcgattca caaaaaatag gtacacgaaa aacaagttaa gggatgcagt ttatgcatcc
     3601 cttaacttac ttattaaata atttatagct attgaaaaga gataagaatt gttcaaagct
     3661 aatattgttt aaatcgtcaa ttcctgcatg ttttaaggaa ttgttaaatt gattttttgt
     3721 aaatattttc ttgtattctt tgttaaccca tttcataacg aaataattat acttctgttt
     3781 atctttgtgt gatattcttg atttttttct atttaatctg ataagtgagc tattcacttt
     3841 aggtttagga tgaaaatatt ctcttggaac catacttaat atagaaatat caacttctgc
     3901 cattaaaaat aatgccaatg agcgttttgt atttaataat cttttagcaa acccgtattc
     3961 cacgattaaa taaatctcat cagctatact atcaaaaaca attttgcgta ttatatccgt
     4021 acttatgtta taaggtatat taccaaatat tttataggat tggtttttag gaaatttaaa
     4081 ctgcaatata tccttgttta aaacttggaa attatcgtga tcaacaagtt tattttctgt
     4141 agttttgcat aatttatggt ctatttcaat ggcagttacg aaattacacc tctgtactaa
     4201 ttcaagggta aaatgccctt ttcctgagcc gatttcaaag atattatcat gttcatttaa
     4261 tcttatattt gtcattattt tatctatatt atgttttgaa gtaataaagt tttgactgtg
     4321 ttttatattt ttctcgttca ttataaccct ctttattttt tcctccttat aaaattagta
     4381 taattatagc acgagctctg ataaatatga acatgatgag tgatcgttaa atttatactg
     4441 caatctgatg cgattattga ataaaagata tgagagattt atctagtttc tttttttaca
     4501 agaaaaaaga aagttcttaa aggttttata cttttggtcg tagagcacac ggtttaacga
     4561 cttaattacg aagtaaataa gtctagtgtg ttagacttta atgttttttt aaggcattag
     4621 tgcatttaag cgtcagagca tggctttatg ccgagaaaac tattggttgg aatggcgtgt
     4681 gtgttagcca aagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc
     4741 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct
     4801 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
     4861 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
     4921 ttgggcgctc ttccgcttcc tcgct
//