| 质粒名称: | pSF11-wmBaoJin |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0000449 |
| 产品用途: | To express wmBaoJin in neurons of worms |
| 存储实验室: | KIRYL PIATKEVICH Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pSF11 |
| 表达系统: | 线虫表达 |
| 启动子: |
tag-168
|
| 拷贝数: | 中拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | TOP10 |
|
插入基因信息 |
|
| 插入基因名称: | wmBaoJin |
| 物种来源: | worm |
| 密码子优化: | 优化 |
|
文献引用方法 |
|
| 参考文献部分: |
Bright and stable monomeric green fluorescent protein derived from StayGold
Zhang H., Lesnov G.D., Subach O.M., Zhang W., Kuzmicheva T.P., Vlaskina A.V., Samygina V.R., Chen L., Ye X., Nikolaeva A.Y., Gabdulkhakov A., Papadaki S., Qin W., Borshchevskiy V., Perfilov M.M., Gavrikov A.S., Drobizhev M., Mishin A.S., Piatkevich K.D., Subach F.V.
Nat. Methods 2024 214 21, 657–665 (2024)
|
买家导航
pSF11-wmBaoJin载体质粒基本信息
pSF11-wmBaoJin质粒图谱载体图谱和pSF11-wmBaoJin载体序列质粒序列多克隆位点信息
pSF11-wmBaoJin质粒载体简介
pSF11-wmBaoJin质粒序列载体序列
LOCUS pSF11-wmBaoJin 5625 bp DNA circular SYN 07-APR-2024
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5625)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Apr 23, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..5625
/mol_type="other DNA"
/organism="synthetic DNA construct"
misc_feature 54..1441
/label=tag-168 promoter
primer_bind 1449..1471
/label=pSF11-F
primer_bind 1520..1540
/label=pSF11-Fw-Seq
misc_feature 1558..1561
/label=optimized sequence for ribosomal binding site
misc_feature 1558..1561
/label=codon adapted insert for smGFP-myc
/note="codon adapted by
https://worm.mpi-cbg.de/codons/cgi-bin/optimize.py
Intron * 2 (3 requested, but 1 is inside myc regions and
thus removed)
CAI set to 0.60
Check all 3 boxes
"
CDS 1562..2368
/codon_start=1
/label=wmBaoJin
/translation="MVSKGEEENMASTPFKFQLKGTINGKSFTVEGEGEGNSHEGSHKG
KYVCTSGKLPMSWAALGTTFGYGMKYYTKYPSGLKVSLNIYILTNPDYLNFQNWFREVM
PGGFTYDRHIQYKGDGSIHAKHQHFMKNGTYHNIVEFTGQDFKVSLNSSVLTNHTYLNF
QENSPVLTGDMNVSLPNEVPQIPRDDGVECPVTLLYPLLSDKSKYVEAHQYTICKPLHN
QPAPDVPYHWIRKQYTQSKDDAEERDHICQSETLEAHLKGMDELYK"
misc_feature 1802..1852
/label=intron-1
misc_feature 2003..2053
/label=intron-2
primer_bind complement(2410..2426)
/label=pSF11-Rv-Seq
primer_bind complement(2412..2433)
/label=pSF11-R
promoter 3504..3608
/gene="bla"
/label=AmpR promoter
CDS 3609..4469
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
CDS 3807..4466
/label=AmpR
rep_origin 4564..5246
/label=ColE1 origin
rep_origin 4640..5228
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 5307..5333
/label=WKG-304-F1
primer_bind complement(5325..5343)
/label=0507-619-R
protein_bind 5516..5537
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind 5549..5571
/label=lac-R
promoter 5552..5582
/label=lac promoter
/note="promoter for the E. coli lac operon"
misc_binding 5586..5608
/label=LacO
protein_bind 5590..5606
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind join(5614..5625,1..5)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
ORIGIN
1 atgaccatga ttacgccaag cttgcatgcg cggccgcact gactgggccg gccggatcca
61 taggtatgcc tgtcattctc tatttttaaa cgttcctgca gcagtagcac aacagagcca
121 gcagttttcc tcttcttctc catcttcttc tgcttcttcc tctcttccca aaaaaaaaaa
181 aaaaaaaaaa gtgagggggc tcactgcccc cccccccctc ccccccccct cttctccttg
241 aagctcatcc agacgtcgct gtcctcctcc tcctcgtccc cggggcgctg tgtgtgtgtg
301 tgtgtgctcc gtgagcatgt agcagcttca gcccatttaa aggcgcacac tctctgcatc
361 tctgcgtgtc tcgacactcg ttttttctct tcttttctgc tgcacacaca cacacacaca
421 cacacacgct catgccagag cacttcttgc tccggggggc tccgcgcgga gcccgagagg
481 acctcctcct cctttttctt cttcgctttt tagttcttct tgcctcggtc tcgccccttg
541 ttttgctctc catgtagtat atacatctgg gtgtgtgtgt gtgtgtgcac ctttggagca
601 ttttttgaac ttttccagaa ctttggagca acattttcag cttgttcagt gcacttttta
661 ggctgaaaat cctattttta ggttttcgaa agtttttttg gagcattttt cgtgcaattt
721 aggtgcaaaa ttaaaaaaaa gggttttgta gtgaattaaa atttttttga aactttcgcc
781 tttttaaaaa aatgtttcta atatctttaa aaattggaat attttggaaa acaatttgtt
841 ttcaattggc aatttattaa ttctgagctt ctgagcgtaa attttttttt attttggagc
901 aattctgaga tgtcttagtg catttttcgg gcatacataa aaaaaaattt atcttaataa
961 taaaaacctt aaatttaggc ttcaccgtct ggaaattcaa ttttttcagc tgaaaaactt
1021 tcaaaaaaaa aaaaattaaa ttttcacatt tattttctct ggaattttgg agcaatttca
1081 aagctgtctt agtgcatttt tagtacacaa atattttttc atttttctta aattttaata
1141 atctaagctt aaataaaggc ttcaccgtct gaaaatgcaa cttttttcag atgaaatatt
1201 aaaaaaaaaa acctctttta ttaattttgt gctccaaact ttcccacggt acgcaaatca
1261 tgtaatatgt acttttaaac attatttttc caatcaaaaa tatccaacag cttttcctta
1321 caatttgaaa atttttatta aaatttcaaa aaaatttttt tttttttcac ccgtggattt
1381 ccctccaaca agttacccca aaaataaaaa taaaaattcc tgcaggcgcc caccgggatc
1441 cggcgcgcct ctagaggatc cccgggattg gccaaaggac ccaaaggtat gtttcgaatg
1501 atactaacat aacatagaac attttcagga ggacccttgg ctagcgtcga cggtaccaaa
1561 aatggtatcc aagggagaag aagaaaacat ggctagtacc ccattcaagt tccagcttaa
1621 gggaactatt aacggaaagt ctttcactgt tgaaggagaa ggagaaggaa actctcacga
1681 aggatctcat aagggaaagt acgtttgcac ttctggaaag cttccaatgt cttgggctgc
1741 tcttggaact actttcggat atggaatgaa gtattacact aagtacccat ctggacttaa
1801 ggtaagttta aacatatata tactaactaa ccctgattat ttaaattttc agaactggtt
1861 ccgcgaagtt atgccaggag gattcactta tgatcgccat atccagtaca agggagatgg
1921 atctatccat gctaagcatc agcatttcat gaagaatgga acttaccaca acatcgttga
1981 attcactgga caagatttca aggtaagttt aaacagttcg gtactaacta accatacata
2041 tttaaatttt caggaaaact ccccagttct taccggagat atgaacgttt cccttccaaa
2101 cgaagttcca caaatcccac gtgatgatgg agttgagtgc ccagttaccc ttctttaccc
2161 acttctttcc gataagtcca agtacgttga ggctcatcag tacactatct gcaagccact
2221 tcataaccaa ccagctccag atgttccata ccattggatc cgcaagcaat acactcaatc
2281 taaggatgat gctgaggagc gcgatcacat ctgccaatct gagactcttg aggctcacct
2341 taagggaatg gatgagcttt acaagtaaga gctccgcatc ggccgctgtc atcagatcgc
2401 catctcgcgc ccgtgcctct gacttctaag tccaattact cttcaacatc cctacatgct
2461 ctttctccct gtgctcccac cccctatttt tgttattatc aaaaaaactt cttcttaatt
2521 tctttgtttt ttagcttctt ttaagtcacc tctaacaatg aaattgtgta gattcaaaaa
2581 tagaattaat tcgtaataaa aagtcgaaaa aaattgtgct ccctcccccc attaataata
2641 attctatccc aaaatctaca caatgttctg tgtacacttc ttatgttttt tttacttctg
2701 ataaattttt tttgaaacat catagaaaaa accgcacaca aaatacctta tcatatgtta
2761 cgtttcagtt tatgaccgca atttttattt cttcgcacgt ctgggcctct catgacgtca
2821 aatcatgctc atcgtgaaaa agttttggag tatttttgga atttttcaat caagtgaaag
2881 tttatgaaat taattttcct gcttttgctt tttgggggtt tcccctattg tttgtcaaga
2941 gtttcgagga cggcgttttt cttgctaaaa tcacaagtat tgatgagcac gatgcaagaa
3001 agatcggaag aaggtttggg tttgaggctc agtggaaggt gagtagaagt tgataatttg
3061 aaagtggagt agtgtctatg gggtttttgc cttaaatgac agaatacatt cccaatatac
3121 caaacataac tgtttcctac tagtcggccg tacgggccct ttcgtctcgc gcgtttcggt
3181 gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa
3241 gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg
3301 ggctggctta actatgcggc atcagagcag attgtactga gagtgcacca tatgcggtgt
3361 gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcggcctta attaagggcc
3421 tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag
3481 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
3541 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
3601 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
3661 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
3721 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
3781 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
3841 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
3901 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
3961 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
4021 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
4081 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
4141 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
4201 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
4261 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
4321 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
4381 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
4441 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
4501 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
4561 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
4621 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
4681 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
4741 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc
4801 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
4861 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
4921 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
4981 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag cattgagaaa
5041 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
5101 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
5161 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
5221 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
5281 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
5341 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
5401 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
5461 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
5521 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt
5581 tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagct
//
