pCMV-hA3A-BE3-Y130F

LOCUS       pCMV-hA3A-BE3-Y130F                8442 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Expresses hA3A-BE3-Y130F in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    pCMV-hA3A-BE3-Y130F
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8442)
  AUTHORS   Wang X, Li J, Wang Y, Yang V, Wei J, Wu J, Wang R, Huang X, Chen J, 
            Yang L
  TITLE     Efficient base editing in methylated regions with a human 
            APOBEC3A-Cas9 fusion.
  JOURNAL   Nat Biotechnol.,  2018
REFERENCE   2  (bases 1 to 8442)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..8442
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             complement(968..1564)
                     /codon_start=1
                     /gene="human APOBEC3A"
                     /gene_synonym="A3A"
                     /product="human cytidine deaminase (C to U editing enzyme) 
                     that efficiently edits methylated cytosines (Wang et al., 
                     2018)"
                     /label=APOBEC3A
                     /note="also known as hA3A"
                     /translation="MEASPASGPRHLMDPHIFTSNFNNGIGRHKTYLCYEVERLDNGTS
                     VKMDQHRGFLHNQAKNLLCGFYGRHAELRFLDLVPSLQLDPAQIYRVTWFISWSPCFSW
                     GCAGEVRAFLQENTHVRLRIFAARIFDYDPLYKEALQMLRDAGAQVSIMTYDEFKHCWD
                     TFVDHQGCPFQPWDGLDEHSQALSGRLRAILQNQGN"
     regulatory      1561..1570
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     primer_bind     complement(1577..1596)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1578..1596)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(1638..1841)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(1667..1687)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        1842..2221
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     2393..2412
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        2491..2595
                     /gene="bla"
                     /label=AmpR promoter
     CDS             2596..3456
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2814..2833)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3627..4215
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4116..4135
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4369..4386
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4503..4524
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        4539..4569
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4577..4593
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4582..4604
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4601..4617
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4601..4617
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    4688..4912
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     4901..4918
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     CDS             complement(4941..4958)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(4967..4987)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(5000..5248)
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             complement(5261..919)
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(D10A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the D10A mutation in the RuvC catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
ORIGIN
        1 ctctcagtat gtcagatagg aggattgcat cgctaaggtt tttggcagcc aaaaataagt
       61 ccgcatactg atctccaatt tgtgccagta gattgtcgag atcgtcatcg tacgtgtcct
      121 tactaagctg caatttggca tcttcagcta agtcgaagtt cgacttaaaa tttggtgtca
      181 ggcctagtga gagcgctata aggttaccga acaacccatt tttcttctct ccgggtaatt
      241 gtgcgatcag gttttctagc cgtcgggatt tagagaggcg ggcgctaaga atagccttcg
      301 catccacgcc acttgcattt atagggttct cttcaaacaa ctgattatag gtttgtacta
      361 actggatgaa cagtttgtcg acatccgagt tgtccggatt tagatcaccc tcaatgagaa
      421 agtgcccacg gaactttatc atatgggcaa gagccaagta gattaacctc aggtccgctt
      481 tatcagttga gtcaactagc ttttttctga ggtgataaat cgttgggtac ttttcatgat
      541 atgccacctc atctactatg tttccaaaga tggggtgccg ttcatgtttc ttgtcctctt
      601 cgacaaggaa ggactcttcc aaacggtgaa agaaagaatc gtcaactttg gccatctcat
      661 tgctaaaaat ttcttgtaag taacatattc ggttcttgcg acgtgtatac cttctccgag
      721 cggttcgttt caggcgagtc gcctctgccg tttcgccact atcgaatagg agggcaccga
      781 taagattctt tttaatcgaa tgacggtctg tgttccccaa caccttaaat ttctttgaag
      841 gtactttgta ttcatcggtt atgacagccc atccaacgga attagtgccg atggctaaac
      901 caatagaata ctttttatca ctttcgggtg tggcggactc tgaggtcccg ggagtctcgc
      961 tgccgctgtt tccctgattc tggagaatgg cccgcagcct cccactcagg gcttggctgt
     1021 gctcatctag tccatcccag ggctggaagg gacatccctg gtggtccaca aaggtgtccc
     1081 agcagtgctt aaattcatcg taggtcatga tggagacttg ggccccagca tcccgcagca
     1141 tttgcagtgc ctccttatat agggggtcgt aatcaaagat gcgggcagcg aagatacgca
     1201 gtctcacgtg tgtgttctcc tgaaggaacg cacgcacttc cccggcacag ccccaggaga
     1261 agcaggggct ccaggagatg aaccaagtga ccctgtagat ctgggccggg tccaactgca
     1321 aagaaggaac caggtccaag aagcgcagct ccgcatggcg gccgtaaaag ccacagagaa
     1381 gattcttagc ctggttgtgt agaaagcccc tgtgctggtc catcttgacc gaggtgccat
     1441 tgtccaggcg ctccacttcg tagcacaggt aggtcttatg ccttccaatg ccattgttaa
     1501 agttggaagt gaatatgtgt ggatccatca agtgtctggg cccggatgct gggctggctt
     1561 ccatggtggc ggctctccct atagtgagtc gtattagcgg ccgcggatct ctagcggatc
     1621 tgacggttca ctaaaccagc tctgcttata tagacctccc accgtacacg cctaccgccc
     1681 atttgcgtca atggggcgga gttgttacga cattttggaa agtcccgttg attttggtgc
     1741 caaaacaaac tcccattgac gtcaatgggg tggagacttg gaaatccccg tgagtcaaac
     1801 cgctatccac gcccattgat gtactgccaa aaccgcatca ccatggtaat agcgatgact
     1861 aatacgtaga tgtactgcca agtaggaaag tcccataagg tcatgtactg ggcataatgc
     1921 caggcgggcc atttaccgtc attgacgtca atagggggcg tacttggcat atgatacact
     1981 tgatgtactg ccaagtgggc agtttaccgt aaatactcca cccattgacg tcaatggaaa
     2041 gtccctattg gcgttactat gggaacatac gtcattattg acgtcaatgg gcgggggtcg
     2101 ttgggcggtc agccaggcgg gccatttacc gtaagttatg taacgcggaa ctccatatat
     2161 gggctatgaa ctaatgaccc cgtaattgat tactattaat aactagtcaa taatcaatgt
     2221 caacgcgtat atctggcccg tacatcgcga agcagcgcaa aacgcctaac cctaagcaga
     2281 ttcttcatgc aattgtcggt caagccttgc cttgttgtag cttaaatttt gctcgcgcac
     2341 tactcagcga cctccaacac acaagcaggg agcagatact ggcttaacta tgcggcatca
     2401 gagcagattg tactgagagt cgaccctagg ggatcgggag atcgatctcc cgatccgtcg
     2461 acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
     2521 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
     2581 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
     2641 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
     2701 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
     2761 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
     2821 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat
     2881 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
     2941 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
     3001 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
     3061 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
     3121 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa
     3181 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
     3241 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
     3301 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
     3361 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
     3421 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
     3481 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
     3541 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     3601 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     3661 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     3721 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
     3781 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     3841 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     3901 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     3961 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     4021 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     4081 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     4141 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     4201 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     4261 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
     4321 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg
     4381 agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt
     4441 cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca
     4501 attaatgtga gttagctcac tcattaggca ccctaggctt tacactttat gcttccggct
     4561 cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat
     4621 gattacgcca agctctagct agaggtcgac ggtatcgagc cccagctggt tctttccgcc
     4681 tcagaagcca tagagcccac cgcatcccca gcatgcctgc tattgtcttc ccaatcctcc
     4741 cccttgctgt cctgccccac cccacccccc agaatagaat gacacctact cagacaatgc
     4801 gatgcaattt cctcatttta ttaggaaagg acagtgggag tggcaccttc cagggtcaag
     4861 gaaggcacgg gggaggggca aacaacagat ggctggcaac tagaaggcac agtcgaggct
     4921 gatcagcggg tttaaactca atggtgatgg tgatgatgac cggttagact ttcctcttct
     4981 tcttgggaga accaccagag agcatcttaa tcttgttctc accgttgcta tcctgtatga
     5041 ccagagccca aggcttgtat tcaggggcgt cgctagtcag aagcatgaca ttctcgtcgg
     5101 tgctctcgtc gtaggcggtg tgcacgagta tatcgctttc cggcttgttc ccaatgactt
     5161 cttccacctc ctctgggagc atgaggatgg attcctggat aaccagttgc ttaccggtct
     5221 ccttttcaat aatatctgac agattagtag aaccaccaga gtcaccccca agctgtgaca
     5281 aatctatccg agtttcatat aatcccgtga tggattggtg aatcagtgtc gcgtctagca
     5341 cctccttggt agaagtgtat cgtttgcgat ctatcgttgt gtcaaaatac ttgaatgcgg
     5401 ctggagcgcc gaggttggta agagtaaaca aatggataat attttccgcc tgctcacgta
     5461 tgggtttatc cctgtgcttg ttgtatgcgc ttaatacttt gtccagattg gcatcagcta
     5521 ggatgactct cttactgaat tccgaaattt gctctatgat ttcgtcgaga taatgtttgt
     5581 gctgctcaac aaaaagttgc ttctgttcgt tatcttcagg tgaacctttc aacttctcgt
     5641 aatgggacgc taaatacagg aaattcacgt atttagacgg tagtgcgagt tcgttcccct
     5701 tttgaagctc tccggcgcta gccaacatcc gttttcggcc attttctaac tcaaacagac
     5761 tatactttgg tagtttaatt atgagatcct tttttacttc cttgtaacct ttcgcctcaa
     5821 ggaagtcgat ggggttcttt tcaaaagacg agcgctccat aatcgttatc cccaataatt
     5881 ctttgactga cttcagtttc ttggattttc ccttctcaac ttttgccact actaggacag
     5941 aataggcaac tgtagggcta tcgaagccac cgtacttttt cgggtcccag tcctttttac
     6001 gagcgatgag cttatcacta ttcctttttg gaagaatcga ttcctttgaa aaccctccgg
     6061 tctgcacctc agttttcttt actatgttga cttggggcat ggacaaaact tttctcaccg
     6121 tcgcgaagtc ccggccctta tcccatacga tttcacctgt ctccccattg gtttcaatta
     6181 aaggtcgttt gcgtatctct ccgtttgcca gagtgatttc cgtcttaaag aaattcataa
     6241 tgttagaata aaagaagtat ttggctgtag ccttgcctat ctcctgttcg cttttcgcga
     6301 tcatcttacg gacgtcataa actttgtaat caccatacac aaactcactt tctagcttcg
     6361 ggtatttctt aatgagtgcg gtccctacga cggcattaag ataagcgtcg tgcgcatggt
     6421 ggtagttatt tatctcccta actttataga attgaaaatc ctttctgaag tccgacacca
     6481 attttgactt taaagtgatt actttgactt cccgaatcag cttatcgttc tcgtcgtatt
     6541 tcgtattcat tcgggaatct agtatctgtg caacatgctt tgtgatttgg cgggtttcca
     6601 cgagctgacg tttaataaat ccggccttgt caagttcaga caagccaccc ctctcagctt
     6661 tagttaagtt atcgaacttt ctttgcgtta tcagtttcgc atttaggagc tgccgccaat
     6721 agttcttcat tttctttacg acttcctcgc ttggaacatt gtcacttttc cctcggttct
     6781 tatccgagcg tgtaagcact ttattgtcga ttgaatcgtc cttcaaaaag gattggggta
     6841 caatgtgatc gacgtcgtaa tcagataaac ggtttatgtc cagttcctga tcaacataca
     6901 tgtcccttcc attttgtagg taatagaggt aaagtttctc gttctgcaat tgggtatttt
     6961 ccacaggatg ctcctttaag atctggctgc ccagttcttt aataccctct tctattctct
     7021 tcatccgctc tcgactgttt ttttgcccct tctgagtcgt ttgattttcg cgtgccatct
     7081 cgattacaat gttttccggt ttgtgacgtc ccatgacctt aactagctca tccactactt
     7141 tgactgtctg gagtatgccc tttttgatgg ctggcgaacc agcaagattc gcaatatgtt
     7201 cgtgcaatga gtccccttgt ccggaaacct gtgccttttg tatatcctct ttgaaggtta
     7261 aagagtcatc atggatcagc tgcataaagt tcctattggc gaagccgtcg ctctttagaa
     7321 aatcgagaat agttttacca ctttgcttgt ctcttatccc gttgataagt ttccgcgaca
     7381 atcgtcccca gcccgtatag cgacgcctct ttaactgttt cataacctta tcgtcgaaca
     7441 ggtgagcgta tgtttttagt ctttcctcaa tcatttcccg atcttcaaag agggtaagag
     7501 tcaacactat atcttctaag atatcttcat tctcttcgtt atccaggaag tccttatctt
     7561 taattatctt taggaggtca tgatacgtac caagtgacgc attaaatcga tcttctaccc
     7621 cggagatctc gacagaatcg aagcattcaa ttttcttaaa gtagtcctct ttcaattgct
     7681 taactgtcac tttgcggttg gtcttgaata acagatctac tattgctttc ttctgttctc
     7741 cgcttagaaa ggcgggttta cgcatgccct cagtgacata cttaactttc gtgagttcat
     7801 tgtacactgt gaaatactcg taaagtaaac tgtgcttagg caatactttt tcgttcggta
     7861 aattcttgtc aaagttggtc atcctctcga tgaacgattg agctgacgca cctttatcga
     7921 caacttcctc aaaattccat ggagtaatcg tttcttcgga ctttcttgtc atccatgcga
     7981 accgagagtt ccctcgggcc aggggtccca catagtaagg tatgcgaaag gttaggattt
     8041 tctcaatctt ttcacgattg tctttgagga acggataaaa atcctcctgc cttctaagta
     8101 tagcatgcaa ttcgcctaag tggatttgat gtggaatgct accgttgtcg aaagtccgct
     8161 gctttcgcag tagatcttcg cgattgagtt ttacaagcaa ctcttccgtc ccatccatct
     8221 tctctaatat gggtttgata aacttgtaga attcctcttg actcgctccg ccgtcaatat
     8281 aacctgcgta cccgtttttc gactgatcaa agaatatttc cttatatttc tcaggcagtt
     8341 gctgacggac tagggccttg agaagtgtca agtcttggtg atgttcatcg taccttttga
     8401 tcattgaagc ggataacggc gccttggtaa tctcagtatt aa
//