| 质粒名称: | pSL1199 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0002423 |
| 产品用途: | Mammalian PhCMV-driven constitutive expression vector for a LIRP variant with the LOV2 domain inserted between amino acids 38 and 39 of MCP (PhCMV-MCP(LOV2 38/39)-pA). |
| 存储实验室: | Mingqi XIE Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pcDNA3.1 |
| 表达系统: | 哺乳动物表达 |
| 启动子: | PhCMV |
| 拷贝数: | 未拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | Stbl3 |
|
插入基因信息 |
|
| 插入基因名称: | MCP(LOV2 38/39) |
| 物种来源: | synthetic |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
Rationally designed light-inducible RNA-releasing protein for translational regulation and optogenetic control of gene therapies
NA
NA
|
pSL1199
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pSL1199载体质粒基本信息
pSL1199质粒图谱载体图谱和pSL1199载体序列质粒序列多克隆位点信息
pSL1199质粒载体简介
pSL1199质粒序列载体序列
LOCUS pSL1199 6213 bp DNA circular SYN 05-MAR-2026
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6213)
AUTHORS Trial User
TITLE Direct Submission
JOURNAL Exported Aug 28, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6213
/mol_type="other DNA"
/organism="synthetic DNA construct"
misc_feature 232..819
/label=CMV
enhancer 235..614
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 615..818
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 863..882
/label=T7
promoter 863..881
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind 880..923
/label=oSCL2210
primer_bind 886..918
/label=Oscl32-1
primer_bind 886..918
/label=oSCL1118
primer_bind 901..918
/label=Oscl42-1
regulatory 901..910
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
regulatory 902..910
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
CDS 910..1023
/codon_start=1
/product="bacteriophage MS2 coat protein"
/label=MS2-N55K
/note="binds with high affinity to a specific stem-loop
structure in the viral RNA (Lim et al., 1994)"
/translation="ASNFTQFVLVDNGGTGDVTVAPSNFANGVAEWISSNSR"
primer_bind complement(1004..1043)
/label=oSCL2215
misc_feature 1024..1437
/label=LOV2
primer_bind 1024..1044
/label=oSCL1757
primer_bind complement(1024..1043)
/label=oSCL2217
primer_bind complement(1024..1043)
/label=oSCL2219
primer_bind complement(1416..1437)
/label=oSCL1758
primer_bind 1418..1509
/label=oSCL2218
primer_bind 1418..1457
/label=oSCL2216
primer_bind 1418..1437
/label=oSCL2220
CDS 1438..1710
/codon_start=1
/product="bacteriophage MS2 coat protein"
/label=MS2-N55K
/note="binds with high affinity to a specific stem-loop
structure in the viral RNA (Lim et al., 1994)"
/translation="SQAYKVTCSVRQSSAQKRKYTIKVEVPKVATQTVGGVELPVAAWR
SYLNMELTIPIFATNSDCELIVKAMQGLLKDGNPIPSAIAANSGIY"
misc_feature 1438
/label=Feature 16
primer_bind complement(1465..1482)
/label=oSCL2217
misc_feature 1482^1483
/label=Feature 17
primer_bind complement(1584..1605)
/label=oSCL2219
primer_bind 1605..1625
/label=oSCL2220
misc_feature 1605^1606
/label=Feature 18
primer_bind complement(1690..1733)
/label=oSCL2211
primer_bind complement(1690..1719)
/label=Oscl32-2
primer_bind complement(1690..1710)
/label=oSCL093
terminator 1810..2037
/label=bGH PA
polyA_signal 1813..2037
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
rep_origin 2083..2511
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 2525..2854
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 2705..2840
/label=SV40 ori
/note="SV40 origin of replication"
misc_feature 2705..2782
/label=SV40 ORI
CDS 2921..3715
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
CDS 2924..3715
/label=Kan/neoR
polyA_signal 3889..4010
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(4055..4075)
/label=M13-rev
primer_bind complement(4059..4075)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
misc_binding complement(4081..4103)
/label=LacO
protein_bind 4083..4099
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(4107..4137)
/label=lac promoter
/note="promoter for the E. coli lac operon"
promoter complement(4108..4137)
/label=lac
protein_bind 4152..4173
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
rep_origin complement(4461..5046)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(5217..6077)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
CDS complement(5220..5879)
/label=AmpR
promoter complement(6078..6182)
/gene="bla"
/label=AmpR promoter
promoter complement(6119..6147)
/label=Amp prom
ORIGIN
1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
901 gccaccatgg cttcaaactt tactcagttc gtgctcgtgg acaatggtgg gacaggggat
961 gtgacagtgg ctccttctaa tttcgctaat ggggtggcag agtggatcag ctccaactca
1021 cggggtttgg aacgtatcga aaagaatttt gtcatcacgg acccgcgtct gcccgacaat
1081 ccgattatct tcgcgtcaga ctctttctta caactgactg agtatagtag agaggagata
1141 ttggggcgta actgtagatt tcttcagggg ccagaaactg atcgggctac cgttcgcaag
1201 atacgtgacg caatagacaa ccagaccgag gtgacggtgc agctgattaa ctacacaaag
1261 tctgggaaga agttctggaa cctgtttcat ttacaaccta tgagggacca aaaaggtgac
1321 gttcaatatt tcatcggggt tcagttagat gggactgagc acgtgagaga tgcagcagaa
1381 agagagggtg taatgcttat taaaaaaaca gccgagaata tcgacgaagc cgctggtagc
1441 caggcctaca aggtgacatg cagcgtcagg cagtctagtg cccagaagag aaagtatacc
1501 atcaaggtgg aggtccccaa agtggctacc cagacagtgg gcggagtcga actgcctgtc
1561 gccgcttgga ggtcctacct gaacatggag ctcactatcc caattttcgc taccaattct
1621 gactgtgaac tcatcgtgaa ggcaatgcag gggctcctca aagacggtaa tcctatccct
1681 tccgccatcg ccgctaactc aggtatctac taaggatcca ctagtccagt gtggtggaat
1741 tctgcagata tccagcacag tggcggccgc tcgagtctag agggcccgtt taaacccgct
1801 gatcagcctc gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc
1861 cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg
1921 catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca
1981 agggggagga ttgggaagac aatagcaggc atgctgggga tgcggtgggc tctatggctt
2041 ctgaggcgga aagaaccagc tggggctcta gggggtatcc ccacgcgccc tgtagcggcg
2101 cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc
2161 tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc
2221 gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg
2281 accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg
2341 tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg
2401 gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt
2461 cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat taattctgtg
2521 gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
2581 aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg
2641 cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc
2701 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
2761 tttttttatt tatgcagagg ccgaggccgc ctctgcctct gagctattcc agaagtagtg
2821 aggaggcttt tttggaggcc taggcttttg caaaaagctc ccgggagctt gtatatccat
2881 tttcggatct gatcaagaga caggatgagg atcgtttcgc atgattgaac aagatggatt
2941 gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact gggcacaaca
3001 gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct
3061 ttttgtcaag accgacctgt ccggtgccct gaatgaactg caggacgagg cagcgcggct
3121 atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc
3181 gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct
3241 tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga
3301 tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg
3361 gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc
3421 agccgaactg ttcgccaggc tcaaggcgcg catgcccgac ggcgaggatc tcgtcgtgac
3481 ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat
3541 cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg ctacccgtga
3601 tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc
3661 cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctgagcggg
3721 actctggggt tcgaaatgac cgaccaagcg acgcccaacc tgccatcacg agatttcgat
3781 tccaccgccg ccttctatga aaggttgggc ttcggaatcg ttttccggga cgccggctgg
3841 atgatcctcc agcgcgggga tctcatgctg gagttcttcg cccaccccaa cttgtttatt
3901 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
3961 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgt
4021 ataccgtcga cctctagcta gagcttggcg taatcatggt catagctgtt tcctgtgtga
4081 aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc
4141 tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc
4201 cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc
4261 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
4321 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
4381 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
4441 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
4501 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
4561 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
4621 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
4681 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
4741 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
4801 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
4861 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc
4921 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
4981 accaccgctg gtagcggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
5041 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
5101 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
5161 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
5221 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
5281 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
5341 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
5401 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
5461 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
5521 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
5581 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
5641 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
5701 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
5761 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
5821 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
5881 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
5941 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
6001 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
6061 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
6121 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
6181 cgcacatttc cccgaaaagt gccacctgac gtc
//
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