| 质粒名称: | GW1-Peredox-mCherry |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 32380 |
| 存储实验室: | Gary Yellen |
|
载体骨架基本信息 |
|
| 载体名称: | GW1 |
| 载体出品公司: | British Biotechnology |
| 载体抗性: | 氨苄霉素 |
| 空载体大小: | 5300 bp |
| 载体类型: | 哺乳细胞表达 |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | Peredox-mCherry |
| 插入大小: | 2800 bp |
| 启动子 | CMV |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | PspOMI(未破坏) |
| 3' 克隆位点: | SalI(未破坏) |
| 5' 测序引物 | CMV-F |
| 3' 测序引物 | SV40pA-R |
|
文献引用方法 |
|
| 材料和方法部分: | GW1-Peredox-mCherry was a gift from Gary Yellen (Addgene plasmid # 32380) |
| 参考文献部分: | Imaging Cytosolic NADH-NAD(+) Redox State with a Genetically Encoded Fluorescent Biosensor. Hung YP, Albeck JG, Tantama M, Yellen G. Cell Metab. 2011 Oct 5;14(4):545-54. 10.1016/j.cmet.2011.08.012 PubMed 21982714 |
买家导航

GW1-Peredox-mCherry载体质粒基本信息
GW1-Peredox-mCherry质粒图谱载体图谱和GW1-Peredox-mCherry载体序列质粒序列多克隆位点信息

GW1-Peredox-mCherry质粒载体简介
GW1-Peredox-mCherry质粒序列载体序列
LOCUS GW1-Peredox-mCherry 8045 bp DNA SYN
DEFINITION GW1-Peredox-mCherry
ACCESSION
KEYWORDS
SOURCE
ORGANISM other sequences; artificial sequences; vectors.
FEATURES Location/Qualifiers
source 1..8045
/organism="GW1-Peredox-mCherry"
/mol_type="other DNA"
CDS complement(316..1281)
/label="ORF frame 3"
CDS 640..3408
/label="ORF frame 1"
CDS complement(1453..2004)
/label="ORF frame 3"
CDS complement(2202..2765)
/label="ORF frame 1"
CDS complement(2497..3429)
/label="ORF frame 3"
gene 2701..3405
/label="mCherry"
/gene="mCherry"
misc_feature 2878..2931
/label="Q66M (mCherry)"
terminator 3533..3664
/label="SV40_PA_terminator"
misc_feature 3621..3640
/label="EBV_rev_primer"
promoter complement(3684..3702)
/label="M13_reverse_primer"
misc_feature complement(3701..3723)
/label="M13_pUC_rev_primer"
promoter complement(3737..3766)
/label="lac_promoter"
rep_origin complement(4075..4694)
/label="pBR322_origin"
gene complement(4849..5709)
/label="Ampicillin"
/gene="Ampicillin"
CDS complement(4849..5709)
/label="ORF frame 3"
promoter complement(5788..5816)
/label="AmpR_promoter"
misc_feature complement(5975..5997)
/label="pGEX_3_primer"
misc_feature complement(6184..6203)
/label="SV40pro_F_primer"
misc_feature complement(6234..6377)
/label="lacZ_a"
CDS complement(6234..6761)
/label="ORF frame 1"
misc_feature 6348..6370
/label="M13_pUC_fwd_primer"
promoter 6363..6379
/label="M13_forward20_primer"
promoter 6929..7505
/label="CMV_immearly_promoter"
misc_feature 7008..7295
/label="CAG_enhancer"
misc_feature 7462..7482
/label="CMV_fwd_primer"
promoter 7463..7532
/label="CMV_promoter"
promoter 7475..7594
/label="CMV2_promoter"
misc_feature 7506..7525
/label="pCEP_fwd_primer"
misc_feature 7508..7532
/label="LNCX_primer"
CDS complement(8000..413)
/label="ORF frame 1"
ORIGIN
1 ggacatgggc tcttctccgg tagcggcgga gcttctacat ccgagccctg ctcccatgcc
61 tccagcgact catggtcgct cggcagctcc ttgctcctaa cagtggaggc cagacttagg
121 cacagcacga tgcccaccac caccagtgtg ccgcacaagg ccgtggcggt agggtatgtg
181 tctgaaaatg agctcgggga gcgggcttgc accgctgacg catttggaag acttaaggca
241 gcggcagaag aagatgcagg cagctgagtt gttgtgttct gataagagtc agaggtaact
301 cccgttgcgg tgctgttaac ggtggagggc agtgtagtct gagcagtact cgttgctgcc
361 gcgcgcgcca ccagacataa tagctgacag actaacagac tgttcctttc catgggtctt
421 ttctgcagtc accgtccttg acacgaagct tcccggccac agcaatcgat ccatgacaca
481 accagtgaca agcagtcccc cagtgaaacc gcatccgcat ccgagtccga taccgataaa
541 gattctgaat cggagtgagt gccgcgtccg agagcgttcc ctgtccacgt ccaccatcgg
601 cggagcaggt gtgcctgagg cctctagatt gggcccacca tggttgctag caaagttcct
661 gaagcagcca tttccagact gattacttac ctgagaattc tggaagagct ggaagcccaa
721 ggtgtacacc gcaccgcctc cgagcaactg ggcgagctgg cccaggtcac cgccttccag
781 gttgataagg acctgtccta cttcggctcc tacggcactg acggcgttgg ctacactgta
841 ccggtcctca agagagaact gcgccatatc ctcggtctga accgcaaatg gggcctgtgc
901 atcgtgggga tgggccgcct gggatccgct cttgctgatt ggcctggttt tggcgagagc
961 ttcgagctgc gcggtttctt cgatgttgac ccaggtatgg ttggtcggcc ggttcgcggt
1021 ggtgttatcg aacacgttga tctgctgccc cagcgcgtac ctggtcgcat cgaaatcgct
1081 ctgctgacgg ttccacgcga ggcagcacag aaggctgccg acctgctggt tgccgctggc
1141 atcaagggca tcctgaactt cgctccagtt gtactggagg ttcccaagga agtggctgtt
1201 gagaacgttg acatcctggc cggtctgacg cgtctgagct ttgccattct gaaccccacg
1261 tggagcgcag caggtgggca tggtttcact gctcacaacg tgtatatcat ggctgacaaa
1321 cagaagaacg gtatcaaggc caacttcaag atccgccaca acatcgaaga cggcggcgtt
1381 cagctggctg atcactacca gcagaacacc ccaatcggcg acggccctgt tctgctgcct
1441 gacaaccact acttaagcat ccagtctaaa ctgtccaaag acccaaacga gaagcgcgac
1501 cacatggttc tgctggaatt tgttaccgcc gctggcatca cccacggcat ggacgaactg
1561 tacaagggcg gcaccggggg atctatggtt tccaaaggcg aggaactgtt cactggtgtt
1621 gttcctatcc tggttgaact ggacggcgac gttaacggcc acaaattcag cgttagcggg
1681 gaaggcgaag gagacgctac ctacggcaag ctgactctga agttcatctg caccactggc
1741 aagctgcctg ttccttggcc taccctggtt actaccttct cctatggtgt tatggttttc
1801 gccagatatc ctgaccacat gaagcagcac gacttcttca agagcgccat gcctgagggc
1861 tacgttcagg aacgcaccat ctttttcaag gacgacggca actataagac acgcgctgag
1921 gttaagttcg agggcgacac tctggttaac cgcatcgagc tgaagggcat cgacttcaag
1981 gaggacggca acatcctggg ccataagctt gaatataaca ccaaggtacc tgaagccgcc
2041 atcagccgcc tcatcactta cctccgcatc ctggaggagc tcgaagccca aggcgttcac
2101 cgcactgcaa gcgagcagct gggcgaactg gcacaggtta cagcattcca ggttgacgaa
2161 gatctgtcct atttcggttc ctacggcacc gacggtgttg gctacacagt ccctgtcctg
2221 aagcgcgagc tgcgccacat cctgggcctg aaccgcaagt ggggcttatg catcgttggc
2281 atgggccgcc tcggtagtgc actggcagac tggccgggct ttggtgaatc tttcgaactg
2341 cgcggcttct tcgacgttga tccaggcatg gttgggcgcc ctgttcgcgg tggcgttatc
2401 gagcatgttg acctcctgcc acagcgcgtc ccgggtcgca tcgagatcgc cctgctgacc
2461 gttcctcgcg aagctgcaca gaaggcagcc gacttactag tcgccgctgg tatcaaggga
2521 attctgaact tcgcccctgt tgttctggag gttcctaagg aagttgctgt tgaaaacgtt
2581 gatttcctgg ccggcctgac ccgcctgagc ttcgcaatcc tgaatcctaa gtggcgcgaa
2641 gaaatgatgg gttccggaac tgggggcaac gcctctgacg gtggtgggtc tggtggtatg
2701 gtgagcaagg gcgaggagga taacatggcc atcatcaagg agttcatgcg cttcaaggtg
2761 cacatggagg gctccgtgaa cggccacgag ttcgagatcg agggcgaggg cgagggccgc
2821 ccctacgagg gcacccagac cgccaagctg aaggtgacca agggtggccc cctgcccttc
2881 gcctgggaca tcctgtcccc tcagttcatg tacggctcca aggcctacgt gaagcacccc
2941 gccgacatcc ccgactactt gaagctgtcc ttccccgagg gcttcaagtg ggagcgcgtg
3001 atgaacttcg aggacggcgg cgtggtgacc gtgacccagg actcctccct gcaggacggc
3061 gagttcatct acaaggtgaa gctgcgcggc accaacttcc cctccgacgg ccccgtaatg
3121 cagaagaaga ccatgggctg ggaggcctcc tccgagcgga tgtaccccga ggacggcgcc
3181 ctgaagggcg agatcaagca gaggctgaag ctgaaggacg gcggccacta cgacgctgag
3241 gtcaagacca cctacaaggc caagaagccc gtgcagctgc ccggcgccta caacgtcaac
3301 atcaagttgg acatcacctc ccacaacgag gactacacca tcgtggaaca gtacgaacgc
3361 gccgagggcc gccactccac cggcggcatg gacgaactgt acaagtgagt cgacgaattc
3421 attgatcata atcagccata ccacatttgt agaggtttta cttgctttaa aaaacctccc
3481 acacctcccc ctgaacctga aacataaaat gaatgcaatt gttgttgtta acttgtttat
3541 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
3601 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
3661 gatccgtacc gagctcgcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc
3721 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
3781 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
3841 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
3901 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
3961 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
4021 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
4081 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
4141 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
4201 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
4261 ttcgggaagc gtggcgcttt ctcaatgctc acgctgtagg tatctcagtt cggtgtaggt
4321 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
4381 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
4441 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
4501 gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa
4561 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
4621 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
4681 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
4741 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
4801 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
4861 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
4921 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
4981 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
5041 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
5101 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
5161 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
5221 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
5281 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
5341 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
5401 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
5461 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
5521 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
5581 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
5641 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
5701 aatactcata ctcttccttt ttcaataagc ggccgcggcc atgccggcca ctagtctcga
5761 gttattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
5821 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg
5881 tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct
5941 ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga
6001 cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag
6061 cgggtgttgg cgggtgtcgg ggctggctta actatgcggc atcagagcag attgtactga
6121 gagtgcacca tatgaagacg tcgcctcctc actacttctg gaatagctca gaggccgagg
6181 cggcctcggc ctctgcataa ataaaaaaaa ttagtcagcg cgccattcgc cattcaggct
6241 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa
6301 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg
6361 ttgtaaaacg acggccagtg ccatctgcag tgaataataa aatgtgtgtt tgtccgaaat
6421 acgcgtttga gatttctgtc ccgactaaat tcatgtcgcg cgatagtggt gtttatcgcc
6481 gatagagatg gcgatattgg aaaaatcgat atttgaaaat atggcatatt gaaaatgtcg
6541 ccgatgtgag tttctgtgta actgatatcg ccatttttcc aaaagttgat ttttgggcat
6601 acgcgatatc tggcgatacg cttatatcgt ttacggggga tggcgataga cgcctttggt
6661 gacttgggcg attctgtgtg tcgcaaatat cgcagtttcg atataggtga cagacgatat
6721 gaggctatat cgccgataga ggcgacatca agctggcaca tggccaatgc atatcgatct
6781 atacattgaa tcaatattgg ccattagcca tattattcat tggttatata gcataaatca
6841 atattggcta ttggccattg catacgttgt atccatatca taatatgtac atttatattg
6901 gctcatgtcc aacattaccg ccatgttgac attgattatt gactagttat taatagtaat
6961 caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg
7021 taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt
7081 atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac
7141 ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg
7201 acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact
7261 ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt
7321 ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc
7381 ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc
7441 gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata
7501 taagcagagc tcgtttagtg aaccgtcaga tcgcctggag acgccatcca cgctgttttg
7561 acctccatag aagacaccgg gaccgatcca gcctccgcgg ccgggaacgg tgcattggaa
7621 cgcggattcc ccgtgccaag agtgacgtaa gtaccgccta tagagtctat aggcccaccc
7681 ccttggcttc ttatgcatgc tatactgttt ttggcttggg gtctatacac ccccgcttcc
7741 tcatgttata ggtgatggta tagcttagcc tataggtgtg ggttattgac cattattgac
7801 cactccccta ttggtgacga tactttccat tactaatcca taacatggct ctttgccaca
7861 actctcttta ttggctatat gccaatacac tgtccttcag agactgacac ggactctgta
7921 tttttacagg atggggtctc atttattatt tacaaattca catatacaac accaccgtcc
7981 ccagtgcccg cagtttttat taaacataac gtgggatctc cacgcgaatc tcgggtacgt
8041 gttcc
//
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