pmNeonGreen-NUP98

LOCUS       pmNeonGreen-NUP98         7500 bp DNA     circular SYN 21-NOV-2023
DEFINITION  Cloning vector pEGFP-C2 with enhanced green fluorescent protein 
            gene, complete sequence.
ACCESSION   U57606
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7500)
  AUTHORS   Cormack BP, Valdivia RH, Falkow S.
  TITLE     FACS-optimized mutants of the green fluorescent protein (GFP)
  JOURNAL   Gene 173 (1 SPEC NO), 33-38 (1996)
  PUBMED    8707053
REFERENCE   2  (bases 1 to 7500)
  AUTHORS   Kitts PA.
  TITLE     pEGFP-C2 complete sequence
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 7500)
  AUTHORS   Kitts PA.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-1996) P.A. Kitts, CLONTECH Laboratories, Inc., 
            1020 East Meadow Circle, Palo Alto, CA 94303-4230, USA
REFERENCE   4  (bases 1 to 7500)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 5, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Gene 173 (1
            SPEC NO), 33-38 (1996)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 3; type: "Journal Article"; journalName: "Submitted 
            (06-MAY-1996) P.A. Kitts, CLONTECH Laboratories, Inc., 1020 East 
            Meadow Circle, Palo Alto, CA 94303-4230, USA"
COMMENT     This vector can be obtained from CLONTECH Laboratories, Inc., 1020 
            East Meadow Circle, Palo Alto, CA 94303-4230, USA. To place an order
            call (415) 424-8222 or (800) 662-2566, extension 1. International 
            customers, please contact your local distributor. For technical 
            information, call (415) 424-8222 or (800) 662-2566, extension 3.
            This sequence has been compiled from information in the sequence 
            databases, published literature and other sources, together with 
            partial sequences obtained by CLONTECH. If you suspect there is an 
            error in this sequence, please contact CLONTECH's Technical Service 
            Department at (415) 424-8222 or (800) 662-2566, extension 3 or 
            E-mail TECH@CLONTECH.COM.
FEATURES             Location/Qualifiers
     source          1..7500
                     /mol_type="other DNA"
                     /db_xref="taxon:47840"
                     /organism="Cloning vector pEGFP-C2"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     580..632
                     /label=0830-1043-F
     regulatory      607..616
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             613..1320
                     /codon_start=1
                     /product="bright monomeric yellow-green fluorescent protein
                     derived from LanYFP (Shaner et al., 2013)"
                     /label=mNeonGreen
                     /translation="MVSKGEEDNMASLPATHELHIFGSINGVDFDMVGQGTGNPNDGYE
                     ELNLKSTKGDLQFSPWILVPHIGYGFHQYLPYPDGMSPFQAAMVDGSGYQVHRTMQFED
                     GASLTVNYRYTYEGSHIKGEAQVKGTGFPADGPVMTNSLTAADWCRSKKTYPNDKTIIS
                     TFKWSYTTGNGKRYRSTARTTYTFAKPMAANYLKNQPMYVFRKTELKHSKTELNFKEWQ
                     KAFTDVMGMDELYK"
     misc_feature    629..634
                     /label=SDS
     regulatory      868..877
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(1300..1338)
                     /label=0830-1043-R
     CDS             1345..4155
                     /codon_start=1
                     /label=NUP98 and 96
                     /translation="MFNKSFGTPFGGGTGGFGTTSTFGQNTGFGTTSGGAFGTSAFGSS
                     NNTGGLFGNSQTKPGGLFGTSSFSQPATSTSTGFGFGTSTGTANTLFGTASTGTSLFSS
                     QNNAFAQNKPTGFGNFGTSTSSGGLFGTTNTTSNPFGSTSGSLFGPSSFTAAPTGTTIK
                     FNPPTGTDTMVKAGVSTNISTKHQCITAMKEYESKSLEELRLEDYQANRKGPQNQVGAG
                     TTTGLFGSSPATSSATGLFSSSTTNSGFAYGQNKTAFGTSTTGFGTNPGGLFGQQNQQT
                     TSLFSKPFGQATTTQNTGFSFGNTSTIGQPSTNTMGLFGVTQASQPGGLFGTATNTSTG
                     TAFGTGTGLFGQTNTGFGAVGSTLFGNNKLTTFGSSTTSAPSFGTTSGGLFGNKPTLTL
                     GTNTNTSNFGFGTNTSGNSIFGSKPAPGTLGTGLGAGFGTALGAGQASLFGNNQPKIGG
                     PLGTGAFGAPGFNTTTATLGFGAPQAPVALTDPNASAAQQAVLQQHINSLTYSPFGDSP
                     LFRNPMSDPKKKEERLKPTNPAAQKALTTPTHYKLTPRPATRVRPKALQTTGTAKSHLF
                     DGLDDDEPSLANGAFMPKKSIKKLVLKNLNNSNLFSPVNRDSENLASPSEYPENGERFS
                     FLSKPVDENHQQDGDEDSLVSHFYTNPIAKPIPQTPESAGNKHSNSNSVDDTIVALNMR
                     AALRNGLEGSSEETSFHDESLQDDREEIENNSYHMHPAGIILTKVGYYTIPSMDDLAKI
                     TNEKGECIVSDFTIGRKGYGSIYFEGDVNLTNLNLDDIVHIRRKEVVVYLDDNQKPPVG
                     EGLNRKAEVTLDGVWPTDKTSRCLIKSPDRLADINYEGRLEAVSRKQGAQFKEYRPETG
                     SWVFKVSHFSKYGLQDSDEEEEEHPSKTSTKKLKTAPLPPASQTTPLQMALNGKPAPPP
                     QVEKKGQ"
     misc_feature    1521..1526
                     /label=SDS
     primer_bind     1640..1656
                     /label=MCS-F
     primer_bind     2002..2021
                     /label=NUP98-Fseq
     primer_bind     2440..2465
                     /label=MCS-F2
     polyA_signal    4288..4409
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(4416..4871)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        4898..5002
                     /gene="bla"
                     /label=AmpR promoter
     promoter        5004..5361
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             5396..6190
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     polyA_signal    6422..6469
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      6798..7386
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggtcgcca ccatggtgag caagggcgag gaggataaca tggcctctct cccagcgaca
      661 catgagttac acatctttgg ctccatcaac ggtgtggact ttgacatggt gggtcagggc
      721 accggcaatc caaatgatgg ttatgaggag ttaaacctga agtccaccaa gggtgacctc
      781 cagttctccc cctggattct ggtccctcat atcgggtatg gcttccatca gtacctgccc
      841 taccctgacg ggatgtcgcc tttccaggcc gccatggtag atggctccgg ctaccaagtc
      901 catcgcacaa tgcagtttga agatggtgcc tcccttactg ttaactaccg ctacacctac
      961 gagggaagcc acatcaaagg agaggcccag gtgaagggga ctggtttccc tgctgacggt
     1021 cctgtgatga ccaactcgct gaccgctgcg gactggtgca ggtcgaagaa gacttacccc
     1081 aacgacaaaa ccatcatcag tacctttaag tggagttaca ccacgggaaa tggcaagcgc
     1141 taccggagca ctgcgcggac cacctacacc tttgccaagc caatggcggc taactatctg
     1201 aagaaccagc cgatgtacgt gttccgtaag acggagctca agcactccaa gaccgagctc
     1261 aacttcaagg agtggcaaaa ggcctttacc gatgtgatgg gcatggacga gctgtacaag
     1321 tccggccgga ctcagatctc gagcatgttt aacaaatcat ttggaacacc ctttgggggt
     1381 ggcacaggtg gctttggcac aacttcaaca tttggacaga atactggctt tggcactact
     1441 agtggagggg catttggaac atctgcattt ggttctagca acaatactgg aggcctcttt
     1501 ggaaattcac agactaaacc aggaggattg tttggaacca gttcatttag ccagccagct
     1561 acctccacaa gcactggctt tgggtttggt acgtcaacag gaacagcaaa taccttgttt
     1621 ggaactgcaa gcacagggac cagtctcttc tcatcccaaa acaatgcctt tgcacaaaat
     1681 aaaccaactg gctttggcaa ttttggaacc agtactagca gtggaggact ctttggaacc
     1741 acaaatacca cctctaatcc ttttggcagc acatctggct ccctctttgg gccaagtagt
     1801 tttacagctg ctcctactgg gactactatt aaatttaacc ctccaactgg tacagatact
     1861 atggtcaaag ctggagttag cactaacata agtaccaagc accagtgtat tactgctatg
     1921 aaagaatatg aaagcaagtc actagaggaa cttcgtttag aggattatca ggctaacagg
     1981 aagggcccac agaaccaggt gggagcaggt accacaactg gcttgtttgg gtcttctcca
     2041 gccacttcca gcgcaacagg actcttcagc tcctccacca ctaattcagg ctttgcatat
     2101 ggtcagaaca aaactgcctt tggaactagt acaactggat ttggaacaaa tccaggtggt
     2161 ctctttggcc aacagaatca gcagactacc agcctcttca gcaaaccatt tggccaggct
     2221 acaaccaccc agaacactgg cttttccttt ggtaatacca gcaccatagg acagccaagc
     2281 accaacacca tgggattatt tggagtaacc caagcctcac agcctggagg tctttttggg
     2341 acagctacaa acaccagcac tgggacagca tttggaacag gaacaggtct ctttgggcag
     2401 accaatactg gatttggtgc tgttggttcg accctgtttg gcaataacaa gcttactaca
     2461 tttggaagca gcacaaccag tgcaccttca tttggtacaa ccagtggcgg gctctttggt
     2521 aacaaaccaa ccctgacttt aggaaccaat acaaacactt ctaattttgg ttttggcaca
     2581 aataccagtg ggaatagtat ttttggaagt aaaccagcac ctgggactct tggaactggg
     2641 cttggtgcag gatttggaac agctcttggt gctggacagg catctttgtt tgggaacaac
     2701 caacctaaga ttggagggcc tcttggtaca ggagcctttg gggcccctgg atttaatact
     2761 acgacagcca ctttgggctt tggagccccc caggccccag tagctttgac agatccaaat
     2821 gcttctgctg cccagcaggc tgttctccag cagcacatca atagtctaac atactcacct
     2881 tttggagact ctcctctctt ccggaatccg atgtcagacc ctaagaagaa ggaagagaga
     2941 ttgaaaccaa caaatccagc agcccagaag gctcttacta cacctactca ttataaactg
     3001 acaccccgcc ctgccactag agtccggcca aaggctttac aaacaacagg cacagccaag
     3061 tcacatctct ttgatgggct ggatgacgat gaaccatccc tagccaatgg agcattcatg
     3121 cccaagaaga gcattaagaa gttggttttg aagaacctta ataatagcaa tctcttttct
     3181 cctgttaatc gtgattcaga aaatctagct tcaccatctg aatatccaga aaatggagag
     3241 agatttagtt tcctaagcaa acctgttgat gagaatcacc agcaggatgg agatgaagat
     3301 tcccttgttt cacattttta tactaaccct attgccaaac ctattcctca aaccccagaa
     3361 agtgctggaa ataaacacag caacagcaac agtgtggatg ataccattgt tgcattaaac
     3421 atgcgtgctg ctttgcgaaa tgggctggaa ggaagcagtg aagaaacgtc ttttcatgat
     3481 gagtcacttc aggatgaccg agaagaaata gaaaataatt cttaccatat gcacccagca
     3541 ggtattattc tcactaaggt tggttactat actattccat ctatggatga ccttgctaaa
     3601 attaccaatg aaaaaggaga gtgcattgtc tctgatttca ctattggtcg gaaaggttat
     3661 ggttcaatct attttgaagg agatgtgaat ttgacaaatc taaatttgga tgatattgtg
     3721 catatccgga ggaaagaagt agttgtctac ttagatgata accaaaaacc acctgtgggt
     3781 gaagggctaa ataggaaggc tgaagttaca ttggatggag tttggccaac agataaaaca
     3841 tctcgttgtt taataaagag cccagatcgc cttgctgata tcaactatga aggaagattg
     3901 gaagcagttt caaggaaaca gggagctcaa ttcaaagaat accggcctga aactggttct
     3961 tgggtgttta aggtctccca tttttctaag tatggccttc aggattctga tgaagaggag
     4021 gaggagcatc cgtctaaaac tagtacaaag aagttgaaga ctgctccttt gcctcctgca
     4081 agccagacta cgcccttgca gatggctctt aatggcaaac ctgcacctcc acctcaggta
     4141 gagaaaaaag gacagtgagg atccaccgga tctagataac tgatcataat cagccatacc
     4201 acatttgtag aggttttact tgctttaaaa aacctcccac acctccccct gaacctgaaa
     4261 cataaaatga atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa
     4321 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
     4381 ggtttgtcca aactcatcaa tgtatcttaa cgcgtaaatt gtaagcgtta atattttgtt
     4441 aaaattcgcg ttaaattttt gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg
     4501 caaaatccct tataaatcaa aagaatagac cgagataggg ttgagtgttg ttccagtttg
     4561 gaacaagagt ccactattaa agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta
     4621 tcagggcgat ggcccactac gtgaaccatc accctaatca agttttttgg ggtcgaggtg
     4681 ccgtaaagca ctaaatcgga accctaaagg gagcccccga tttagagctt gacggggaaa
     4741 gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct
     4801 ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta atgcgccgct
     4861 acagggcgcg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
     4921 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
     4981 ataatattga aaaaggaaga gtcctgaggc ggaaagaacc agctgtggaa tgtgtgtcag
     5041 ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
     5101 aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
     5161 agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc
     5221 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat
     5281 gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt
     5341 ggaggcctag gcttttgcaa agatcgatca agagacagga tgaggatcgt ttcgcatgat
     5401 tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta
     5461 tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca
     5521 ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcaaga
     5581 cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga
     5641 cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct
     5701 cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg
     5761 gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga
     5821 gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca
     5881 tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgagcatgc ccgacggcga
     5941 ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg
     6001 cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc
     6061 gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt
     6121 gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga
     6181 gttcttctga gcgggactct ggggttcgaa atgaccgacc aagcgacgcc caacctgcca
     6241 tcacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc
     6301 cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac
     6361 cctaggggga ggctaactga aacacggaag gagacaatac cggaaggaac ccgcgctatg
     6421 acggcaataa aaagacagaa taaaacgcac ggtgttgggt cgtttgttca taaacgcggg
     6481 gttcggtccc agggctggca ctctgtcgat accccaccga gaccccattg gggccaatac
     6541 gcccgcgttt cttccttttc cccaccccac cccccaagtt cgggtgaagg cccagggctc
     6601 gcagccaacg tcggggcggc aggccctgcc atagcctcag gttactcata tatactttag
     6661 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
     6721 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
     6781 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     6841 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     6901 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg
     6961 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     7021 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     7081 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     7141 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     7201 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     7261 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     7321 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     7381 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     7441 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgccatgcat
//