| 质粒名称: | pU6-DNMT1-PegRNA-H1-Nicking sgRNA |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0002210 |
| 产品用途: | Simultaneously express pegRNA and nicking sgRNA targeting the DNMT1 gene |
| 存储实验室: | Zhuobin Liang Lab 梁卓斌课题组 |
|
载体骨架基本信息 |
|
| 载体名称: |
pU6
|
| 表达系统: | 哺乳动物表达 |
| 启动子: | U6, H1 |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | DNMT1-pegRNA-nicking-sgRNA |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
TRU-PE: A Universal, Trackable Prime Editor Toolkit for Robust Single-and Multi-Locus Genome Engineering
Zhichao Qiu, Keke Sun, Qingwei Zeng, Ziwei Luo, Xinran Liu, Yaping Li, Xiang Lei, Ruilin Zhao, Zhen Zhang, Damia Romero-Moya, Yuan Yang, Xuan Wang, Ehsan Hashemi, Joe Z. Zhang, Xiaolong Wang, Alessandra Giorgetti, Zhuobin Liang
bioRxiv 2026.02.20.706938
|
pU6-DNMT1-PegRNA-H1-Nicking sgRNA
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pU6-DNMT1-PegRNA-H1-Nicking sgRNA载体质粒基本信息
pU6-DNMT1-PegRNA-H1-Nicking sgRNA质粒图谱载体图谱和pU6-DNMT1-PegRNA-H1-Nicking sgRNA载体序列质粒序列多克隆位点信息
pU6-DNMT1-PegRNA-H1-Nicking sgRNA质粒载体简介
pU6-DNMT1-PegRNA-H1-Nicking sgRNA质粒序列载体序列
LOCUS pU6-DNMT1-PegRNA-H1-Nicking sgRNA 5302 bp DNA circular SYN 07-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5302)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 21, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..5302
/mol_type="other DNA"
/organism="synthetic DNA construct"
promoter 72..320
/label=U6 promoter
/note="RNA polymerase III promoter for human U6 snRNA"
primer_bind 72..92
/label=hU6-F
/note="Human U6 promoter, forward primer"
primer_bind 243..262
/label=LKO.1 5'
/note="Human U6 promoter, forward primer"
primer_bind 318..343
/label=OL2770
misc_feature 321..340
/label=DNMT1 spacer
misc_RNA 341..416
/label=gRNA scaffold
/note="guide RNA scaffold for the Streptococcus pyogenes
CRISPR/Cas9 system"
misc_feature 417..427
/label=RT template
primer_bind complement(421..458)
/label=OL2771
misc_feature 423
/label=C352A
misc_feature 428..440
/label=PBS
misc_feature 448..663
/label=H1 promoter
primer_bind 451..467
/label=OL2772
primer_bind 583..602
/label=H1
/note="Human H1 promoter, forward primer"
primer_bind complement(655..697)
/label=OL2773
misc_feature 673..693
/label=DNMT1 nickingRNA
misc_RNA 694..769
/label=gRNA scaffold
/note="guide RNA scaffold for the Streptococcus pyogenes
CRISPR/Cas9 system"
misc_feature 821..938
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
promoter 987..1497
/label=hPGK promoter
/note="human phosphoglycerate kinase 1 promoter"
primer_bind 1416..1434
/label=hPGK-F
/note="Human PGK promoter, forward primer"
CDS 1519..2118
/codon_start=1
/gene="pac from Streptomyces alboniger"
/product="puromycin N-acetyltransferase"
/label=PuroR
/note="confers resistance to puromycin"
/translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
primer_bind complement(1519..1538)
/label=Puro-R
/note="Puromycin resistance gene, reverse primer. Also
called puro-variant-R"
primer_bind 2015..2035
/label=Puro-F
/note="Puromycin resistance gene, forward primer"
polyA_signal 2265..2386
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(2276..2295)
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind 2330..2349
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind complement(2634..2651)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(2805..3393)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(2885..2904)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(3564..4424)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 4187..4206
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(4425..4529)
/gene="bla"
/label=AmpR promoter
rep_origin 4556..5011
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(4643..4662)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 4853..4874
/label=F1ori-F
/note="F1 origin, forward primer"
polyA_signal 5142..5190
/note="synthetic polyadenylation signal"
misc_feature 5204..5295
/label=pause site
/note="RNA polymerase II transcriptional pause signal from
the human alpha-2 globin gene"
primer_bind 5244..5263
/label=RVprimer3
/note="pGL3 vector, forward primer"
ORIGIN
1 ggtaccgatt agtgaacgga tctcgacggt atcgatcacg agactagcct cgagcggccg
61 cccccttcac cgagggccta tttcccatga ttccttcata tttgcatata cgatacaagg
121 ctgttagaga gataattgga attaatttga ctgtaaacac aaagatatta gtacaaaata
181 cgtgacgtag aaagtaataa tttcttgggt agtttgcagt tttaaaatta tgttttaaaa
241 tggactatca tatgcttacc gtaacttgaa agtatttcga tttcttggct ttatatatct
301 tgtggaaagg acgaaacacc gattcctggt gccagaaaca gttttagagc tagaaatagc
361 aagttaaaat aaggctagtc cgttatcaac ttgaaaaagt ggcaccgagt cggtgcgtca
421 ccactgtttc tggcaccagg tttttttgaa cgctgacgtc atcaacccgc tccaaggaat
481 cgcgggccca gtgtcactag gcgggaacac ccagcgcgcg tgcgccctgg caggaagatg
541 gctgtgaggg acaggggagt ggcgccctgc aatatttgca tgtcgctatg tgttctggga
601 aatcaccata aacgtgaaat gtctttggat ttgggaatct tataagttct gtatgaggac
661 cacgaaacac cggcccttca gctaaaataa agggttttag agctagaaat agcaagttaa
721 aataaggcta gtccgttatc aacttgaaaa agtggcaccg agtcggtgct ttttttaaag
781 aattctcgac ctcgagacaa atggcagtat tcatccacaa ttttaaaaga aaagggggga
841 ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta
901 aagaattaca aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca
961 gagatccact ttggccgcgg ctcgaggggg ttggggttgc gccttttcca aggcagccct
1021 gggtttgcgc agggacgcgg ctgctctggg cgtggttccg ggaaacgcag cggcgccgac
1081 cctgggactc gcacattctt cacgtccgtt cgcagcgtca cccggatctt cgccgctacc
1141 cttgtgggcc ccccggcgac gcttcctgct ccgcccctaa gtcgggaagg ttccttgcgg
1201 ttcgcggcgt gccggacgtg acaaacggaa gccgcacgtc tcactagtac cctcgcagac
1261 ggacagcgcc agggagcaat ggcagcgcgc cgaccgcgat gggctgtggc caatagcggc
1321 tgctcagcag ggcgcgccga gagcagcggc cgggaagggg cggtgcggga ggcggggtgt
1381 ggggcggtag tgtgggccct gttcctgccc gcgcggtgtt ccgcattctg caagcctccg
1441 gagcgcacgt cggcagtcgg ctccctcgtt gaccgaatca ccgacctctc tccccagggg
1501 gatccaccgg agcttaccat gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac
1561 gacgtcccca gggccgtacg caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc
1621 cacaccgtcg atccggaccg ccacatcgag cgggtcaccg agctgcaaga actcttcctc
1681 acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg
1741 gtctggacca cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc
1801 atggccgagt tgagcggttc ccggctggcc gcgcagcaac agatggaagg cctcctggcg
1861 ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac
1921 cagggcaagg gtctgggcag cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc
1981 ggggtgcccg ccttcctgga aacctccgcg ccccgcaacc tccccttcta cgagcggctc
2041 ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc
2101 cgcaagcccg gtgcctgacg cccgccccac gacccgcagc gcccgaccga aaggagcgca
2161 cgaccccatg catcggtacc tttaagacca atgacttaca aggcagctgt agatcttagc
2221 cactttctag agtcggggcg gccggccgct tcgagcagac atgataagat acattgatga
2281 gtttggacaa accacaacta gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga
2341 tgctattgct ttatttgtaa ccattataag ctgcaataaa caagttaaca acaacaattg
2401 cattcatttt atgtttcagg ttcaggggga ggtgtgggag gttttttaaa gcaagtaaaa
2461 cctctacaaa tgtggtaaaa tcgataagga tccgtcgacc gatgcccttg agagccttca
2521 acccagtcag ctccttccgg tgggcgcggg gcatgactat cgtcgccgca cttatgactg
2581 tcttctttat catgcaactc gtaggacagg tgccggcagc gctcttccgc ttcctcgctc
2641 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
2701 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
2761 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
2821 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
2881 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
2941 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
3001 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
3061 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
3121 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
3181 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
3241 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
3301 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
3361 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
3421 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
3481 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
3541 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
3601 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
3661 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgggaccc acgctcaccg
3721 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
3781 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
3841 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
3901 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
3961 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
4021 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
4081 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
4141 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
4201 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
4261 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
4321 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
4381 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
4441 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
4501 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgcg
4561 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
4621 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
4681 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
4741 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg
4801 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
4861 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg
4921 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
4981 aattttaaca aaatattaac gcttacaatt tgccattcgc cattcaggct gcgcaactgt
5041 tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agcccaagct accatgataa
5101 gtaagtaata ttaaggtacg ggaggtactt ggagcggccg caataaaata tctttatttt
5161 cattacatct gtgtgttggt tttttgtgtg aatcgatagt actaacatac gctctccatc
5221 aaaacaaaac gaaacaaaac aaactagcaa aataggctgt ccccagtgca agtgcaggtg
5281 ccagaacatt tctctatcga ta
//
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pU6-DNMT1-PegRNA-H1-Nicking sgRNA质粒载体应用举例
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