psiCHECK-2-h1.82705/9

LOCUS       psiCHECK-2-h1.82705/9               6620 bp DNA     circular SYN 03-FEB-2026
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6620)
  AUTHORS   SnapGene License
  TITLE     Direct Submission
  JOURNAL   Exported Aug 26, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..6620
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        62..419
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        62..253
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     rep_origin      270..405
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          489..621
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     promoter        666..684
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      688..697
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             694..1629
                     /codon_start=1
                     /product="Renilla luciferase"
                     /label=hRluc
                     /note="human codon-optimized"
                     /translation="MASKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN
                     AVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW
                     FELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDI
                     ALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE
                     IPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKV
                     KGLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
     misc_feature    1643..1672
                     /label=Feature 18
     misc_feature    1681..2019
                     /label=-1
     misc_feature    1916..1927
                     /label=Feature 21
     misc_feature    1979..1991
                     /label=Feature 20
     misc_feature    2019..2028
                     /label=Feature 22
     misc_feature    2028..2057
                     /label=Feature 19
     polyA_signal    2035..2083
                     /note="synthetic polyadenylation signal"
     promoter        2092..2843
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     regulatory      2873..2882
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2879..4531
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /db_xref="GI:42718124"
                     /protein_id="AAS38485.1"
                     /translation="MADAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     RBS             3838..3846
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     polyA_signal    4575..4696
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        4829..4933
                     /gene="bla"
                     /label=AmpR promoter
     CDS             4934..5794
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      5965..6553
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 agatctgcgc agcaccatgg cctgaaataa cctctgaaag aggaacttgg ttaggtacct
       61 tctgaggcgg aaagaaccag ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag
      121 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg
      181 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
      241 caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc
      301 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg
      361 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa
      421 agcttgattc ttctgacaca acagtctcga acttaagctg cagaagttgg tcgtgaggca
      481 ctgggcaggt aagtatcaag gttacaagac aggtttaagg agaccaatag aaactgggct
      541 tgtcgagaca gagaagactc ttgcgtttct gataggcacc tattggtctt actgacatcc
      601 actttgcctt tctctccaca ggtgtccact cccagttcaa ttacagctct taaggctaga
      661 gtacttaata cgactcacta taggctagcc accatggctt ccaaggtgta cgaccccgag
      721 caacgcaaac gcatgatcac tgggcctcag tggtgggctc gctgcaagca aatgaacgtg
      781 ctggactcct tcatcaacta ctatgattcc gagaagcacg ccgagaacgc cgtgattttt
      841 ctgcatggta acgctgcctc cagctacctg tggaggcacg tcgtgcctca catcgagccc
      901 gtggctagat gcatcatccc tgatctgatc ggaatgggta agtccggcaa gagcgggaat
      961 ggctcatatc gcctcctgga tcactacaag tacctcaccg cttggttcga gctgctgaac
     1021 cttccaaaga aaatcatctt tgtgggccac gactgggggg cttgtctggc ctttcactac
     1081 tcctacgagc accaagacaa gatcaaggcc atcgtccatg ctgagagtgt cgtggacgtg
     1141 atcgagtcct gggacgagtg gcctgacatc gaggaggata tcgccctgat caagagcgaa
     1201 gagggcgaga aaatggtgct tgagaataac ttcttcgtcg agaccatgct cccaagcaag
     1261 atcatgcgga aactggagcc tgaggagttc gctgcctacc tggagccatt caaggagaag
     1321 ggcgaggtta gacggcctac cctctcctgg cctcgcgaga tccctctcgt taagggaggc
     1381 aagcccgacg tcgtccagat tgtccgcaac tacaacgcct accttcgggc cagcgacgat
     1441 ctgcctaaga tgttcatcga gtccgaccct gggttctttt ccaacgctat tgtcgaggga
     1501 gctaagaagt tccctaacac cgagttcgtg aaggtgaagg gcctccactt cagccaggag
     1561 gacgctccag atgaaatggg taagtacatc aagagcttcg tggagcgcgt gctgaagaac
     1621 gagcagtaat tctaggcgat cgctcgagcc cgggaattcg tttaaaccta gagcggccgc
     1681 cagaaatgtg aatgatgtcc ctcatgtatt gcttatttct tgagctttta ggtacaactt
     1741 ggcaaatata ccaaatgata gagaggaaaa atgaaggtat ttttacattg aaaatttttc
     1801 actggcaaag ctcttttcct gaaggaaaca tcactcatgt ttaggctaga ttatgtgcgc
     1861 atcgtgacct agaattggaa acccagtggc tactgaactg ggggctcctc taaagccaaa
     1921 ccaaaccatt tgagcaagaa ctatggacta ttcatacagt ttatcaaatt tagccagatg
     1981 acagagacag tgtctgacaa aaggttgaat ctagtaaaag cggccgctgg ccgcaataaa
     2041 atatctttat tttcattaca tctgtgtgtt ggttttttgt gtgaggatct aaatgagtct
     2101 tcggacctcg cgggggccgc ttaagcggtg gttagggttt gtctgacgcg gggggagggg
     2161 gaaggaacga aacactctca ttcggaggcg gctcggggtt tggtcttggt ggccacgggc
     2221 acgcagaaga gcgccgcgat cctcttaagc acccccccgc cctccgtgga ggcgggggtt
     2281 tggtcggcgg gtggtaactg gcgggccgct gactcgggcg ggtcgcgcgc cccagagtgt
     2341 gaccttttcg gtctgctcgc agacccccgg gcggcgccgc cgcggcggcg acgggctcgc
     2401 tgggtcctag gctccatggg gaccgtatac gtggacaggc tctggagcat ccgcacgact
     2461 gcggtgatat taccggagac cttctgcggg acgagccggg tcacgcggct gacgcggagc
     2521 gtccgttggg cgacaaacac caggacgggg cacaggtaca ctatcttgtc acccggaggc
     2581 gcgagggact gcaggagctt cagggagtgg cgcagctgct tcatccccgt ggcccgttgc
     2641 tcgcgtttgc tggcggtgtc cccggaagaa atatatttgc atgtctttag ttctatgatg
     2701 acacaaaccc cgcccagcgt cttgtcattg gcgaattcga acacgcagat gcagtcgggg
     2761 cggcgcggtc ccaggtccac ttcgcatatt aaggtgacgc gtgtggcctc gaacaccgag
     2821 cgaccctgca gcgacccgct taaaagcttg gcattccggt actgttggta aagccaccat
     2881 ggccgatgct aagaacatta agaagggccc tgctcccttc taccctctgg aggatggcac
     2941 cgctggcgag cagctgcaca aggccatgaa gaggtatgcc ctggtgcctg gcaccattgc
     3001 cttcaccgat gcccacattg aggtggacat cacctatgcc gagtacttcg agatgtctgt
     3061 gcgcctggcc gaggccatga agaggtacgg cctgaacacc aaccaccgca tcgtggtgtg
     3121 ctctgagaac tctctgcagt tcttcatgcc agtgctgggc gccctgttca tcggagtggc
     3181 cgtggcccct gctaacgaca tttacaacga gcgcgagctg ctgaacagca tgggcatttc
     3241 tcagcctacc gtggtgttcg tgtctaagaa gggcctgcag aagatcctga acgtgcagaa
     3301 gaagctgcct atcatccaga agatcatcat catggactct aagaccgact accagggctt
     3361 ccagagcatg tacacattcg tgacatctca tctgcctcct ggcttcaacg agtacgactt
     3421 cgtgccagag tctttcgaca gggacaaaac cattgccctg atcatgaaca gctctgggtc
     3481 taccggcctg cctaagggcg tggccctgcc tcatcgcacc gcctgtgtgc gcttctctca
     3541 cgcccgcgac cctattttcg gcaaccagat catccccgac accgctattc tgagcgtggt
     3601 gccattccac cacggcttcg gcatgttcac caccctgggc tacctgattt gcggctttcg
     3661 ggtggtgctg atgtaccgct tcgaggagga gctgttcctg cgcagcctgc aagactacaa
     3721 aattcagtct gccctgctgg tgccaaccct gttcagcttc ttcgctaaga gcaccctgat
     3781 cgacaagtac gacctgtcta acctgcacga gattgcctct ggcggcgccc cactgtctaa
     3841 ggaggtgggc gaagccgtgg ccaagcgctt tcatctgcca ggcatccgcc agggctacgg
     3901 cctgaccgag acaaccagcg ccattctgat taccccagag ggcgacgaca agcctggcgc
     3961 cgtgggcaag gtggtgccat tcttcgaggc caaggtggtg gacctggaca ccggcaagac
     4021 cctgggagtg aaccagcgcg gcgagctgtg tgtgcgcggc cctatgatta tgtccggcta
     4081 cgtgaataac cctgaggcca caaacgccct gatcgacaag gacggctggc tgcactctgg
     4141 cgacattgcc tactgggacg aggacgagca cttcttcatc gtggaccgcc tgaagtctct
     4201 gatcaagtac aagggctacc aggtggcccc agccgagctg gagtctatcc tgctgcagca
     4261 ccctaacatt ttcgacgccg gagtggccgg cctgcccgac gacgatgccg gcgagctgcc
     4321 tgccgccgtc gtcgtgctgg aacacggcaa gaccatgacc gagaaggaga tcgtggacta
     4381 tgtggccagc caggtgacaa ccgccaagaa gctgcgcggc ggagtggtgt tcgtggacga
     4441 ggtgcccaag ggcctgaccg gcaagctgga cgcccgcaag atccgcgaga tcctgatcaa
     4501 ggctaagaaa ggcggcaaga tcgccgtgta ataattctag agtcggggcg gccggccgct
     4561 tcgagcagac atgataagat acattgatga gtttggacaa accacaacta gaatgcagtg
     4621 aaaaaaatgc tttatttgtg aaatttgtga tgctattgct ttatttgtaa ccattataag
     4681 ctgcaataaa caagttaaca acaacaattg cattcatttt atgtttcagg ttcaggggga
     4741 ggtgtgggag gttttttaaa gcaagtaaaa cctctacaaa tgtggtaaaa tcgataagga
     4801 tccaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
     4861 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
     4921 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
     4981 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga
     5041 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga
     5101 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg
     5161 cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc
     5221 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac
     5281 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact
     5341 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca
     5401 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg
     5461 tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact
     5521 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg
     5581 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
     5641 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
     5701 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
     5761 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat
     5821 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
     5881 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
     5941 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     6001 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     6061 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
     6121 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     6181 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     6241 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     6301 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     6361 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     6421 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     6481 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     6541 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
     6601 ttttgctcac atggctcgac
//