pYK405

LOCUS      pYK405                8495 bp DNA     circular SYN 11-FEB-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8495)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 27, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..8495
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     213..232
                     /label=oYK403_MluI_F
     misc_feature    232..819
                     /label=CMV
     primer_bind     863..882
                     /label=T7
     primer_bind     880..984
                     /label=oCW60
     primer_bind     881..989
                     /label=oYK209_FUS_F
     primer_bind     881..900
                     /label=oYK06-ANR-mEGFP-F
     primer_bind     881..900
                     /label=oYK30-NS3a_Head-F
     promoter        881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    905..964
                     /label=NLS
     primer_bind     964..989
                     /label=oYK374_FUS_F
     primer_bind     964..984
                     /label=oCW41
     CDS             965..1609
                     /codon_start=1
                     /gene="FUS"
                     /gene_synonym="ALS6; altFUS; ETM4; FUS1; HNRNPP2; 
                     POMP75;TLS"
                     /product="RNA-binding protein FUS isoform 1"
                     /label=FUS
                     /note="isoform 1 is encoded by transcript variant 1; 
                     heterogeneous nuclear ribonucleoprotein P2; fused in 
                     sarcoma; fusion gene in myxoid liposarcoma; 75 kDa 
                     DNA-pairing protein; RNA-binding protein FUS; oncogene TLS;
                     fus-like protein; oncogene FUS; translocated in liposarcoma
                     protein"
                     /db_xref="CCDS:CCDS10707.1"
                     /db_xref="GeneID:2521"
                     /db_xref="HGNC:HGNC:4010"
                     /db_xref="MIM:137070"
                     /protein_id="NP_004951.1"
                     /translation="MASNDYTQQATQSYGAYPTQPGQGYSQQSSQPYGQQSYSGYSQST
                     DTSGYGQSSYSSYGQSQNTGYGTQSTPQGYGSTGGYGSSQSSQSSYGQQSSYPGYGQQP
                     APSSTSGSYGSSSQSSSYGQPQSGSYSQQPSYGGQQQSYGQQQSYNPPQGYGQQNQYNS
                     SSGGGGGGGGGGNYGQDQSSMSSGGGSGGGYGNQDQSGGGGSGGYGQQDRGG"
     primer_bind     968..993
                     /label=oYK339_FUS_F
     misc_feature    1040..1042
                     /gene="FUS"
                     /gene_synonym="ALS6; altFUS; ETM4; FUS1; HNRNPP2; 
                     POMP75;TLS"
                     /note="Phosphoserine./evidence=ECO:0000269|PubMed:29897835;
                      propagated from UniProtKB/Swiss-Prot (P35637.1); 
                     phosphorylation site"
     misc_feature    1052..1054
                     /gene="FUS"
                     /gene_synonym="ALS6; altFUS; ETM4; FUS1; HNRNPP2; 
                     POMP75;TLS"
                     /note="Phosphoserine./evidence=ECO:0000269|PubMed:29897835;
                      propagated from UniProtKB/Swiss-Prot (P35637.1); 
                     phosphorylation site"
     misc_feature    1088..1090
                     /gene="FUS"
                     /gene_synonym="ALS6; altFUS; ETM4; FUS1; HNRNPP2; 
                     POMP75;TLS"
                     /note="Phosphoserine, by ATM. 
                     /evidence=ECO:0000269|PubMed:18620545; propagated from 
                     UniProtKB/Swiss-Prot (P35637.1); phosphorylation site"
     misc_feature    1487..1489
                     /gene="FUS"
                     /gene_synonym="ALS6; altFUS; ETM4; FUS1; HNRNPP2; 
                     POMP75;TLS"
                     /note="Breakpoint for translocation to form 
                     chimericFUS/ATF1 protein; propagated from 
                     UniProtKB/Swiss-Prot (P35637.1); other site"
     primer_bind     complement(1579..1611)
                     /label=oCW42
     primer_bind     complement(1579..1611)
                     /label=oCW61
     primer_bind     1590..1631
                     /label=oYK68_FUS2_F
     primer_bind     1590..1609
                     /label=oYK69_AG_ANR1_F
     primer_bind     complement(1594..1609)
                     /label=oYK68_FUS1-R
     misc_feature    1610..2398
                     /label=FUS215-478
     primer_bind     1698..1712
                     /label=oYK68_FUS2_F
     primer_bind     complement(2377..2418)
                     /label=oYK88_FUS478_R
     misc_feature    2399..2626
                     /label=Long_Linker
     primer_bind     2399..2418
                     /label=oYK78_AG_ANR1_F
     primer_bind     2399..2416
                     /label=oYK69_AG_ANR1_F
     misc_feature    2627..3301
                     /label=AG
     primer_bind     3075..3085
                     /label=oSCL1104
     primer_bind     complement(3289..3309)
                     /label=oYK65-AG-R
     misc_feature    3302..3352
                     /label=Short_Linker
     primer_bind     complement(3328..3352)
                     /label=oYK33-35-PB1-AG-R
     primer_bind     3333..3373
                     /label=oYK153_NS3a_F
     CDS             3353..3940
                     /codon_start=1
                     /label=NS3a H1
                     /translation="KKKGSVVIVGRINLSGDTAYSQQTRGLEGCQETSQTGRDKNQVEG
                     EVQVVSTATQSFLATSINGVLWTVYHGAGTRTIASPKGPVTQMYTNVDKDLVGWQAPQG
                     SRSLTPCTCGSSDLYLVTRHADVIPVRGRGDSRGSLLSPRPISYLKGSSGGPLLCPAGH
                     AVGIFRAAVSTRGVAKAVDFIPVESLETTMRSP"
     primer_bind     3353..3388
                     /label=oSCL1082
     primer_bind     3353..3373
                     /label=oyk03-03-F
     primer_bind     3353..3373
                     /label=oYK04-ns3a-F
     primer_bind     3353..3371
                     /label=oYK25-NS3a-F
     primer_bind     3353..3370
                     /label=oYK30-NS3a_Head-F
     primer_bind     3370..3428
                     /label=oSCL1083
     primer_bind     3409..3467
                     /label=oSCL1084
     primer_bind     3446..3504
                     /label=oSCL1085
     primer_bind     3483..3541
                     /label=oSCL1086
     primer_bind     complement(3511..3530)
                     /label=oSCL1106
     primer_bind     3512..3550
                     /label=oSCL1104
     primer_bind     complement(3747..3805)
                     /label=oSCL1105
     primer_bind     complement(3787..3858)
                     /label=oSCL1087
     primer_bind     3789..3810
                     /label=oSCL1107
     primer_bind     3840..3858
                     /label=oSCL1088
     primer_bind     complement(3922..3946)
                     /label=oSCL1089
     primer_bind     complement(3925..3947)
                     /label=oYK30-NS3a_head-R
     primer_bind     complement(3928..3961)
                     /label=oYK405_NS3a_R
                     /note="for 3XFLAG"
     primer_bind     complement(3928..3947)
                     /label=oYK319_NS3a_R
     primer_bind     complement(3928..3946)
                     /label=oYK374_NS3a_R
     CDS             3947..4012
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     primer_bind     complement(4017..4036)
                     /label=oSCL1089
     terminator      4089..4316
                     /label=bGH PA
     misc_feature    4984..5061
                     /label=SV40 ORI
     CDS             5203..5994
                     /label=Kan/neoR
     primer_bind     complement(6334..6354)
                     /label=M13-rev
     misc_binding    complement(6360..6382)
                     /label=LacO
     promoter        complement(6387..6416)
                     /label=lac
     primer_bind     6531..6542
                     /label=oYK69_AG_ANR1_F
     CDS             complement(7502..8161)
                     /label=AmpR
     primer_bind     7818..7829
                     /label=oyk03-03-F
     primer_bind     7818..7829
                     /label=oYK04-ns3a-F
     primer_bind     7818..7829
                     /label=oYK153_NS3a_F
     promoter        complement(8401..8429)
                     /label=Amp prom
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 caccatgccc aagaagaagc gcaaggtgga agctagcgct tccccgaaga aaaagcggaa
      961 agtcatggcc tcaaacgatt atacccaaca agcaacccaa agctatgggg cctaccccac
     1021 ccagcccggg cagggctatt cccagcagag cagtcagccc tacggacagc agagttacag
     1081 tggttatagc cagtccacgg acacttcagg ctatggccag agcagctatt cttcttatgg
     1141 ccagagccag aacacaggct atggaactca gtcaactccc cagggatatg gctcgactgg
     1201 cggctatggc agtagccaga gctcccaatc gtcttacggg cagcagtcct cctaccctgg
     1261 ctatggccag cagccagctc ccagcagcac ctcgggaagt tacggtagca gttctcagag
     1321 cagcagctat gggcagcccc agagtgggag ctacagccag cagcctagct atggtggaca
     1381 gcagcaaagc tatggacagc agcaaagcta taatccccct cagggctatg gacagcagaa
     1441 ccagtacaac agcagcagtg gtggtggagg tggaggtgga ggtggaggta actatggcca
     1501 agatcaatcc tccatgagta gtggtggtgg cagtggtggc ggttatggca atcaagacca
     1561 gagtggtgga ggtggcagcg gtggctatgg acagcaggac cgtggaggca gaggcagagg
     1621 aggaagcgga ggaggaggag ggggaggagg aggcggatac aacagaagca gcggaggata
     1681 cgagccaaga ggaagaggag gaggaagagg agggagagga ggaatgggag gaagcgacag
     1741 aggaggattc aacaagttcg gaggaccaag agaccaggga agcagacacg acagcgagca
     1801 ggacaacagc gacaacaaca caatcttcgt gcagggactg ggcgagaatg tgaccatcga
     1861 gagcgtggcc gactacttca agcagatcgg aatcatcaag acaaacaaga aaacaggaca
     1921 gcccatgatc aacctgtaca ccgacagaga gactggcaag ctgaagggag aggccaccgt
     1981 gagcttcgac gaccccccca gcgccaaagc cgccatcgac tggttcgacg gcaaggagtt
     2041 cagcggcaac ccaatcaaag tgagctttgc caccagaaga gccgacttca acagaggcgg
     2101 aggcaacgga agaggcggaa gaggaagagg ggggccaatg ggcagaggag ggtatggcgg
     2161 aggaggaagc gggggaggag ggagaggggg attcccaagc ggaggagggg ggggaggagg
     2221 ccagcagaga gctggagact ggaagtgccc aaacccaaca tgcgagaaca tgaacttcag
     2281 ctggagaaac gagtgcaacc agtgcaaggc cccaaagcca gatggacccg gcggcggacc
     2341 aggaggaagc catatgggcg gcaactacgg cgacgacaga aggggcggca gaggcggagg
     2401 ttctgggagt ggcggtagtg gtgctggagg cagcgcaggc tctggagcag gcggcagtgc
     2461 cggaagtggc gctggagggt ctgccggatc tggagccggt ggttctgccg gctccggcgc
     2521 tggtgggagc gcttccggaa gtagtcccgg taattccgct gacggcggcg gaggatcggg
     2581 tggtagtggt ggttcaggag gaggatcgac ccaaggagga tccatggtga gcgtgatcaa
     2641 gcccgagatg aagatcaagc tgtgcatgag gggcaccgtg aacggccaca acttcgtgat
     2701 cgagggcgag ggcaagggca acccctacga gggcacccag atcctggacc tgaacgtgac
     2761 cgagggcgcc cccctgccct tcgcctacga catcctgacc accgtgttcc agtacggcaa
     2821 cagggccttc accaagtacc ccgccgacat ccaggactac ttcaagcaga ccttccccga
     2881 gggctaccac tgggagagga gcatgaccta cgaggaccag ggcatctgca ccgccaccag
     2941 caacatcagc atgaggggcg actgcttctt ctacgacatc aggttcgacg gcgtgaactt
     3001 cccccccaac ggccccgtga tgcagaagaa gaccctgaag tgggagccca gcaccgagaa
     3061 gatgtacgtg agggacggcg tgctgaaggg cgacgtgaac atggccctgc tgctggaggg
     3121 cggcggccac tacaggtgcg acttcaagac cacctacaag gccaagaagg acgtgaggct
     3181 gcccgactac cacttcgtgg accacaggat cgagatcctg aagcacgaca aggactacaa
     3241 caaggtgaag ctgtacgaga acgccgtggc caggtacagc atgctgccca gccaggccaa
     3301 gggtaccgga actgcagcag agaattcggg aaactcgaga acaaagcttg aaaagaagaa
     3361 gggcagcgtg gtgatcgtgg gcagaatcaa cctgagcggc gacaccgcct acagccagca
     3421 gaccagaggc ctggagggct gccaggagac cagccagacc ggcagagaca agaaccaggt
     3481 ggagggcgag gtgcaggtgg tgagcaccgc cacccagagc ttcctggcca ccagcatcaa
     3541 cggcgtgctg tggaccgtgt accacggcgc cggcaccaga accatcgcca gccccaaggg
     3601 ccccgtgacc cagatgtaca cgaacgtgga caaggactta gtgggctggc aggcccccca
     3661 aggcagcaga agcctgaccc cctgcacctg cggcagcagc gacctgtacc tggtgaccag
     3721 acacgccgac gtgatccccg tgagaggaag aggcgacagc agaggcagcc tgctgagccc
     3781 cagacccatc agctatctga aaggctcaag cggcggcccc ctgttgtgtc ccgccggcca
     3841 tgccgtgggc atcttcagag ccgccgtgag caccagaggc gtggccaagg ccgtggactt
     3901 catccccgtg gagagcctgg agaccaccat gagaagcccc gctagcgact acaaagacca
     3961 tgacggtgat tataaagatc atgacatcga ttacaaggat gacgatgaca agtaagaatt
     4021 ctgcagatat ccagcacagt ggcggccgct cgagtctaga gggcccgttt aaacccgctg
     4081 atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc
     4141 ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc
     4201 atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa
     4261 gggggaggat tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc
     4321 tgaggcggaa agaaccagct ggggctctag ggggtatccc cacgcgccct gtagcggcgc
     4381 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
     4441 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
     4501 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
     4561 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt
     4621 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg
     4681 aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc
     4741 ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt aattctgtgg
     4801 aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
     4861 agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc
     4921 agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg
     4981 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt
     5041 ttttttattt atgcagaggc cgaggccgcc tctgcctctg agctattcca gaagtagtga
     5101 ggaggctttt ttggaggcct aggcttttgc aaaaagctcc cgggagcttg tatatccatt
     5161 ttcggatctg atcaagagac aggatgagga tcgtttcgca tgattgaaca agatggattg
     5221 cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg ggcacaacag
     5281 acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg cccggttctt
     5341 tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc aggacgaggc agcgcggcta
     5401 tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg
     5461 ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atctcacctt
     5521 gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca tacgcttgat
     5581 ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg
     5641 atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg gctcgcgcca
     5701 gccgaactgt tcgccaggct caaggcgcgc atgcccgacg gcgaggatct cgtcgtgacc
     5761 catggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc tggattcatc
     5821 gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc tacccgtgat
     5881 attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc
     5941 gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt ctgagcggga
     6001 ctctggggtt cgaaatgacc gaccaagcga cgcccaacct gccatcacga gatttcgatt
     6061 ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga
     6121 tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccccaac ttgtttattg
     6181 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
     6241 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgta
     6301 taccgtcgac ctctagctag agcttggcgt aatcatggtc atagctgttt cctgtgtgaa
     6361 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct
     6421 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc
     6481 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
     6541 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
     6601 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
     6661 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     6721 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     6781 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     6841 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     6901 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     6961 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     7021 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     7081 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     7141 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
     7201 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     7261 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     7321 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     7381 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     7441 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
     7501 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     7561 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
     7621 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
     7681 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
     7741 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
     7801 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
     7861 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
     7921 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
     7981 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
     8041 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
     8101 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
     8161 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
     8221 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
     8281 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
     8341 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
     8401 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
     8461 cgcgcacatt tccccgaaaa gtgccacctg acgtc
//