pAAVLINKv1-pCALM1-Cre-3'-ECPAS-FLAG

LOCUS       pAAVLINKv1-pCALM1-Cre-3'-ECPAS-FLAG              7282 bp DNA     circular SYN 10-AUG-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7282)
  AUTHORS   Stavrini Papadaki
  TITLE     Direct Submission
  JOURNAL   Exported Jun 11, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..7282
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
                     /note="color: #ffe4c4"
     promoter        145..265
                     /label=pCALM1
     CDS             277..1308
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
                     LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
                     PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
                     LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
                     RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
                     RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
                     DGD"
     intron          1309..1349
                     /label=Splicing Donor
     protein_bind    1356..1389
                     /label=lox2272-JTZ17
                     /bound_moiety="Cre recombinase"
                     /note="Right element (RE) mutant of loxP (Araki et al., 
                     2010). Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     intron          1390..1481
                     /label=Splicing Acceptor
                     /note="Splicing acceptor"
     CDS             1482..3995
                     /codon_start=1
                     /gene="ECPAS"
                     /gene_synonym="ECM29; KIAA0368"
                     /product="proteasome adapter and scaffold protein 
                     ECM29isoform 3"
                     /label=ECPAS
                     /note="isoform 3 is encoded by transcript variant 3;homolog
                     of yeast Ecm29; ECM29 homolog, proteasome accessory 
                     protein; proteasome-associated protein ECM29 homolog; ecm29
                     proteasome adapter and scaffold; proteasome adapter and 
                     scaffold protein ECM29"
                     /db_xref="GI:1428713215"
                     /db_xref="CCDS:CCDS94456.1"
                     /db_xref="GeneID:23392"
                     /db_xref="HGNC:HGNC:29020"
                     /db_xref="MIM:616694"
                     /protein_id="NP_001351858.1"
                     /translation="GLGLVYELGNEQDQQELVSTLVETLMTGKRVKHEVSGETVVFQGG
                     ALGKTPDGQGLSTYKELCSLASDLSQPDLVYKFMNLANHHAMWNSRKGAAFGFNVIATR
                     AGEQLAPFLPQLVPRLYRYQFDPNLGIRQAMTSIWNALVTDKSMVDKYLKEILQDLVKN
                     LTSNMWRVRESSCLALNDLLRGRPLDDIIDKLPEIWETLFRVQDDIKESVRKAAELALK
                     TLSKVCVKMCDPAKGAAGQRTIAALLPCLLDKGMMSTVTEVRALSINTLVKISKSAGAM
                     LKPHAPKLIPALLESLSVLEPQVLNYLSLRATEQEKAAMDSARLSAAKSSPMMETINMC
                     LQYLDVSVLGELVPRLCELIRSGVGLGTKGGCASVIVSLTTQCPQDLTPYSGKLMSALL
                     SGLTDRNSVIQKSCAFAMGHLVRTSRDSSTEKLLQKLNGWYMEKEEPIYKTSCALTIHA
                     IGRYSPDVLKNHAKEVLPLAFLGMHEIADEEKSEKEECNLWTEVWQENVPGSFGGIRLY
                     LQELITITQKALQSQSWKMKAQGAIAMASIAKQTSSLVPPYLGMILTALLQGLAGRTWA
                     GKEELLKAIACVVTACSAELEKSVPNQPSTNEILQAVLKECSKENVKYKIVAISCAADI
                     LKATKEDRFQEFSNIVIPLIKKNSLESSGVRTTKNEEENEKEKELQLEYLLGAFESLGK
                     AWPRNAETQRCYRQELCKLMCERLKLSTWKVQLGVLQSMNAFFQGLMLLEEEHADPEAL
                     AEILLETCKSITYSLENKTYSSVRTEALSVIELLLKKLEESKQWECLTSECRVLLIESL
                     ATMEPDSRPELQEKAALLKKTLENLE"
     misc_feature    3214..3219
                     /label=SDS
     CDS             4002..4067
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     terminator      4071..4502
                     /label=W3SL
                     /note="Short WPRE and SV40 polyA"
     misc_feature    4215..4220
                     /label=SDS
     repeat_region   4545..4685
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     misc_feature    4545..4674
                     /label=AAV2 ITR
     repeat_region   4546..4686
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     rep_origin      4760..5215
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        5497..5601
                     /gene="bla"
                     /label=AmpR promoter
     CDS             5602..6462
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      6633..7221
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtcgctct gcgcgaggcg gctccgccgc ggcggaggga
      181 tacggcgcac catatatata tcgcggggcg cagactcgcg ctccggcagt ggtgctggga
      241 gtgtcgtgga cgccgtgccg ttactcgtac gccaccatgt ccaatttact gaccgtacac
      301 caaaatttgc ctgcattacc ggtcgatgca acgagtgatg aggttcgcaa gaacctgatg
      361 gacatgttca gggatcgcca ggcgttttct gagcatacct ggaaaatgct tctgtccgtt
      421 tgccggtcgt gggcggcatg gtgcaagttg aataaccgga aatggtttcc cgcagaacct
      481 gaagatgttc gcgattatct tctatatctt caggcgcgcg gtctggcagt aaaaaccatc
      541 cagcaacatt tgggccagct aaacatgctt catcgtcggt ccgggctgcc acgaccaagt
      601 gacagcaatg ctgtttcact ggttatgcgg cggatccgaa aagaaaacgt tgatgccggt
      661 gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt tcgaccaggt tcgttcactc
      721 atggaaaata gcgatcgctg ccaggatata cgtaatctgg catttctggg gattgcttat
      781 aacaccctgt tacgtatagc cgaaattgcc aggatcaggg ttaaagatat ctcacgtact
      841 gacggtggga gaatgttaat ccatattggc agaacgaaaa cgctggttag caccgcaggt
      901 gtagagaagg cacttagcct gggggtaact aaactggtcg agcgatggat ttccgtctct
      961 ggtgtagctg atgatccgaa taactacctg ttttgccggg tcagaaaaaa tggtgttgcc
     1021 gcgccatctg ccaccagcca gctatcaact cgcgccctgg aagggatttt tgaagcaact
     1081 catcgattga tttacggcgc taaggatgac tctggtcaga gatacctggc ctggtctgga
     1141 cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg ctggagtttc aataccggag
     1201 atcatgcaag ctggtggctg gaccaatgta aatattgtaa tgaactatat ccgtaacctg
     1261 gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg gcgattaggt aagtatcaag
     1321 gttacaagac aggtttaagg agaccaataa ctagtataac ttcgtatagg atactttata
     1381 gcaatttatg aaactgggct tgtcgagaca gagaagactc ttgcgtttct gataggcacc
     1441 tattggtctt actgacatcc actttgcctt tctctccaca gggccttggg ttggtttatg
     1501 aactaggcaa tgaacaagat caacaggaat tggtttctac acttgtggaa acacttatga
     1561 ctggcaaaag agttaaacat gaagtttctg gagagacagt ggtatttcaa gggggagctc
     1621 ttggcaaaac accagatggt cagggccttt ctacttacaa ggaactttgt tctctggcaa
     1681 gtgatcttag ccagccagat ctggtgtata aatttatgaa tttagccaac catcatgcaa
     1741 tgtggaactc taggaagggt gctgcttttg gttttaatgt aattgctacc agagctggag
     1801 agcagctggc tccttttctg cctcagctag ttcctcgact ttatcgttac cagtttgatc
     1861 ccaaccttgg cattcgacag gccatgacaa gtatttggaa tgcgttggtc actgacaaat
     1921 ccatggtgga taaatatttg aaagaaattc ttcaagattt ggttaagaac cttacaagca
     1981 atatgtggcg agttcgagaa tccagctgtt tagctttgaa tgatttattg agaggaagac
     2041 ccttagatga catcattgat aaacttccag aaatttggga aacgcttttt agagtacaag
     2101 atgatatcaa ggaatctgta cgaaaagcgg cagaactagc tctgaaaact ctgagcaagg
     2161 tctgtgtgaa aatgtgtgac cctgccaaag gagcagctgg ccagagaacc atcgctgccc
     2221 ttctgccttg ccttctggac aaaggaatga tgagcaccgt gacggaagtt cgagccctca
     2281 gcattaacac ccttgtgaag atcagcaaaa gtgcaggagc catgttgaaa ccgcatgcac
     2341 caaaactcat tccagctctg ctagagtcct taagtgtatt ggagccccaa gttctcaatt
     2401 atttgagcct ccgggcgaca gagcaagaaa aggctgcgat ggatagtgct cggcttagtg
     2461 ctgccaaatc ttctccaatg atggaaacaa tcaacatgtg cctgcaatac cttgatgtgt
     2521 cagtgctggg cgagctagtt cctaggttgt gtgaactgat cagaagtggt gtaggtcttg
     2581 gaactaaggg tggctgtgcc agtgtcattg tgtcattaac tactcagtgt cctcaggacc
     2641 taacacctta ctcaggtaaa cttatgagtg ctttgctgag tggcctgaca gatcggaaca
     2701 gtgtgattca gaaatcttgt gcatttgcta tgggccattt agttcggacc tcacgggata
     2761 gcagcactga aaaactcctg cagaagctca atgggtggta tatggagaaa gaagaaccta
     2821 tctacaagac ctcttgtgct ttgactattc atgctattgg acgatacagc cctgatgtat
     2881 taaagaatca tgcaaaagaa gtcctgcctc tggcattttt aggcatgcat gaaattgctg
     2941 atgaggagaa atccgaaaaa gaagaatgta atttatggac cgaagtgtgg caggaaaacg
     3001 tacctggatc ctttggtggc attcgattat acctgcagga gttaattact attacccaga
     3061 aggctttgca gtctcagtcc tggaaaatga aagcccaggg tgcaattgcc atggcatcaa
     3121 ttgcaaaaca gactagctct ctagtacctc catatctcgg aatgatactg accgcattgc
     3181 tgcaaggcct ggctggaaga acgtgggcag gaaaggagga gctattgaaa gccattgcct
     3241 gtgtggtgac agcttgcagt gcagagctgg aaaagtctgt gcccaatcaa cccagcacaa
     3301 atgaaattct tcaagctgtt ctgaaggaat gtagcaaaga gaatgtcaaa tacaagattg
     3361 tagcaatcag ctgtgcagct gatatcttga aggccaccaa agaggacaga ttccaggagt
     3421 tctctaacat tgtcatacct ctcatcaaga agaactcact tgaaagcagt ggggtccgga
     3481 caaccaaaaa tgaagaggag aatgaaaagg aaaaggagct ccagctggaa tatctgctgg
     3541 gtgcctttga aagcctgggc aaagcctggc cgcgaaacgc ggagacccaa cgttgttatc
     3601 gtcaggagct gtgcaaactg atgtgtgaac ggctaaaact cagcacgtgg aaagtgcagc
     3661 taggagtcct gcaatcaatg aatgcctttt ttcaggggtt aatgcttttg gaagaagaac
     3721 atgccgatcc tgaggctttg gctgaaattc tgcttgaaac ttgtaaatca atcacatatt
     3781 ctttagaaaa taagacctac tcatctgtga gaacagaagc tttatctgtg atagaattgc
     3841 tgcttaaaaa acttgaagaa tctaaacagt gggaatgttt gacatctgaa tgcagagtgc
     3901 tcctaattga gtctttagct actatggagc cagacagcag acctgaactg caggagaaag
     3961 cagcgttact gaagaaaaca cttgaaaatc tggaatgtac agactacaaa gaccatgacg
     4021 gtgattataa agatcatgac atcgactaca aggatgacga tgacaagtaa ctcgagataa
     4081 tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc
     4141 ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat
     4201 ggctttcatt ttctcctcct tgtataaatc ctggttagtt cttgccacgg cggaactcat
     4261 cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt
     4321 ggtgtttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatct agctttattt
     4381 gtgaaatttg tgatgctatt gctttatttg taaccattat aagctgcaat aaacaagtta
     4441 acaacaacaa ttgcattcat tttatgtttc aggttcaggg ggagatgtgg gaggtttttt
     4501 aaagatctgg agaccggtgt ggcacgtgcg gaccgagcgg ccgcaggaac ccctagtgat
     4561 ggagttggcc actccctctc tgcgcgctcg ctcgctcact gaggccgggc gaccaaaggt
     4621 cgcccgacgc ccgggctttg cccgggcggc ctcagtgagc gagcgagcgc gcagctgcct
     4681 gcaggggcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat
     4741 acgtcaaagc aaccatagta cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
     4801 gttacgcgca gcgtgaccgc tacacttgcc agcgccttag cgcccgctcc tttcgctttc
     4861 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc
     4921 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgatttgggt
     4981 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag
     5041 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ctctatctcg
     5101 ggctattctt ttgatttata agggattttg ccgatttcgg tctattggtt aaaaaatgag
     5161 ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgtttac aattttatgg
     5221 tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagccccg acacccgcca
     5281 acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct
     5341 gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg
     5401 agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt
     5461 tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
     5521 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
     5581 taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt
     5641 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat
     5701 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag
     5761 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg
     5821 ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata
     5881 cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat
     5941 ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc
     6001 aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg
     6061 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac
     6121 gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact
     6181 ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa
     6241 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct
     6301 ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc
     6361 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga
     6421 cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac
     6481 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag
     6541 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
     6601 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     6661 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     6721 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt
     6781 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     6841 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     6901 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     6961 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     7021 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     7081 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     7141 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     7201 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
     7261 tgctggcctt ttgctcacat gt
//