| 质粒名称: |
pAAVLINK-EF1alpha-HA-5'-KDM6A
|
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001838 |
| 产品用途: | 5' AAVLINK plasmid for KDM6A expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒 |
| 启动子: | EF1alpha |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | KDM6A |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
pAAVLINK-EF1alpha-HA-5'-KDM6A
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAVLINK-EF1alpha-HA-5'-KDM6A载体质粒基本信息
pAAVLINK-EF1alpha-HA-5'-KDM6A质粒图谱载体图谱和pAAVLINK-EF1alpha-HA-5'-KDM6A载体序列质粒序列多克隆位点信息
pAAVLINK-EF1alpha-HA-5'-KDM6A质粒载体简介
pAAVLINK-EF1alpha-HA-5'-KDM6A质粒序列载体序列
LOCUS pAAVLINK-EF1alpha-HA-5'-KDM6A 5837 bp DNA circular SYN 10-AUG-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5837)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported Jun 10, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..5837
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
/note="color: #ffe4c4"
promoter 158..700
/label=EF1-alpha
/note="Chimeric promoter of ef1a and htlv 5' UTR"
promoter 187..398
/label=EF-1-alpha core promoter
/note="core promoter for human elongation factor
EF-1-alpha"
LTR 411..679
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from human
T-cell leukemia virus (HTLV) type 1"
misc_feature 505..510
/label=SDS
CDS 716..742
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/label=HA
/translation="YPYDVPDYA"
CDS 749..2977
/codon_start=1
/gene="KDM6A"
/gene_synonym="bA386N14.2; KABUK2; UTX"
/product="lysine-specific demethylase 6A isoform 1"
/EC_number="1.14.11.68"
/label=KDM6A
/note="isoform 1 is encoded by transcript variant 1;
ubiquitously-transcribed TPR gene on the X chromosome;
ubiquitously transcribed tetratricopeptide repeat protein
X-linked; lysine (K)-specific demethylase 6A; bA386N14.2
(ubiquitously transcribed X chromosome tetratricopeptide
repeat protein (UTX)); histone demethylase UTX; [histone
H3]-trimethyl-L-lysine(27) demethylase 6A"
/db_xref="GI:611434998"
/db_xref="CCDS:CCDS94599.1"
/db_xref="GeneID:7403"
/db_xref="HGNC:HGNC:12637"
/db_xref="MIM:300128"
/protein_id="NP_001278344.1"
/translation="KSCGVSLATAAAAAAAFGDEEKKMAAGKASGESEEASPSLTAEER
EALGGLDSRLFGFVRFHEDGARTKALLGKAVRCYESLILKAEGKVESDFFCQLGHFNLL
LEDYPKALSAYQRYYSLQSDYWKNAAFLYGLGLVYFHYNAFQWAIKAFQEVLYVDPSFC
RAKEIHLRLGLMFKVNTDYESSLKHFQLALVDCNPCTLSNAEIQFHIAHLYETQRKYHS
AKEAYEQLLQTENLSAQVKATVLQQLGWMHHTVDLLGDKATKESYAIQYLQKSLEADPN
SGQSWYFLGRCYSSIGKVQDAFISYRQSIDKSEASADTWCSIGVLYQQQNQPMDALQAY
ICAVQLDHGHAAAWMDLGTLYESCNQPQDAIKCYLNATRSKSCSNTSALAARIKYLQAQ
LCNLPQGSLQNKTKLLPSIEEAWSLPIPAELTSRQGAMNTAQQACKPHHPNTEPVLGLS
QTPISQQSLPLHMIPSSQVDDLSSPAKRKRTSSPTKNTSDNWSGGHAVSHPPVQQQAHS
WCLTPQKLQHLEQLRANRNNLNPAQKLMLEQLESQFVLMQQHQMRPTGVAQVRSTGIPN
GPTADSSLPTNSVSGQQPQLALTRVPSVSQPGVRPACPGQPLANGPFSAGHVPCSTSRT
LGSTDTILIGNNHITGSGSNGNVPYLQRNALTLPHNRTNLTSSAEEPWKNQLSNSTQGL
HKGQSSHSAGPNGERPLSSTGPSQHLQAAGSGIQNQNGHPTLPSNSVTQ"
misc_feature 849..854
/label=SDS
misc_feature 1206..1211
/label=SDS
misc_feature 2004..2009
/label=SDS
intron 2978..3018
/label=Splicing Donor
misc_feature 3022..3055
/label=lox2272-JT1571
repeat_region 3100..3240
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 3100..3229
/label=AAV2 ITR
repeat_region 3101..3241
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
rep_origin 3315..3770
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 4052..4156
/gene="bla"
/label=AmpR promoter
CDS 4157..5017
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 5188..5776
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtgtcgac tctagaggat ctgcgatcgc tccggtgccc
181 gtcagtgggc agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca
241 attgaacggg tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact
301 ggctccgcct ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga
361 acgttctttt tcgcaacggg tttgccgcca gaacacagct gaagcttcga ggggctcgca
421 tctctccttc acgcgcccgc cgccctacct gaggccgcca tccacgccgg ttgagtcgcg
481 ttctgccgcc tcccgcctgt ggtgcctcct gaactgcgtc cgccgtctag gtaagtttaa
541 agctcaggtc gagaccgggc ctttgtccgg cgctcccttg gagcctacct agactcagcc
601 ggctctccac gctttgcctg accctgcttg ctcaactcta cgtctttgtt tcgttttctg
661 ttctgcgccg ttacagatcc aagctgtgac cggcgcctac cgtacggcca ccatgtaccc
721 ttatgacgta cctgactatg ctggatctaa atcctgcgga gtgtcgctcg ctaccgccgc
781 cgctgccgcc gccgctttcg gtgatgagga aaagaaaatg gcggcgggaa aagcgagcgg
841 cgagagcgag gaggcgtccc ccagcctgac agccgaggag agggaggcgc tcggcggact
901 ggacagccgc ctctttgggt tcgtgagatt tcatgaagat ggcgccagga cgaaggccct
961 actgggcaag gctgttcgct gctatgaatc tctaatctta aaagctgaag gaaaagtgga
1021 gtctgatttc ttttgtcaat taggtcactt caacctctta ttggaagatt atccaaaagc
1081 attatctgca taccagaggt actacagttt acagtctgac tactggaaga atgctgcctt
1141 tttatatggt cttggtttgg tctacttcca ttataatgca tttcagtggg caattaaagc
1201 atttcaggag gtgctttatg ttgatcccag cttttgtcga gccaaggaaa ttcatttacg
1261 acttgggctt atgttcaaag tgaacacaga ctatgagtct agtttaaagc attttcagtt
1321 agctttggtt gactgtaatc cctgcacttt gtccaatgct gaaattcaat ttcacattgc
1381 ccacttatat gaaacccaga ggaaatatca ttctgcaaaa gaagcttatg aacaactttt
1441 gcagacagag aatctttctg cacaagtaaa agcaactgtc ttacaacagt taggttggat
1501 gcatcacact gtagatctcc tgggagataa agccaccaag gaaagctatg ctattcagta
1561 tctccaaaag tccttggaag cagatcctaa ttctggccag tcctggtatt tcctcggaag
1621 gtgctattca agtattggga aagttcagga tgcctttata tcttacaggc agtctattga
1681 taaatcagaa gcaagtgcag atacatggtg ttcaataggt gtgctatatc agcagcaaaa
1741 tcagcccatg gatgctttac aggcctatat ttgtgctgta caattggacc atggccatgc
1801 tgcagcctgg atggacctag gcactctcta tgaatcctgc aaccagcctc aggatgccat
1861 taaatgctac ttaaatgcaa ctagaagcaa aagttgtagt aatacctctg cacttgcagc
1921 acgaattaag tatttacagg ctcagttgtg taaccttcca caaggtagtc tacagaataa
1981 aactaaatta cttcctagta ttgaggaggc gtggagccta ccaattcccg cagagcttac
2041 ctccaggcag ggtgccatga acacagcaca gcaggcatgt aaacctcatc atccaaatac
2101 tgaacctgta ttaggcctca gtcaaacacc aatttcacag caatccttgc cactacacat
2161 gattccttct agccaagtag atgacctgtc cagtcctgcc aagaggaaaa gaacatctag
2221 tccaacaaag aatacttctg acaattggag tggtggacat gctgtgtcac atcctccagt
2281 acagcaacaa gctcattcat ggtgtttgac accacagaaa ttacagcatt tggaacagct
2341 ccgcgcaaat agaaataatt taaatccagc acagaaactg atgctggaac agctggaaag
2401 tcagtttgtc ttaatgcaac aacaccaaat gagaccaaca ggagttgcac aggtacgatc
2461 tactggaatt cctaatgggc caacagctga ctcatcactg cctacaaact cagtctctgg
2521 ccagcagcca cagcttgctc tgaccagagt gcctagcgtc tctcagcctg gagtccgtcc
2581 tgcctgccct gggcagcctt tggccaatgg acccttttct gcaggccatg ttccctgtag
2641 cacatcaaga acgctgggaa gtacagacac tattttgata ggcaataatc atataacagg
2701 aagtggaagt aatggaaacg tgccttacct gcagcgaaac gcactcactc tacctcataa
2761 ccgcacaaac ctgaccagca gcgcagagga gccgtggaaa aaccaactat ctaactccac
2821 tcaggggctt cacaaaggtc agagttcaca ttcggcaggt cctaatggtg aacgacctct
2881 ctcttccact gggccttccc agcatctcca ggcagctggc tctggtattc agaatcagaa
2941 cggacatccc accctgccta gcaattcagt aacacaggta agtatcaagg ttacaagaca
3001 ggtttaagga gaccaataag ctattattcg tataggatac tttatacgaa gttattaagt
3061 tatggagacc ggtgtggcac gtgcggaccg agcggccgca ggaaccccta gtgatggagt
3121 tggccactcc ctctctgcgc gctcgctcgc tcactgaggc cgggcgacca aaggtcgccc
3181 gacgcccggg ctttgcccgg gcggcctcag tgagcgagcg agcgcgcagc tgcctgcagg
3241 ggcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatacgtc
3301 aaagcaacca tagtacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac
3361 gcgcagcgtg accgctacac ttgccagcgc cttagcgccc gctcctttcg ctttcttccc
3421 ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt
3481 agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt tgggtgatgg
3541 ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac
3601 gttctttaat agtggactct tgttccaaac tggaacaaca ctcaactcta tctcgggcta
3661 ttcttttgat ttataaggga ttttgccgat ttcggtctat tggttaaaaa atgagctgat
3721 ttaacaaaaa tttaacgcga attttaacaa aatattaacg tttacaattt tatggtgcac
3781 tctcagtaca atctgctctg atgccgcata gttaagccag ccccgacacc cgccaacacc
3841 cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac
3901 cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg
3961 aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta
4021 gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
4081 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
4141 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
4201 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
4261 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
4321 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
4381 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
4441 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
4501 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
4561 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
4621 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
4681 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga
4741 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
4801 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
4861 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
4921 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
4981 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
5041 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
5101 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
5161 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
5221 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
5281 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
5341 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
5401 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
5461 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
5521 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
5581 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
5641 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
5701 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
5761 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
5821 gccttttgct cacatgt
//
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