pAAVLINK-EFS-HA-5'-PHF3

LOCUS       pAAVLINK-EFS-HA-5'-PHF3          7181 bp DNA     circular SYN 10-AUG-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7181)
  AUTHORS   Stavrini Papadaki
  TITLE     Direct Submission
  JOURNAL   Exported Jun 9, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..7181
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
                     /note="color: #ffe4c4"
     promoter        145..356
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     CDS             371..397
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             404..4321
                     /codon_start=1
                     /gene="PHF3"
                     /product="PHD finger protein 3 isoform 1"
                     /label=PHF3
                     /note="isoform 1 is encoded by transcript variant 4"
                     /db_xref="GI:1634229891"
                     /db_xref="CCDS:CCDS4966.1"
                     /db_xref="GeneID:23469"
                     /db_xref="HGNC:HGNC:8921"
                     /db_xref="MIM:607789"
                     /protein_id="NP_001357277.1"
                     /translation="DIVDTFNHLIPTEHLDDALFLGSNLENEVCEDFSASQNVLEDSLK
                     NMLSDKDPMLGSASNQFCLPVLDSNDPNFQMPCSTVVGLDDIMDEGVVKESGNDTIDEE
                     ELILPNRNLRDKVEENSVRSPRKSPRLMAQEQVRSLRQSTIAKRSNAAPLSNTKKASGK
                     TVSTAKAGVKQPERSQVKEEVCMSLKPEYHKENRRCSRNSGQIEVVPEVSVSSSHSSVS
                     SCLEMKDEDGLDSKHKCNNPGEIDVPSHELNCSLLSETCVTIGEKKNEALMECKAKPVG
                     SPLFKFSDKEEHEQNDSISGKTGETVVEEMIATRKVEQDSKETVKLSHEDDHILEDAGS
                     SDISSDAACTNPNKTENSLVGLPSCVDEVTECNLELKDTMGIADKTENTLERNKIEPLG
                     YCEDAESNRQLESTEFNKSNLEVVDTSTFGPESNILENAICDVPDQNSKQLNAIESTKI
                     ESHETANLQDDRNSQSSSVSYLESKSVKSKHTKPVIHSKQNMTTDAPKKIVAAKYEVIH
                     SKTKVNVKSVKRNTDVPESQQNFHRPVKVRKKQIDKEPKIQSCNSGVKSVKNQAHSVLK
                     KTLQDQTLVQIFKPLTHSLSDKSHAHPGCLKEPHHPAQTGHVSHSSQKQCHKPQQQAPA
                     MKTNSHVKEELEHPGVEHFKEEDKLKLKKPEKNLQPRQRRSSKSFSLDEPPLFIPDNIA
                     TIRREGSDHSSSFESKYMWTPSKQCGFCKKPHGNRFMVGCGRCDDWFHGDCVGLSLSQA
                     QQMGEEDKEYVCVKCCAEEDKKTEILDPDTLENQATVEFHSGDKTMECEKLGLSKHTTN
                     DRTKYIDDTVKHKVKILKRESGEGRNSSDCRDNEIKKWQLAPLRKMGQPVLPRRSSEEK
                     SEKIPKESTTVTCTGEKASKPGTHEKQEMKKKKVEKGVLNVHPAASASKPSADQIRQSV
                     RHSLKDILMKRLTDSNLKVPEEKAAKVATKIEKELFSFFRDTDAKYKNKYRSLMFNLKD
                     PKNNILFKKVLKGEVTPDHLIRMSPEELASKELAAWRRRENRHTIEMIEKEQREVERRP
                     ITKITHKGEIEIESDAPMKEQEAAMEIQEPAANKSLEKPEGSEKQKEEVDSMSKDTTSQ
                     HRQHLFDLNCKICIGRMAPPVDDLSPKKVKVVVGVARKHSDNEAESIADALSSTSNILA
                     SEFFEEEKQESPKSTFSPAPRPEMPGTVEVESTFLARLNFIWKGFINMPSVAKFVTKAY
                     PVSGSPEYLTEDLPDSIQVGGRISPQTVWDYVEKIKASGTKEICVVRFTPVTEEDQISY
                     TLLFAYFSSRKRYGVAANNMKQ"
     misc_feature    3687..3692
                     /label=SDS
     misc_feature    3915..3920
                     /label=SDS
     intron          4322..4362
                     /label=Splicing Donor
     misc_feature    4366..4399
                     /label=lox2272-JT1571
     repeat_region   4444..4584
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     misc_feature    4444..4573
                     /label=AAV2 ITR
     repeat_region   4445..4585
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     rep_origin      4659..5114
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        5396..5500
                     /gene="bla"
                     /label=AmpR promoter
     CDS             5501..6361
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      6532..7120
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtgggcag agcgcacatc gcccacagtc cccgagaagt
      181 tggggggagg ggtcggcaat tgaacgggtg cctagagaag gtggcgcggg gtaaactggg
      241 aaagtgatgt cgtgtactgg ctccgccttt ttcccgaggg tgggggagaa ccgtatataa
      301 gtgcagtagt cgccgtgaac gttctttttc gcaacgggtt tgccgccaga acacagcgta
      361 cgccaccatg tacccttatg acgtacctga ctatgctgga tctgatatag ttgatacatt
      421 taatcattta attcctactg aacacttaga tgatgcccta tttctaggat ccaacctgga
      481 gaatgaagtc tgtgaggatt ttagtgcaag tcaaaatgtc ttagaggact cgctgaagaa
      541 catgctcagc gataaggatc ctatgctagg atctgcaagt aaccagttct gtttgcctgt
      601 tttggatagc aatgatccca atttccagat gccttgttca acagttgttg gtcttgacga
      661 tattatggat gaaggagttg ttaaagaaag tggcaatgat accattgatg aagaagaact
      721 gattttacct aacaggaact taagggacaa ggtagaagaa aattcagtga gatctccaag
      781 aaaatcacct cgtttaatgg cacaagaaca agtaagaagt ttgcgacaga gcactattgc
      841 caagcgttca aatgcagcac cattaagtaa cacaaaaaaa gcatctggga agactgtatc
      901 tactgctaaa gcaggagtga aacaaccaga aaggagtcag gttaaagaag aagtatgtat
      961 gtcactgaaa cctgagtacc ataaggagaa tagaaggtgc agccgaaata gcggacaaat
     1021 tgaagtggta cctgaagtat cagtgtcttc aagtcattct tcagtgtcat cttgtcttga
     1081 aatgaaggat gaagatggat tagattctaa gcataagtgt aataatccgg gagaaataga
     1141 tgtgccatct catgaattaa attgttcact tctttcagag acttgtgtta ctattggaga
     1201 aaagaaaaat gaagctttga tggaatgtaa agccaagcct gttggtagtc cattgtttaa
     1261 gttttcagat aaagaagaac atgaacaaaa tgattccatt tcaggtaaaa cgggtgagac
     1321 tgttgttgaa gaaatgatag caacaagaaa agttgaacaa gattcaaagg agacagtaaa
     1381 attatcccat gaagatgacc atattcttga ggacgctgga tcttctgata tttctagtga
     1441 tgctgcttgt acaaatccaa ataagacaga aaacagcctt gtaggtttgc ctagttgtgt
     1501 agatgaagtg actgaatgta atttggaatt gaaggatacc atgggtattg ctgataaaac
     1561 tgagaacacc cttgaaagaa ataaaattga accgttgggt tattgtgaag atgcggagtc
     1621 taataggcag ttggagagca ctgagtttaa taaatcaaac ttagaggtgg ttgatactag
     1681 tacttttgga ccggaaagta atatcttgga aaatgctatt tgtgatgtgc ctgaccaaaa
     1741 ttcaaaacag ttgaatgcta tagaaagtac taaaatagag tcccatgaaa cagcaaacct
     1801 tcaggatgac agaaacagcc agtcaagtag cgtttcttac ttagagtcaa aaagtgtaaa
     1861 atccaaacat acaaaacctg taattcattc taagcaaaac atgaccacag atgctccgaa
     1921 gaaaattgtt gcagcaaagt atgaagtaat acatagcaaa actaaagtta atgtcaaaag
     1981 tgtgaaacga aatactgatg taccagaatc tcagcaaaat tttcataggc cagtcaaagt
     2041 cagaaaaaaa caaattgata aggagccaaa gattcagagt tgcaattctg gggttaaatc
     2101 tgtgaaaaac caagctcatt ctgtactgaa aaaaacatta caggatcaaa ctttagtaca
     2161 aattttcaag cccttaactc attctttgag tgataagtca cacgctcatc ctggttgctt
     2221 gaaagaacct catcatcctg cacaaactgg acatgtatca cattctagcc agaaacagtg
     2281 tcataagcct cagcaacagg ccccagcaat gaaaaccaat agtcacgtga aggaagagct
     2341 tgaacaccca ggcgttgagc attttaagga agaggataaa ctgaaactga aaaaacctga
     2401 gaagaaccta caaccccgcc aaagaagaag cagcaaaagt ttttctttag atgagccacc
     2461 attgttcatt ccagataaca tagctaccat aagaagagaa ggctctgatc atagctcctc
     2521 atttgaaagc aaatatatgt ggactcccag caagcagtgt gggttttgca aaaaaccaca
     2581 tggcaacagg tttatggttg gctgtgggag atgtgatgac tggtttcatg gtgattgtgt
     2641 tgggttaagt ctttctcaag cacagcagat gggcgaggaa gacaaagaat atgtctgtgt
     2701 aaaatgttgt gctgaagaag acaaaaagac tgaaatacta gatccagata ctttggaaaa
     2761 ccaagctaca gttgaattcc atagtggaga taaaacaatg gagtgtgaaa agcttggatt
     2821 atcaaaacac acaacaaatg atagaaccaa atatatagat gatacagtga agcacaaggt
     2881 caaaatttta aaacgggagt ctggtgaagg cagaaattca tcagactgta gagataatga
     2941 aattaaaaaa tggcagctag ctcctcttcg taagatggga caaccagttt tacctcggag
     3001 atcctcagaa gaaaaaagtg aaaaaatacc gaaagagtct acaactgtta cttgcacagg
     3061 agaaaaagct tcaaaaccag gtactcatga gaagcaagag atgaaaaaga agaaagttga
     3121 aaaaggagtg cttaatgtac atcctgctgc ttctgcttcc aagccttctg cagatcagat
     3181 caggcaaagt gtcagacatt ctctcaaaga cattcttatg aagagactta cagactcaaa
     3241 tttgaaggta ccagaggaaa aggcagcaaa agttgccaca aaaattgaga aagagctttt
     3301 ctcttttttt cgggacacag atgctaaata taagaacaaa tatagaagtt tgatgtttaa
     3361 tttgaaagat cctaaaaaca atatattatt taaaaaagta ctgaaaggag aagtaactcc
     3421 tgatcatctt atcagaatga gtccagaaga actagcttct aaagagttag ctgcttggag
     3481 acgaagagaa aacagacata ccatagaaat gattgagaaa gagcagagag aagtggaacg
     3541 acggccaatc accaaaataa ctcataaagg tgaaatagaa attgagagtg atgccccaat
     3601 gaaagaacag gaagcagcca tggagattca ggaaccagcc gccaataagt cattggagaa
     3661 gccagaagga tctgaaaaac aaaaagagga ggttgactct atgtctaaag ataccactag
     3721 tcaacacaga cagcatcttt ttgatctcaa ctgcaaaatc tgcataggtc gaatggcacc
     3781 acctgtagat gatctttctc caaaaaaagt aaaagttgtt gtaggagtag ctcgcaaaca
     3841 ttcagacaat gaagcagaaa gtatagcaga tgcattatct tcaacctcaa atattttggc
     3901 ttctgaattc tttgaggagg agaaacagga gtctccaaag tcaacgttct ctcctgctcc
     3961 acgtccagag atgcctggaa ctgttgaagt tgagtctacc tttctggctc gattgaactt
     4021 catctggaaa ggttttatca acatgccttc tgtggcaaaa tttgttacca aagcctatcc
     4081 agtatctggc tccccagaat acctgacaga ggacctacca gatagtattc aagtaggtgg
     4141 caggatatca cctcagacag tttgggatta tgtggaaaaa ataaaagcat caggaaccaa
     4201 ggaaatttgt gtggttcgct tcacaccagt aactgaagaa gatcaaattt cttatacttt
     4261 gctctttgca tacttcagta gcagaaagcg ctatggagta gctgctaaca acatgaagca
     4321 ggtaagtatc aaggttacaa gacaggttta aggagaccaa taagctatta ttcgtatagg
     4381 atactttata cgaagttatt aagttatgga gaccggtgtg gcacgtgcgg accgagcggc
     4441 cgcaggaacc cctagtgatg gagttggcca ctccctctct gcgcgctcgc tcgctcactg
     4501 aggccgggcg accaaaggtc gcccgacgcc cgggctttgc ccgggcggcc tcagtgagcg
     4561 agcgagcgcg cagctgcctg caggggcgcc tgatgcggta ttttctcctt acgcatctgt
     4621 gcggtatttc acaccgcata cgtcaaagca accatagtac gcgccctgta gcggcgcatt
     4681 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccttagc
     4741 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca
     4801 agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc
     4861 caaaaaactt gatttgggtg atggttcacg tagtgggcca tcgccctgat agacggtttt
     4921 tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac
     4981 aacactcaac tctatctcgg gctattcttt tgatttataa gggattttgc cgatttcggt
     5041 ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt
     5101 aacgtttaca attttatggt gcactctcag tacaatctgc tctgatgccg catagttaag
     5161 ccagccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc
     5221 atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc
     5281 gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt tataggttaa
     5341 tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg
     5401 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
     5461 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg
     5521 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
     5581 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact
     5641 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat
     5701 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga
     5761 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac
     5821 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat
     5881 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac
     5941 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct
     6001 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac
     6061 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga
     6121 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg
     6181 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact
     6241 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac
     6301 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta
     6361 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
     6421 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
     6481 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     6541 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     6601 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     6661 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     6721 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     6781 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     6841 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     6901 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     6961 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     7021 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     7081 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     7141 tttacggttc ctggcctttt gctggccttt tgctcacatg t
//