pAAVLINKv1-pCALM1-Cre-3'-PLXNA3-FLAG

LOCUS      pAAVLINKv1-pCALM1-Cre-3'-PLXNA3-FLAG            6940 bp DNA     circular SYN 25-NOV-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6940)
  AUTHORS   Stavrini Papadaki
  TITLE     Direct Submission
  JOURNAL   Exported Jun 12, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..6940
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
                     /note="color: #ffe4c4"
     promoter        145..265
                     /label=pCALM1
     CDS             277..1308
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
                     LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
                     PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
                     LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
                     RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
                     RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
                     DGD"
     intron          1309..1349
                     /label=Splicing Donor
     protein_bind    1356..1389
                     /label=lox2272-JTZ17
                     /bound_moiety="Cre recombinase"
                     /note="Right element (RE) mutant of loxP (Araki et al., 
                     2010). Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     intron          1390..1481
                     /label=Splicing Acceptor
                     /note="Splicing acceptor"
     CDS             1482..3653
                     /codon_start=1
                     /gene="PLXNA3"
                     /gene_synonym="6.3; HSSEXGENE; PLXN3; PLXN4; XAP-6"
                     /product="plexin-A3 precursor"
                     /label=PLXNA3
                     /note="Sex chromosome X transmembrane protein of 
                     HGFreceptor family 3; plexin-4; semaphorin receptor SEX"
                     /db_xref="GI:533112479"
                     /db_xref="CCDS:CCDS14752.1"
                     /db_xref="GeneID:55558"
                     /db_xref="HGNC:HGNC:9101"
                     /db_xref="MIM:300022"
                     /protein_id="NP_059984.3"
                     /translation="GKNLIPAAAGSSRLNYTVLIGGQPCSLTVSDTQLLCDSPSQTGRQ
                     PVMVLVGGLEFWLGTLHISAERALTLPAMMGLAAGGGLLLLAITAVLVAYKRKTQDADR
                     TLKRLQLQMDNLESRVALECKEAFAELQTDINELTNHMDEVQIPFLDYRTYAVRVLFPG
                     IEAHPVLKELDTPPNVEKALRLFGQLLHSRAFVLTFIHTLEAQSSFSMRDRGTVASLTM
                     VALQSRLDYATGLLKQLLADLIEKNLESKNHPKLLLRRTESVAEKMLTNWFTFLLHKFL
                     KECAGEPLFLLYCAIKQQMEKGPIDAITGEARYSLSEDKLIRQQIDYKTLTLHCVCPEN
                     EGSAQVPVKVLNCDSITQAKDKLLDTVYKGIPYSQRPKAEDMDLEWRQGRMTRIILQDE
                     DVTTKIECDWKRLNSLAHYQVTDGSLVALVPKQVSAYNMANSFTFTRSLSRYESLLRTA
                     SSPDSLRSRAPMITPDQETGTKLWHLVKNHDHADHREGDRGSKMVSEIYLTRLLATKGT
                     LQKFVDDLFETVFSTAHRGSALPLAIKYMFDFLDEQADQRQISDPDVRHTWKSNCLPLR
                     FWVNVIKNPQFVFDIHKNSITDACLSVVAQTFMDSCSTSEHRLGKDSPSNKLLYAKDIP
                     NYKSWVERYYRDIAKMASISDQDMDAYLVEQSRLHASDFSVLSALNELYFYVTKYRQEI
                     LTALDRDASCRKHKLRQKLEQIISLVSSDS"
     misc_feature    1541..1546
                     /label=SDS
     regulatory      2143..2152
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3660..3725
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     terminator      3729..4160
                     /label=W3SL
                     /note="Short WPRE and SV40 polyA"
     misc_feature    3873..3878
                     /label=SDS
     repeat_region   4203..4343
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     misc_feature    4203..4332
                     /label=AAV2 ITR
     repeat_region   4204..4344
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     rep_origin      4418..4873
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        5155..5259
                     /gene="bla"
                     /label=AmpR promoter
     CDS             5260..6120
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      6291..6879
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtcgctct gcgcgaggcg gctccgccgc ggcggaggga
      181 tacggcgcac catatatata tcgcggggcg cagactcgcg ctccggcagt ggtgctggga
      241 gtgtcgtgga cgccgtgccg ttactcgtac gccaccatgt ccaatttact gaccgtacac
      301 caaaatttgc ctgcattacc ggtcgatgca acgagtgatg aggttcgcaa gaacctgatg
      361 gacatgttca gggatcgcca ggcgttttct gagcatacct ggaaaatgct tctgtccgtt
      421 tgccggtcgt gggcggcatg gtgcaagttg aataaccgga aatggtttcc cgcagaacct
      481 gaagatgttc gcgattatct tctatatctt caggcgcgcg gtctggcagt aaaaaccatc
      541 cagcaacatt tgggccagct aaacatgctt catcgtcggt ccgggctgcc acgaccaagt
      601 gacagcaatg ctgtttcact ggttatgcgg cggatccgaa aagaaaacgt tgatgccggt
      661 gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt tcgaccaggt tcgttcactc
      721 atggaaaata gcgatcgctg ccaggatata cgtaatctgg catttctggg gattgcttat
      781 aacaccctgt tacgtatagc cgaaattgcc aggatcaggg ttaaagatat ctcacgtact
      841 gacggtggga gaatgttaat ccatattggc agaacgaaaa cgctggttag caccgcaggt
      901 gtagagaagg cacttagcct gggggtaact aaactggtcg agcgatggat ttccgtctct
      961 ggtgtagctg atgatccgaa taactacctg ttttgccggg tcagaaaaaa tggtgttgcc
     1021 gcgccatctg ccaccagcca gctatcaact cgcgccctgg aagggatttt tgaagcaact
     1081 catcgattga tttacggcgc taaggatgac tctggtcaga gatacctggc ctggtctgga
     1141 cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg ctggagtttc aataccggag
     1201 atcatgcaag ctggtggctg gaccaatgta aatattgtaa tgaactatat ccgtaacctg
     1261 gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg gcgattaggt aagtatcaag
     1321 gttacaagac aggtttaagg agaccaataa ctagtataac ttcgtatagg atactttata
     1381 gcaatttatg aaactgggct tgtcgagaca gagaagactc ttgcgtttct gataggcacc
     1441 tattggtctt actgacatcc actttgcctt tctctccaca gggcaagaac ctgattcccg
     1501 cggcagccgg cagctcccgc ctcaactaca ctgtgctgat aggaggccag ccgtgttcgc
     1561 tcactgtctc ggacacacaa ctcctgtgcg actcacccag ccagactggc cggcagcctg
     1621 tcatggtgct ggtgggtggc ctggagttct ggctgggcac cctgcacatc tcggcagagc
     1681 gggcgctgac cctaccggcc atgatggggc tggcggcggg gggtgggctc ctgctgctgg
     1741 ccatcacagc cgtgctggtg gcgtacaagc gcaagactca ggacgcggac cgtaccctca
     1801 agcgtctgca gctgcagatg gacaacctgg agtcccgtgt ggccctggag tgcaaggaag
     1861 cttttgcaga gctgcagacg gacatcaatg agctgactaa ccacatggac gaggtgcaga
     1921 tccccttcct ggactaccgg acttacgccg tgcgcgtgct cttcccgggc atcgaggccc
     1981 acccggtgct caaggagctg gatacgccac ccaacgtgga gaaggccctg cgcctcttcg
     2041 ggcagctgct gcacagccgc gcgttcgtgc ttaccttcat ccacacgctg gaggcccaga
     2101 gcagcttctc catgcgcgac cgcggcaccg tggcctcgct caccatggtg gccctgcaga
     2161 gccggctcga ctatgccacg gggctgctca agcaactgct ggccgacctc atcgagaaga
     2221 acctcgagag caagaaccac cccaagctgc tgctacgcag gacagagtca gtggctgaga
     2281 agatgcttac caactggttc acgttcctgc tgcataagtt tctgaaggag tgtgctgggg
     2341 agcctctctt cctgctttac tgtgccatca agcagcagat ggagaagggc cccattgatg
     2401 ccatcacggg cgaggcacga tactccctga gcgaggacaa gctcatccgt cagcagatcg
     2461 actacaagac actgaccctt cactgcgtgt gtccggagaa cgagggcagc gcccaggtcc
     2521 cagtgaaggt tctcaactgt gacagcatca cccaggccaa agataagctg ctggacactg
     2581 tgtacaaggg cattccgtac tcccagcgtc ccaaagctga ggacatggac ctggagtggc
     2641 gccagggccg catgactcgc atcatcctcc aggatgagga tgtcaccacc aagatcgagt
     2701 gtgactggaa gaggctcaac tcactggccc actaccaggt gacagacggt tccttggtgg
     2761 cattggtgcc caaacaagtg tctgcctata acatggccaa ctccttcacc ttcacccgct
     2821 ccctcagccg ctacgagagc ttgctccgca cggccagcag ccctgatagc ctccgctcac
     2881 gggcacccat gattacgcct gaccaggaga caggcaccaa attgtggcac ctggtgaaaa
     2941 accacgacca tgccgaccat cgcgaggggg accgtggcag caagatggtc tccgagatct
     3001 acctgacacg gctgctggcc accaagggca cactgcagaa gttcgtggat gacctctttg
     3061 agacagtgtt cagcacagcc caccggggct cggccctgcc cctggccatc aagtacatgt
     3121 tcgacttcct ggatgagcag gcggaccagc gccagatcag cgaccccgat gtgcgccaca
     3181 cctggaagag caactgcctg ccgctgcgct tctgggtgaa tgtgatcaag aacccgcagt
     3241 tcgtgttcga catccacaag aacagcatca cggatgcctg cctgtcggtg gtagcccaga
     3301 ccttcatgga ctcctgctct acatccgagc accgcctggg gaaggactcg ccctccaaca
     3361 aactgctcta cgccaaggac atccccaact acaagagctg ggtggagagg tattatcgag
     3421 acattgcaaa gatggcatcc atcagcgacc aggacatgga tgcctacctg gtggagcagt
     3481 cccgcctcca cgccagcgac ttcagcgtcc tgagtgcgct caacgagctg tatttctatg
     3541 tcaccaagta ccgccaggag attctcacgg ctctggaccg agatgcctct tgtcggaagc
     3601 ataagttgcg gcagaaactg gaacagatca tcagcctcgt gtccagcgac agctgtacag
     3661 actacaaaga ccatgacggt gattataaag atcatgacat cgactacaag gatgacgatg
     3721 acaagtaact cgagataatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat
     3781 tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca
     3841 tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttagttct
     3901 tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt
     3961 gggcactgac aattccgtgg tgtttatttg tgaaatttgt gatgctattg ctttatttgt
     4021 aaccatctag ctttatttgt gaaatttgtg atgctattgc tttatttgta accattataa
     4081 gctgcaataa acaagttaac aacaacaatt gcattcattt tatgtttcag gttcaggggg
     4141 agatgtggga ggttttttaa agatctggag accggtgtgg cacgtgcgga ccgagcggcc
     4201 gcaggaaccc ctagtgatgg agttggccac tccctctctg cgcgctcgct cgctcactga
     4261 ggccgggcga ccaaaggtcg cccgacgccc gggctttgcc cgggcggcct cagtgagcga
     4321 gcgagcgcgc agctgcctgc aggggcgcct gatgcggtat tttctcctta cgcatctgtg
     4381 cggtatttca caccgcatac gtcaaagcaa ccatagtacg cgccctgtag cggcgcatta
     4441 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccttagcg
     4501 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
     4561 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
     4621 aaaaaacttg atttgggtga tggttcacgt agtgggccat cgccctgata gacggttttt
     4681 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
     4741 acactcaact ctatctcggg ctattctttt gatttataag ggattttgcc gatttcggtc
     4801 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta
     4861 acgtttacaa ttttatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc
     4921 cagccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca
     4981 tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg
     5041 tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac gcctattttt ataggttaat
     5101 gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga
     5161 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
     5221 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt
     5281 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
     5341 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
     5401 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
     5461 agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag
     5521 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
     5581 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
     5641 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
     5701 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
     5761 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg
     5821 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac
     5881 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg
     5941 tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg
     6001 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact
     6061 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
     6121 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
     6181 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
     6241 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     6301 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     6361 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     6421 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
     6481 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     6541 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     6601 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     6661 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     6721 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     6781 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     6841 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     6901 ttacggttcc tggccttttg ctggcctttt gctcacatgt
//