PCDH-CMV-Hotag3-FKBP-mCherry-TRIM6 L148P

LOCUS       PCDH-CMV-Hotag3-FKBP-mCherry-TRIM6 L148P            9105 bp DNA     circular SYN 04-JUL-2025
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9105)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 13, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..9105
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             234..414
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /note="color: #ffe4c4"
     misc_feature    458..583
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
                     /note="color: #ffe4c4"
     misc_feature    1076..1309
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
                     /note="color: #ffe4c4"
     CDS             1493..1537
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /note="color: #cc99b2"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1798..1915
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
                     /note="color: #ffe4c4"
     enhancer        1938..2241
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
                     /note="color: #ffffff"
     promoter        2242..2445
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
                     /note="color: #ffffff; direction: RIGHT"
     regulatory      2490..2499
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /note="color: #a6acb3"
                     /regulatory_class="other"
     CDS             2625..2948
                     /codon_start=1
                     /product="human FK506-binding protein FKBP12"
                     /label=FKBP
                     /note="This feature has 2 segments:
                        1: 2625 .. 2627 / #993366
                        2: 2628 .. 2948 / #993366"
                     /translation="MGVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNK
                     PFKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVEL
                     LKLE"
     CDS             2985..3689
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /note="color: #ff0000"
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     misc_feature    3726..5192
                     /label=Feature 25
                     /note="color: #a6acb3"
     misc_feature    4168
                     /label=l148p
                     /note="color: #a6acb3"
     misc_feature    5202..5789
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
                     /note="color: #ffffff"
     CDS             complement(5673..5684)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /note="color: #cc99b2
                     Cleavage site after base 5672"
                     /translation="IEGR"
     LTR             5862..6095
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
                     /note="color: #ffe4c4"
     polyA_signal    6167..6288
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
                     /note="color: #a6acb3"
     rep_origin      6307..6442
                     /label=SV40 ori
                     /note="SV40 origin of replication"
                     /note="color: #ffff00"
     primer_bind     complement(6480..6496)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: LEFT"
     protein_bind    6504..6520
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="color: #31849b"
     promoter        complement(6528..6558)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
                     /note="This reverse directional feature has 3 segments:
                        1: 6528 .. 6534 / #ffffff / -10
                        2: 6535 .. 6552 / #ffffff
                        3: 6553 .. 6558 / #ffffff / -35"
     protein_bind    6573..6594
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
                     /note="color: #31849b"
     rep_origin      complement(6882..7470)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     CDS             complement(7641..8501)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1: 7641 .. 8432 / #ccffcc
                        2: 8433 .. 8501 / #ccffcc / signal sequence
                     Cleavage site after base 8432"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(8502..8606)
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: LEFT"
     primer_bind     9080..9096
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: RIGHT"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggttaacttt
     1801 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc
     1861 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga
     1921 tactagttgg agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc
     1981 aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg
     2041 actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat
     2101 caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc
     2161 tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta catctacgta
     2221 ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag
     2281 cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt
     2341 tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa
     2401 atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt
     2461 cagattctag accgctagcg ctaccggtcg ccaccatggg cgagatcgcc aagagcctga
     2521 aggagatcgc caaatccctg aaggagattg cctggtccct gaaggaaatc gccaagtccc
     2581 tgaagggcgg cggctccgga ggctctggag gatctggcgg cagcatggga gtgcaggtgg
     2641 aaaccatctc cccaggagac gggcgcacct tccccaagcg cggccagacc tgcgtggtgc
     2701 actacaccgg gatgcttgaa gatggaaaga aatttgattc ctcccgggac agaaacaagc
     2761 cctttaagtt tatgctaggc aagcaggagg tgatccgagg ctgggaagaa ggggttgccc
     2821 agatgagtgt gggtcagaga gccaaactga ctatatctcc agattatgcc tatggtgcca
     2881 ctgggcaccc aggcatcatc ccaccacatg ccactctcgt cttcgatgtg gagcttctaa
     2941 aactggaagg cggaagtgga ggatctggag gttcaggtgg ttctgtgagc aagggcgagg
     3001 aggataacat ggccatcatc aaggagttca tgcgcttcaa ggtgcacatg gagggctccg
     3061 tgaacggcca cgagttcgag atcgagggcg agggcgaggg ccgcccctac gagggcaccc
     3121 agaccgccaa gctgaaggtg accaagggtg gccccctgcc cttcgcctgg gacatcctgt
     3181 cccctcagtt catgtacggc tccaaggcct acgtgaagca ccccgccgac atccccgact
     3241 acttgaagct gtccttcccc gagggcttca agtgggagcg cgtgatgaac ttcgaggacg
     3301 gcggcgtggt gaccgtgacc caggactcct ccctgcagga cggcgagttc atctacaagg
     3361 tgaagctgcg cggcaccaac ttcccctccg acggccccgt aatgcagaag aagaccatgg
     3421 gctgggaggc ctcctccgag cggatgtacc ccgaggacgg cgccctgaag ggcgagatca
     3481 agcagaggct gaagctgaag gacggcggcc actacgacgc tgaggtcaag accacctaca
     3541 aggccaagaa gcccgtgcag ctgcccggcg cctacaacgt caacatcaag ttggacatca
     3601 cctcccacaa cgaggactac accatcgtgg aacagtacga acgcgccgag ggccgccact
     3661 ccaccggcgg catggacgag ctgtacaagt ccggactcag atctcgagct caagcttcgg
     3721 aattcatgac ttcaccagta ctggtggaca tacgagaaga ggtgacctgc cctatctgcc
     3781 tggagctcct aacagaaccc ctgagcatag actgtggcca cagcttctgc caagcctgca
     3841 tcacaccaaa tggcagggaa tcagtgattg gtcaagaagg ggaaagaagc tgccctgtgt
     3901 gccagaccag ctaccagcca gggaacctgc ggcctaatcg gcatctggcc aacatagtga
     3961 ggcggctcag agaggtagtg ttgggccctg ggaagcagct gaaagcagtt ctttgtgcag
     4021 accatggaga aaaactgcag ctcttctgtc aggaggatgg gaaggtcatt tgctggcttt
     4081 gtgagcggtc tcaggagcac cgtggtcacc acacgttcct cgtggaggag gttgcccagg
     4141 agtaccagga gaagtttcag gagtctccaa agaagctgaa gaacgaggag caggaagctg
     4201 agaagctaac agcttttatc agagagaaga aaacatcctg gaagaatcag atggagcctg
     4261 agagatgcag gatccagaca gagtttaatc agctgcgaaa tatcctagac agagtggagc
     4321 aacgggagct gaaaaagctg gaacaggaag agaagaaggg gctacgaatt atagaagagg
     4381 ctgagaatga tctggtccac cagacccagt cgctgcgaga gctcatctcg gatctggagc
     4441 gtcgatgtca ggggtcaaca atggagctgc tgcaggatgt gagtgatgtc acagaaagga
     4501 gtgagttctg gaccctgagg aagccagaag ctctccctac aaagctgaga agtatgttcc
     4561 gagccccaga tctgaaaagg atgctgcgag tgtgtagaga gctgacagat gtccaaagct
     4621 actgggttga cgtgaccctg aatccacaca cagctaattt aaatcttgtc ctggctaaaa
     4681 accggagaca agtgaggttt gtgggagcta aagtatctgg accttcctgt ctggaaaagc
     4741 attatgactg tagtgtcctg ggctcccagc acttctcctc tggtaagcat tactgggagg
     4801 tagatgtggc caagaagact gcctggatcc tgggggtatg cagcaattca ctgggaccta
     4861 cattctcttt caaccatttt gctcaaaatc acagtgctta ctccaggtat cagcctcaga
     4921 gtggatactg ggtgattggg ttacagcata accatgaata tagggcctat gaggattctt
     4981 ccccttccct gcttctctcc atgacagtgc cccctcgccg tgttggggtt ttcttagatt
     5041 atgaggctgg tactgtctcc ttttataatg tcacaaacca tggcttcccc atctacactt
     5101 tctctaaata ttactttccc actactcttt gtccatattt taatccttgc aactgtgtaa
     5161 ttcctatgac cctgcgtcgt ccaagctctt gatagggatc caatcaacct ctggattaca
     5221 aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat
     5281 acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct
     5341 ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac
     5401 gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca
     5461 cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca
     5521 tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg
     5581 tggtgttgtc ggggaagctg acgtcctttc catggctgct cgcctgtgtt gccacctgga
     5641 ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt
     5701 cccgcggcct gctgccggct ctgcggcctc ttccgcgtct ccgccttcgc cctcagacga
     5761 gtcggatctc cctttggccg cctccccgcc ggtaccttta agaccaatga cttacaaggc
     5821 agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc
     5881 ccaacgaaaa taagatctgc tttttgcttg tactgggtct ctctggttag accagatctg
     5941 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
     6001 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
     6061 cagacccttt tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc atcttattat
     6121 tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggaact tgtttattgc
     6181 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
     6241 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggct
     6301 ctagctatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta
     6361 atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag
     6421 tgaggaggct tttttggagg cctagacttt tgcagagacg gcccaaattc gtaatcatgg
     6481 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc
     6541 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg
     6601 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc
     6661 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact
     6721 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
     6781 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
     6841 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     6901 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     6961 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     7021 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     7081 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     7141 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     7201 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     7261 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     7321 aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     7381 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     7441 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     7501 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     7561 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     7621 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
     7681 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
     7741 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
     7801 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
     7861 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
     7921 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
     7981 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
     8041 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
     8101 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
     8161 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
     8221 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
     8281 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
     8341 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
     8401 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
     8461 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
     8521 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
     8581 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa
     8641 gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt
     8701 ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc
     8761 acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt
     8821 gttggcgggt gtcggggctg gcttaactat gcggcatcag agcagattgt actgagagtg
     8881 caccatatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggcgc
     8941 cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta
     9001 ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt aacgccaggg
     9061 ttttcccagt cacgacgttg taaaacgacg gccagtgcca agctg
//