| 质粒名称: | pCMV-BE3 |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 73021 |
| 存储实验室: | David Liu |
|
载体骨架基本信息 |
|
| 载体名称: | CMV |
| 载体出品公司: | origene |
| 载体抗性: | 氨苄西林 |
| 空载体大小: | 3399 bp |
| 完整质粒大小: | 8532bp |
| 载体类型: | 哺乳细胞表达 |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | BE3 |
| 备用名称： | rAPOBEC1-XTEN-Cas9n-UGI-NLS |
| 物种来源: | 褐家鼠（大鼠）；化脓性链球菌 |
| 插入大小: | 5133 bp |
| 启动子 | CMV |
| 克隆方法: | 结扎独立克隆 |
| 5' 测序引物 | CMV |
| 3' 测序引物 | TGGTTCTTTCCGCCTCAGAAGC |
|
文献引用方法 |
|
| 材料和方法部分: | pCMV-BE3 was a gift from David Liu (Addgene plasmid # 73021 ; http://n2t.net/addgene:73021 ; RRID:Addgene_73021) |
| 参考文献部分: | Programmable editing of a target base in genomic DNA without double-stranded DNA cleavage. Komor AC, Kim YB, Packer MS, Zuris JA, Liu DR. Nature. 2016 Apr 20. doi: 10.1038/nature17946. 10.1038/nature17946 PubMed 27096365 |
买家导航
pCMV-BE3载体质粒基本信息
pCMV-BE3质粒图谱载体图谱和pCMV-BE3载体序列质粒序列多克隆位点信息
pCMV-BE3质粒载体简介
pCMV-BE3质粒序列载体序列
LOCUS pCMV-BE3 8532 bp ds-DNA circular SYN 17-MAY-2019
DEFINITION Expresses BE3 in mammalian cells.
ACCESSION .
VERSION .
KEYWORDS pCMV-BE3
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8532)
AUTHORS Komor AC, Kim YB, Packer MS, Zuris JA, Liu DR
TITLE Programmable editing of a target base in genomic DNA without
double-stranded DNA cleavage.
JOURNAL Nature. 2016 Apr 20. doi: 10.1038/nature17946.
PUBMED 27096365
REFERENCE 2 (bases 1 to 8532)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 17, 2019 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..8532
/organism="synthetic DNA construct"
/mol_type="other DNA"
enhancer 148..527
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 528..731
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 682..702
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
primer_bind 773..792
/label=T7
/note="T7 promoter, forward primer"
promoter 773..791
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
CDS 805..1491
/codon_start=1
/product="cytidine deaminase (C to U editing enzyme) from
rat"
/label=APOBEC-1
/note="can use ssDNA as a substrate (Komor et al., 2016)"
/translation="MSSETGPVAVDPTLRRRIEPHEFEVFFDPRELRKETCLLYEINWG
GRHSIWRHTSQNTNKHVEVNFIEKFTTERYFCPNTRCSITWFLSWSPCGECSRAITEFL
SRYPHVTLFIYIARLYHHADPRNRQGLRDLISSGVTIQIMTEQESGYCWRNFVNYSPSN
EAHWPRYPHLWVRLYVLELYCIILGLPPCLNILRRKQPQLTFFTIALQSCHYQRLPPHI
LWATGLK"
CDS 1540..5640
/codon_start=1
/product="nickase mutant of the Cas9 endonuclease from the
Streptococcus pyogenes Type II CRISPR/Cas system"
/label=Cas9(D10A)
/note="generates RNA-guided single strand nicks in DNA due
to the D10A mutation in the RuvC catalytic domain"
/translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
ATLIHQSITGLYETRIDLSQLGGD"
CDS 5653..5901
/codon_start=1
/product="uracil-DNA glycosylase inhibitor from a Bacillus
subtilis bacteriophage (Mol et al., 1995)"
/label=UGI
/translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
CDS 5914..5934
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
CDS 5943..5960
/codon_start=1
/product="6xHis affinity tag"
/label=6xHis
/translation="HHHHHH"
primer_bind complement(5983..6000)
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
polyA_signal 5989..6213
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
primer_bind complement(6284..6300)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(6284..6300)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(6297..6319)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 6308..6324
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(6332..6362)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 6377..6398
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(6515..6532)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(6686..7274)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(6766..6785)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(7445..8305)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 8068..8087
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(8306..8410)
/gene="bla"
/label=AmpR promoter
primer_bind complement(8489..8508)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
ORIGIN
1 ctgcttgtgt gttggaggtc gctgagtagt gcgcgagcaa aatttaagct acaacaaggc
61 aaggcttgac cgacaattgc atgaagaatc tgcttagggt taggcgtttt gcgctgcttc
121 gcgatgtacg ggccagatat acgcgttgac attgattatt gactagttat taatagtaat
181 caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg
241 taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt
301 atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac
361 ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg
421 acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact
481 ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt
541 ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc
601 ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc
661 gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata
721 taagcagagc tggtttagtg aaccgtcaga tccgctagag atccgcggcc gctaatacga
781 ctcactatag ggagagccgc caccatgagc tcagagactg gcccagtggc tgtggacccc
841 acattgagac ggcggatcga gccccatgag tttgaggtat tcttcgatcc gagagagctc
901 cgcaaggaga cctgcctgct ttacgaaatt aattgggggg gccggcactc catttggcga
961 catacatcac agaacactaa caagcacgtc gaagtcaact tcatcgagaa gttcacgaca
1021 gaaagatatt tctgtccgaa cacaaggtgc agcattacct ggtttctcag ctggagccca
1081 tgcggcgaat gtagtagggc catcactgaa ttcctgtcaa ggtatcccca cgtcactctg
1141 tttatttaca tcgcaaggct gtaccaccac gctgaccccc gcaatcgaca aggcctgcgg
1201 gatttgatct cttcaggtgt gactatccaa attatgactg agcaggagtc aggatactgc
1261 tggagaaact ttgtgaatta tagcccgagt aatgaagccc actggcctag gtatccccat
1321 ctgtgggtac gactgtacgt tcttgaactg tactgcatca tactgggcct gcctccttgt
1381 ctcaacattc tgagaaggaa gcagccacag ctgacattct ttaccatcgc tcttcagtct
1441 tgtcattacc agcgactgcc cccacacatt ctctgggcca ccgggttgaa aagcggcagc
1501 gagactcccg ggacctcaga gtccgccaca cccgaaagtg ataaaaagta ttctattggt
1561 ttagccatcg gcactaattc cgttggatgg gctgtcataa ccgatgaata caaagtacct
1621 tcaaagaaat ttaaggtgtt ggggaacaca gaccgtcatt cgattaaaaa gaatcttatc
1681 ggtgccctcc tattcgatag tggcgaaacg gcagaggcga ctcgcctgaa acgaaccgct
1741 cggagaaggt atacacgtcg caagaaccga atatgttact tacaagaaat ttttagcaat
1801 gagatggcca aagttgacga ttctttcttt caccgtttgg aagagtcctt ccttgtcgaa
1861 gaggacaaga aacatgaacg gcaccccatc tttggaaaca tagtagatga ggtggcatat
1921 catgaaaagt acccaacgat ttatcacctc agaaaaaagc tagttgactc aactgataaa
1981 gcggacctga ggttaatcta cttggctctt gcccatatga taaagttccg tgggcacttt
2041 ctcattgagg gtgatctaaa tccggacaac tcggatgtcg acaaactgtt catccagtta
2101 gtacaaacct ataatcagtt gtttgaagag aaccctataa atgcaagtgg cgtggatgcg
2161 aaggctattc ttagcgcccg cctctctaaa tcccgacggc tagaaaacct gatcgcacaa
2221 ttacccggag agaagaaaaa tgggttgttc ggtaacctta tagcgctctc actaggcctg
2281 acaccaaatt ttaagtcgaa cttcgactta gctgaagatg ccaaattgca gcttagtaag
2341 gacacgtacg atgacgatct cgacaatcta ctggcacaaa ttggagatca gtatgcggac
2401 ttatttttgg ctgccaaaaa ccttagcgat gcaatcctcc tatctgacat actgagagtt
2461 aatactgaga ttaccaaggc gccgttatcc gcttcaatga tcaaaaggta cgatgaacat
2521 caccaagact tgacacttct caaggcccta gtccgtcagc aactgcctga gaaatataag
2581 gaaatattct ttgatcagtc gaaaaacggg tacgcaggtt atattgacgg cggagcgagt
2641 caagaggaat tctacaagtt tatcaaaccc atattagaga agatggatgg gacggaagag
2701 ttgcttgtaa aactcaatcg cgaagatcta ctgcgaaagc agcggacttt cgacaacggt
2761 agcattccac atcaaatcca cttaggcgaa ttgcatgcta tacttagaag gcaggaggat
2821 ttttatccgt tcctcaaaga caatcgtgaa aagattgaga aaatcctaac ctttcgcata
2881 ccttactatg tgggacccct ggcccgaggg aactctcggt tcgcatggat gacaagaaag
2941 tccgaagaaa cgattactcc atggaatttt gaggaagttg tcgataaagg tgcgtcagct
3001 caatcgttca tcgagaggat gaccaacttt gacaagaatt taccgaacga aaaagtattg
3061 cctaagcaca gtttacttta cgagtatttc acagtgtaca atgaactcac gaaagttaag
3121 tatgtcactg agggcatgcg taaacccgcc tttctaagcg gagaacagaa gaaagcaata
3181 gtagatctgt tattcaagac caaccgcaaa gtgacagtta agcaattgaa agaggactac
3241 tttaagaaaa ttgaatgctt cgattctgtc gagatctccg gggtagaaga tcgatttaat
3301 gcgtcacttg gtacgtatca tgacctccta aagataatta aagataagga cttcctggat
3361 aacgaagaga atgaagatat cttagaagat atagtgttga ctcttaccct ctttgaagat
3421 cgggaaatga ttgaggaaag actaaaaaca tacgctcacc tgttcgacga taaggttatg
3481 aaacagttaa agaggcgtcg ctatacgggc tggggacgat tgtcgcggaa acttatcaac
3541 gggataagag acaagcaaag tggtaaaact attctcgatt ttctaaagag cgacggcttc
3601 gccaatagga actttatgca gctgatccat gatgactctt taaccttcaa agaggatata
3661 caaaaggcac aggtttccgg acaaggggac tcattgcacg aacatattgc gaatcttgct
3721 ggttcgccag ccatcaaaaa gggcatactc cagacagtca aagtagtgga tgagctagtt
3781 aaggtcatgg gacgtcacaa accggaaaac attgtaatcg agatggcacg cgaaaatcaa
3841 acgactcaga aggggcaaaa aaacagtcga gagcggatga agagaataga agagggtatt
3901 aaagaactgg gcagccagat cttaaaggag catcctgtgg aaaataccca attgcagaac
3961 gagaaacttt acctctatta cctacaaaat ggaagggaca tgtatgttga tcaggaactg
4021 gacataaacc gtttatctga ttacgacgtc gatcacattg taccccaatc ctttttgaag
4081 gacgattcaa tcgacaataa agtgcttaca cgctcggata agaaccgagg gaaaagtgac
4141 aatgttccaa gcgaggaagt cgtaaagaaa atgaagaact attggcggca gctcctaaat
4201 gcgaaactga taacgcaaag aaagttcgat aacttaacta aagctgagag gggtggcttg
4261 tctgaacttg acaaggccgg atttattaaa cgtcagctcg tggaaacccg ccaaatcaca
4321 aagcatgttg cacagatact agattcccga atgaatacga aatacgacga gaacgataag
4381 ctgattcggg aagtcaaagt aatcacttta aagtcaaaat tggtgtcgga cttcagaaag
4441 gattttcaat tctataaagt tagggagata aataactacc accatgcgca cgacgcttat
4501 cttaatgccg tcgtagggac cgcactcatt aagaaatacc cgaagctaga aagtgagttt
4561 gtgtatggtg attacaaagt ttatgacgtc cgtaagatga tcgcgaaaag cgaacaggag
4621 ataggcaagg ctacagccaa atacttcttt tattctaaca ttatgaattt ctttaagacg
4681 gaaatcactc tggcaaacgg agagatacgc aaacgacctt taattgaaac caatggggag
4741 acaggtgaaa tcgtatggga taagggccgg gacttcgcga cggtgagaaa agttttgtcc
4801 atgccccaag tcaacatagt aaagaaaact gaggtgcaga ccggagggtt ttcaaaggaa
4861 tcgattcttc caaaaaggaa tagtgataag ctcatcgctc gtaaaaagga ctgggacccg
4921 aaaaagtacg gtggcttcga tagccctaca gttgcctatt ctgtcctagt agtggcaaaa
4981 gttgagaagg gaaaatccaa gaaactgaag tcagtcaaag aattattggg gataacgatt
5041 atggagcgct cgtcttttga aaagaacccc atcgacttcc ttgaggcgaa aggttacaag
5101 gaagtaaaaa aggatctcat aattaaacta ccaaagtata gtctgtttga gttagaaaat
5161 ggccgaaaac ggatgttggc tagcgccgga gagcttcaaa aggggaacga actcgcacta
5221 ccgtctaaat acgtgaattt cctgtattta gcgtcccatt acgagaagtt gaaaggttca
5281 cctgaagata acgaacagaa gcaacttttt gttgagcagc acaaacatta tctcgacgaa
5341 atcatagagc aaatttcgga attcagtaag agagtcatcc tagctgatgc caatctggac
5401 aaagtattaa gcgcatacaa caagcacagg gataaaccca tacgtgagca ggcggaaaat
5461 attatccatt tgtttactct taccaacctc ggcgctccag ccgcattcaa gtattttgac
5521 acaacgatag atcgcaaacg atacacttct accaaggagg tgctagacgc gacactgatt
5581 caccaatcca tcacgggatt atatgaaact cggatagatt tgtcacagct tgggggtgac
5641 tctggtggtt ctactaatct gtcagatatt attgaaaagg agaccggtaa gcaactggtt
5701 atccaggaat ccatcctcat gctcccagag gaggtggaag aagtcattgg gaacaagccg
5761 gaaagcgata tactcgtgca caccgcctac gacgagagca ccgacgagaa tgtcatgctt
5821 ctgactagcg acgcccctga atacaagcct tgggctctgg tcatacagga tagcaacggt
5881 gagaacaaga ttaagatgct ctctggtggt tctcccaaga agaagaggaa agtctaaccg
5941 gtcatcatca ccatcaccat tgagtttaaa cccgctgatc agcctcgact gtgccttcta
6001 gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca
6061 ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc
6121 attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagacaata
6181 gcaggcatgc tggggatgcg gtgggctcta tggcttctga ggcggaaaga accagctggg
6241 gctcgatacc gtcgacctct agctagagct tggcgtaatc atggtcatag ctgtttcctg
6301 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta
6361 aagcctaggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg
6421 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga
6481 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
6541 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
6601 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
6661 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
6721 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
6781 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
6841 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
6901 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
6961 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
7021 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
7081 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
7141 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
7201 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
7261 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
7321 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
7381 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
7441 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
7501 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
7561 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
7621 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
7681 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
7741 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
7801 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
7861 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
7921 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
7981 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
8041 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
8101 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
8161 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
8221 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
8281 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
8341 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
8401 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt cgacggatcg ggagatcgat
8461 ctcccgatcc cctagggtcg actctcagta caatctgctc tgatgccgca tagttaagcc
8521 agtatctgct cc
//
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pCMV-BE3质粒载体应用举例
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