pCAGGS-Flag-hsDicer

LOCUS       pCAGGS-Flag-hsDicer               10794 bp ds-DNA     circular SYN 15-JUN-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pCAGGS-Flag-hsDicer
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10794)
  AUTHORS   Gurtan AM, Lu V, Bhutkar A, Sharp PA
  TITLE     In vivo structure-function analysis of human Dicer reveals 
            directional processing of precursor miRNAs.
  JOURNAL   RNA. 2012 Jun;18(6):1116-22. Epub 2012 Apr 30.
  PUBMED    22546613
REFERENCE   2  (bases 1 to 10794)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jun 15, 2022 from SnapGene Server 1.1.58
            http://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..10794
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     gap             30..44
                     /estimated_length=15
     intron          272..1288
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1212..1234
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1296..1315
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             1362..1385
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(7164..7183)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    7229..7284
                     /label=-beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(7283..7302)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(7645..7661)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7645..7661)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7658..7680)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7669..7685
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(7693..7723)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7738..7759
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        7818..8013
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      7864..7999
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7926..7945
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     polyA_signal    8019..8153
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8056..8075)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8110..8129
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8221..8238)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8392..8980)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8472..8491)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(9151..10011)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9774..9793
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10012..10116)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        10147..10526
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gggggggggg gggggggggg gggggggggn nnnnnnnnnn nnnnsssssv dddttgktdt
       61 tkkttddttk kddadktntk kkgggggggg gggggggggg gggggggggg gggcgcgnnc
      121 gcsrggsggg gsggggsggg gcgnggggsg gggsggggcg aggcggagag gtgcggcggc
      181 agccaatcag agcggcgcgc tccgaaagtt tccttttatg gcgaggcggc ggcggcggcg
      241 gccctataaa aagcgaagcg cgcggcgggc gggagtcgct gcgcgctgcc ttcgccccgt
      301 gccccgctcc gccgccgcct cgcgccgccc gccccggctc tgactgaccg cgttactccc
      361 acaggtgagc gggcgggacg gcccttctcc tccgggctgt aattagcgct tggtttaatg
      421 acggcttgtt tcttttctgt ggctgcgtga aagccttgag gggctccggg agggcccttt
      481 gtgcgggggg agcggctcgg ggggtgcgtg cgtgtgtgtg tgcgtgggga gcgccgcgtg
      541 cggctccgcg ctgcccggcg gctgtgagcg ctgcgggcgc ggcgcggggc tttgtgcgct
      601 ccgcagtgtg cgcgagggga gcgcggccgg gggcggtgcc ccgcggtgcg gggggggctg
      661 cgaggggaac aaaggctgcg tgcggggtgt gtgcgtgggg gggtgagcag ggggtgtggg
      721 cgcgtcggtc gggctgcaac cccccctgca cccccctccc cgagttgctg agcacggccc
      781 ggcttcgggt gcggggctcc gtacggggcg tggcgcgggg ctcgccgtgc cgggcggggg
      841 gtggcggcag gtgggggtgc cgggcggggc ggggccgcct cgggccgggg rgggctcggg
      901 ggaggggcgc ggcggccccc ggagcgccgg cggctgtcga ggcgcggcga gccgcagcca
      961 ttgcctttta tggtaatcgt gcgagagggc gcagggactt cctttgtccc aaatctgtgc
     1021 ggagccgaaa tctgggaggc gccgccgcac cccctctagc gggcgcgggg cgaagcggtg
     1081 cggcgccggc aggaaggaaa tgggcgggga gggccttcgt gcgtcgccgc gccgccgtcc
     1141 ccttctccct ctccagcctc ggggctgtcc gcggggggac ggctgccttc gggggggacg
     1201 gggcagggcg gggttcggct tctggcgtgt gaccggcggc tctagagcct ctgctaacca
     1261 tgttcatgcc ttcttctttt tcctacagct cctgggcaac gtgctggtta ttgtgctgtc
     1321 tcatcatttt ggcaaagaat tctcgagatt gatctaccat ggactacaaa gacgatgacg
     1381 acaagcttaa aagccctgct ttgcaacccc tcagcatggc aggcctgcag ctcatgaccc
     1441 ctgcttcctc accaatgggt cctttctttg gactgccatg gcaacaagaa gcaattcatg
     1501 ataacattta tacgccaaga aaatatcagg ttgaactgct tgaagcagct ctggatcata
     1561 ataccatcgt ctgtttaaac actggctcag ggaagacatt tattgcagta ctactcacta
     1621 aagagctgtc ctatcagatc aggggagact tcagcagaaa tggaaaaagg acggtgttct
     1681 tggtcaactc tgcaaaccag gttgctcaac aagtgtcagc tgtcagaact cattcagatc
     1741 tcaaggttgg ggaatactca aacctagaag taaatgcatc ttggacaaaa gagagatgga
     1801 accaagagtt tactaagcac caggttctca ttatgacttg ctatgtcgcc ttgaatgttt
     1861 tgaaaaatgg ttacttatca ctgtcagaca ttaacctttt ggtgtttgat gagtgtcatc
     1921 ttgcaatcct agaccacccc tatcgagaaa ttatgaagct ctgtgaaaat tgtccatcat
     1981 gtcctcgcat tttgggacta actgcttcca ttttaaatgg gaaatgtgat ccagaggaat
     2041 tggaagaaaa gattcagaaa ctagagaaaa ttcttaagag taatgctgaa actgcaactg
     2101 acctggtggt cttagacagg tatacttctc agccatgtga gattgtggtg gattgtggac
     2161 catttactga cagaagtggg ctttatgaaa gactgctgat ggaattagaa gaagcactta
     2221 attttatcaa tgattgtaat atatctgtac attcaaaaga aagagattct actttaattt
     2281 cgaaacagat actatcagac tgtcgtgccg tattggtagt tctgggaccc tggtgtgcag
     2341 ataaagtagc tggaatgatg gtaagagaac tacagaaata catcaaacat gagcaagagg
     2401 agctgcacag gaaattttta ttgtttacag acactttcct aaggaaaata catgcactat
     2461 gtgaagagca cttctcacct gcctcacttg acctgaaatt tgtaactcct aaagtaatca
     2521 aactgctcga aatcttacgc aaatataaac catatgagcg acagcagttt gaaagcgttg
     2581 agtggtataa taatagaaat caggataatt atgtgtcatg gagtgattct gaggatgatg
     2641 atgaggatga agaaattgaa gaaaaagaga agccagagac aaattttcct tctcctttta
     2701 ccaacatttt gtgcggaatt atttttgtgg aaagaagata cacagcagtt gtcttaaaca
     2761 gattgataaa ggaagctggc aaacaagatc cagagctggc ttatatcagt agcaatttca
     2821 taactggaca tggcattggg aagaatcagc ctcgcaacaa acagatggaa gcagaattca
     2881 gaaaacagga agaggtactt aggaaatttc gagcacatga gaccaacctg cttattgcaa
     2941 caagtattgt agaagagggt gttgatatac caaaatgcaa cttggtggtt cgttttgatt
     3001 tgcccacaga atatcgatcc tatgttcaat ctaaaggaag agcaagggca cccatctcta
     3061 attatataat gttagcggat acagacaaaa taaaaagttt tgaagaagac cttaaaacct
     3121 acaaagctat tgaaaagatc ttgagaaaca agtgttccaa gtcggttgat actggtgaga
     3181 ctgacattga tcctgtcatg gatgatgatg acgttttccc accatatgtg ttgaggcctg
     3241 acgatggtgg tccacgagtc acaatcaaca cggccattgg acacatcaat agatactgtg
     3301 ctagattacc aagtgatccg tttactcatc tagctcctaa atgcagaacc cgagagttgc
     3361 ctgatggtac attttattca actctttatc tgccaattaa ctcacctctt cgagcctcca
     3421 ttgttggtcc accaatgagc tgtgtacgat tggctgaaag agttgtagct ctcatttgct
     3481 gtgagaaact gcacaaaatt ggcgaactgg atgaccattt gatgccagtt gggaaagaga
     3541 ctgttaaata tgaagaggag cttgatttgc atgatgaaga agagaccagt gttccaggaa
     3601 gaccaggttc cacgaaacga aggcagtgct acccaaaagc aattccagag tgtttgaggg
     3661 atagttatcc cagacctgat cagccctgtt acctgtatgt gataggaatg gttttaacta
     3721 cacctttacc tgatgaactc aactttagaa ggcggaagct ctatcctcct gaagatacca
     3781 caagatgctt tggaatactg acggccaaac ccatacctca gattccacac tttcctgtgt
     3841 acacacgctc tggagaggtt accatatcca ttgagttgaa gaagtctggt ttcatgttgt
     3901 ctctacaaat gcttgagttg attacaagac ttcaccagta tatattctca catattcttc
     3961 ggcttgaaaa acctgcacta gaatttaaac ctacagacgc tgattcagca tactgtgttc
     4021 tacctcttaa tgttgttaat gactccagca ctttggatat tgactttaaa ttcatggaag
     4081 atattgagaa gtctgaagct cgcataggca ttcccagtac aaagtataca aaagaaacac
     4141 cctttgtttt taaattagaa gattaccaag atgccgttat cattccaaga tatcgcaatt
     4201 ttgatcagcc tcatcgattt tatgtagctg atgtgtacac tgatcttacc ccactcagta
     4261 aatttccttc ccctgagtat gaaacttttg cagaatatta taaaacaaag tacaaccttg
     4321 acctaaccaa tctcaaccag ccactgctgg atgtggacca cacatcttca agacttaatc
     4381 ttttgacacc tcgacatttg aatcagaagg ggaaagcgct tcctttaagc agtgctgaga
     4441 agaggaaagc caaatgggaa agtctgcaga ataaacagat actggttcca gaactctgtg
     4501 ctatacatcc aattccagca tcactgtgga gaaaagctgt ttgtctcccc agcatacttt
     4561 atcgccttca ctgccttttg actgcagagg agctaagagc ccagactgcc agcgatgctg
     4621 gcgtgggagt cagatcactt cctgcggatt ttagataccc taacttagac ttcgggtgga
     4681 aaaaatctat tgacagcaaa tctttcatct caatttctaa ctcctcttca gctgaaaatg
     4741 ataattactg taagcacagc acaattgtcc ctgaaaatgc tgcacatcaa ggtgctaata
     4801 gaacctcctc tctagaaaat catgaccaaa tgtctgtgaa ctgcagaacg ttgctcagcg
     4861 agtcccctgg taagctccac gttgaagttt cagcagatct tacagcaatt aatggtcttt
     4921 cttacaatca aaatctcgcc aatggcagtt atgatttagc taacagagac ttttgccaag
     4981 gaaatcagct aaattactac aagcaggaaa tacccgtgca accaactacc tcatattcca
     5041 ttcagaattt atacagttac gagaaccagc cccagcccag cgatgaatgt actctcctga
     5101 gtaataaata ccttgatgga aatgctaaca aatctacctc agatggaagt cctgtgatgg
     5161 ccgtaatgcc tggtacgaca gacactattc aagtgctcaa gggcaggatg gattctgagc
     5221 agagcccttc tattgggtac tcctcaagga ctcttggccc caatcctgga cttattcttc
     5281 aggctttgac tctgtcaaac gctagtgatg gatttaacct ggagcggctt gaaatgcttg
     5341 gcgactcctt tttaaagcat gccatcacca catatctatt ttgcacttac cctgatgcgc
     5401 atgagggccg cctttcatat atgagaagca aaaaggtcag caactgtaat ctgtatcgcc
     5461 ttggaaaaaa gaagggacta cccagccgca tggtggtgtc aatatttgat ccccctgtga
     5521 attggcttcc tcctggttat gtagtaaatc aagacaaaag caacacagat aaatgggaaa
     5581 aagatgaaat gacaaaagac tgcatgctgg cgaatggcaa actggatgag gattacgagg
     5641 aggaggatga ggaggaggag agcctgatgt ggagggctcc gaaggaagag gctgactatg
     5701 aagatgattt cctggagtat gatcaggaac atatcagatt tatagataat atgttaatgg
     5761 ggtcaggagc ttttgtaaag aaaatctctc tttctccttt ttcaaccact gattctgcat
     5821 atgaatggaa aatgcccaaa aaatcctcct taggtagtat gccattttca tcagattttg
     5881 aggattttga ctacagctct tgggatgcaa tgtgctatct ggatcctagc aaagctgttg
     5941 aagaagatga ctttgtggtg gggttctgga atccatcaga agaaaactgt ggtgttgaca
     6001 cgggaaagca gtccatttct tacgacttgc acactgagca gtgtattgct gacaaaagca
     6061 tagcggactg tgtggaagcc ctgctgggct gctatttaac cagctgtggg gagagggctg
     6121 ctcagctttt cctctgttca ctggggctga aggtgctccc ggtaattaaa aggactgatc
     6181 gggaaaaggc cctgtgccct actcgggaga atttcaacag ccaacaaaag aacctttcag
     6241 tgagctgtgc tgctgcttct gtggccagtt cacgctcttc tgtattgaaa gactcggaat
     6301 atggttgttt gaagattcca ccaagatgta tgtttgatca tccagatgca gataaaacac
     6361 tgaatcacct tatatcgggg tttgaaaatt ttgaaaagaa aatcaactac agattcaaga
     6421 ataaggctta ccttctccag gcttttacac atgcctccta ccactacaat actatcactg
     6481 attgttacca gcgcttagaa ttcctgggag atgcgatttt ggactacctc ataaccaagc
     6541 acctttatga agacccgcgg cagcactccc cgggggtcct gacagacctg cggtctgccc
     6601 tggtcaacaa caccatcttt gcatcgctgg ctgtaaagta cgactaccac aagtacttca
     6661 aagctgtctc tcctgagctc ttccatgtca ttgatgactt tgtgcagttt cagcttgaga
     6721 agaatgaaat gcaaggaatg gattctgagc ttaggagatc tgaggaggat gaagagaaag
     6781 aagaggatat tgaagttcca aaggccatgg gggatatttt tgagtcgctt gctggtgcca
     6841 tttacatgga tagtgggatg tcactggaga cagtctggca ggtgtactat cccatgatgc
     6901 ggccactaat agaaaagttt tctgcaaatg taccccgttc ccctgtgcga gaattgcttg
     6961 aaatggaacc agaaactgcc aaatttagcc cggctgagag aacttacgac gggaaggtca
     7021 gagtcactgt ggaagtagta ggaaagggga aatttaaagg tgttggtcga agttacagga
     7081 ttgccaaatc tgcagcagca agaagagccc tccgaagcct caaagctaat caacctcagg
     7141 ttcccaatag ctgaggtacc agatcttttt ccctctgcca aaaattatgg ggacatcatg
     7201 aagccccttg agcatctgac ttctggctaa taaaggaaat ttattttcat tgcaatagtg
     7261 tgttggaatt ttttgtgtct ctcactcgga aggacatatg ggagggcaaa tcatttaaaa
     7321 catcagaatg agtatttggt ttagagtttg gcaacatatg cccatatgct ggctgccatg
     7381 aacaaaggtt ggctataaag aggtcatcag tatatgaaac agccccctgc tgtccattcc
     7441 ttattccata gaaaagcctt gacttgaggt tagatttttt ttatattttg ttttgtgtta
     7501 tttttttctt taacatccct aaaattttcc ttacatgttt tactagccag atttttcctc
     7561 ctctcctgac tactcccagt catagctgtc cctcttctct tatggagatc cctcgacctg
     7621 cagcccaagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct
     7681 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg
     7741 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
     7801 gtcgtgccag cggatccgca tctcaattag tcagcaacca tagtcccgcc cctaactccg
     7861 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt
     7921 ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga
     7981 ggaggctttt ttggaggcct aggcttttgc aaaaagctaa cttgtttatt gcagcttata
     8041 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
     8101 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg atccgctgca
     8161 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc
     8221 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
     8281 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
     8341 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     8401 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     8461 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     8521 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     8581 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     8641 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     8701 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     8761 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     8821 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     8881 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     8941 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     9001 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     9061 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
     9121 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
     9181 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
     9241 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
     9301 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
     9361 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
     9421 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
     9481 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
     9541 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
     9601 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
     9661 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
     9721 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
     9781 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
     9841 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
     9901 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
     9961 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
    10021 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
    10081 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
    10141 tgggtcgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc
    10201 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
    10261 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
    10321 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
    10381 tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc
    10441 ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct
    10501 acgtattagt catcgctatt accatggtcg aggtgagccc cacgttctgc ttcactctcc
    10561 ccatctcccc cccctcccca cccccaattt tgtatttatt tattttttaa ttattttgtg
    10621 cagcgatggg ggcggggggg gggggggggn sgcgssccrg gggggggggg gggggggggg
    10681 gggggggggg gggggggggg gggggggggg gggggggsgn svnvgggggg gggggsgngg
    10741 grgggkgbgk kgnngggggg gggggggggg gggggggggg gggggggggg gggg
//