| 质粒名称: | 2XMyc-LRRK2-D1994A |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 25368 |
| 存储实验室: | Mark Cookson |
|
载体骨架基本信息 |
|
| 载体名称: | pCMV-Tag 3B |
| 载体出品公司: | Cookson Lab |
| 空载体大小: | 4300bp |
| 完整质粒大小: | 11985bp |
| 载体类型: | 哺乳细胞表达 |
| 载体抗性: | Kanamycin, 50 μg/mL |
| 生长温度: | 37 ℃ |
| 宿主菌: | Stbl3 |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | LRRK2 |
| 物种来源: | H. sapiens (human) |
| 插入大小: | 7581bp |
| 基因突变: |
Aspartate 1994 mutated to Alanine
Entrez GeneLRRK2 (a.k.a. AURA17, DARDARIN, PARK8, RIPK7, ROCO2)
|
| 标签/融合蛋白: | 2XMyc (N terminal on insert) |
| 克隆方法: | 限制性内切酶 |
| 5’测序引物 | CMV-F |
| 3’测序引物 | SV40pA-R |
|
文献引用方法 |
|
| 材料和方法部分: | 2XMyc-LRRK2-D1994A was a gift from Mark Cookson (Addgene plasmid # 25368 ; http://n2t.net/addgene:25368 ; RRID:Addgene_25368) |
| 参考文献部分: | The Parkinson Disease-associated Leucine-rich Repeat Kinase 2 (LRRK2) Is a Dimer That Undergoes Intramolecular Autophosphorylation. . J Biol Chem. 2008 Jun 13;283(24):16906-14. 10.1074/jbc.M708718200 PubMed 18397888 |
买家导航
2XMyc-LRRK2-D1994A载体质粒基本信息
2XMyc-LRRK2-D1994A质粒图谱载体图谱和2XMyc-LRRK2-D1994A载体序列质粒序列多克隆位点信息
2XMyc-LRRK2-D1994A质粒载体简介
2XMyc-LRRK2-D1994A质粒序列载体序列
LOCUS 2XMyc-LRRK2-D1994A 11985 bp DNA circular SYN 14-APR-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 11985)
TITLE The Parkinson Disease-associated Leucine-rich Repeat Kinase 2
(LRRK2) Is a Dimer That Undergoes Intramolecular Autophosphorylation
JOURNAL J Biol Chem. 2008 Jun 13;283(24):16906-14.
PUBMED 18397888
REFERENCE 2 (bases 1 to 11985)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Apr 14, 2025 from SnapGene Server 8.0.1
https://www.biofeng.com
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "J Biol
Chem."; date: "2008-06-13"; volume: "283(24)"; pages: "16906-14"
COMMENT Sequence Label: 2XMyc-LRRK2-D1994A
FEATURES Location/Qualifiers
source 1..11985
/mol_type="other DNA"
/organism="synthetic DNA construct"
enhancer 81..384
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 385..588
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 539..559
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
promoter 634..652
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
regulatory 684..693
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
CDS 693..722
/codon_start=1
/product="Myc (human c-Myc proto-oncogene) epitope tag"
/label=Myc
/translation="EQKLISEEDL"
CDS 738..767
/codon_start=1
/product="Myc (human c-Myc proto-oncogene) epitope tag"
/label=Myc
/translation="EQKLISEEDL"
protein_bind 786..810
/gene="mutant version of attB"
/label=attB1
/bound_moiety="BP Clonase(TM)"
/note="recombination site for the Gateway(R) BP reaction"
protein_bind complement(8427..8451)
/gene="mutant version of attB"
/label=attB2
/bound_moiety="BP Clonase(TM)"
/note="recombination site for the Gateway(R) BP reaction"
primer_bind complement(8500..8519)
/label=T7
/note="T7 promoter, forward primer"
promoter complement(8501..8519)
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
polyA_signal 8793..8914
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(8830..8849)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 8884..8903
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
rep_origin complement(8921..9376)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(9058..9079)
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind 9270..9289
/label=F1ori-R
/note="F1 origin, reverse primer"
promoter 9403..9507
/gene="bla"
/label=AmpR promoter
promoter 9509..9866
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
enhancer 9509..9700
/label=SV40 enhancer
/note="enhancer for the SV40 early promoter (Herr, 1993)"
primer_bind complement(9532..9552)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
rep_origin 9717..9852
/label=SV40 ori
/note="SV40 origin of replication"
CDS 9901..10695
/codon_start=1
/product="aminoglycoside phosphotransferase"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
CDS 9901..10695
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(9955..9974)
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
primer_bind 10565..10584
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
primer_bind complement(10883..10902)
/label=TK-pA-R
/note="Thymidine kinase polyA, reverse primer"
polyA_signal 10927..10974
/label=HSV TK poly(A) signal
/note="herpes simplex virus thymidine kinase
polyadenylation signal (Cole and Stacy, 1985)"
rep_origin 11303..11891
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 11792..11811
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
ORIGIN
1 accgtattac cgccatgcat tagttattaa tagtaatcaa ttacggggtc attagttcat
61 agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg
121 cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata
181 gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta
241 catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc
301 gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
361 gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga
421 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg
481 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg
541 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctgg tttagtgaac
601 cgtcagatcc gctagcgatt acgccaagct cgaaattaac cctcactaaa gggaacaaaa
661 gctggagctc caccgcggtg gcggccgcca tggagcagaa actcatctct gaagaggatc
721 tgagcccggg cggatccgag cagaaactca tctctgaaga ggatctgctg caggaattcg
781 atatcacaag tttgtacaaa aaagcaggct ccgaattcgc ccttatggct agtggcagct
841 gtcaggggtg cgaagaggac gaggaaactc tgaagaagtt gatagtcagg ctgaacaatg
901 tccaggaagg aaaacagata gaaacgctgg tccaaatcct ggaggatctg ctggtgttca
961 cgtactccga gcacgcctcc aagttatttc aaggcaaaaa tatccatgtg cctctgttga
1021 tcgtcttgga ctcctatatg agagtcgcga gtgtgcagca ggtgggttgg tcacttctgt
1081 gcaaattaat agaagtctgt ccaggtacaa tgcaaagctt aatgggaccc caggatgttg
1141 gaaatgattg ggaagtcctt ggtgttcacc aattgattct taaaatgcta acagttcata
1201 atgccagtgt aaacttgtca gtgattggac tgaagacctt agatctcctc ctaacttcag
1261 gtaaaatcac cttgctgata ttggatgaag aaagtgatat tttcatgtta atttttgatg
1321 ccatgcactc atttccagcc aatgatgaag tccagaaact tggatgcaaa gctttacatg
1381 tgctgtttga gagagtctca gaggagcaac tgactgaatt tgttgagaac aaagattata
1441 tgatattgtt aagtgcgtta acaaatttta aagatgaaga ggaaattgtg cttcatgtgc
1501 tgcattgttt acattcccta gcgattcctt gcaataatgt ggaagtcctc atgagtggca
1561 atgtcaggtg ttataatatt gtggtggaag ctatgaaagc attccctatg agtgaaagaa
1621 ttcaagaagt gagttgctgt ttgctccata ggcttacatt aggtaatttt ttcaatatcc
1681 tggtattaaa cgaagtccat gagtttgtgg tgaaagctgt gcagcagtac ccagagaatg
1741 cagcattgca gatctcagcg ctcagctgtt tggccctcct cactgagact attttcttaa
1801 atcaagattt agaggaaaag aatgagaatc aagagaatga tgatgagggg gaagaagata
1861 aattgttttg gctggaagcc tgttacaaag cattaacgtg gcatagaaag aacaagcacg
1921 tgcaggaggc cgcatgctgg gcactaaata atctccttat gtaccaaaac agtttacatg
1981 agaagattgg agatgaagat ggccatttcc cagctcatag ggaagtgatg ctctccatgc
2041 tgatgcattc ttcatcaaag gaagttttcc aggcatctgc gaatgcattg tcaactctct
2101 tagaacaaaa tgttaatttc agaaaaatac tgttatcaaa aggaatacac ctgaatgttt
2161 tggagttaat gcagaagcat atacattctc ccgaagtggc tgaaagtggc tgtaaaatgc
2221 taaatcatct ttttgaagga agcaacactt ccctggatat aatggcagca gtggtcccca
2281 aaatactaac agttatgaaa cgtcatgaga catcattacc agtgcagctg gaggcgcttc
2341 gagctatttt acattttata gtgcctggca tgccagaaga atccagggag gatacagaat
2401 ttcatcataa gctaaatatg gttaaaaaac agtgtttcaa gaatgatatt cacaaactgg
2461 tcctagcagc tttgaacagg ttcattggaa atcctgggat tcagaaatgt ggattaaaag
2521 taatttcttc tattgtacat tttcctgatg cattagagat gttatccctg gaaggtgcta
2581 tggattcagt gcttcacaca ctgcagatgt atccagatga ccaagaaatt cagtgtctgg
2641 gtttaagtct tataggatac ttgattacaa agaagaatgt gttcatagga actggacatc
2701 tgctggcaaa aattctggtt tccagcttat accgatttaa ggatgttgct gaaatacaga
2761 ctaaaggatt tcagacaatc ttagcaatcc tcaaattgtc agcatctttt tctaagctgc
2821 tggtgcatca ttcatttgac ttagtaatat tccatcaaat gtcttccaat atcatggaac
2881 aaaaggatca acagtttcta aacctctgtt gcaagtgttt tgcaaaagta gctatggatg
2941 attacttaaa aaatgtgatg ctagagagag cgtgtgatca gaataacagc atcatggttg
3001 aatgcttgct tctattggga gcagatgcca atcaagcaaa ggagggatct tctttaattt
3061 gtcaggtatg tgagaaagag agcagtccca aattggtgga actcttactg aatagtggat
3121 ctcgtgaaca agatgtacga aaagcgttga cgataagcat tgggaaaggt gacagccaga
3181 tcatcagctt gctcttaagg aggctggccc tggatgttgc caacaatagc atttgccttg
3241 gaggattttg tataggaaaa gttgaacctt cttggcttgg tcctttattt ccagataaga
3301 cttctaattt aaggaaacaa acaaatatag catctacact agcaagaatg gtgatcagat
3361 atcagatgaa aagtgctgtg gaagaaggaa cagcctcagg cagcgatgga aatttttctg
3421 aagatgtgct gtctaaattt gatgaatgga cctttattcc tgactcttct atggacagtg
3481 tgtttgctca aagtgatgac ctggatagtg aaggaagtga aggctcattt cttgtgaaaa
3541 agaaatctaa ttcaattagt gtaggagaat tttaccgaga tgccgtatta cagcgttgct
3601 caccaaattt gcaaagacat tccaattcct tggggcccat ttttgatcat gaagatttac
3661 tgaagcgaaa aagaaaaata ttatcttcag atgattcact caggtcatca aaacttcaat
3721 cccatatgag gcattcagac agcatttctt ctctggcttc tgagagagaa tatattacat
3781 cactagacct ttcagcaaat gaactaagag atattgatgc cctaagccag aaatgctgta
3841 taagtgttca tttggagcat cttgaaaagc tggagcttca ccagaatgca ctcacgagct
3901 ttccacaaca gctatgtgaa actctgaaga gtttgacaca tttggacttg cacagtaata
3961 aatttacatc atttccttct tatttgttga aaatgagttg tattgctaat cttgatgtct
4021 ctcgaaatga cattggaccc tcagtggttt tagatcctac agtgaaatgt ccaactctga
4081 aacagtttaa cctgtcatat aaccagctgt cttttgtacc tgagaacctc actgatgtgg
4141 tagagaaact ggagcagctc attttagaag gaaataaaat atcagggata tgctccccct
4201 tgagactgaa ggaactgaag attttaaacc ttagtaagaa ccacatttca tccctatcag
4261 agaactttct tgaggcttgt cctaaagtgg agagtttcag tgccagaatg aattttcttg
4321 ctgctatgcc tttcttgcct ccttctatga caatcctaaa attatctcag aacaaatttt
4381 cctgtattcc agaagcaatt ttaaatcttc cacacttgcg gtctttagat atgagcagca
4441 atgatattca gtacctacca ggtcccgcac actggaaatc tttgaactta agggaactct
4501 tatttagcca taatcagatc agcatcttgg acttgagtga aaaagcatat ttatggtcta
4561 gagtagagaa actgcatctt tctcacaata aactgaaaga gattcctcct gagattggct
4621 gtcttgaaaa tctgacatct ctggatgtca gttacaactt ggaactaaga tcctttccca
4681 atgaaatggg gaaattaagc aaaatatggg atcttccttt ggatgaactg catcttaact
4741 ttgattttaa acatatagga tgtaaagcca aagacatcat aaggtttctt caacagcgat
4801 taaaaaaggc tgtgccttat aaccgaatga aacttatgat tgtgggaaat actgggagtg
4861 gtaaaaccac cttattgcag caattaatga aaaccaagaa atcagatctt ggaatgcaaa
4921 gtgccacagt tggcatagat gtgaaagact ggcctatcca aataagagac aaaagaaaga
4981 gagatctcgt cctaaatgtg tgggattttg caggtcgtga ggaattctat agtactcatc
5041 cccattttat gacgcagcga gcattgtacc ttgctgtcta tgacctcagc aagggacagg
5101 ctgaagttga tgccatgaag ccttggctct tcaatataaa ggctcgcgct tcttcttccc
5161 ctgtgattct cgttggcaca catttggatg tttctgatga gaagcaacgc aaagcctgca
5221 tgagtaaaat caccaaggaa ctcctgaata agcgagggtt ccctgccata cgagattacc
5281 actttgtgaa tgccaccgag gaatctgatg ctttggcaaa acttcggaaa accatcataa
5341 acgagagcct taatttcaag atccgagatc agcttgttgt tggacagctg attccagact
5401 gctatgtaga acttgaaaaa atcattttat cggagcgtaa aaatgtgcca attgaatttc
5461 ccgtaattga ccggaaacga ttattacaac tagtgagaga aaatcagctg cagttagatg
5521 aaaatgagct tcctcacgca gttcactttc taaatgaatc aggagtcctt cttcattttc
5581 aagacccagc actgcagtta agtgacttgt actttgtgga acccaagtgg ctttgtaaaa
5641 tcatggcaca gattttgaca gtgaaagtgg aaggttgtcc aaaacaccct aagggaatta
5701 tttcgcgtag agatgtggaa aaatttcttt caaaaaaaag gaaatttcca aagaactaca
5761 tgtcacagta ttttaagctc ctagaaaaat tccagattgc tttgccaata ggagaagaat
5821 atttgctggt tccaagcagt ttgtctgacc acaggcctgt gatagagctt ccccattgtg
5881 agaactctga aattatcatc cgactatatg aaatgcctta ttttccaatg ggattttggt
5941 caagattaat caatcgatta cttgagattt caccttacat gctttcaggg agagaacgag
6001 cacttcgccc aaacagaatg tattggcgac aaggcattta cttaaattgg tctcctgaag
6061 cttattgtct ggtaggatct gaagtcttag acaatcatcc agagagtttc ttaaaaatta
6121 cagttccttc ttgtagaaaa ggctgtattc ttttgggcca agttgtggac cacattgatt
6181 ctctcatgga agaatggttt cctgggttgc tggagattga tatttgtggt gaaggagaaa
6241 ctctgttgaa gaaatgggca ttatatagtt ttaatgatgg cgaagaacat caaaaaatct
6301 tacttgatga cttgatgaag aaagcagagg aaggagatct cttagtaaat ccagatcaac
6361 caaggctcac cattccaata tctcagattg cccctgactt gattttggct gacctgccta
6421 gaaatattat gttgaataat gatgagttgg aatttgaaca agctccagag tttctcctag
6481 gtgatggcag ttttggatca gtttaccgag cagcctatga aggagaagaa gtggctgtga
6541 agatttttaa taaacataca tcactcaggc tgttaagaca agagcttgtg gtgctttgcc
6601 acctccacca ccccagtttg atatctttgc tggcagctgg gattcgtccc cggatgttgg
6661 tgatggagtt agcctccaag ggttccttgg atcgcctgct tcagcaggac aaagccagcc
6721 tcactagaac cctacagcac aggatcgcac tccacgtagc tgatggtttg agatacctcc
6781 actcagccat gattatatac cgagccctga aaccccacaa tgtgctgctt ttcacactgt
6841 atcccaatgc tgccatcatc gcaaagattg ctgactacgg cattgctcag tactgctgta
6901 gaatggggat aaaaacatca gagggcacac cagggtttcg tgcacctgaa gttgccagag
6961 gaaatgtcat ttataaccaa caggctgatg tttattcatt tggtttacta ctctatgaca
7021 ttttgacaac tggaggtaga atagtagagg gtttgaagtt tccaaatgag tttgatgaat
7081 tagaaataca aggaaaatta cctgatccag ttaaagaata tggttgtgcc ccatggccta
7141 tggttgagaa attaattaaa cagtgtttga aagaaaatcc tcaagaaagg cctacttctg
7201 cccaggtctt tgacattttg aattcagctg aattagtctg tctgacgaga cgcattttat
7261 tacctaaaaa cgtaattgtt gaatgcatgg ttgctacaca tcacaacagc aggaatgcaa
7321 gcatttggct gggctgtggg cacaccgaca gaggacagct ctcatttctt gacttaaata
7381 ctgaaggata cacttctgag gaagttgctg atagtagaat attgtgctta gccttggtgc
7441 atcttcctgt tgaaaaggaa agctggattg tgtctgggac acagtctggt actctcctgg
7501 tcatcaatac cgaagatggg aaaaagagac ataccctaga aaagatgact gattctgtca
7561 cttgtttgta ttgcaattcc ttttccaagc aaagcaaaca aaaaaatttt cttttggttg
7621 gaaccgctga tggcaagtta gcaatttttg aagataagac tgttaagctt aaaggagctg
7681 ctcctttgaa gatactaaat ataggaaatg tcagtactcc attgatgtgt ttgagtgaat
7741 ccacaaattc aacggaaaga aatgtaatgt ggggaggatg tggcacaaag attttctcct
7801 tttctaatga tttcaccatt cagaaactca ttgagacaag aacaagccaa ctgttttctt
7861 atgcagcttt cagtgattcc aacatcataa cagtggtggt agacactgct ctctatattg
7921 ctaagcaaaa tagccctgtt gtggaagtgt gggataagaa aactgaaaaa ctctgtggac
7981 taatagactg cgtgcacttt ttaagggagg taatggtaaa agaaaacaag gaatcaaaac
8041 acaaaatgtc ttattctggg agagtgaaaa ccctctgcct tcagaagaac actgctcttt
8101 ggataggaac tggaggaggc catattttac tcctggatct ttcaactcgt cgacttatac
8161 gtgtaattta caacttttgt aattcggtca gagtcatgat gacagcacag ctaggaagcc
8221 ttaaaaatgt catgctggta ttgggctaca accggaaaaa tactgaaggt acacaaaagc
8281 agaaagagat acaatcttgc ttgaccgttt gggacatcaa tcttccacat gaagtgcaaa
8341 atttagaaaa acacattgaa gtgagaaaag aattagctga aaaaatgaga cgaacatctg
8401 ttgagtaaga gagaagggcg aattcgaccc agctttcttg tacaaagtgg tgatcggtac
8461 cttaattaat taaggtacca ggtaagtgta cccaattcgc cctatagtga gtcgtattac
8521 aattcactcg atcgcccttc ccaacagttg cgcagcctga atggcgaatg gagatccaat
8581 ttttaagtgt ataatgtgtt aaactactga ttctaattgt ttgtgtattt tagattcaca
8641 gtcccaaggc tcatttcagg cccctcagtc ctcacagtct gttcatgatc ataatcagcc
8701 ataccacatt tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc
8761 tgaaacataa aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt
8821 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
8881 gttgtggttt gtccaaactc atcaatgtat cttaacgcgt aaattgtaag cgttaatatt
8941 ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa
9001 atcggcaaaa tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca
9061 gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc
9121 gtctatcagg gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg
9181 aggtgccgta aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg
9241 ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg
9301 gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg
9361 ccgctacagg gcgcgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
9421 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
9481 cttcaataat attgaaaaag gaagaatcct gaggcggaaa gaaccagctg tggaatgtgt
9541 gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
9601 atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta
9661 tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc
9721 cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta
9781 tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct
9841 tttttggagg cctaggcttt tgcaaagatc gatcaagaga caggatgagg atcgtttcgc
9901 atgattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc
9961 ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca
10021 gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg
10081 caagacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg
10141 ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag
10201 gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg
10261 cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc
10321 atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa
10381 gaacatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgag catgcccgac
10441 ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat
10501 ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac
10561 atagcgttgg ctacccgtga tattgctgaa gaacttggcg gcgaatgggc tgaccgcttc
10621 ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt
10681 gacgagttct tctgagcggg actctggggt tcgaaatgac cgaccaagcg acgcccaacc
10741 tgccatcacg agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg
10801 ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg
10861 cccaccctag ggggaggcta actgaaacac ggaaggagac aataccggaa ggaacccgcg
10921 ctatgacggc aataaaaaga cagaataaaa cgcacggtgt tgggtcgttt gttcataaac
10981 gcggggttcg gtcccagggc tggcactctg tcgatacccc accgagaccc cattggggcc
11041 aatacgcccg cgtttcttcc ttttccccac cccacccccc aagttcgggt gaaggcccag
11101 ggctcgcagc caacgtcggg gcggcaggcc ctgccatagc ctcaggttac tcatatatac
11161 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
11221 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
11281 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
11341 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
11401 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
11461 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
11521 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
11581 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
11641 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
11701 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
11761 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
11821 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
11881 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
11941 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggata
//
