| 质粒名称: | pAAVLINKv2-SCP1-Cre-UDeg3a-LAMA1-C-FLAG |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001746 |
| 产品用途: | 3' AAVLINK plasmid for LAMA1 expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒 |
| 启动子: | SCP1 |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | LAMA1 |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
pAAVLINKv2-SCP1-Cre-UDeg3a-LAMA1-C-FLAG
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAVLINKv2-SCP1-Cre-UDeg3a-LAMA1-C-FLAG载体质粒基本信息
pAAVLINKv2-SCP1-Cre-UDeg3a-LAMA1-C-FLAG质粒图谱载体图谱和pAAVLINKv2-SCP1-Cre-UDeg3a-LAMA1-C-FLAG载体序列质粒序列多克隆位点信息
pAAVLINKv2-SCP1-Cre-UDeg3a-LAMA1-C-FLAG质粒载体简介
pAAVLINKv2-SCP1-Cre-UDeg3a-LAMA1-C-FLAG质粒序列载体序列
LOCUS pAAVLINKv2-SCP1-Cre-UDeg3a-LAMA1-C-FLAG 7189 bp DNA circular SYN 26-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7189)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported Jun 5, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..7189
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
misc_feature 145..225
/label=SCP1
CDS 237..1265
/codon_start=1
/product="site-specific recombinase"
/label=Cre
/note="Cre is a site-specific recombinase from
bacteriophage P1. Recombination occurs at loxP sequences."
/translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
DGD"
CDS 1281..1370
/codon_start=1
/label=UDeg3a
/translation="LLLKLLLLGGGSGPRPRGTRGKGRRIHYKG"
misc_feature 1295..1300
/label=SDS
misc_feature 1298..1303
/label=SDS
intron 1374..1414
/label=Splicing Donor
protein_bind 1421..1454
/label=lox2272-JTZ17
/bound_moiety="Cre recombinase"
/note="Right element (RE) mutant of loxP (Araki et al.,
2010). Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
intron 1455..1546
/label=Splicing Acceptor
/note="Splicing acceptor"
CDS 1547..3902
/codon_start=1
/label=LAMA1
/translation="GRQVPWVLRKLPE*RPFLPF*REWVLCRGEVTSGYRDPDNHAF*Y
LFT*WTSSLPGFIRHKRLFIHRAVSWQSEGYD*PGFRTHYPFDRQTL*QWNLVQNCLPA
KPEARSASSYRCL*HQ**RNQAGRDSGSIF*PQPPRQGPDLCGWITKVKSCKERCHHQK
LCGLHQEPGNIQINL*LTQKFLWSEKRLFTGAHPEC*LPERRLH*IATQIFVTRIRMAG
NICHHEQQWHHPGCPRRGCGEAG*S*GSTRALLFRHADRRQH*GTCQSWGWDRPEKSSP
ARSHGYLQ*WTSAFHLLGQESENYHCPIG*EQSCGNEVGHISRKQDDKCVQSVRRGNSR
GRGDVTAHNEKIVPWLYQKPDLQFGTFGFQQCSWP*ASRPGHLLAVRKA*AGSRCRGQQ
ALARAPGFSRTVCGGCSSGVRSRRSPVWSHTKQPFHLAF*SVGCQKEALG*AKHPHVRL
QRPDLLHGSSEPSRLRCAPAARGPPPLHV*PWQRQNKGLSPCTAQ*WQVAHGQDRLC*K
KRLHNCRRPRVSHGDCGGRWNHAGCGGFVLPRRPALPVPGQENWKYHPQHPCLHWGCDG
*QQTAGQGQPGVCLHGEQVLRSGPGRNIL*RKRICSSCQRGLQSPVRCEHHTGVSNLLA
EWRPPGDQHCQSGCHWTRACGRQGLVPCQQWCWQDNSCI*AQNRHCAL*WKMAHSSS*Q
KQTPYHSDC*RERSWR*KSTHPVYLSGHQQSHLCWWLSCWCEAKMPAQPDLVPRVFEEA
SSD*EPAGAVL*LQQSVRTARSFPSFLSWDRV"
misc_feature 3191..3196
/label=SDS
misc_feature 3442..3447
/label=SDS
misc_feature 3461..3466
/label=SDS
CDS 3909..3974
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
terminator 3978..4409
/label=W3SL
/note="Short WPRE and SV40 polyA"
misc_feature 4122..4127
/label=SDS
repeat_region 4452..4592
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 4452..4581
/label=AAV2 ITR
rep_origin 4667..5122
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 5404..5508
/gene="bla"
/label=AmpR promoter
CDS 5509..6369
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 6540..7128
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtgtactt atataagggg gtgggggcgc gttcgtcctc
181 agtcgcgatc gaacactcga gccgagcaga cgtgcctacg gaccgcgtac gccaccatgt
241 ccaatttact gaccgtacac caaaatttgc ctgcattacc ggtcgatgca acgagtgatg
301 aggttcgcaa gaacctgatg gacatgttca gggatcgcca ggcgttttct gagcatacct
361 ggaaaatgct tctgtccgtt tgccggtcgt gggcggcatg gtgcaagttg aataaccgga
421 aatggtttcc cgcagaacct gaagatgttc gcgattatct tctatatctt caggcgcgcg
481 gtctggcagt aaaaaccatc cagcaacatt tgggccagct aaacatgctt catcgtcggt
541 ccgggctgcc acgaccaagt gacagcaatg ctgtttcact ggttatgcgg cggatccgaa
601 aagaaaacgt tgatgccggt gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt
661 tcgaccaggt tcgttcactc atggaaaata gcgatcgctg ccaggatata cgtaatctgg
721 catttctggg gattgcttat aacaccctgt tacgtatagc cgaaattgcc aggatcaggg
781 ttaaagatat ctcacgtact gacggtggga gaatgttaat ccatattggc agaacgaaaa
841 cgctggttag caccgcaggt gtagagaagg cacttagcct gggggtaact aaactggtcg
901 agcgatggat ttccgtctct ggtgtagctg atgatccgaa taactacctg ttttgccggg
961 tcagaaaaaa tggtgttgcc gcgccatctg ccaccagcca gctatcaact cgcgccctgg
1021 aagggatttt tgaagcaact catcgattga tttacggcgc taaggatgac tctggtcaga
1081 gatacctggc ctggtctgga cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg
1141 ctggagtttc aataccggag atcatgcaag ctggtggctg gaccaatgta aatattgtaa
1201 tgaactatat ccgtaacctg gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg
1261 gcgatggagg tggatctggt ctgctgctga aactcctcct cctcggtggt ggttctggtc
1321 ctcgtccccg tggtactcgt ggtaaaggtc gccgtattca ctacaaagga taagtaagta
1381 tcaaggttac aagacaggtt taaggagacc aataactagt ataacttcgt ataggatact
1441 ttatagcaat ttatgaaact gggcttgtcg agacagagaa gactcttgcg tttctgatag
1501 gcacctattg gtcttactga catccacttt gcctttctct ccacagggaa ggcaagtgcc
1561 gtgggtgctt cggaagctcc cagaatgaag acccttcctt ccattttgac gggagtgggt
1621 actctgtcgt ggagaagtca cttccggcta ccgtgaccca gataatcatg ctttttaata
1681 ccttttcacc taatggactt cttctctacc tgggttcata cggcacaaaa gactttttat
1741 ccatcgagct gtttcgtggc agagtgaagg ttatgactga cctgggttca ggacccatta
1801 cccttttgac agacagacgt tataacaatg gaacctggta caaaattgcc ttccagcgaa
1861 accggaagca aggagtgcta gcagttatcg atgcctataa caccagtaat aaagaaacca
1921 agcagggcga gactccggga gcatcttctg acctcaaccg cctagacaag gacccgattt
1981 atgtgggtgg attaccaagg tcaagagttg taaggagagg tgtcaccacc aaaagctttg
2041 tgggctgcat caagaacctg gaaatatcca gatcaacctt tgacttactc agaaattcct
2101 atggagtgag aaaaggctgt ttactggagc ccatccggag tgttagcttc ctgaaaggcg
2161 gctacattga attgccaccc aaatctttgt caccagaatc agaatggctg gtaacatttg
2221 ccaccacgaa cagcagtggc atcatcctgg ctgccctcgg cggggatgtg gagaagcggg
2281 gtgatcgtga ggaagcacac gtgcccttct tttccgtcat gctgatcgga ggcaacattg
2341 aggtacatgt caatcctggg gatgggacag gcctgagaaa agctctcctg cacgctccca
2401 cgggtacctg cagtgatgga caagcgcatt ccatctcctt ggtcaggaat cggagaatta
2461 tcactgtcca attggatgag aacaatcctg tggaaatgaa gttgggcaca ttagtagaaa
2521 gcaggacgat aaatgtgtcc aatctgtacg tcgggggaat tccagaggga gaggggacgt
2581 cactgctcac aatgagaaga tcgttccatg gctgtatcaa aaacctgatc ttcaatttgg
2641 aacttttgga tttcaacagt gcagttggcc atgagcaagt cgacctggac acctgctggc
2701 tgtcagaaag gcctaagctg gctcccgatg cagaggacag caagctcttg ccagagcccc
2761 gggcttttcc agaacagtgt gtggtggatg cagctctgga gtacgttccc ggcgctcacc
2821 agtttggtct cacacaaaac agccatttca tcttgccttt taatcagtcg gctgtcagaa
2881 agaagctctc ggttgagcta agcatccgca cgttcgcctc cagcggcctg atttactaca
2941 tggctcatca gaaccaagca gactacgctg tgctccagct gcacgggggc cgcctccact
3001 tcatgtttga ccttggcaaa ggcagaacaa aggtctctca ccctgcactg ctcagtgatg
3061 gcaagtggca cacggtcaag acagactatg ttaaaagaaa aggcttcata actgtcgacg
3121 gccgagagtc tcccatggtg actgtggtgg gagatggaac catgctggat gtggagggtt
3181 tgttctacct aggaggcctg ccctcccagt accaggccag gaaaattgga aatatcaccc
3241 acagcatccc tgcctgcatt ggggatgtga cggttaacag caaacagctg gacaaggaca
3301 gcccggtgtc tgccttcacg gtgaacaggt gctacgcagt ggcccaggaa ggaacatact
3361 ttgacggaag cggatatgca gctcttgtca aagagggcta caaagtccag tcagatgtga
3421 acatcacact ggagtttcga acctcctcgc agaatggcgt cctcctgggg atcagcactg
3481 ccaaagtgga tgccattgga ctagagcttg tggacggcaa ggtcttgttc catgtcaaca
3541 atggtgctgg caggataaca gctgcatatg agcccaaaac cgccactgtg ctctgtgatg
3601 gaaaatggca cactcttcaa gctaacaaaa gcaaacaccg tatcactctg attgttgacg
3661 ggaacgcagt tggcgctgaa agtccacaca cccagtctac ctcagtggac accaacaatc
3721 ccatttatgt tggtggctat cctgctggtg tgaagcaaaa atgcctgcgc agccagacct
3781 cgttccgcgg gtgtttgagg aagctagctc tgattaagag cccgcaggtg cagtcctttg
3841 acttcagcag agcgttcgaa ctgcacggag ttttccttca ttcctgtcct gggaccgagt
3901 cctgtacaga ctacaaagac catgacggtg attataaaga tcatgacatc gactacaagg
3961 atgacgatga caagtaactc gagataatca acctctggat tacaaaattt gtgaaagatt
4021 gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc
4081 tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg
4141 gttagttctt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc
4201 tcggctgttg ggcactgaca attccgtggt gtttatttgt gaaatttgtg atgctattgc
4261 tttatttgta accatctagc tttatttgtg aaatttgtga tgctattgct ttatttgtaa
4321 ccattataag ctgcaataaa caagttaaca acaacaattg cattcatttt atgtttcagg
4381 ttcaggggga gatgtgggag gttttttaaa gatctggaga ccggtgtggc acgtgcggac
4441 cgagcggccg caggaacccc tagtgatgga gttggccact ccctctctgc gcgctcgctc
4501 gctcactgag gccgggcgac caaaggtcgc ccgacgcccg ggctttgccc gggcggcctc
4561 agtgagcgag cgagcgcgca gctgcctgca ggggcgcctg atgcggtatt ttctccttac
4621 gcatctgtgc ggtatttcac accgcatacg tcaaagcaac catagtacgc gccctgtagc
4681 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc
4741 gccttagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt
4801 ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac
4861 ctcgacccca aaaaacttga tttgggtgat ggttcacgta gtgggccatc gccctgatag
4921 acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa
4981 actggaacaa cactcaactc tatctcgggc tattcttttg atttataagg gattttgccg
5041 atttcggtct attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac
5101 aaaatattaa cgtttacaat tttatggtgc actctcagta caatctgctc tgatgccgca
5161 tagttaagcc agccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg
5221 ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg
5281 ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg cctattttta
5341 taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat
5401 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
5461 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
5521 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
5581 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
5641 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
5701 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc
5761 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
5821 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
5881 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
5941 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
6001 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg
6061 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
6121 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
6181 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
6241 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
6301 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
6361 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
6421 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
6481 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
6541 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
6601 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
6661 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
6721 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
6781 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
6841 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
6901 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
6961 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
7021 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
7081 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
7141 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgt
//
