| 质粒名称: | pCMVR8.74 |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 22036 |
| 存储实验室: | Didier Trono |
|
载体骨架基本信息 |
|
| 载体名称: | pCMV |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 4661 bp |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | gag pol tat rev |
| 物种来源: | HIV-1 |
| 插入大小: | 5765 bp |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | -- |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pCMVR8.74 was a gift from Didier Trono (Addgene plasmid # 22036 ; http://n2t.net/addgene:22036 ; RRID:Addgene_22036) |
买家导航
pCMVR8.74载体质粒基本信息
pCMVR8.74质粒图谱载体图谱和pCMVR8.74载体序列质粒序列多克隆位点信息
pCMVR8.74质粒载体简介
pCMVR8.74质粒序列载体序列
LOCUS pCMVR8.74 11904 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION 2nd generation lentiviral packaging plasmid. Can be used with 2nd or
3rd generation lentiviral vectors and envelope expressing plasmid
(Addgene#12259).
ACCESSION .
VERSION .
KEYWORDS pCMVR8.74
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 11904)
TITLE TRONO lab plasmids
REFERENCE 2 (bases 1 to 11904)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..11904
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind 32..51
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 285..302
/label=L4440
/note="L4440 vector, forward primer"
primer_bind complement(372..392)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
promoter 377..706
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 557..692
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 619..638
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
intron 1840..1905
/label=small t intron
/note="SV40 (simian virus 40) small t antigen intron"
CDS 2035..2055
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
enhancer 2509..2888
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 2889..3092
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 3043..3063
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
primer_bind 3089..3113
/label=LNCX
/note="Human CMV promoter, forward primer"
CDS 3230..4732
/codon_start=1
/gene="gag"
/product="gag protein from human immunodeficiency virus 1"
/label=HIV-1 gag
/translation="MGARASVLSGGELDRWEKIRLRPGGKKKYKLKHIVWASRELERFA
VNPGLLETSEGCRQILGQLQPSLQTGSEELRSLYNTVATLYCVHQRIEIKDTKEALDKI
EEEQNKSKKKAQQAAADTGHSNQVSQNYPIVQNIQGQMVHQAISPRTLNAWVKVVEEKA
FSPEVIPMFSALSEGATPQDLNTMLNTVGGHQAAMQMLKETINEEAAEWDRVHPVHAGP
IAPGQMREPRGSDIAGTTSTLQEQIGWMTHNPPIPVGEIYKRWIILGLNKIVRMYSPTS
ILDIRQGPKEPFRDYVDRFYKTLRAEQASQEVKNWMTETLLVQNANPDCKTILKALGPG
ATLEEMMTACQGVGGPGHKARVLAEAMSQVTNPATIMIQKGNFRNQRKTVKCFNCGKEG
HIAKNCRAPRKKGCWKCGKEGHQMKDCTERQANFLGKIWPSHKGRPGNFLQSRPEPTAP
PEESFRFGEETTTPSQKQEPIDKELYPLASLRSLFGSDPSSQ"
CDS 4525..7536
/codon_start=1
/gene="pol"
/product="pol protein from human immunodeficiency virus 1"
/label=HIV-1 pol
/translation="FFREDLAFPQGKAREFSSEQTRANSPTRRELQVWGRDNNSLSEAG
ADRQGTVSFSFPQITLWQRPLVTIKIGGQLKEALLDTGADDTVLEEMNLPGRWKPKMIG
GIGGFIKVRQYDQILIEICGHKAIGTVLVGPTPVNIIGRNLLTQIGCTLNFPISPIETV
PVKLKPGMDGPKVKQWPLTEEKIKALVEICTEMEKEGKISKIGPENPYNTPVFAIKKKD
STKWRKLVDFRELNKRTQDFWEVQLGIPHPAGLKQKKSVTVLDVGDAYFSVPLDKDFRK
YTAFTIPSINNETPGIRYQYNVLPQGWKGSPAIFQCSMTKILEPFRKQNPDIVIYQYMD
DLYVGSDLEIGQHRTKIEELRQHLLRWGFTTPDKKHQKEPPFLWMGYELHPDKWTVQPI
VLPEKDSWTVNDIQKLVGKLNWASQIYAGIKVRQLCKLLRGTKALTEVVPLTEEAELEL
AENREILKEPVHGVYYDPSKDLIAEIQKQGQGQWTYQIYQEPFKNLKTGKYARMKGAHT
NDVKQLTEAVQKIATESIVIWGKTPKFKLPIQKETWEAWWTEYWQATWIPEWEFVNTPP
LVKLWYQLEKEPIIGAETFYVDGAANRETKLGKAGYVTDRGRQKVVPLTDTTNQKTELQ
AIHLALQDSGLEVNIVTDSQYALGIIQAQPDKSESELVSQIIEQLIKKEKVYLAWVPAH
KGIGGNEQVDKLVSAGIRKVLFLDGIDKAQEEHEKYHSNWRAMASDFNLPPVVAKEIVA
SCDKCQLKGEAMHGQVDCSPGIWQLDCTHLEGKVILVAVHVASGYIEAEVIPAETGQET
AYFLLKLAGRWPVKTVHTDNGSNFTSTTVKAACWWAGIKQEFGIPYNPQSQGVIESMNK
ELKKIIGQVRDQAEHLKTAVQMAVFIHNFKRKGGIGGYSAGERIVDIIATDIQTKELQK
QITKIQNFRVYYRDSRDPVWKGPAKLLWKGEGAVVIQDNSDIKVVPRRKAKIIRDYGKQ
MAGDDCVASRQDED"
misc_feature 7221..7338
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
misc_feature 8110..8343
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
CDS 8866..8892
/codon_start=1
/product="nuclear export signal from the HIV Rev protein
(Fischer et al., 1995)"
/label=NES
/translation="LPPLERLTL"
promoter complement(9505..9523)
/label=SP6 promoter
/note="promoter for bacteriophage SP6 RNA polymerase"
primer_bind complement(9506..9523)
/label=SP6
/note="SP6 promoter, forward primer"
primer_bind complement(9813..9832)
/label=pBRrevBam
/note="pBR322 vectors, tet region, downstream of BamHI,
reverse primer"
primer_bind complement(10046..10065)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind 10165..10187
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
promoter 10311..10415
/gene="bla"
/label=AmpR promoter
CDS 10416..11276
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(10634..10653)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 11447..131
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
61 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
121 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
181 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
241 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
301 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
361 aatgcagctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg
421 cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg
481 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc
541 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca
601 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt
661 ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc ttggacacaa
721 gacaggcttg cgagatatgt ttgagaatac cactttatcc cgcgtcaggg agaggcagtg
781 cgtaaaaaga cgcggactca tgtgaaatac tggtttttag tgcgccagat ctctataatc
841 tcgcgcaacc tattttcccc tcgaacactt tttaagccgt agataaacag gctgggacac
901 ttcacatgag cgaaaaatac atcgtcacct gggacatgtt gcagatccat gcacgtaaac
961 tcgcaagccg actgatgcct tctgaacaat ggaaaggcat tattgccgta agccgtggcg
1021 gtctgtaccg ggtgcgttac tggcgcgtga actgggtatt cgtcatgtcg ataccgtttg
1081 tatttccagc tacgatcacg acaaccagcg cgagcttaaa gtgctgaaac gcgcagaagg
1141 cgatggcgaa ggcttcatcg ttattgatga cctggtggat accggtggta ctgcggttgc
1201 gattcgtgaa atgtatccaa aagcgcactt tgtcaccatc ttcgcaaaac cggctggtcg
1261 tccgctggtt gatgactatg ttgttgatat cccgcaagat acctggattg aacagccgtg
1321 ggatatgggc gtcgtattcg tcccgccaat ctccggtcgc taatcttttc aacgcctggc
1381 actgccgggc gttgttcttt ttaacttcag gcgggttaca atagtttcca gtaagtattc
1441 tggaggctgc atccatgaca caggcaaacc tgagcgaaac cctgttcaaa ccccgcttta
1501 aacatcctga aacctcgacg ctagtccgcc gctttaatca cggcgcacaa ccgcctgtgc
1561 agtcggccct tgatggtaaa accatccctc actggtatcg catgattaac cgtctgatgt
1621 ggatctggcg cggcattgac ccacgcgaaa tcctcgacgt ccaggcacgt attgtgatga
1681 gcgatgccga acgtaccgac gatgatttat acgatacggt gattggctac cgtggcggca
1741 actggattta tgagtgggcc ccggatcttt gtgaaggaac cttacttctg tggtgtgaca
1801 taattggaca aactacctac agagatttaa agctctaagg taaatataaa atttttaagt
1861 gtataatgtg ttaaactact gattctaatt gtttgtgtat tttagattcc aacctatgga
1921 actgatgaat gggagcagtg gtggaatgcc tttaatgagg aaaacctgtt ttgctcagaa
1981 gaaatgccat ctagtgatga tgaggctact gctgactctc aacattctac tcctccaaaa
2041 aagaagagaa aggtagaaga ccccaaggac tttccttcag aattgctaag ttttttgagt
2101 catgctgtgt ttagtaatag aactcttgct tgctttgcta tttacaccac aaaggaaaaa
2161 gctgcactgc tatacaagaa aattatggaa aaatattctg taacctttat aagtaggcat
2221 aacagttata atcataacat actgtttttt cttactccac acaggcatag agtgtctgct
2281 attaataact atgctcaaaa attgtgtacc tttagctttt taatttgtaa aggggttaat
2341 aaggaatatt tgatgtatag tgccttgact agagatcata atcagccata ccacatttgt
2401 agaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga aacataaaat
2461 gaatgcaatt gttgttgttg ggctgcagga attaattcga gctcgcccga cattgattat
2521 tgactagtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt
2581 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc
2641 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac
2701 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata
2761 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc
2821 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta
2881 ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac
2941 ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc
3001 aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc
3061 gtgtacggtg ggaggtctat ataagcagag ctcgtttagt gaaccgtcag atcgcctgga
3121 gacgccatcc acgctgtttt gacctccata gaagacaccg ggaccgatcc agcctccgcg
3181 gccgcgttga cgcgcacggc aagaggcgag gggcggcgac tggtgagaga tgggtgcgag
3241 agcgtcagta ttaagcgggg gagaattaga tcgatgggaa aaaattcggt taaggccagg
3301 gggaaagaaa aaatataaat taaaacatat agtatgggca agcagggagc tagaacgatt
3361 cgcagttaat cctggcctgt tagaaacatc agaaggctgt agacaaatac tgggacagct
3421 acaaccatcc cttcagacag gatcagaaga acttagatca ttatataata cagtagcaac
3481 cctctattgt gtgcatcaaa ggatagagat aaaagacacc aaggaagctt tagacaagat
3541 agaggaagag caaaacaaaa gtaagaaaaa agcacagcaa gcagcagctg acacaggaca
3601 cagcaatcag gtcagccaaa attaccctat agtgcagaac atccaggggc aaatggtaca
3661 tcaggccata tcacctagaa ctttaaatgc atgggtaaaa gtagtagaag agaaggcttt
3721 cagcccagaa gtgataccca tgttttcagc attatcagaa ggagccaccc cacaagattt
3781 aaacaccatg ctaaacacag tggggggaca tcaagcagcc atgcaaatgt taaaagagac
3841 catcaatgag gaagctgcag aatgggatag agtgcatcca gtgcatgcag ggcctattgc
3901 accaggccag atgagagaac caaggggaag tgacatagca ggaactacta gtacccttca
3961 ggaacaaata ggatggatga cacataatcc acctatccca gtaggagaaa tctataaaag
4021 atggataatc ctgggattaa ataaaatagt aagaatgtat agccctacca gcattctgga
4081 cataagacaa ggaccaaagg aaccctttag agactatgta gaccgattct ataaaactct
4141 aagagccgag caagcttcac aagaggtaaa aaattggatg acagaaacct tgttggtcca
4201 aaatgcgaac ccagattgta agactatttt aaaagcattg ggaccaggag cgacactaga
4261 agaaatgatg acagcatgtc agggagtggg gggacccggc cataaagcaa gagttttggc
4321 tgaagcaatg agccaagtaa caaatccagc taccataatg atacagaaag gcaattttag
4381 gaaccaaaga aagactgtta agtgtttcaa ttgtggcaaa gaagggcaca tagccaaaaa
4441 ttgcagggcc cctaggaaaa agggctgttg gaaatgtgga aaggaaggac accaaatgaa
4501 agattgtact gagagacagg ctaatttttt agggaagatc tggccttccc acaagggaag
4561 gccagggaat tttcttcaga gcagaccaga gccaacagcc ccaccagaag agagcttcag
4621 gtttggggaa gagacaacaa ctccctctca gaagcaggag ccgatagaca aggaactgta
4681 tcctttagct tccctcagat cactctttgg cagcgacccc tcgtcacaat aaagataggg
4741 gggcaattaa aggaagctct attagataca ggagcagatg atacagtatt agaagaaatg
4801 aatttgccag gaagatggaa accaaaaatg atagggggaa ttggaggttt tatcaaagta
4861 agacagtatg atcagatact catagaaatc tgcggacata aagctatagg tacagtatta
4921 gtaggaccta cacctgtcaa cataattgga agaaatctgt tgactcagat tggctgcact
4981 ttaaattttc ccattagtcc tattgagact gtaccagtaa aattaaagcc aggaatggat
5041 ggcccaaaag ttaaacaatg gccattgaca gaagaaaaaa taaaagcatt agtagaaatt
5101 tgtacagaaa tggaaaagga aggaaaaatt tcaaaaattg ggcctgaaaa tccatacaat
5161 actccagtat ttgccataaa gaaaaaagac agtactaaat ggagaaaatt agtagatttc
5221 agagaactta ataagagaac tcaagatttc tgggaagttc aattaggaat accacatcct
5281 gcagggttaa aacagaaaaa atcagtaaca gtactggatg tgggcgatgc atatttttca
5341 gttcccttag ataaagactt caggaagtat actgcattta ccatacctag tataaacaat
5401 gagacaccag ggattagata tcagtacaat gtgcttccac agggatggaa aggatcacca
5461 gcaatattcc agtgtagcat gacaaaaatc ttagagcctt ttagaaaaca aaatccagac
5521 atagtcatct atcaatacat ggatgatttg tatgtaggat ctgacttaga aatagggcag
5581 catagaacaa aaatagagga actgagacaa catctgttga ggtggggatt taccacacca
5641 gacaaaaaac atcagaaaga acctccattc ctttggatgg gttatgaact ccatcctgat
5701 aaatggacag tacagcctat agtgctgcca gaaaaggaca gctggactgt caatgacata
5761 cagaaattag tgggaaaatt gaattgggca agtcagattt atgcagggat taaagtaagg
5821 caattatgta aacttcttag gggaaccaaa gcactaacag aagtagtacc actaacagaa
5881 gaagcagagc tagaactggc agaaaacagg gagattctaa aagaaccggt acatggagtg
5941 tattatgacc catcaaaaga cttaatagca gaaatacaga agcaggggca aggccaatgg
6001 acatatcaaa tttatcaaga gccatttaaa aatctgaaaa caggaaagta tgcaagaatg
6061 aagggtgccc acactaatga tgtgaaacaa ttaacagagg cagtacaaaa aatagccaca
6121 gaaagcatag taatatgggg aaagactcct aaatttaaat tacccataca aaaggaaaca
6181 tgggaagcat ggtggacaga gtattggcaa gccacctgga ttcctgagtg ggagtttgtc
6241 aatacccctc ccttagtgaa gttatggtac cagttagaga aagaacccat aataggagca
6301 gaaactttct atgtagatgg ggcagccaat agggaaacta aattaggaaa agcaggatat
6361 gtaactgaca gaggaagaca aaaagttgtc cccctaacgg acacaacaaa tcagaagact
6421 gagttacaag caattcatct agctttgcag gattcgggat tagaagtaaa catagtgaca
6481 gactcacaat atgcattggg aatcattcaa gcacaaccag ataagagtga atcagagtta
6541 gtcagtcaaa taatagagca gttaataaaa aaggaaaaag tctacctggc atgggtacca
6601 gcacacaaag gaattggagg aaatgaacaa gtagataaat tggtcagtgc tggaatcagg
6661 aaagtactat ttttagatgg aatagataag gcccaagaag aacatgagaa atatcacagt
6721 aattggagag caatggctag tgattttaac ctaccacctg tagtagcaaa agaaatagta
6781 gccagctgtg ataaatgtca gctaaaaggg gaagccatgc atggacaagt agactgtagc
6841 ccaggaatat ggcagctaga ttgtacacat ttagaaggaa aagttatctt ggtagcagtt
6901 catgtagcca gtggatatat agaagcagaa gtaattccag cagagacagg gcaagaaaca
6961 gcatacttcc tcttaaaatt agcaggaaga tggccagtaa aaacagtaca tacagacaat
7021 ggcagcaatt tcaccagtac tacagttaag gccgcctgtt ggtgggcggg gatcaagcag
7081 gaatttggca ttccctacaa tccccaaagt caaggagtaa tagaatctat gaataaagaa
7141 ttaaagaaaa ttataggaca ggtaagagat caggctgaac atcttaagac agcagtacaa
7201 atggcagtat tcatccacaa ttttaaaaga aaagggggga ttggggggta cagtgcaggg
7261 gaaagaatag tagacataat agcaacagac atacaaacta aagaattaca aaaacaaatt
7321 acaaaaattc aaaattttcg ggtttattac agggacagca gagatccagt ttggaaagga
7381 ccagcaaagc tcctctggaa aggtgaaggg gcagtagtaa tacaagataa tagtgacata
7441 aaagtagtgc caagaagaaa agcaaagatc atcagggatt atggaaaaca gatggcaggt
7501 gatgattgtg tggcaagtag acaggatgag gattaacaca tggaattctg caacaactgc
7561 tgtttatcca tttcagaatt gggtgtcgac atagcagaat aggcgttact cgacagagga
7621 gagcaagaaa tggagccagt agatcctaga ctagagccct ggaagcatcc aggaagtcag
7681 cctaaaactg cttgtaccaa ttgctattgt aaaaagtgtt gctttcattg ccaagtttgt
7741 ttcatgacaa aagccttagg catctcctat ggcaggaaga agcggagaca gcgacgaaga
7801 gctcatcaga acagtcagac tcatcaagct tctctatcaa agcagtaagt agtacatgta
7861 atgcaaccta taatagtagc aatagtagca ttagtagtag caataataat agcaatagtt
7921 gtgtggtcca tagtaatcat agaatatagg aaaatggccg ctgatcttca gacctggagg
7981 aggagatatg agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga
8041 accattagga gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc
8101 agtgggaata ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc
8161 agcgtcaatg acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca
8221 gaacaatttg ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg
8281 catcaagcag ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct
8341 cctggggatt tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc
8401 tagttggagt aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga
8461 cagagaaatt aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca
8521 gcaagaaaag aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg
8581 gtttaacata acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt
8641 ggtaggttta agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata
8701 ttcaccatta tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg
8761 aatagaagaa gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc
8821 cttggcactt atctgggacg atctgcggag cctgtgcctc ttcagctacc accgcttgag
8881 agacttactc ttgattgtaa cgaggattgt ggaacttctg ggacgcaggg ggtgggaagc
8941 cctcaaatat tggtggaatc tcctacaata ttggagtcag gagctaaaga atagtgctgt
9001 tagcttgctc aatgccacag ccatagcagt agctgagggg acagataggg ttatagaagt
9061 agtacaagga gcttgtagag ctattcgcca catacctaga agaataagac agggcttgga
9121 aaggattttg ctataagctc gagtgacctt cagaccttgg cactggaggt ggcccggcag
9181 aagcgcggca tcgtggatca gtgctgcacc agcatctgct ctctctacca actggagaac
9241 tactgcaact aggcccacca ctaccctgtc cacccctctg caatgaataa aacctttgaa
9301 agagcactac aagttgtgtg tacatgcgtg catgtgcata tgtggtgcgg ggggaacatg
9361 agtggggctg gctggagtgg cgatgataag ctgtcaaaca tgagaattaa ttcttgaaga
9421 cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct
9481 tagtctagaa ttaattccgt gtattctata gtgtcaccta aatcgtatgt gtatgataca
9541 taaggttatg tattaattgt agccgcgttc taacgacaat atgtacaagc ctaattgtgt
9601 agcatctggc ttactgaagc agaccctatc atctctctcg taaactgccg tcagagtcgg
9661 tttggttgga cgaaccttct gagtttctgg taacgccgtc ccgcacccgg aaatggtcag
9721 cgaaccaatc agcagggtca tcgctagcca gatcctctac gccggacgca tcgtggccgg
9781 catcaccggc gccacaggtg cggttgctgg cgcctatatc gccgacatca ccgatgggga
9841 agatcgggct cgccacttcg ggctcatgag cgcttgtttc ggcgtgggta tggtggcagg
9901 ccccgtggcc gggggactgt tgggcgccat ctccttgcat gcaccattcc ttgcggcggc
9961 ggtgctcaac ggcctcaacc tactactggg ctgcttccta atgcaggagt cgcataaggg
10021 agagcgtcga atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagc
10081 cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg
10141 cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat
10201 caccgaaacg cgcgagacga aagggcctcg tgatacgcct atttttatag gttaatgtca
10261 tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc
10321 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
10381 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
10441 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
10501 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
10561 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
10621 cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac
10681 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
10741 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
10801 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
10861 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
10921 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc
10981 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
11041 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
11101 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
11161 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
11221 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
11281 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
11341 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
11401 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
11461 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
11521 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
11581 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
11641 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
11701 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
11761 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
11821 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
11881 ggtatccggt aagcggcagg gtcg
//
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