| 质粒名称: | pLYL171 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0002789 |
| 产品用途: | Mammalian PhCMV-driven constitutive expression vector for C-terminally ecDHFRN18T, A19V-tagged NanoLuc containing a C-terminal 6xHis-tag (PhCMV-NanoLuc-ecDHFRN18T, A19V-6xHis-tag-pA). |
| 存储实验室: | Mingqi XIE Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pcDNA3.1 |
| 表达系统: | 哺乳动物表达 |
| 启动子: | CMV |
| 拷贝数: | 未拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | Stbl3 |
|
插入基因信息 |
|
| 插入基因名称: | ecDHFRN18T, A19V/6xHis-tag, NanoLuc |
| 物种来源: | synthetic |
| 密码子优化: | 未优化 |
pLYL171
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pLYL171载体质粒基本信息
pLYL171质粒图谱载体图谱和pLYL171载体序列质粒序列多克隆位点信息
pLYL171质粒载体简介
pLYL171质粒序列载体序列
LOCUS pLYL171 6533 bp DNA circular SYN 15-JUL-2026
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6533)
AUTHORS Westlake University
TITLE Direct Submission
JOURNAL Exported Sep 12, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6533
/mol_type="other DNA"
/organism="synthetic DNA construct"
primer_bind 186..207
/label=olyl419
misc_feature 235..818
/label=P_CMV
/label=nonstandard type: promoter_const
misc_feature 235..614
/label=P_CMV_enhancer
/label=nonstandard type: promoter_element
misc_feature 615..818
/label=P_CMV_IE
/label=nonstandard type: promoter_element
primer_bind 769..789
/label=oMHS-CMV-F
misc_feature 829
/label=putative transcriptional start
promoter 863..882
/label=T7 promoter
primer_bind complement(871..894)
/label=olyl418
primer_bind 897..939
/label=olyl396
primer_bind 917..953
/label=oSCL1108
misc_feature 920..924
/label=NheI
primer_bind 930..953
/label=oSCL084
misc_feature 932..1000
/label=Secretory (IgK)
/label=nonstandard type: signal
primer_bind complement(996..1018)
/label=olyl490
misc_feature 1022..1531
/label=NLuc (nano Luciferase)
/label=nonstandard type: reporter
misc_feature 1052..1054
/label=cyt-STOP (TGG->TGA)
primer_bind complement(1517..1557)
/label=olyl460
primer_bind complement(1517..1545)
/label=olyl459
primer_bind complement(1521..1539)
/label=oSCL1109
primer_bind complement(1521..1537)
/label=oSCL085
primer_bind 1535..1574
/label=olyl339
primer_bind 1535..1574
/label=olyl345
primer_bind 1535..1550
/label=olyl404
primer_bind 1537..1574
/label=olyl347
primer_bind 1538..1558
/label=olyl461
primer_bind 1538..1558
/label=olyl494
CDS 1538..1552
/codon_start=1
/label=linker
/translation="GSGGG"
sig_peptide 1538..1539
/label=P2A
misc_feature 1559..2035
/label=ecDHFR(N18T/A19V)
primer_bind complement(2013..2076)
/label=olyl402
primer_bind complement(2013..2076)
/label=olyl495
CDS 2036..2053
/codon_start=1
/product="6xHis affinity tag"
/label=6xHis
/translation="HHHHHH"
primer_bind complement(2092..2115)
/label=olyl256
terminator 2130..2357
/label=bGH polyA
rep_origin 2403..2831
/label=f1 bacteriophage origin of replication
/label=f1 bacteriophage origin of replication; arrow _f1
ori
misc_feature 2845..3174
/label=P_SV40
/label=nonstandard type: promoter_const
rep_origin 3025..3160
/label=SV40 ORI
misc_feature 3077..3126
/label=P_SV40 TSS-region
CDS 3241..4035
/label=Translation 2136-2930
misc_feature 3241..4032
/label=Kan/neoR
/label=nonstandard type: resistance
primer_bind complement(4375..4395)
/label=M13-rev
misc_binding complement(4401..4423)
/label=LacO
promoter complement(4427..4457)
/label=lac promoter
rep_origin complement(4781..5366)
/direction=LEFT
/label=high-copy-number ColE1/pMB1/pBR322/pUC origin o
/label=high-copy-number ColE1/pMB1/pBR322/pUC origin of
_ori
CDS complement(5537..6397)
/label=Translation 5292-4432
misc_feature complement(5540..6397)
/label=AmpR
/label=nonstandard type: resistance
promoter complement(6398..6502)
/label=AmpR promoter
ORIGIN
1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
901 gtttaaactt aagcttctgg ctagcgccac catgactagt gagacagaca cactcctgct
961 atgggtactg ctgctctggg ttccaggttc cactggtgac gctagtggtg gttctggtat
1021 ggtcttcaca ctcgaagatt tcgttgggga ctggcgacag acagccggct acaacctgga
1081 ccaagtcctt gaacagggag gtgtgtccag tttgtttcag aatctcgggg tgtccgtaac
1141 tccgatccaa aggattgtcc tgagcggtga aaatgggctg aagatcgaca tccatgtcat
1201 catcccgtat gaaggtctga gcggcgacca aatgggccag atcgaaaaaa tttttaaggt
1261 ggtgtaccct gtggatgatc atcactttaa ggtgatcctg cactatggca cactggtaat
1321 cgacggggtt acgccgaaca tgatcgacta tttcggacgg ccgtatgaag gcatcgccgt
1381 gttcgacggc aaaaagatca ctgtaacagg gaccctgtgg aacggcaaca aaattatcga
1441 cgagcgcctg atcaaccccg acggctccct gctgttccga gtaaccatca acggagtgac
1501 cggctggcgg ctgtgcgaac gcattctggc ggctagcggt tccggaggtg gcgaattcat
1561 gatcagcctg atcgccgccc tggccgtgga ccacgtgatc ggcatggaga ccgtgatgcc
1621 ctggaacctg cccgccgacc tggcctggtt caagagaaac accctgaaca agcccgtgat
1681 catgggcaga cacacctggg agagcatcgg cagacccctg cccggcagaa agaacatcat
1741 cctgagcagc cagcccagca ccgacgacag agtgacctgg gtgaagagcg tggacgaggc
1801 catcgccgcc tgcggcgacg tgcccgagat catggtgatc ggcggcggca gagtgtacga
1861 gcagttcctg cccaaggccc agaagctgta cctgacccac atcgacgccg aggtggaggg
1921 cgacacccac ttccccgact acgagcccga cgactgggag agcgtgttca gcgagttcca
1981 cgacgccgac gcccagaaca gccacagcta ctgcttcgag atcctggaga gaagacacca
2041 ccaccaccac cactaagaat tctgcagata tccagcacag tggcggccgc tcgagtctag
2101 agggcccgtt taaacccgct gatcagcctc gactgtgcct tctagttgcc agccatctgt
2161 tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc
2221 ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg
2281 tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga
2341 tgcggtgggc tctatggctt ctgaggcgga aagaaccagc tggggctcta gggggtatcc
2401 ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac
2461 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc
2521 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt
2581 tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg
2641 gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag
2701 tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt
2761 ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt
2821 taacgcgaat taattctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc
2881 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag
2941 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
3001 atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct
3061 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctctgcctct
3121 gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctc
3181 ccgggagctt gtatatccat tttcggatct gatcaagaga caggatgagg atcgtttcgc
3241 atgattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc
3301 ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca
3361 gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg
3421 caggacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg
3481 ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag
3541 gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg
3601 cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc
3661 atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa
3721 gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg catgcccgac
3781 ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat
3841 ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac
3901 atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc
3961 ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt
4021 gacgagttct tctgagcggg actctggggt tcgaaatgac cgaccaagcg acgcccaacc
4081 tgccatcacg agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg
4141 ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg
4201 cccaccccaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
4261 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
4321 atgtatctta tcatgtctgt ataccgtcga cctctagcta gagcttggcg taatcatggt
4381 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg
4441 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt
4501 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg
4561 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg
4621 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
4681 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
4741 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
4801 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
4861 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
4921 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
4981 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
5041 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
5101 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
5161 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
5221 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
5281 gctcttgatc cggcaaacaa accaccgctg gtagcggttt ttttgtttgc aagcagcaga
5341 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
5401 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
5461 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
5521 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
5581 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
5641 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
5701 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
5761 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
5821 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
5881 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
5941 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
6001 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
6061 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
6121 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
6181 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
6241 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
6301 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
6361 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
6421 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
6481 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtc
//
pLYL171载体图谱质粒图谱pdf版和pLYL171载体序列质粒序列等相关资料下载
pLYL171质粒载体应用举例
- 上一篇：pLYL176
- 下一篇：没有了
