pAAVLINKv1-pCALM1-Cre-3'-NEO1-FLAG

LOCUS       pAAVLINKv1-pCALM1-Cre-3'-NEO1-FLAG                7108 bp DNA     circular SYN 24-NOV-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7108)
  AUTHORS   Stavrini Papadaki
  TITLE     Direct Submission
  JOURNAL   Exported Jun 10, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..7108
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
                     /note="color: #ffe4c4"
     promoter        145..265
                     /label=pCALM1
     CDS             277..1308
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
                     LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
                     PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
                     LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
                     RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
                     RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
                     DGD"
     intron          1309..1349
                     /label=Splicing Donor
     protein_bind    1356..1389
                     /label=lox2272-JTZ17
                     /bound_moiety="Cre recombinase"
                     /note="Right element (RE) mutant of loxP (Araki et al., 
                     2010). Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     intron          1390..1481
                     /label=Splicing Acceptor
                     /note="Splicing acceptor"
     CDS             1482..3772
                     /codon_start=1
                     /gene="NEO1"
                     /gene_synonym="IGDCC2; NGN; NTN1R2"
                     /product="neogenin isoform 1 precursor"
                     /label=NEO1
                     /note="isoform 1 precursor is encoded by transcriptvariant 
                     1; immunoglobulin superfamily DCC subclass member 2; 
                     neogenin homolog 1"
                     /db_xref="GI:157311649"
                     /db_xref="CCDS:CCDS10247.1"
                     /db_xref="GeneID:4756"
                     /db_xref="HGNC:HGNC:7754"
                     /db_xref="MIM:601907"
                     /protein_id="NP_002490.2"
                     /translation="LDRGTEYNFRVAALTINGTGPATDWLSAETFESDLDETRVPEVPS
                     SLHVRPLVTSIVVSWTPPENQNIVVRGYAIGYGIGSPHAQTIKVDYKQRYYTIENLDPS
                     SHYVITLKAFNNVGEGIPLYESAVTRPHTDTSEVDLFVINAPYTPVPDPTPMMPPVGVQ
                     ASILSHDTIRITWADNSLPKHQKITDSRYYTVRWKTNIPANTKYKNANATTLSYLVTGL
                     KPNTLYEFSVMVTKGRRSSTWSMTAHGTTFELVPTSPPKDVTVVSKEGKPKTIIVNWQP
                     PSEANGKITGYIIYYSTDVNAEIHDWVIEPVVGNRLTHQIQELTLDTPYYFKIQARNSK
                     GMGPMSEAVQFRTPKADSSDKMPNDQASGSGGKGSRLPDLGSDYKPPMSGSNSPHGSPT
                     SPLDSNMLLVIIVSVGVITIVVVVIIAVFCTRRTTSHQKKKRAACKSVNGSHKYKGNSK
                     DVKPPDLWIHHERLELKPIDKSPDPNPIMTDTPIPRNSQDITPVDNSMDSNIHQRRNSY
                     RGHESEDSMSTLAGRRGMRPKMMMPFDSQPPQPVISAHPIHSLDNPHHHFHSSSLASPA
                     RSHLYHPGSPWPIGTSMSLSDRANSTESVRNTPSTDTMPASSSQTCCTDHQDPEGATSS
                     SYLASSQEEDSGQSLPTAHVRPSHPLKSFAVPAIPPPGPPTYDPALPSTPLLSQQALNH
                     HIHSVKTASIGTLGRSRPPMPVVVPSAPEVQETTRMLEDSESSYEPDELTKEMAHLEGL
                     MKDLNAITTA"
     misc_feature    3617..3622
                     /label=SDS
     CDS             3779..3844
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     terminator      3848..4328
                     /label=W3SL
                     /note="Short WPRE and SV40 polyA"
     misc_feature    3992..3997
                     /label=SDS
     terminator      4150..4198
                     /label=W3SL
                     /note="Short WPRE and SV40 polyA"
     repeat_region   4371..4511
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     misc_feature    4371..4500
                     /label=AAV2 ITR
     repeat_region   4372..4512
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     rep_origin      4586..5041
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        5323..5427
                     /gene="bla"
                     /label=AmpR promoter
     CDS             5428..6288
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      6459..7047
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtcgctct gcgcgaggcg gctccgccgc ggcggaggga
      181 tacggcgcac catatatata tcgcggggcg cagactcgcg ctccggcagt ggtgctggga
      241 gtgtcgtgga cgccgtgccg ttactcgtac gccaccatgt ccaatttact gaccgtacac
      301 caaaatttgc ctgcattacc ggtcgatgca acgagtgatg aggttcgcaa gaacctgatg
      361 gacatgttca gggatcgcca ggcgttttct gagcatacct ggaaaatgct tctgtccgtt
      421 tgccggtcgt gggcggcatg gtgcaagttg aataaccgga aatggtttcc cgcagaacct
      481 gaagatgttc gcgattatct tctatatctt caggcgcgcg gtctggcagt aaaaaccatc
      541 cagcaacatt tgggccagct aaacatgctt catcgtcggt ccgggctgcc acgaccaagt
      601 gacagcaatg ctgtttcact ggttatgcgg cggatccgaa aagaaaacgt tgatgccggt
      661 gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt tcgaccaggt tcgttcactc
      721 atggaaaata gcgatcgctg ccaggatata cgtaatctgg catttctggg gattgcttat
      781 aacaccctgt tacgtatagc cgaaattgcc aggatcaggg ttaaagatat ctcacgtact
      841 gacggtggga gaatgttaat ccatattggc agaacgaaaa cgctggttag caccgcaggt
      901 gtagagaagg cacttagcct gggggtaact aaactggtcg agcgatggat ttccgtctct
      961 ggtgtagctg atgatccgaa taactacctg ttttgccggg tcagaaaaaa tggtgttgcc
     1021 gcgccatctg ccaccagcca gctatcaact cgcgccctgg aagggatttt tgaagcaact
     1081 catcgattga tttacggcgc taaggatgac tctggtcaga gatacctggc ctggtctgga
     1141 cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg ctggagtttc aataccggag
     1201 atcatgcaag ctggtggctg gaccaatgta aatattgtaa tgaactatat ccgtaacctg
     1261 gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg gcgattaggt aagtatcaag
     1321 gttacaagac aggtttaagg agaccaataa ctagtataac ttcgtatagg atactttata
     1381 gcaatttatg aaactgggct tgtcgagaca gagaagactc ttgcgtttct gataggcacc
     1441 tattggtctt actgacatcc actttgcctt tctctccaca ggtcttgatc gggggactga
     1501 gtataatttc cgagtggctg ctctaacaat caatggtaca ggcccggcaa ctgactggct
     1561 gtctgctgaa acttttgaaa gtgacctaga tgaaactcgt gttcctgaag tgcctagctc
     1621 tcttcacgta cgcccgctcg ttactagcat cgtagtgagc tggactcctc cagagaatca
     1681 gaacattgtg gtcagaggtt acgccattgg ttatggcatt ggcagccctc atgcccagac
     1741 catcaaagtg gactataaac agcgctatta caccattgaa aatctggatc ccagctctca
     1801 ctatgtgatt accctgaaag catttaataa cgtgggtgaa ggcatccccc tgtatgagag
     1861 tgctgtgacc aggcctcaca cagacacttc tgaagttgat ttatttgtta ttaatgctcc
     1921 atacactcca gtgccagatc ccactcccat gatgccacca gtgggagttc aggcttccat
     1981 tctgagtcat gacaccatca ggattacgtg ggcagacaac tcgctgccca agcaccagaa
     2041 gattacagac tcccgatact acaccgtccg atggaaaacc aacatcccag caaacaccaa
     2101 gtacaagaat gcaaatgcaa ccactttgag ttatttggtg actggtttaa agccgaatac
     2161 actctatgaa ttctctgtga tggtgaccaa aggtcgaaga tcaagtacat ggagtatgac
     2221 agcccatggg accacctttg aattagttcc gacttctcca cccaaggatg tgactgttgt
     2281 gagtaaagag gggaaaccta agaccataat tgtgaattgg cagcctccct ccgaagccaa
     2341 tggcaaaatt acaggttaca tcatatatta cagtacagat gtgaatgcag agatacatga
     2401 ctgggttatt gagcctgttg tgggaaacag actgactcac cagatacaag agttaactct
     2461 tgacacacca tactacttca aaatccaggc acggaactca aagggcatgg gacccatgtc
     2521 tgaagctgtc caattcagaa cacctaaagc ggactcctct gataaaatgc ctaatgatca
     2581 agcctcaggg tctggaggga aaggaagccg gctgccagac ctaggatccg actacaaacc
     2641 tccaatgagc ggcagtaaca gccctcatgg gagccccacc tctcctctgg acagtaatat
     2701 gctgctggtc ataattgttt ctgttggcgt catcaccatc gtggtggttg tgattatcgc
     2761 tgtcttttgt acccgtcgta ccacctctca ccagaaaaag aaacgagctg cctgcaaatc
     2821 agtgaatggc tctcataagt acaaagggaa ttccaaagat gtgaaacctc cagatctctg
     2881 gatccatcat gagagactgg agctgaaacc cattgataag tctccagacc caaaccccat
     2941 catgactgat actccaattc ctcgcaactc tcaagatatc acaccagttg acaactccat
     3001 ggacagcaat atccatcaaa ggcgaaattc atacagaggg catgagtcag aggacagcat
     3061 gtctacactg gctggaaggc gaggaatgag accaaaaatg atgatgccct ttgactccca
     3121 gccaccccag cctgtgatta gtgcccatcc catccattcc ctcgataacc ctcaccatca
     3181 tttccactcc agcagcctcg cttctccagc tcgcagtcat ctctaccacc cgggcagccc
     3241 atggcccatt ggcacatcca tgtccctttc agacagggcc aattccacag aatccgttcg
     3301 aaataccccc agcactgaca ccatgccagc ctcttcgtct caaacatgct gcactgatca
     3361 ccaggaccct gaaggtgcta ccagctcctc ttacttggcc agctcccaag aggaagattc
     3421 aggccagagt cttcccactg cccatgttcg cccttcccac ccattgaaga gcttcgccgt
     3481 gccagcaatc ccgcctccag gacctcccac ctatgatcct gcattgccaa gcacaccatt
     3541 actgtcccag caagctctga accatcacat tcactcagtg aagacagcct ccatcgggac
     3601 tctaggaagg agccggcctc ctatgccagt ggttgttccc agtgcccctg aagtgcagga
     3661 gaccacaagg atgttggaag actccgagag tagctatgaa ccagatgagc tgaccaaaga
     3721 gatggcccac ctggaaggac taatgaagga cctaaacgct atcacaacag catgtacaga
     3781 ctacaaagac catgacggtg attataaaga tcatgacatc gactacaagg atgacgatga
     3841 caagtaactc gagataatca acctctggat tacaaaattt gtgaaagatt gactggtatt
     3901 cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat
     3961 gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg gttagttctt
     4021 gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg
     4081 ggcactgaca attccgtggt gtttatttgt gaaatttgtg atgctattgc tttatttgta
     4141 accatctagc tttatttgtg aaatttgtga tgctattgct ttatttgtaa ccatctagct
     4201 ttatttgtga aatttgtgat gctattgctt tatttgtaac cattataagc tgcaataaac
     4261 aagttaacaa caacaattgc attcatttta tgtttcaggt tcagggggag atgtgggagg
     4321 ttttttaaag atctggagac cggtgtggca cgtgcggacc gagcggccgc aggaacccct
     4381 agtgatggag ttggccactc cctctctgcg cgctcgctcg ctcactgagg ccgggcgacc
     4441 aaaggtcgcc cgacgcccgg gctttgcccg ggcggcctca gtgagcgagc gagcgcgcag
     4501 ctgcctgcag gggcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca
     4561 ccgcatacgt caaagcaacc atagtacgcg ccctgtagcg gcgcattaag cgcggcgggt
     4621 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccttagcgcc cgctcctttc
     4681 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg
     4741 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat
     4801 ttgggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg
     4861 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaactct
     4921 atctcgggct attcttttga tttataaggg attttgccga tttcggtcta ttggttaaaa
     4981 aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac gtttacaatt
     5041 ttatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gccccgacac
     5101 ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga
     5161 caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa
     5221 cgcgcgagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata
     5281 atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
     5341 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
     5401 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
     5461 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
     5521 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc
     5581 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa
     5641 gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc
     5701 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt
     5761 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
     5821 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac
     5881 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata
     5941 ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta
     6001 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg
     6061 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat
     6121 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt
     6181 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga
     6241 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa
     6301 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
     6361 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
     6421 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     6481 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     6541 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     6601 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     6661 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     6721 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     6781 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     6841 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     6901 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     6961 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     7021 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     7081 gccttttgct ggccttttgc tcacatgt
//