| 质粒名称: | pAAVLINKv1-pCALM1-Cre-3'-NRXN2-FLAG |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001399 |
| 产品用途: | 3' AAVLINK plamid for NRXN2 expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒表达 |
| 启动子: | pCALM1 |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | NRXN2 |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
买家导航
pAAVLINKv1-pCALM1-Cre-3'-NRXN2-FLAG载体质粒基本信息
pAAVLINKv1-pCALM1-Cre-3'-NRXN2-FLAG质粒图谱载体图谱和pAAVLINKv1-pCALM1-Cre-3'-NRXN2-FLAG载体序列质粒序列多克隆位点信息
pAAVLINKv1-pCALM1-Cre-3'-NRXN2-FLAG质粒载体简介
pAAVLINKv1-pCALM1-Cre-3'-NRXN2-FLAG质粒序列载体序列
LOCUS pAAVLINKv1-pCALM1-Cre-3'-NRXN2-FLAG 6988 bp DNA circular SYN 11-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6988)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported May 6, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6988
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
promoter 145..265
/label=pCALM1
CDS 277..1308
/codon_start=1
/product="site-specific recombinase"
/label=Cre
/note="Cre is a site-specific recombinase from
bacteriophage P1. Recombination occurs at loxP sequences."
/translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
DGD"
intron 1309..1349
/label=Splicing Donor
protein_bind 1356..1389
/label=lox2272-JTZ17
/bound_moiety="Cre recombinase"
/note="Right element (RE) mutant of loxP (Araki et al.,
2010). Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
intron 1390..1481
/label=Splicing Acceptor
/note="Splicing acceptor"
CDS 1482..3701
/codon_start=1
/label=NRXN2
/translation="GYIHYVFDLGNGPSLMKGNSDKPVNDNQWHNVVVSRDPGNVHTLK
IDSRTVTQHSNGARNLDLKGELYIGGLSKNMFSNLPKLVASRDGFQGCLASVDLNGRLP
DLIADALHRIGQVERGCDGPSTTCTEESCANQGVCLQQWDGFTCDCTMTSYGGPVCNDP
GTTYIFGKGGALITYTWPPNDRPSTRMDRLAVGFSTHQRSAVLVRVDSASGLGDYLQLH
IDQGTVGVIFNVGTDDITIDEPNAIVSDGKYHVVRFTRSGGNATLQVDSWPVNERYPAG
NFDNERLAIARQRIPYRLGRVVDEWLLDKGRQLTIFNSQAAIKIGGRDQGRPFQGQVSG
LYYNGLKVLALAAESDPNVRTEGHLRLVGEGPSVLLSAETTATTLLADMATTIMETTTT
MATTTTRRGRSPTLRDSTTQNTDDLLVASAECPSDDEDLEECEPSTGGELILPIITEDS
LDPPPVATRSPFVPPPPTFYPFLTGVGATQDTLPPPAARRPPSGGPCQAERDDSDCEEP
IEASGFASGEVFDSSLPPTDDEDFYTTFPLVTDRTTLLSPRKPAPRPNLRTDGATGAPG
VLFAPSAPAPNLPAGKMNHRDPLQPLLENPPLGPGAPTSFEPRRPPPLRPGVTSAPGFP
HLPTANPTGPGERGPPGAVEVIRESSSTTGMVVGIVAAAALCILILLYAMYKYRNRDEG
SYQVDQSRNYISNSAQSNGAVVKEKAPAAPKTPSKAKKNKDKEYYV"
misc_feature 3140..3145
/label=SDS
misc_feature 3521..3526
/label=SDS
CDS 3708..3773
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
terminator 3777..4208
/label=W3SL
/note="Short WPRE and SV40 polyA"
misc_feature 3921..3926
/label=SDS
repeat_region 4251..4391
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 4251..4380
/label=AAV2 ITR
rep_origin 4466..4921
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 5203..5307
/gene="bla"
/label=AmpR promoter
CDS 5308..6168
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 6339..6927
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtcgctct gcgcgaggcg gctccgccgc ggcggaggga
181 tacggcgcac catatatata tcgcggggcg cagactcgcg ctccggcagt ggtgctggga
241 gtgtcgtgga cgccgtgccg ttactcgtac gccaccatgt ccaatttact gaccgtacac
301 caaaatttgc ctgcattacc ggtcgatgca acgagtgatg aggttcgcaa gaacctgatg
361 gacatgttca gggatcgcca ggcgttttct gagcatacct ggaaaatgct tctgtccgtt
421 tgccggtcgt gggcggcatg gtgcaagttg aataaccgga aatggtttcc cgcagaacct
481 gaagatgttc gcgattatct tctatatctt caggcgcgcg gtctggcagt aaaaaccatc
541 cagcaacatt tgggccagct aaacatgctt catcgtcggt ccgggctgcc acgaccaagt
601 gacagcaatg ctgtttcact ggttatgcgg cggatccgaa aagaaaacgt tgatgccggt
661 gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt tcgaccaggt tcgttcactc
721 atggaaaata gcgatcgctg ccaggatata cgtaatctgg catttctggg gattgcttat
781 aacaccctgt tacgtatagc cgaaattgcc aggatcaggg ttaaagatat ctcacgtact
841 gacggtggga gaatgttaat ccatattggc agaacgaaaa cgctggttag caccgcaggt
901 gtagagaagg cacttagcct gggggtaact aaactggtcg agcgatggat ttccgtctct
961 ggtgtagctg atgatccgaa taactacctg ttttgccggg tcagaaaaaa tggtgttgcc
1021 gcgccatctg ccaccagcca gctatcaact cgcgccctgg aagggatttt tgaagcaact
1081 catcgattga tttacggcgc taaggatgac tctggtcaga gatacctggc ctggtctgga
1141 cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg ctggagtttc aataccggag
1201 atcatgcaag ctggtggctg gaccaatgta aatattgtaa tgaactatat ccgtaacctg
1261 gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg gcgattaggt aagtatcaag
1321 gttacaagac aggtttaagg agaccaataa ctagtataac ttcgtatagg atactttata
1381 gcaatttatg aaactgggct tgtcgagaca gagaagactc ttgcgtttct gataggcacc
1441 tattggtctt actgacatcc actttgcctt tctctccaca ggggtacatc cactacgtgt
1501 ttgacctggg gaatggcccg tccttgatga aggggaactc agacaaacca gtcaatgaca
1561 accagtggca caacgtggtg gtgtccaggg acccaggcaa cgtgcacacg ctcaagattg
1621 actcccgcac tgtcacgcag cactccaatg gcgcccgaaa cctcgatctc aaaggggagt
1681 tgtacattgg cggtctgagc aagaatatgt tcagcaacct gcccaagctg gtggcctccc
1741 gggatggctt tcagggctgc ctggcctcag tggacctcaa cggacgtctc ccagacctca
1801 tcgccgacgc cctgcaccgc attgggcagg tggagagggg ctgtgatggc cccagcacca
1861 cctgcactga agagtcctgt gccaaccagg gcgtctgctt gcagcagtgg gatggcttca
1921 cctgcgactg caccatgact tcctatggag gccctgtctg caatgatccc gggaccacat
1981 acatctttgg gaagggggga gcgctcatca cctacacgtg gccccccaat gacaggccca
2041 gcacgaggat ggatcgcctg gccgtgggct tcagcaccca ccagcggagc gctgtgctgg
2101 tgcgggtgga cagcgcctcc ggccttggag actacctgca gctgcacatc gaccagggca
2161 ccgtgggggt gatctttaac gtgggcacgg acgacattac catcgacgag cccaacgcca
2221 tagtaagcga cggcaaatac cacgtggtgc gcttcactcg aagcggcggc aacgccaccc
2281 tgcaggtgga cagctggccg gtcaacgagc ggtacccggc aggaaacttt gataacgagc
2341 gcctggcgat tgctagacag agaatcccct accggcttgg tcgagtagta gatgagtggc
2401 tgctcgacaa aggccgccag ctgaccatct tcaacagcca ggctgccatc aagatcgggg
2461 gccgggatca gggccgcccc ttccagggcc aggtgtccgg cctctactac aatgggctca
2521 aggtgctggc gctggccgcc gagagcgacc ccaatgtgcg gactgagggt cacctgcgcc
2581 tggtggggga ggggccgtcc gtgctgctca gtgcggagac cacggccacc accctgctgg
2641 ctgacatggc caccaccatc atggagacta ccaccaccat ggccactacc accacgcgcc
2701 ggggccgctc ccccacactg agggacagca ccacccagaa cacagatgac ctgctggtgg
2761 cctctgctga gtgtccaagc gatgatgagg acctggagga gtgtgagccc agtactggag
2821 gagagttaat attgcccatt atcacggagg actccttaga cccccctccc gtggccaccc
2881 gatccccctt cgtgcccccg ccccctacct tctacccctt cctcacggga gtgggcgcca
2941 cccaagacac gctgcccccg cccgccgcgc gccgcccgcc ctctgggggc ccgtgccagg
3001 ccgagcggga cgacagcgac tgcgaggagc ccatcgaggc ctcgggcttc gcctccgggg
3061 aggtctttga ctccagcctc ccccccacgg acgacgagga cttttacacc acctttcccc
3121 tggtcacgga ccgcaccacc ctcctgtcac cccgcaaacc cgctccccgg cccaacctca
3181 ggacagatgg ggccacgggc gcccctgggg tgctgtttgc cccctccgcc ccggccccca
3241 acctgccggc gggcaaaatg aaccaccgag acccgcttca gcccttgctg gagaacccgc
3301 ccttggggcc cggggccccc acgtcctttg agccgcggag gccccctccc ctgcgccccg
3361 gcgtgacctc agcccccggc ttcccccatc tgcccacagc caaccccaca gggcctgggg
3421 agcggggccc gccgggcgca gtggaggtga tccgggagtc cagcagcacc acgggcatgg
3481 tggtgggcat tgtggcggcg gcggcgctct gcatcctcat cctcctctac gccatgtata
3541 agtaccgcaa tcgtgatgag ggctcctacc aggtggacca gagccgaaac tacatcagta
3601 actcggccca gagcaatggg gcggtggtga aagagaaggc cccggctgcc cccaagacgc
3661 ccagcaaggc caagaagaac aaagacaagg agtattatgt ctgtacagac tacaaagacc
3721 atgacggtga ttataaagat catgacatcg actacaagga tgacgatgac aagtaactcg
3781 agataatcaa cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt
3841 tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc
3901 ccgtatggct ttcattttct cctccttgta taaatcctgg ttagttcttg ccacggcgga
3961 actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa
4021 ttccgtggtg tttatttgtg aaatttgtga tgctattgct ttatttgtaa ccatctagct
4081 ttatttgtga aatttgtgat gctattgctt tatttgtaac cattataagc tgcaataaac
4141 aagttaacaa caacaattgc attcatttta tgtttcaggt tcagggggag atgtgggagg
4201 ttttttaaag atctggagac cggtgtggca cgtgcggacc gagcggccgc aggaacccct
4261 agtgatggag ttggccactc cctctctgcg cgctcgctcg ctcactgagg ccgggcgacc
4321 aaaggtcgcc cgacgcccgg gctttgcccg ggcggcctca gtgagcgagc gagcgcgcag
4381 ctgcctgcag gggcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca
4441 ccgcatacgt caaagcaacc atagtacgcg ccctgtagcg gcgcattaag cgcggcgggt
4501 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccttagcgcc cgctcctttc
4561 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg
4621 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat
4681 ttgggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg
4741 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaactct
4801 atctcgggct attcttttga tttataaggg attttgccga tttcggtcta ttggttaaaa
4861 aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac gtttacaatt
4921 ttatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gccccgacac
4981 ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga
5041 caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa
5101 cgcgcgagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata
5161 atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
5221 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
5281 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
5341 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
5401 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc
5461 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa
5521 gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc
5581 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt
5641 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
5701 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac
5761 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata
5821 ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta
5881 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg
5941 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat
6001 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt
6061 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga
6121 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa
6181 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
6241 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
6301 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
6361 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
6421 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
6481 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
6541 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
6601 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
6661 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
6721 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
6781 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
6841 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
6901 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
6961 gccttttgct ggccttttgc tcacatgt
//
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