pDEST-FRT/T0-Flag-BRCA1

LOCUS       pDEST-FRT/T0-Flag-BRCA1               10795 bp ds-DNA     circular SYN 22-JAN-2019
DEFINITION  mammalian expession of Flag-BRCA1.
ACCESSION   .
VERSION     .
KEYWORDS    pDEST-FRT/T0-Flag-BRCA1
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10795)
  AUTHORS   Orthwein A, Noordermeer SM, Wilson MD, Landry S, Enchev RI, Sherker 
            A, Munro M, Pinder J, Salsman J, Dellaire G, Xia B, Peter M, 
            Durocher D
  TITLE     A mechanism for the suppression of homologous recombination in G1 
            cells.
  JOURNAL   Nature. 2015 Dec 9. doi: 10.1038/nature16142.
  PUBMED    26649820
REFERENCE   2  (bases 1 to 10795)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 22, 2019 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10795
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    820..838
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    841..859
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     869..893
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             1034..1057
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    1058..1082
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(6681..6705)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(6747..6764)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6753..6977
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(7110..7129)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    7261..7308
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             7316..8338
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGDFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    8468..8589
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8505..8524)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8559..8578
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8638..8654)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8638..8654)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8651..8673)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8662..8678
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(8686..8716)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8731..8752
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8869..8886)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9040..9628)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9120..9139)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(9799..10659)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10422..10441
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10660..10764)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ccctatcagt gatagagatc
      841 tccctatcag tgatagagat cgtcgacgag ctcgtttagt gaaccgtcag atcgcctgga
      901 gacgccatcc acgctgtttt gacctccata gaagacaccg ggaccgatcc agcctccgga
      961 ctctagcgtt taaacttaag cttccaccat ggactacaaa gaccatgacg gtgatacata
     1021 tgatcatgac atcgattaca aggatgacga tgacaagaca agtttgtaca aaaaagcagg
     1081 cttcaccatg gatttatctg ctcttcgcgt tgaagaagta caaaatgtca ttaatgctat
     1141 gcagaaaatc ttagagtgtc ccatctgtct ggagttgatc aaggaacctg tctccacaaa
     1201 gtgtgaccac atattttgca aattttgcat gctgaaactt ctcaaccaga agaaagggcc
     1261 ttcacagtgt cctttatgta agaatgatat aaccaaaagg agcctacaag aaagtacgag
     1321 atttagtcaa cttgttgaag agctattgaa aatcatttgt gcttttcagc ttgacacagg
     1381 tttggagtat gcaaacagct ataattttgc aaaaaaggaa aataactctc ctgaacatct
     1441 aaaagatgaa gtttctatca tccaaagtat gggctacaga aaccgtgcca aaagacttct
     1501 acagagtgaa cccgaaaatc cttccttgca ggaaaccagt ctcagtgtcc aactctctaa
     1561 ccttggaact gtgagaactc tgaggacaaa gcagcggata caacctcaaa agacgtctgt
     1621 ctacattgaa ttgggatctg attcttctga agataccgtt aataaggcaa cttattgcag
     1681 tgtgggagat caagaattgt tacaaatcac ccctcaagga accagggatg aaatcagttt
     1741 ggattctgca aaaaaggctg cttgtgaatt ttctgagacg gatgtaacaa atactgaaca
     1801 tcatcaaccc agtaataatg atttgaacac cactgagaag cgtgcagctg agaggcatcc
     1861 agaaaagtat cagggtagtt ctgtttcaaa cttgcatgtg gagccatgtg gcacaaatac
     1921 tcatgccagc tcattacagc atgagaacag cagtttatta ctcactaaag acagaatgaa
     1981 tgtagaaaag gctgaattct gtaataaaag caaacagcct ggcttagcaa ggagccaaca
     2041 taacagatgg gctggaagta aggaaacatg taatgatagg cggactccca gcacagaaaa
     2101 aaaggtagat ctgaatgctg atcccctgtg tgagagaaaa gaatggaata agcagaaact
     2161 gccatgctca gagaatccta gagatactga agatgttcct tggataacac taaatagcag
     2221 cattcagaaa gttaatgagt ggttttccag aagtgatgaa ctgttaggtt ctgatgactc
     2281 acatgatggg gagtctgaat caaatgccaa agtagctgat gtattggacg ttctaaatga
     2341 ggtagatgaa tattctggtt cttcagagaa aatagactta ctggccagtg atcctcatga
     2401 ggctttaata tgtaaaagtg aaagagttca ctccaaatca gtagagagta atattgaaga
     2461 caaaatattt gggaaaacct atcggaagaa ggcaagcctc cccaacttaa gccatgtaac
     2521 tgaaaatcta attataggag catttgttac tgagccacag ataatacaag agcgtcccct
     2581 cacaaataaa ttaaagcgta aaaggagacc tacatcaggc cttcatcctg aggattttat
     2641 caagaaagca gatttggcag ttcaaaagac tcctgaaatg ataaatcagg gaactaacca
     2701 aacggagcag aatggtcaag tgatgaatat tactaatagt ggtcatgaga ataaaacaaa
     2761 aggtgattct attcagaatg agaaaaatcc taacccaata gaatcactcg aaaaagaatc
     2821 tgctttcaaa acgaaagctg aacctataag cagcagtata agcaatatgg aactcgaatt
     2881 aaatatccac aattcaaaag cacctaaaaa gaataggctg aggaggaagt cttctaccag
     2941 gcatattcat gcgcttgaac tagtagtcag tagaaatcta agcccaccta attgtactga
     3001 attgcaaatt gatagttgtt ctagcagtga agagataaag aaaaaaaagt acaaccaaat
     3061 gccagtcagg cacagcagaa acctacaact catggaaggt aaagaacctg caactggagc
     3121 caagaagagt aacaagccaa atgaacagac aagtaaaaga catgacagtg atactttccc
     3181 agagctgaag ttaacaaatg cacctggttc ttttactaag tgttcaaata ccagtgaact
     3241 taaagaattt gtcaatccta gccttccaag agaagaaaaa gaagagaaac tagaaacagt
     3301 taaagtgtct aataatgctg aagaccccaa agatctcatg ttaagtggag aaagggtttt
     3361 gcaaactgaa agatctgtag agagtagcag tatttcactg gtacctggta ctgattatgg
     3421 cactcaggaa agtatctcgt tactggaagt tagcactcta gggaaggcaa aaacagaacc
     3481 aaataaatgt gtgagtcagt gtgcagcatt tgaaaacccc aagggactaa ttcatggttg
     3541 ttccaaagat aatagaaatg acacagaagg ctttaagtat ccattgggac atgaagttaa
     3601 ccacagtcgg gaaacaagca tagaaatgga agaaagtgaa cttgatgctc agtatttgca
     3661 gaatacattc aaggtttcaa agcgccagtc atttgctctg ttttcaaatc caggaaatgc
     3721 agaagaggaa tgtgcaacat tctctgccca ctctgggtcc ttaaagaaac aaagtccaaa
     3781 agtcactttt gaatgtgaac aaaaggaaga aaatcaagga aagaatgagt ctaatatcaa
     3841 gcctgtacag acagttaata tcactgcagg ctttcctgtg gttggtcaga aagataagcc
     3901 agttgataat gccaaatgta gtatcaaagg aggctctagg ttttgtctat catctcagtt
     3961 cagaggcaac gaaactggac tcattactcc aaataaacat ggacttttac aaaacccata
     4021 tcgtatacca ccactttttc ccatcaagtc atttgttaaa actaaatgta agaaaaatct
     4081 gctagaggaa aactttgagg aacattcaat gtcacctgaa agagaaatgg gaaatgagaa
     4141 cattccaagt acagtgagca caattagccg taataacatt agagaaaatg tttttaaagg
     4201 agccagctca agcaatatta atgaagtagg ttccagtact aatgaagtgg gctccagtat
     4261 taatgaaata ggttccagtg atgaaaacat tcaagcagaa ctaggtagaa acagagggcc
     4321 aaaattgaat gctatgctta gattaggggt tttgcaacct gaggtctata aacaaagtct
     4381 tcctggaagt aattgtaagc atcctgaaat aaaaaagcaa gaatatgaag aagtagttca
     4441 gactgttaat acagatttct ctccatatct gatttcagat aacttagaac agcctatggg
     4501 aagtagtcat acatctcagg tttgttctga gacacctgat gacctgttag atgatggtga
     4561 aataaaggaa gatactagtt ttgctgaaaa tgacattaag gaaagttctg ctgtttttag
     4621 caaaagcgtc cagagaggag agcttagcag gagtcctagc cctttcaccc atacacattt
     4681 ggctcagggt taccgaagag gggccaagaa attagagtcc tcagaagaga acttatctag
     4741 tgaggatgaa gagcttccct gcttccaaca cttgttattt ggtaaagtaa acaatatacc
     4801 ttctcagtct actaggcata gcaccgttgc taccgagtgt ctgtctaaga acacagagga
     4861 gaatttatta tcattgaaga atagcttaaa tgactgcagt aaccaggtaa tattggcaaa
     4921 ggcatctcag gaacatcacc ttagtgagga aacaaaatgt tctgctagct tgttttcttc
     4981 acagtgcagt gaattggaag acttgactgc aaatacaaac acccaggatc ctttcttgat
     5041 tggttcttcc aaacaaatga ggcatcagtc tgaaagccag ggagttggtc tgagtgacaa
     5101 ggaattggtt tcagatgatg aagaaagagg aacgggcttg gaagaaaata atcaagaaga
     5161 gcaaagcatg gattcaaact taggtgaagc agcatctggg tgtgagagtg aaacaagcgt
     5221 ctctgaagac tgctcagggc tatcctctca gagtgacatt ttaaccactc agcagaggga
     5281 taccatgcaa cataacctga taaagctcca gcaggaaatg gctgaactag aagctgtgtt
     5341 agaacagcat gggagccagc cttctaacag ctaccctagt ataatcagtg actcttctgc
     5401 ccttgaggac ctgcgaaatc cagaacaaag cacatcagaa aaagcagtat taacttcaca
     5461 gaaaagtagt gaatacccta taagccagaa tccagaaggc ctttctgctg acaagtttga
     5521 ggtgtctgca gatagttcta ccagtaaaaa taaagaacca ggagtggaaa ggtcatcccc
     5581 ttctaaatgc ccatcattag atgataggtg gtacatgcac agttgctctg ggagtcttca
     5641 gaatagaaac tacccatctc aagaggagct cattaaggtt gttgatgtgg aggagcaaca
     5701 gctggaagag tctgggccac acgatttgac ggaaacatct tacttgccaa ggcaagatct
     5761 agagggaacc ccttacctgg aatctggaat cagcctcttc tctgatgacc ctgaatctga
     5821 tccttctgaa gacagagccc cagagtcagc tcgtgttggc aacataccat cttcaacctc
     5881 tgcattgaaa gttccccaat tgaaagttgc agaatctgcc cagggtccag ctgctgctca
     5941 tactactgat actgctgggt ataatgcaat ggaagaaagt gtgagcaggg agaagccaga
     6001 attgacagct tcaacagaaa gggtcaacaa aagaatgtcc atggtggtgt ctggcctgac
     6061 cccagaagaa tttatgctcg tgtacaagtt tgccagaaaa caccacatca ctttaactaa
     6121 tctaattact gaagagacta ctcatgttgt tatgaaaaca gatgctgagt ttgtgtgtga
     6181 acggacactg aaatattttc taggaattgc gggaggaaaa tgggtagtta gctatttctg
     6241 ggtgacccag tctattaaag aaagaaaaat gctgaatgag catgattttg aagtcagagg
     6301 agatgtggtc aatggaagaa accaccaagg tccaaagcga gcaagagaat cccaggacag
     6361 aaagatcttc agggggctag aaatctgttg ctatgggccc ttcaccaaca tgcccacaga
     6421 tcaactggaa tggatggtac agctgtgtgg tgcttctgtg gtgaaggagc tttcatcatt
     6481 cacccttggc acaggtgtcc acccaattgt ggttgtgcag ccagatgcct ggacagagga
     6541 caatggcttc catgcaattg ggcagatgtg tgaggcacct gtggtgaccc gagagtgggt
     6601 gttggacagt gtagcactct accagtgcca ggagctggac acctacctga taccccagat
     6661 cccccacagc cactactgag acccagcttt cttgtacaaa gtggtctgac tcgagtctag
     6721 agggcccgtt taaacccgct gatcagcctc gactgtgcct tctagttgcc agccatctgt
     6781 tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc
     6841 ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg
     6901 tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga
     6961 tgcggtgggc tctatggctt ctgaggcgga aagaaccagc tggggctcta gggggtatcc
     7021 ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac
     7081 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc
     7141 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt
     7201 tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtaccta
     7261 gaagttccta ttccgaagtt cctattctct agaaagtata ggaacttcct tggccaaaaa
     7321 gcctgaactc accgcgacgt ctgtcgagaa gtttctgatc gaaaagttcg acagcgtctc
     7381 cgacctgatg cagctctcgg agggcgaaga atctcgtgct ttcagcttcg atgtaggagg
     7441 gcgtggatat gtcctgcggg taaatagctg cgccgatggt ttctacaaag atcgttatgt
     7501 ttatcggcac tttgcatcgg ccgcgctccc gattccggaa gtgcttgaca ttggggattt
     7561 cagcgagagc ctgacctatt gcatctcccg ccgtgcacag ggtgtcacgt tgcaagacct
     7621 gcctgaaacc gaactgcccg ctgttctgca gccggtcgcg gaggccatgg atgcgatcgc
     7681 tgcggccgat cttagccaga cgagcgggtt cggcccattc ggaccgcaag gaatcggtca
     7741 atacactaca tggcgtgatt tcatatgcgc gattgctgat ccccatgtgt atcactggca
     7801 aactgtgatg gacgacaccg tcagtgcgtc cgtcgcgcag gctctcgatg agctgatgct
     7861 ttgggccgag gactgccccg aagtccggca cctcgtgcac gcggatttcg gctccaacaa
     7921 tgtcctgacg gacaatggcc gcataacagc ggtcattgac tggagcgagg cgatgttcgg
     7981 ggattcccaa tacgaggtcg ccaacatctt cttctggagg ccgtggttgg cttgtatgga
     8041 gcagcagacg cgctacttcg agcggaggca tccggagctt gcaggatcgc cgcggctccg
     8101 ggcgtatatg ctccgcattg gtcttgacca actctatcag agcttggttg acggcaattt
     8161 cgatgatgca gcttgggcgc agggtcgatg cgacgcaatc gtccgatccg gagccgggac
     8221 tgtcgggcgt acacaaatcg cccgcagaag cgcggccgtc tggaccgatg gctgtgtaga
     8281 agtactcgcc gatagtggaa accgacgccc cagcactcgt ccgagggcaa aggaatagca
     8341 cgtactacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt
     8401 tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc
     8461 ccaccccaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
     8521 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
     8581 tgtatcttat catgtctgta taccgtcgac ctctagctag agcttggcgt aatcatggtc
     8641 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg
     8701 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt
     8761 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg
     8821 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
     8881 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
     8941 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
     9001 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     9061 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     9121 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     9181 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     9241 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     9301 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     9361 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     9421 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     9481 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     9541 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     9601 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     9661 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     9721 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     9781 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
     9841 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
     9901 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
     9961 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
    10021 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
    10081 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
    10141 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
    10201 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
    10261 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
    10321 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
    10381 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
    10441 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
    10501 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
    10561 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
    10621 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
    10681 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
    10741 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtc
//