pAAVLINKv2-SCP1-Cre-UDeg3a-3'-MYO5A-FLAG

LOCUS       pAAVLINKv2-SCP1-Cre-UDeg3a-3'-MYO5A-FLAG              6676 bp DNA     circular SYN 25-NOV-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6676)
  AUTHORS   Stavrini Papadaki
  TITLE     Direct Submission
  JOURNAL   Exported Jun 11, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..6676
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
                     /note="color: #ffe4c4"
     misc_feature    145..225
                     /label=SCP1
     CDS             237..1265
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
                     LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
                     PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
                     LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
                     RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
                     RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
                     DGD"
     CDS             1281..1370
                     /codon_start=1
                     /label=UDeg3a
                     /translation="LLLKLLLLGGGSGPRPRGTRGKGRRIHYKG"
     misc_feature    1295..1300
                     /label=SDS
     misc_feature    1298..1303
                     /label=SDS
     intron          1374..1414
                     /label=Splicing Donor
     protein_bind    1421..1454
                     /label=lox2272-JTZ17
                     /bound_moiety="Cre recombinase"
                     /note="Right element (RE) mutant of loxP (Araki et al., 
                     2010). Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     intron          1455..1546
                     /label=Splicing Acceptor
                     /note="Splicing acceptor"
     CDS             1547..3377
                     /codon_start=1
                     /gene="MYO5A"
                     /gene_synonym="GS1; MYH12; MYO5; MYR12"
                     /product="unconventional myosin-Va isoform 3"
                     /label=MYO5A
                     /note="isoform 3 is encoded by transcript variant 
                     3;myosin-Va; myoxin; myosin V; myosin, heavy polypeptide 
                     kinase; myosin-12; dilute myosin heavy chain, non-muscle; 
                     unconventional myosin-Va; myosin VA (heavy chain 12, 
                     myoxin)"
                     /db_xref="GI:1835953126"
                     /db_xref="CCDS:CCDS92001.1"
                     /db_xref="GeneID:4644"
                     /db_xref="HGNC:HGNC:7602"
                     /db_xref="MIM:160777"
                     /protein_id="NP_001369276.1"
                     /translation="SQLVSQKEAIQPKDDKNTMTDSTILLEDVQKMKDKGEIAQAYIGL
                     KETNRSSALDYHELNEDGELWLVYEGLKQANRLLESQLQSQKRSHENEAEALRGEIQSL
                     KEENNRQQQLLAQNLQLPPEARIEASLQHEITRLTNENLYFEELYADDPKKYQSYRISL
                     YKRMIDLMEQLEKQDKTVRKLKKQLKVFAKKIGELEVGQMENISPGQIIDEPIRPVNIP
                     RKEKDFQGMLEYKKEDEQKLVKNLILELKPRGVAVNLIPGLPAYILFMCVRHADYLNDD
                     QKVRSLLTSTINSIKKVLKKRGDDFETVSFWLSNTCRFLHCLKQYSGEEGFMKHNTSRQ
                     NEHCLTNFDLAEYRQVLSDLAIQIYQQLVRVLENILQPMIVSGMLEHETIQGVSGVKPT
                     GLRKRTSSIADEGTYTLDSILRQLNSFHSVMCQHGMDPELIKQVVKQMFYIIGAITLNN
                     LLLRKDMCSWSKGMQIRYNVSQLEEWLRDKNLMNSGAKETLEPLIQAAQLLQVKKKTDD
                     DAEAICSMCNALTTAQIVKVLNLYTPVNEFEERVSVSFIRTIQMRLRDRKDSPQLLMDA
                     KHIFPVTFPFNPSSLALETIQIPASLGLGFISRV"
     misc_feature    1861..1866
                     /label=SDS
     misc_feature    2254..2259
                     /label=SDS
     CDS             3384..3449
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     terminator      3465..3896
                     /label=W3SL
                     /note="Short WPRE and SV40 polyA"
     misc_feature    3609..3614
                     /label=SDS
     repeat_region   3939..4079
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     misc_feature    3939..4068
                     /label=AAV2 ITR
     repeat_region   3940..4080
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     rep_origin      4154..4609
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        4891..4995
                     /gene="bla"
                     /label=AmpR promoter
     CDS             4996..5856
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      6027..6615
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtgtactt atataagggg gtgggggcgc gttcgtcctc
      181 agtcgcgatc gaacactcga gccgagcaga cgtgcctacg gaccgcgtac gccaccatgt
      241 ccaatttact gaccgtacac caaaatttgc ctgcattacc ggtcgatgca acgagtgatg
      301 aggttcgcaa gaacctgatg gacatgttca gggatcgcca ggcgttttct gagcatacct
      361 ggaaaatgct tctgtccgtt tgccggtcgt gggcggcatg gtgcaagttg aataaccgga
      421 aatggtttcc cgcagaacct gaagatgttc gcgattatct tctatatctt caggcgcgcg
      481 gtctggcagt aaaaaccatc cagcaacatt tgggccagct aaacatgctt catcgtcggt
      541 ccgggctgcc acgaccaagt gacagcaatg ctgtttcact ggttatgcgg cggatccgaa
      601 aagaaaacgt tgatgccggt gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt
      661 tcgaccaggt tcgttcactc atggaaaata gcgatcgctg ccaggatata cgtaatctgg
      721 catttctggg gattgcttat aacaccctgt tacgtatagc cgaaattgcc aggatcaggg
      781 ttaaagatat ctcacgtact gacggtggga gaatgttaat ccatattggc agaacgaaaa
      841 cgctggttag caccgcaggt gtagagaagg cacttagcct gggggtaact aaactggtcg
      901 agcgatggat ttccgtctct ggtgtagctg atgatccgaa taactacctg ttttgccggg
      961 tcagaaaaaa tggtgttgcc gcgccatctg ccaccagcca gctatcaact cgcgccctgg
     1021 aagggatttt tgaagcaact catcgattga tttacggcgc taaggatgac tctggtcaga
     1081 gatacctggc ctggtctgga cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg
     1141 ctggagtttc aataccggag atcatgcaag ctggtggctg gaccaatgta aatattgtaa
     1201 tgaactatat ccgtaacctg gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg
     1261 gcgatggagg tggatctggt ctgctgctga aactcctcct cctcggtggt ggttctggtc
     1321 ctcgtccccg tggtactcgt ggtaaaggtc gccgtattca ctacaaagga taagtaagta
     1381 tcaaggttac aagacaggtt taaggagacc aataactagt ataacttcgt ataggatact
     1441 ttatagcaat ttatgaaact gggcttgtcg agacagagaa gactcttgcg tttctgatag
     1501 gcacctattg gtcttactga catccacttt gcctttctct ccacaggtct caactggtga
     1561 gccagaaaga ggccatccaa cccaaggatg acaagaatac aatgacagat tccacaatac
     1621 ttttggaaga tgtacaaaaa atgaaagata aaggtgaaat agcacaagca tacattggtt
     1681 tgaaagaaac aaatagatca tctgctctgg attaccatga gttgaatgag gatggagagc
     1741 tgtggctggt ttatgaaggg ttaaaacaag ccaacaggct cctggaatcc cagctgcagt
     1801 cacagaagag gagccatgag aatgaggccg aggccctccg tggggagatc cagagcctga
     1861 aggaggagaa caaccgacag cagcagctgc tggcccagaa cctgcagctg cccccagagg
     1921 cccgcattga ggccagcctg cagcacgaga tcacccggct gaccaacgaa aacttgtatt
     1981 ttgaggaatt atatgcagat gaccctaaga agtatcaatc atatcggatt tccctttaca
     2041 aacggatgat tgatttgatg gaacaacttg aaaaacagga taagacggtc cgtaaactga
     2101 aaaaacaact gaaagtattt gccaaaaaaa ttggcgaact agaagtgggc cagatggaga
     2161 acatatcccc aggacagatc attgatgaac ccatccgacc agtcaacatt cccaggaaag
     2221 aaaaggattt ccaagggatg ctggaataca agaaggagga tgagcaaaaa cttgttaaga
     2281 acctgattct ggaactgaag ccacgtggtg tagcagtcaa tttgattcca ggattaccgg
     2341 catatatcct gttcatgtgt gttcgacatg ctgactacct gaatgatgat cagaaagtaa
     2401 ggtcgttgct aacatcaaca attaacagca tcaaaaaagt attgaagaaa agaggtgatg
     2461 attttgaaac cgtctccttc tggctctcta acacatgccg atttttgcac tgcttgaaac
     2521 agtacagtgg agaagagggc tttatgaagc acaacacatc tcgccagaat gaacactgcc
     2581 tcaccaattt tgacctggct gagtatcggc aggtgctgag tgacttggcc attcagatct
     2641 accagcagct cgtgcgggtg ttagagaaca tccttcagcc aatgattgtc tcaggcatgc
     2701 tggaacatga aacgattcag ggcgtgtctg gggtgaagcc cacagggttg agaaagcgaa
     2761 cctccagtat cgccgatgag ggcacctaca cactggactc catcctccgg cagctcaact
     2821 ccttccactc ggtcatgtgt cagcatggca tggaccctga actgatcaag caggtggtca
     2881 agcagatgtt ctacatcata ggggccatca ccctgaacaa ccttctcctg cggaaggaca
     2941 tgtgctcctg gagtaaaggc atgcagatca ggtacaatgt cagtcaactg gaagaatggc
     3001 tgcgtgacaa gaatctgatg aatagtgggg ctaaagaaac cctggaacct ctcattcagg
     3061 ctgctcaact tttgcaagtg aaaaagaaaa cagatgatga tgcagaagcc atttgttcta
     3121 tgtgcaatgc tttaactact gcccagattg tgaaagtgtt gaatttgtat actccagtta
     3181 atgagtttga agaaagagtc tctgtgtcgt tcattcgtac tatacagatg cgtttacgag
     3241 acaggaaaga ctctccccag ctgctcatgg atgctaaaca catctttcct gtcacctttc
     3301 ctttcaaccc atcttccctc gcactagaaa ccatccagat tccagccagc ctcggcctgg
     3361 gcttcatttc acgggtctgt acagactaca aagaccatga cggtgattat aaagatcatg
     3421 acatcgacta caaggatgac gatgacaagt aagaattctg atgactcgag ataatcaacc
     3481 tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac
     3541 gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt
     3601 cattttctcc tccttgtata aatcctggtt agttcttgcc acggcggaac tcatcgccgc
     3661 ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt
     3721 tatttgtgaa atttgtgatg ctattgcttt atttgtaacc atctagcttt atttgtgaaa
     3781 tttgtgatgc tattgcttta tttgtaacca ttataagctg caataaacaa gttaacaaca
     3841 acaattgcat tcattttatg tttcaggttc agggggagat gtgggaggtt ttttaaagat
     3901 ctggagaccg gtgtggcacg tgcggaccga gcggccgcag gaacccctag tgatggagtt
     3961 ggccactccc tctctgcgcg ctcgctcgct cactgaggcc gggcgaccaa aggtcgcccg
     4021 acgcccgggc tttgcccggg cggcctcagt gagcgagcga gcgcgcagct gcctgcaggg
     4081 gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatacgtca
     4141 aagcaaccat agtacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
     4201 cgcagcgtga ccgctacact tgccagcgcc ttagcgcccg ctcctttcgc tttcttccct
     4261 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta
     4321 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgattt gggtgatggt
     4381 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg
     4441 ttctttaata gtggactctt gttccaaact ggaacaacac tcaactctat ctcgggctat
     4501 tcttttgatt tataagggat tttgccgatt tcggtctatt ggttaaaaaa tgagctgatt
     4561 taacaaaaat ttaacgcgaa ttttaacaaa atattaacgt ttacaatttt atggtgcact
     4621 ctcagtacaa tctgctctga tgccgcatag ttaagccagc cccgacaccc gccaacaccc
     4681 gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc
     4741 gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgagacga
     4801 aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag
     4861 acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
     4921 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
     4981 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
     5041 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
     5101 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
     5161 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
     5221 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat
     5281 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
     5341 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
     5401 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
     5461 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
     5521 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa
     5581 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
     5641 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
     5701 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
     5761 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
     5821 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
     5881 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
     5941 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     6001 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     6061 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     6121 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
     6181 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     6241 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     6301 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     6361 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     6421 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     6481 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     6541 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     6601 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     6661 ccttttgctc acatgt
//