pSL1468

LOCUS       pSL1468            7484 bp DNA     circular SYN 30-MAR-2026
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7484)
  AUTHORS   Trial User
  TITLE     Direct Submission
  JOURNAL   Exported Aug 30, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..7484
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     primer_bind     123..164
                     /label=oSCL1800
     misc_feature    143..725
                     /label=CMV
     enhancer        146..525
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        526..729
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(699..730)
                     /label=OXB018
     primer_bind     706..749
                     /label=oSCL1791
     primer_bind     709..729
                     /label=oSCL1013
     misc_feature    731..732
                     /label=linker
     primer_bind     complement(734..748)
                     /label=Oscl28-2
     primer_bind     complement(735..748)
                     /label=8x R
     misc_feature    749..761
                     /label=linker
     misc_feature    767..785
                     /label=MS2 box
     misc_feature    791..800
                     /label=linker
     misc_feature    806..824
                     /label=MS2 box
     misc_feature    830..839
                     /label=linker
     misc_feature    830..839
                     /label=linker
     misc_feature    838..868
                     /label=hairpin
     misc_feature    844..862
                     /label=MS2 box
     misc_feature    867..879
                     /label=linker
     misc_feature    878..908
                     /label=hairpin
     misc_feature    884..902
                     /label=MS2 box
     misc_feature    912..917
                     /label=linker
     primer_bind     917..943
                     /label=oSCL2462
     misc_feature    926..941
                     /label=linker
     misc_feature    968..980
                     /label=linker
     misc_feature    986..1004
                     /label=MS2 box
     misc_feature    1010..1019
                     /label=linker
     misc_feature    1025..1043
                     /label=MS2 box
     misc_feature    1049..1058
                     /label=linker
     misc_feature    1049..1058
                     /label=linker
     misc_feature    1057..1087
                     /label=hairpin
     misc_feature    1063..1081
                     /label=MS2 box
     misc_feature    1086..1098
                     /label=linker
     misc_feature    1097..1127
                     /label=hairpin
     misc_feature    1103..1121
                     /label=MS2 box
     misc_feature    1131..1136
                     /label=linker
     primer_bind     1136..1187
                     /label=oSCL2462
     misc_feature    1145..1160
                     /label=linker
     primer_bind     1167..1187
                     /label=Oscl31-3
     misc_feature    1167..1170
                     /label=New Feature
     misc_feature    1167..1170
                     /label=New Feature
     misc_feature    1170
                     /label=EGFP
                     /note="note=""Fragment extracted from OWS233-Kpn1-EGFP-Xba1
                     [758  nt] : (KpnI[4] / XbaI[766])"
     primer_bind     1173..1187
                     /label=oSCL775
     primer_bind     1175..1187
                     /label=oSCL456
     misc_feature    1181..2740
                     /label=SEAP
     CDS             1181..2683
                     /codon_start=1
                     /product="secreted alkaline phosphatase from human 
                     placenta"
                     /label=SEAP
                     /note="The natural enzyme has a C-terminal transmembrane 
                     sequence that is replaced by a GPI anchor."
                     /translation="MLLLLLLLGLRLQLSLGIIPVEEENPDFWNREAAEALGAAKKLQP
                     AQTAAKNLIIFLGDGMGVSTVTAARILKGQKKDKLGPEIPLAMDRFPYVALSKTYNVDK
                     HVPDSGATATAYLCGVKGNFQTIGLSAAARFNQCNTTRGNEVISVMNRAKKAGKSVGVV
                     TTTRVQHASPAGTYAHTVNRNWYSDADVPASARQEGCQDIATQLISNMDIDVILGGGRK
                     YMFRMGTPDPEYPDDYSQGGTRLDGKNLVQEWLAKRQGARYVWNRTELMQASLDPSVTH
                     LMGLFEPGDMKYEIHRDSTLDPSLMEMTEAALRLLIRNPRGFFLFVEGGRIDHGHHESR
                     AYRALTETIMFDDAIERAGQLTSEEDTLSLVTADHSHVFSFGGYPLRGSSIFGLAPGKA
                     RDRKAYTVLLYGNGPGYVLKDGARPDVTESESGSPEYRQQSAVPLDEETHAGEDVAVFA
                     RGPQAHLVHGVQEQTFIAHVMAFAACLEPYTACDLAPPAGTTD"
     primer_bind     complement(1369..1405)
                     /label=oSCL545
     primer_bind     1386..1414
                     /label=oSCL544
     primer_bind     complement(2202..2233)
                     /label=oSCL546
     primer_bind     2214..2246
                     /label=oSCL547
     primer_bind     2299..2329
                     /label=Oscl50-1
     primer_bind     complement(2299..2329)
                     /label=Oscl50-2
     primer_bind     complement(2688..2737)
                     /label=oSCL795
     primer_bind     complement(2722..2769)
                     /label=oSCL475
     primer_bind     complement(2722..2757)
                     /label=oSCL2461
     primer_bind     complement(2724..2742)
                     /label=oSCL083
     primer_bind     complement(2724..2741)
                     /label=oSCL548
     primer_bind     complement(2724..2740)
                     /label=Oscl31-4
     primer_bind     complement(2724..2740)
                     /label=oSCL457
     primer_bind     complement(2724..2737)
                     /label=oSCL776
     polyA_signal    2732..2740
                     /label=SV40 late polyA
     sig_peptide     2738..2797
                     /label=P2A
     primer_bind     2738..2757
                     /label=oSCL1110
     CDS             2741..2797
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             2798..3127
                     /label=mINS
     primer_bind     complement(3110..3150)
                     /label=oSCL1847
     primer_bind     complement(3110..3136)
                     /label=oSCL1315
     primer_bind     complement(3110..3130)
                     /label=oSCL029
     primer_bind     complement(3110..3130)
                     /label=oSCL789
     primer_bind     complement(3110..3130)
                     /label=oSCL1111
     primer_bind     3128..3165
                     /label=oSCL1795
     polyA_signal    3137..3258
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     3137..3165
                     /label=OXB007
     primer_bind     complement(3148..3167)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3161..3181
                     /label=PSG5-R(SV40-R)
     primer_bind     3202..3221
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(3229..3258)
                     /label=OXB016
     primer_bind     complement(3233..3258)
                     /label=OXB008
     primer_bind     complement(3242..3264)
                     /label=oSCL1801
     primer_bind     complement(3242..3258)
                     /label=oSCL1174
     primer_bind     complement(3242..3258)
                     /label=oSCL1796
     misc_feature    3263..3850
                     /label=CMV
     primer_bind     3263..3284
                     /label=oSCL1800
     enhancer        3266..3645
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3646..3849
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(3819..3850)
                     /label=OXB018
     primer_bind     3826..3850
                     /label=oSCL1791
     primer_bind     3829..3849
                     /label=oSCL1013
     primer_bind     3851..3870
                     /label=T7
     promoter        3851..3869
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     3857..3906
                     /label=oSCL2413
     primer_bind     3880..3906
                     /label=oSCL2381
     regulatory      3880..3889
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3889..4041
                     /codon_start=1
                     /label=MS2-N55K
                     /translation="ASNFTQFVLVDNGGTGDVTVAPSNFANGIAEWISSNSRSQAYKVT
                     CSVRQS"
     CDS             4042..4452
                     /codon_start=1
                     /label=LOV2
                     /translation="LERIEKNFVITDPRLPDNPIIFASDSFLQLTEYSREEILGRNCRF
                     LQGPETDRATVRKIRDAIDNQTEVTVQLINYTKSGKKFWNLFHLQPMRDQKGDVQYFIG
                     VQLDGTEHVRDAAEREGVMLIKKTAENIDEAAG"
     primer_bind     4046..4088
                     /label=oSCL2408
     primer_bind     complement(4046..4065)
                     /label=oSCL2407
     CDS             4066..4068
                     /codon_start=1
                     /label=V416I
                     /translation="V"
     primer_bind     4382..4424
                     /label=oSCL2410
     primer_bind     complement(4382..4401)
                     /label=oSCL2409
     CDS             4402..4404
                     /codon_start=1
                     /label=G528A
                     /translation="G"
     primer_bind     4412..4455
                     /label=oSCL2412
     primer_bind     complement(4412..4431)
                     /label=oSCL2411
     CDS             4432..4434
                     /codon_start=1
                     /label=N538E
                     /translation="N"
     CDS             4453..4680
                     /codon_start=1
                     /label=MS2-N55K
                     /translation="QKRKYTIKVEVPKVATQTVGGVELPVAAWRSYLNMELTIPIFATN
                     SDCELIVKAMQGLLKDGNPIPSAIAANSGIY"
     primer_bind     complement(4661..4703)
                     /label=oSCL2415
     primer_bind     complement(4661..4683)
                     /label=oSCL2383
     primer_bind     complement(4661..4680)
                     /label=oSCL2382
     primer_bind     4661..4680
                     /label=oSCL2384
     misc_feature    4690..4738
                     /label=HSV TK poly(A) signal
     polyA_signal    4690..4738
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     complement(4714..4746)
                     /label=oSCL1799
     repeat_region   4747..4887
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   4747..4876
                     /label=AAV2 ITR
     rep_origin      4962..5417
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5049..5068)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5259..5280
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5434..5453)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5553..5575
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5613..5631)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5699..5803
                     /gene="bla"
                     /label=AmpR promoter
     CDS             5804..6664
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6022..6041)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6835..7423
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7324..7343
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggcctcta gagttgacat tgattattga ctagttatta atagtaatca
      181 attacggggt cattagttca tagcccatat atggagttcc gcgttacata acttacggta
      241 aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat
      301 gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg
      361 taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac
      421 gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt
      481 cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat gcggttttgg
      541 cagtacatca atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc
      601 attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt
      661 aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata
      721 agcagagctc cttgaaccgg taagaattgc aacaaacaaa tcaccaccgt gaggatcacc
      781 cacggtggtg aaagccttag cctagccgtg aggatcaccc acggtaggca caagaaattc
      841 tacccgtgag gatcacccac gggtagaatc acaaagacta cagccgtgag gatcacccac
      901 ggctgtagac tcaaacttaa aaaggatatc ttcagcaaac tgatcggaga cgagtccgtc
      961 tcaaattgca acaaacaaat caccaccgtg aggatcaccc acggtggtga aagccttagc
     1021 ctagccgtga ggatcaccca cggtaggcac aagaaattct acccgtgagg atcacccacg
     1081 ggtagaatca caaagactac agccgtgagg atcacccacg gctgtagact caaacttaaa
     1141 aaggatatct tcagcaaact gaattcagct tagcgccacc atgctgctgc tgctgctgct
     1201 gctgggcctg aggctacagc tctccctggg catcatccca gttgaggagg agaacccgga
     1261 cttctggaac cgcgaggcag ccgaggccct gggtgccgcc aagaagctgc agcctgcaca
     1321 gacagccgcc aagaacctca tcatcttcct gggcgatggg atgggggtgt ctacggtgac
     1381 agctgccaga atcctaaaag ggcagaagaa ggacaaactg gggcctgaga tacccctggc
     1441 catggaccgc ttcccatatg tggctctgtc caagacatac aatgtagaca aacatgtgcc
     1501 agacagtgga gccacagcca cggcctacct gtgcggggtc aagggcaact tccagaccat
     1561 tggcttgagt gcagccgccc gctttaacca gtgcaacacg acacgcggca acgaggtcat
     1621 ctccgtgatg aatcgggcca agaaagcagg gaagtcagtg ggagtggtaa ccaccacacg
     1681 agtgcagcac gcctcgccag ccggcaccta cgcccacacg gtgaaccgca actggtactc
     1741 ggacgccgac gtgcctgcct cggcccgcca ggaggggtgc caggacatcg ctacgcagct
     1801 catctccaac atggacattg acgtgatcct aggtggaggc cgaaagtaca tgtttcgcat
     1861 gggaacccca gaccctgagt acccagatga ctacagccaa ggtgggacca ggctggacgg
     1921 gaagaatctg gtgcaggaat ggctggcgaa gcgccagggt gcccggtatg tgtggaaccg
     1981 cactgagctc atgcaggctt ccctggaccc gtctgtgacc catctcatgg gtctctttga
     2041 gcctggagac atgaaatacg agatccaccg agactccaca ctggacccct ccctgatgga
     2101 gatgacagag gctgccctgc gcctgctgat caggaacccc cgcggcttct tcctcttcgt
     2161 ggagggtggt cgcatcgacc atggtcatca tgaaagcagg gcttaccggg cactgactga
     2221 gaccatcatg ttcgacgacg ccattgagag ggcgggccag ctcaccagcg aggaggacac
     2281 gctgagcctc gtcactgccg accactccca cgtgttctcc ttcggaggct accccctgcg
     2341 agggagctcc atcttcgggc tggcccctgg caaggcccgg gacaggaagg cctacacggt
     2401 cctcctatac ggaaacggtc caggctatgt gctcaaggac ggcgcccggc cggatgttac
     2461 cgagagcgag agcgggagcc ccgagtatcg gcagcagtca gcagtgcccc tggacgaaga
     2521 gacccacgca ggcgaggacg tggcggtgtt cgcgcgcggc ccgcaggcgc acctggttca
     2581 cggcgtgcag gagcagacct tcatagcgca cgtcatggcc ttcgccgcct gcctggagcc
     2641 ctacaccgcc tgcgacctgg cgccccccgc cggcaccacc gacgccgcgc acccgggtta
     2701 cagcagagtc ggggcggccg gccgcttcga gcagacagga gcaaccaact tttccctgct
     2761 gaagcaggca ggcgacgtgg aggagaatcc tggacccatg gccctgtgga tgcgcttcct
     2821 gcccctgctg gccctgctcg tcctctggga gcccaagcct gcccaggctt ttgtcaaaca
     2881 gcacctttgt ggtcctcacc tggtggaggc tctgtacctg gtgtgtgggg aacgtggttt
     2941 cttctacaca cccaagtccc gtcgtaaaag ggaggacccg caagtgccac aactggagct
     3001 gggtggaggc ccggaggccg gggatcttca gaccttggca ctggaggttg cccggcagaa
     3061 gcgtggcatt gtggatcagt gctgcaccag catctgctcc ctctaccaac tggagaacta
     3121 ctgcaactga ctcgagtaag atacattgat gagtttggac aaaccacaac tagaatgcag
     3181 tgaaaaaaat gctttatttg tgaaatttgt gatgctattg ctttatttgt aaccattata
     3241 agctgcaata aacaagttac gcgttgacat tgattattga ctagttatta atagtaatca
     3301 attacggggt cattagttca tagcccatat atggagttcc gcgttacata acttacggta
     3361 aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat
     3421 gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg
     3481 taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac
     3541 gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt
     3601 cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat gcggttttgg
     3661 cagtacatca atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc
     3721 attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt
     3781 aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata
     3841 agcagagctc taatacgact cactataggg aagctttaag ccaccatggc tagcaacttc
     3901 acacagttcg tgctggtgga caacggcggc accggcgacg tgaccgtggc ccctagcaac
     3961 ttcgccaacg gcatcgccga gtggatcagc agcaacagca gaagccaagc ctacaaggtg
     4021 acctgcagcg tgagacagag cctggagaga atcgagaaga acttcgtgat caccgaccct
     4081 agactgcccg acaaccccat catcttcgct agcgacagct tcctgcagct gaccgagtac
     4141 agcagagagg agatcctggg cagaaactgc agattcctgc aaggccccga gaccgacaga
     4201 gccaccgtga gaaagatcag agacgccatc gacaatcaga ccgaggtgac cgtgcagctg
     4261 atcaactaca ccaagagcgg caagaagttc tggaacctgt tccacctgca gcccatgaga
     4321 gatcagaagg gcgacgtgca gtacttcatc ggcgtgcagc tggacggcac cgagcacgtg
     4381 agagacgccg ccgagagaga gggcgtgatg ctgatcaaga agaccgccga gaacatcgac
     4441 gaggccgccg ggcagaagag aaagtacacc atcaaagtcg aggtgcccaa ggtggccacc
     4501 caaaccgtgg gcggcgtgga gctgcccgtg gccgcctgga gaagctacct gaacatggag
     4561 ctgaccatcc ccatcttcgc caccaacagc gactgcgagc tgatcgtgaa ggccatgcaa
     4621 ggcctgctga aggacggcaa ccccattcct agcgccatcg ctgctaacag cggcatttat
     4681 taacaattgc ggcaataaaa agacagaata aaacgcacgg gtgttgggtc gtttgttcgc
     4741 ggccgcagga acccctagtg atggagttgg ccactccctc tctgcgcgct cgctcgctca
     4801 ctgaggccgg gcgaccaaag gtcgcccgac gcccgggctt tgcccgggcg gcctcagtga
     4861 gcgagcgagc gcgcagctgc ctgcaggggc gcctgatgcg gtattttctc cttacgcatc
     4921 tgtgcggtat ttcacaccgc atacgtcaaa gcaaccatag tacgcgccct gtagcggcgc
     4981 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgcctt
     5041 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
     5101 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
     5161 ccccaaaaaa cttgatttgg gtgatggttc acgtagtggg ccatcgccct gatagacggt
     5221 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg
     5281 aacaacactc aactctatct cgggctattc ttttgattta taagggattt tgccgatttc
     5341 ggtctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat
     5401 attaacgttt acaattttat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt
     5461 aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc
     5521 ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc
     5581 accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat ttttataggt
     5641 taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg
     5701 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
     5761 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt
     5821 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
     5881 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga
     5941 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat
     6001 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca
     6061 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt
     6121 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac
     6181 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct
     6241 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga
     6301 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac
     6361 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat
     6421 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg
     6481 ctggtttatt gctgataaat ctggagccgg tgagcgtgga agccgcggta tcattgcagc
     6541 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
     6601 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
     6661 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta
     6721 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
     6781 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     6841 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     6901 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     6961 agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa
     7021 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     7081 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     7141 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     7201 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     7261 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     7321 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     7381 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     7441 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgt
//