pQZ197

LOCUS       pQZ197            7736 bp DNA     circular SYN 16-JUL-2026
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 7736)
  AUTHORS   Liuqi Zhao
  TITLE     Direct Submission
  JOURNAL   Exported Sep 3, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..7736
                     /mol_type="other DNA"
                     /organism="recombinant plasmid"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     235..278
                     /label=oQZ313
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(827..852)
                     /label=oQZ314
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     876..960
                     /label=QL19 AML-F
     primer_bind     910..934
                     /label=QL17 ETO-F
     primer_bind     919..966
                     /label=oQZ514
     gene            941..1447
                     /gene="RUNX1"
                     /gene_synonym="AML1; AML1-EVI-1; AMLCR1; CBF2alpha; CBFA2;
                     EVI-1; PEBP2aB; PEBP2alpha"
                     /label=RUNX1
                     /note="RUNX family transcription factor 1; Derived by
                     automated computational analysis using gene prediction 
                     method: Gnomon. Supporting evidence includes similarity to:
                     9 mRNAs, 19 ESTs, 966 long SRA reads, 11 Proteins, and 100%
                     coverage of the annotated genomic feature by RNAseq 
                     alignments, including 11 samples with support for all 
                     annotated introns"
                     /db_xref="GeneID:861"
                     /db_xref="HGNC:HGNC:10471"
                     /db_xref="MIM:151385"
     CDS             941..1447
                     /codon_start=1
                     /gene="RUNX1"
                     /gene_synonym="AML1; AML1-EVI-1; AMLCR1; CBF2alpha; CBFA2;
                     EVI-1; PEBP2aB; PEBP2alpha"
                     /product="runt-related transcription factor 1 isoform X1"
                     /label=RUNX1
                     /db_xref="GeneID:861"
                     /db_xref="HGNC:HGNC:10471"
                     /db_xref="MIM:151385"
                     /protein_id="XP_011528068.1"
                     /translation="MASDSIFESFPSYPQCFMRECILGMNPSRDVHDASTSRRFTPPST
                     ALSPGKMSEALPLGAPDAGAALAGKLRSGDRSMVEVLADHPGELVRTDSPNFLCSVLPT
                     HWRCNKTLPIAFKVVALGDVPDGTLVTVMAGNDENYSAELRNATAAMKNQVARFNDLRF
                     VGRSGR"
     exon            941..997
                     /label=2
     exon            998..1037
                     /label=3
     exon            1038..1291
                     /label=4
     exon            1292..1447
                     /label=5
     primer_bind     complement(1425..1476)
                     /label=oQZ515
     primer_bind     1425..1476
                     /label=oQZ516
     primer_bind     complement(1428..1447)
                     /label=QL20 AML-R
     primer_bind     1446..1476
                     /label=oQZ520
     CDS             1448..3259
                     /codon_start=1
                     /gene="RUNX1T1"
                     /gene_synonym="AML1-MTG8; AML1T1; CBFA2T1; CDR; ETO; MTG8;
                     ZMYND2"
                     /product="protein CBFA2T1 isoform X1"
                     /label=RUNX1T1
                     /db_xref="GeneID:862"
                     /db_xref="HGNC:HGNC:1535"
                     /db_xref="MIM:133435"
                     /protein_id="XP_011515653.1"
                     /translation="ISVKRNTWRALSLVIGDCRKKGNFEYCQDRTEKHSTMPDSPVDVK
                     TQSRLTPPTMPPPPTTQGAPRTSSFTPTTLTNGTSHSPTALNGAPSPPNGFSNGPSSSS
                     SSSLANQQLPPACGARQLSKLKRFLTTLQQFGNDISPEIGERVRTLVLGLVNSTLTIEE
                     FHSKLQEATNFPLRPFVIPFLKANLPLLQRELLHCARLAKQNPAQYLAQHEQLLLDAST
                     TSPVDSSELLLDVNENGKRRTPDRTKENGFDREPLHSEHPSKRPCTISPGQRYSPNNGL
                     SYQPNGLPHPTPPPPQHYRLDDMAIAHHYRDSYRHPSHRDLRDRNRPMGLHGTRQEEMI
                     DHRLTDREWAEEWKHLDHLLNCIMDMVEKTRRSLTVLRRCQEADREELNYWIRRYSDAE
                     DLKKGGGSSSSHSRQQSPVNPDPVALDAHREFLHRPASGYVPEEIWKKAEEAVNEVKRQ
                     AMTELQKAVSEAERKAHDMITTERAKMERTVAEAKRQAAEDALAVINQQEDSSESCWNC
                     GRKASETCSGCNTARYCGSFCQHKDWEKHHHICGQTLQAQQQGDTPAVSSSVTPNSGAG
                     SPMDTPPAATPRSTTPGTPSTIETTPR"
     gene            1448..3259
                     /gene="RUNX1T1"
                     /gene_synonym="AML1-MTG8; AML1T1; CBFA2T1; CDR; ETO; MTG8;
                     ZMYND2"
                     /label=RUNX1T1
                     /note="RUNX1 partner transcriptional co-repressor 1;
                     Derived by automated computational analysis using gene 
                     prediction method: Gnomon. Supporting evidence includes 
                     similarity to: 7 mRNAs, 20 ESTs, 629 long SRA reads, 34 
                     Proteins, and 97% coverage of the annotated genomic feature
                     by RNAseq alignments, including 5 samples with support for 
                     all annotated introns"
                     /db_xref="GeneID:862"
                     /db_xref="HGNC:HGNC:1535"
                     /db_xref="MIM:133435"
     exon            1448..1532
                     /label=2
     primer_bind     1448..1467
                     /label=QL17 ETO-F
     exon            1533..1670
                     /label=3
     primer_bind     1566..1585
                     /label=ETO1
     exon            1671..1912
                     /label=4
     exon            1913..2002
                     /label=5
     exon            2003..2184
                     /label=6
     exon            2185..2435
                     /label=7
     exon            2436..2521
                     /label=8
     exon            2522..2723
                     /label=9a
     primer_bind     complement(2704..2723)
                     /label=oQZ518
     exon            2724..2792
                     /label=9
     exon            2793..2983
                     /label=10
     exon            2984..3259
                     /label=11
     primer_bind     complement(3237..3275)
                     /label=oQZ517
     primer_bind     complement(3240..3278)
                     /label=QL18 ETO-R
     primer_bind     complement(3257..3275)
                     /label=oQZ518
     primer_bind     3319..3337
                     /label=oQZ311
     polyA_signal    3336..3560
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(3557..3579)
                     /label=oQZ312
     primer_bind     3565..3579
                     /label=oQZ313
     rep_origin      3606..4034
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        4048..4377
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4228..4363
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4444..5238
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     polyA_signal    5412..5533
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5582..5598)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    5606..5622
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(5630..5660)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5675..5696
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(5984..6569)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(6740..7600)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(7601..7705)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gtttaaactt aagcttggta ccgagctcgg atccgccacc atggcttcag acagcatatt
      961 tgagtcattt ccttcgtacc cacagtgctt catgagagaa tgcatacttg gaatgaatcc
     1021 ttctagagac gtccacgatg ccagcacgag ccgccgcttc acgccgcctt ccaccgcgct
     1081 gagcccaggc aagatgagcg aggcgttgcc gctgggcgcc ccggacgccg gcgctgccct
     1141 ggccggcaag ctgaggagcg gcgaccgcag catggtggag gtgctggccg accacccggg
     1201 cgagctggtg cgcaccgaca gccccaactt cctctgctcc gtgctgccta cgcactggcg
     1261 ctgcaacaag accctgccca tcgctttcaa ggtggtggcc ctaggggatg ttccagatgg
     1321 cactctggtc actgtgatgg ctggcaatga tgaaaactac tcggctgagc tgagaaatgc
     1381 taccgcagcc atgaagaacc aggttgcaag atttaatgac ctcaggtttg tcggtcgaag
     1441 tggaagaata tctgtcaaaa gaaacacttg gagagcactg agtttagtaa taggtgactg
     1501 ccggaaaaaa gggaactttg aatattgtca agatcgtact gagaagcact ccacaatgcc
     1561 agactcacct gtggatgtga agacgcaatc taggctgact cctccaacaa tgccacctcc
     1621 cccaactact caaggagctc caagaaccag ttcatttaca ccgacaacgt taactaatgg
     1681 cacgagccat tctcctacag ccttgaatgg cgccccctca ccacccaatg gcttcagcaa
     1741 tgggccttcc tcttcttcct cctcctctct ggctaatcaa cagctgcccc cagcctgtgg
     1801 tgccaggcaa ctcagcaagc tgaaaaggtt ccttactacc ctgcagcagt ttggcaatga
     1861 catttcaccc gagataggag aaagagttcg caccctcgtt ctgggactag tgaactccac
     1921 tttgacaatt gaagaatttc attccaaact gcaagaagct actaacttcc cactgagacc
     1981 ttttgtcatc ccatttttga aggccaactt gcccctgctg cagcgtgagc tcctccactg
     2041 cgcaagactg gccaaacaga accctgccca gtacctcgcc cagcatgaac agctgcttct
     2101 ggatgccagc accacctcac ctgttgactc ctcagagctg cttctcgatg tgaacgaaaa
     2161 cgggaagagg cgaactccag acagaaccaa agaaaatggc tttgacagag agcctttgca
     2221 ctcagaacat ccaagcaagc gaccatgcac tattagccca ggccagcggt acagtccaaa
     2281 taacggctta tcctaccagc ccaatggcct gcctcaccct accccacctc cacctcagca
     2341 ttaccgtttg gatgatatgg ccattgccca ccactacagg gactcctatc gacaccccag
     2401 ccacagggac ctcagggaca gaaacagacc tatggggttg catggcacac gtcaagaaga
     2461 aatgattgat cacagactaa cagacagaga atgggcagaa gagtggaaac atcttgacca
     2521 tctgttaaac tgcataatgg acatggtaga aaaaacaagg cgatctctca ccgtactaag
     2581 gcggtgtcaa gaagcagacc gggaagaatt gaattactgg atccggcggt acagtgacgc
     2641 cgaggactta aaaaaaggtg gcggcagtag cagcagccac tctaggcagc agagtcccgt
     2701 caacccagac ccagttgcac tagacgcgca tcgggaattc cttcacaggc ctgcgtctgg
     2761 atacgtgcca gaggagatct ggaagaaagc tgaggaggcc gtcaatgagg tgaagcgcca
     2821 ggcgatgacg gagctgcaga aggccgtgtc tgaggcggag cggaaagccc acgacatgat
     2881 cacaacagag agggccaaga tggagcgcac ggtcgccgag gccaaacggc aggcggcgga
     2941 ggacgcactg gcagttatca atcagcagga ggattcaagc gagagttgct ggaattgtgg
     3001 ccgtaaagcg agtgaaacct gcagtggctg taacacagcc cgatactgtg gctcattttg
     3061 ccagcacaaa gactgggaga agcaccatca catctgtgga cagaccctgc aggcccagca
     3121 gcagggagac acacctgcag tcagctcctc tgtcacgccc aacagcgggg ctgggagccc
     3181 gatggacaca ccaccagcag ccactccgag gtcaaccacc ccgggaaccc cttccaccat
     3241 agagacaacc cctcgctagg aattctgcag atatccagca cagtggcggc cgctcgagtc
     3301 tagagggccc gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt gccagccatc
     3361 tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct
     3421 ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg
     3481 gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg
     3541 ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc agctggggct ctagggggta
     3601 tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt
     3661 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct
     3721 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg
     3781 atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag
     3841 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa
     3901 tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga
     3961 tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa
     4021 atttaacgcg aattaattct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc
     4081 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga
     4141 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
     4201 accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat
     4261 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctctgcc
     4321 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag
     4381 ctcccgggag cttgtatatc cattttcgga tctgatcaag agacaggatg aggatcgttt
     4441 cgcatgattg aacaagatgg attgcacgca ggttctccgg ccgcttgggt ggagaggcta
     4501 ttcggctatg actgggcaca acagacaatc ggctgctctg atgccgccgt gttccggctg
     4561 tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa
     4621 ctgcaggacg aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct
     4681 gtgctcgacg ttgtcactga agcgggaagg gactggctgc tattgggcga agtgccgggg
     4741 caggatctcc tgtcatctca ccttgctcct gccgagaaag tatccatcat ggctgatgca
     4801 atgcggcggc tgcatacgct tgatccggct acctgcccat tcgaccacca agcgaaacat
     4861 cgcatcgagc gagcacgtac tcggatggaa gccggtcttg tcgatcagga tgatctggac
     4921 gaagagcatc aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc gcgcatgccc
     4981 gacggcgagg atctcgtcgt gacccatggc gatgcctgct tgccgaatat catggtggaa
     5041 aatggccgct tttctggatt catcgactgt ggccggctgg gtgtggcgga ccgctatcag
     5101 gacatagcgt tggctacccg tgatattgct gaagagcttg gcggcgaatg ggctgaccgc
     5161 ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt
     5221 cttgacgagt tcttctgagc gggactctgg ggttcgaaat gaccgaccaa gcgacgccca
     5281 acctgccatc acgagatttc gattccaccg ccgccttcta tgaaaggttg ggcttcggaa
     5341 tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg ctggagttct
     5401 tcgcccaccc caacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
     5461 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
     5521 tcaatgtatc ttatcatgtc tgtataccgt cgacctctag ctagagcttg gcgtaatcat
     5581 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag
     5641 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg
     5701 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
     5761 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
     5821 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     5881 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     5941 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     6001 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     6061 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     6121 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     6181 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     6241 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     6301 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     6361 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     6421 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     6481 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tttttttgtt tgcaagcagc
     6541 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     6601 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
     6661 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
     6721 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
     6781 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
     6841 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
     6901 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     6961 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     7021 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
     7081 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
     7141 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
     7201 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
     7261 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
     7321 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
     7381 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
     7441 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
     7501 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
     7561 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
     7621 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
     7681 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtc
//