| 质粒名称: | pAAVLINKv1-pCALM1-Cre-3'-SMARCA2-FLAG |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001968 |
| 产品用途: | 3' AAVLINK plasmid for SMARCA2 expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒 |
| 启动子: | pCALM1 |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | SMARCA2 |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
pAAVLINKv1-pCALM1-Cre-3'-SMARCA2-FLAG
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAVLINKv1-pCALM1-Cre-3'-SMARCA2-FLAG载体质粒基本信息
pAAVLINKv1-pCALM1-Cre-3'-SMARCA2-FLAG质粒图谱载体图谱和pAAVLINKv1-pCALM1-Cre-3'-SMARCA2-FLAG载体序列质粒序列多克隆位点信息
pAAVLINKv1-pCALM1-Cre-3'-SMARCA2-FLAG质粒载体简介
pAAVLINKv1-pCALM1-Cre-3'-SMARCA2-FLAG质粒序列载体序列
LOCUS pAAVLINKv1-pCALM1-Cre-3'-SMARCA2-FLAG 6390 bp DNA circular SYN 10-AUG-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6390)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported Jun 19, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6390
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
/note="color: #ffe4c4"
promoter 145..265
/label=pCALM1
CDS 277..1308
/codon_start=1
/product="site-specific recombinase"
/label=Cre
/note="Cre is a site-specific recombinase from
bacteriophage P1. Recombination occurs at loxP sequences."
/translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
DGD"
intron 1309..1349
/label=Splicing Donor
protein_bind 1356..1389
/label=lox2272-JTZ17
/bound_moiety="Cre recombinase"
/note="Right element (RE) mutant of loxP (Araki et al.,
2010). Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
intron 1390..1481
/label=Splicing Acceptor
/note="Splicing acceptor"
CDS 1482..3103
/codon_start=1
/gene="SMARCA2"
/gene_synonym="BAF190; BIS; BRM; hBRM; hSNF2a; NCBRS;SNF2;
SNF2L2; SNF2LA; Sth1p; SWI2"
/product="probable global transcription activator
SNF2L2isoform a"
/label=SMARCA2
/note="isoform a is encoded by transcript variant 1;global
transcription activator homologous sequence; SNF2-alpha;
sucrose nonfermenting 2-like protein 2; SNF2/SWI2-like
protein 2; SWI/SNF-related matrix-associated
actin-dependent regulator of chromatin a2; brahma homolog;
probable global transcription activator SNF2L2; BAF190B;
protein brahma homolog; BRG1-associated factor 190B;
ATP-dependent helicase SMARCA2; putative global
transcription activator SNF2L2"
/db_xref="GI:48255900"
/db_xref="CCDS:CCDS34977.1"
/db_xref="GeneID:6595"
/db_xref="HGNC:HGNC:11098"
/db_xref="MIM:600014"
/protein_id="NP_003061.3"
/translation="KFELLDRILPKLRATNHRVLLFCQMTSLMTIMEDYFAFRNFLYLR
LDGTTKSEDRAALLKKFNEPGSQYFIFLLSTRAGGLGLNLQAADTVVIFDSDWNPHQDL
QAQDRAHRIGQQNEVRVLRLCTVNSVEEKILAAAKYKLNVDQKVIQAGMFDQKSSSHER
RAFLQAILEHEEENEEEDEVPDDETLNQMIARREEEFDLFMRMDMDRRREDARNPKRKP
RLMEEDELPSWIIKDDAEVERLTCEEEEEKIFGRGSRQRRDVDYSDALTEKQWLRAIED
GNLEEMEEEVRLKKRKRRRNVDKDPAKEDVEKAKKRRGRPPAEKLSPNPPKLTKQMNAI
IDTVINYKDRCNVEKVPSNSQLEIEGNSSGRQLSEVFIQLPSRKELPEYYELIRKPVDF
KKIKERIRNHKYRSLGDLEKDVMLLCHNAQTFNLEGSQIYEDSIVLQSVFKSARQKIAK
EEESEDESNEEEEEEDEEESESEAKSVKVKIKLNKKDDKGRDKGKGKKRPNRGKAKPVV
SDFDSDEEQDEREQSEGSGTDDE"
misc_feature 2004..2009
/label=SDS
misc_feature 2160..2165
/label=SDS
misc_feature 2175..2180
/label=SDS
misc_feature 2229..2234
/label=SDS
misc_feature 2889..2894
/label=SDS
CDS 3110..3175
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
terminator 3179..3610
/label=W3SL
/note="Short WPRE and SV40 polyA"
misc_feature 3323..3328
/label=SDS
repeat_region 3653..3793
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 3653..3782
/label=AAV2 ITR
repeat_region 3654..3794
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
rep_origin 3868..4323
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 4605..4709
/gene="bla"
/label=AmpR promoter
CDS 4710..5570
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 5741..6329
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtcgctct gcgcgaggcg gctccgccgc ggcggaggga
181 tacggcgcac catatatata tcgcggggcg cagactcgcg ctccggcagt ggtgctggga
241 gtgtcgtgga cgccgtgccg ttactcgtac gccaccatgt ccaatttact gaccgtacac
301 caaaatttgc ctgcattacc ggtcgatgca acgagtgatg aggttcgcaa gaacctgatg
361 gacatgttca gggatcgcca ggcgttttct gagcatacct ggaaaatgct tctgtccgtt
421 tgccggtcgt gggcggcatg gtgcaagttg aataaccgga aatggtttcc cgcagaacct
481 gaagatgttc gcgattatct tctatatctt caggcgcgcg gtctggcagt aaaaaccatc
541 cagcaacatt tgggccagct aaacatgctt catcgtcggt ccgggctgcc acgaccaagt
601 gacagcaatg ctgtttcact ggttatgcgg cggatccgaa aagaaaacgt tgatgccggt
661 gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt tcgaccaggt tcgttcactc
721 atggaaaata gcgatcgctg ccaggatata cgtaatctgg catttctggg gattgcttat
781 aacaccctgt tacgtatagc cgaaattgcc aggatcaggg ttaaagatat ctcacgtact
841 gacggtggga gaatgttaat ccatattggc agaacgaaaa cgctggttag caccgcaggt
901 gtagagaagg cacttagcct gggggtaact aaactggtcg agcgatggat ttccgtctct
961 ggtgtagctg atgatccgaa taactacctg ttttgccggg tcagaaaaaa tggtgttgcc
1021 gcgccatctg ccaccagcca gctatcaact cgcgccctgg aagggatttt tgaagcaact
1081 catcgattga tttacggcgc taaggatgac tctggtcaga gatacctggc ctggtctgga
1141 cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg ctggagtttc aataccggag
1201 atcatgcaag ctggtggctg gaccaatgta aatattgtaa tgaactatat ccgtaacctg
1261 gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg gcgattaggt aagtatcaag
1321 gttacaagac aggtttaagg agaccaataa ctagtataac ttcgtatagg atactttata
1381 gcaatttatg aaactgggct tgtcgagaca gagaagactc ttgcgtttct gataggcacc
1441 tattggtctt actgacatcc actttgcctt tctctccaca gggaagtttg agctgcttga
1501 tcgtattctg ccaaaattga gagcgactaa tcaccgagtg ctgcttttct gccagatgac
1561 atctctcatg accatcatgg aggattattt tgcttttcgg aacttccttt acctacgcct
1621 tgatggcacc accaagtctg aagatcgtgc tgctttgctg aagaaattca atgaacctgg
1681 atcccagtat ttcattttct tgctgagcac aagagctggt ggcctgggct taaatcttca
1741 ggcagctgat acagtggtca tctttgacag cgactggaat cctcatcagg atctgcaggc
1801 ccaagaccga gctcaccgca tcgggcagca gaacgaggtc cgggtactga ggctctgtac
1861 cgtgaacagc gtggaggaaa agatcctcgc ggccgcaaaa tacaagctga acgtggatca
1921 gaaagtgatc caggcgggca tgtttgacca aaagtcttca agccacgagc ggagggcatt
1981 cctgcaggcc atcttggagc atgaggagga aaatgaggaa gaagatgaag taccggacga
2041 tgagactctg aaccaaatga ttgctcgacg agaagaagaa tttgaccttt ttatgcggat
2101 ggacatggac cggcggaggg aagatgcccg gaacccgaaa cggaagcccc gtttaatgga
2161 ggaggatgag ctgccctcct ggatcattaa ggatgacgct gaagtagaaa ggctcacctg
2221 tgaagaagag gaggagaaaa tatttgggag ggggtcccgc cagcgccgtg acgtggacta
2281 cagtgacgcc ctcacggaga agcagtggct aagggccatc gaagacggca atttggagga
2341 aatggaagag gaagtacggc ttaagaagcg aaaaagacga agaaatgtgg ataaagatcc
2401 tgcaaaagaa gatgtggaaa aagctaagaa gagaagaggc cgccctcccg ctgagaaact
2461 gtcaccaaat ccccccaaac tgacaaagca gatgaacgct atcatcgata ctgtgataaa
2521 ctacaaagat aggtgtaacg tggagaaggt gcccagtaat tctcagttgg aaatagaagg
2581 aaacagttca gggcgacagc tcagtgaagt cttcattcag ttaccttcaa ggaaagaatt
2641 accagaatac tatgaattaa ttaggaagcc agtggatttc aaaaaaataa aggaaaggat
2701 tcgtaatcat aagtaccgga gcctaggcga cctggagaag gatgtcatgc ttctctgtca
2761 caacgctcag acgttcaacc tggagggatc ccagatctat gaagactcca tcgtcttaca
2821 gtcagtgttt aagagtgccc ggcagaaaat tgccaaagag gaagagagtg aggatgaaag
2881 caatgaagag gaggaagagg aagatgaaga agagtcagag tccgaggcaa aatcagtcaa
2941 ggtgaaaatt aagctcaata aaaaagatga caaaggccgg gacaaaggga aaggcaagaa
3001 aaggccaaat cgaggaaaag ccaaacctgt agtgagcgat tttgacagcg atgaggagca
3061 ggatgaacgt gaacagtcag aaggaagtgg gacggatgat gagtgtacag actacaaaga
3121 ccatgacggt gattataaag atcatgacat cgactacaag gatgacgatg acaagtaact
3181 cgagataatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat
3241 gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct
3301 tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttagttct tgccacggcg
3361 gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac
3421 aattccgtgg tgtttatttg tgaaatttgt gatgctattg ctttatttgt aaccatctag
3481 ctttatttgt gaaatttgtg atgctattgc tttatttgta accattataa gctgcaataa
3541 acaagttaac aacaacaatt gcattcattt tatgtttcag gttcaggggg agatgtggga
3601 ggttttttaa agatctggag accggtgtgg cacgtgcgga ccgagcggcc gcaggaaccc
3661 ctagtgatgg agttggccac tccctctctg cgcgctcgct cgctcactga ggccgggcga
3721 ccaaaggtcg cccgacgccc gggctttgcc cgggcggcct cagtgagcga gcgagcgcgc
3781 agctgcctgc aggggcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca
3841 caccgcatac gtcaaagcaa ccatagtacg cgccctgtag cggcgcatta agcgcggcgg
3901 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccttagcg cccgctcctt
3961 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
4021 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg
4081 atttgggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga
4141 cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaact
4201 ctatctcggg ctattctttt gatttataag ggattttgcc gatttcggtc tattggttaa
4261 aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta acgtttacaa
4321 ttttatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagccccgac
4381 acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca
4441 gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga
4501 aacgcgcgag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa
4561 taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
4621 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
4681 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
4741 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
4801 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
4861 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
4921 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc
4981 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
5041 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
5101 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
5161 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
5221 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac
5281 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
5341 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
5401 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
5461 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
5521 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
5581 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
5641 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
5701 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
5761 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
5821 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
5881 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
5941 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
6001 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
6061 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
6121 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
6181 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
6241 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
6301 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
6361 tggccttttg ctggcctttt gctcacatgt
//
pAAVLINKv1-pCALM1-Cre-3'-SMARCA2-FLAG载体图谱质粒图谱pdf版和pAAVLINKv1-pCALM1-Cre-3'-SMARCA2-FLAG载体序列质粒序列等相关资料下载
pAAVLINKv1-pCALM1-Cre-3'-SMARCA2-FLAG质粒载体应用举例
- 上一篇：pAAVLINK-EF1alpha-HA-5'-SMARCA2
- 下一篇：没有了
