| 质粒名称: | pAAVLINK-EF1a-HA-APC-A |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001755 |
| 产品用途: | 5' AAVLINK plasmid for APC expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒 |
| 启动子: | EF1a |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | APC |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
pAAVLINK-EF1a-HA-APC-A
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAVLINK-EF1a-HA-APC-A载体质粒基本信息
pAAVLINK-EF1a-HA-APC-A质粒图谱载体图谱和pAAVLINK-EF1a-HA-APC-A载体序列质粒序列多克隆位点信息
pAAVLINK-EF1a-HA-APC-A质粒载体简介
pAAVLINK-EF1a-HA-APC-A质粒序列载体序列
LOCUS pAAVLINK-EF1a-HA-APC-A 6904 bp DNA circular SYN 21-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6904)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported Jun 5, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6904
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
promoter 146..688
/label=EF1-alpha
/note="Chimeric promoter of ef1a and htlv 5' UTR"
promoter 175..386
/label=EF-1-alpha core promoter
/note="core promoter for human elongation factor
EF-1-alpha"
LTR 399..667
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from human
T-cell leukemia virus (HTLV) type 1"
misc_feature 493..498
/label=SDS
CDS 704..730
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/label=HA
/translation="YPYDVPDYA"
CDS 737..4044
/codon_start=1
/label=APC
/translation="MAAASYDQLLKQVEALKMENSNLRQELEDNSNHLTKLETEASNMK
EVLKQLQGSIEDEAMASSGQIDLLERLKELNLDSSNFPGVKLRSKMSLRSYGSREGSVS
SRSGECSPVPMGSFPRRGFVNGSRESTGYLEELEKERSLLLADLDKEEKEKDWYYAQLQ
NLTKRIDSLPLTENFSLQTDMTRRQLEYEARQIRVAMEEQLGTCQDMEKRAQRRIARIQ
QIEKDILRIRQLLQSQATEAERSSQNKHETGSHDAERQNEGQGVGEINMATSGNGQGST
TRMDHETASVLSSSSTHSAPRRLTSHLGTKVEMVYSLLSMLGTHDKDDMSRTLLAMSSS
QDSCISMRQSGCLPLLIQLLHGNDKDSVLLGNSRGSKEARARASAALHNIIHSQPDDKR
GRREIRVLHLLEQIRAYCETCWEWQEAHEPGMDQDKNPMPAPVEHQICPAVCVLMKLSF
DEEHRHAMNELGGLQAIAELLQVDCEMYGLTNDHYSITLRRYAGMALTNLTFGDVANKA
TLCSMKGCMRALVAQLKSESEDLQQVIASVLRNLSWRADVNSKKTLREVGSVKALMECA
LEVKKESTLKSVLSALWNLSAHCTENKADICAVDGALAFLVGTLTYRSQTNTLAIIESG
GGILRNVSSLIATNEDHRQILRENNCLQTLLQHLKSHSLTIVSNACGTLWNLSARNPKD
QEALWDMGAVSMLKNLIHSKHKMIAMGSAAALRNLMANRPAKYKDANIMSPGSSLPSLH
VRKQKALEAELDAQHLSETFDNIDNLSPKASHRSKQRHKQSLYGDYVFDTNRHDDNRSD
NFNTGNMTVLSPYLNTTVLPSSSSSRGSLDSSRSEKDRSLERERGIGLGNYHPATENPG
TSSKRGLQISTTAAQIAKVMEEVSAIHTSQEDRSSGSTTELHCVTDERNALRRSSAAHT
HSNTYNFTKSENSNRTCSMPYAKLEYKRSSNDSLNSVSSSDGYGKRGQMKPSIESYSED
DESKFCSYGQYPADLAHKIHSANHMDDNDGELDTPINYSLKYSDEQLNSGRQSPSQNER
WARPKHIIEDEIKQSEQRQSRNQSTTYPVYTESTDDKHLKFQPHFGQQECVSPY"
intron 4045..4085
/label=Splicing Donor
repeat_region 4167..4307
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 4167..4296
/label=AAV2 ITR
rep_origin 4382..4837
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 5119..5223
/gene="bla"
/label=AmpR promoter
CDS 5224..6084
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 6255..6843
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtggatct gcgatcgctc cggtgcccgt cagtgggcag
181 agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat tgaacgggtg
241 cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt
301 ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac gttctttttc
361 gcaacgggtt tgccgccaga acacagctga agcttcgagg ggctcgcatc tctccttcac
421 gcgcccgccg ccctacctga ggccgccatc cacgccggtt gagtcgcgtt ctgccgcctc
481 ccgcctgtgg tgcctcctga actgcgtccg ccgtctaggt aagtttaaag ctcaggtcga
541 gaccgggcct ttgtccggcg ctcccttgga gcctacctag actcagccgg ctctccacgc
601 tttgcctgac cctgcttgct caactctacg tctttgtttc gttttctgtt ctgcgccgtt
661 acagatccaa gctgtgaccg gcgcctaccg tacggccacc atgtaccctt atgacgtacc
721 tgactatgct ggatctatgg ctgcagcttc atatgatcag ttgttaaagc aagttgaggc
781 actgaagatg gagaactcaa atcttcgaca agagctagaa gataattcca atcatcttac
841 aaaactggaa actgaggcat ctaatatgaa ggaagtactt aaacaactac aaggaagtat
901 tgaagatgaa gctatggctt cttctggaca gattgattta ttagagcgtc ttaaagagct
961 taacttagat agcagtaatt tccctggagt aaaactgcgg tcaaaaatgt ccctccgttc
1021 ttatggaagc cgggaaggat ctgtatcaag ccgttctgga gagtgcagtc ctgttcctat
1081 gggttcattt ccaagaagag ggtttgtaaa tggaagcaga gaaagtactg gatatttaga
1141 agaacttgag aaagagaggt cattgcttct tgctgatctt gacaaagaag aaaaggaaaa
1201 agactggtat tacgctcaac ttcagaatct cactaaaaga atagatagtc ttcctttaac
1261 tgaaaatttt tccttacaaa cagatatgac cagaaggcaa ttggaatatg aagcaaggca
1321 aatcagagtt gcgatggaag aacaactagg tacctgccag gatatggaaa aacgagcaca
1381 gcgaagaata gccagaattc agcaaatcga aaaggacata cttcgtatac gacagctttt
1441 acagtcccaa gcaacagaag cagagaggtc atctcagaac aagcatgaaa ccggctcaca
1501 tgatgctgag cggcagaatg aaggtcaagg agtgggagaa atcaacatgg caacttctgg
1561 taatggtcag ggttcaacta cacgaatgga ccatgaaaca gccagtgttt tgagttctag
1621 tagcacacac tctgcacctc gaaggctgac aagtcatctg ggaaccaagg tggaaatggt
1681 gtattcattg ttgtcaatgc ttggtactca tgataaggat gatatgtcgc gaactttgct
1741 agctatgtct agctcccaag acagctgtat atccatgcga cagtctggat gtcttcctct
1801 cctcatccag cttttacatg gcaatgacaa agactctgta ttgttgggaa attcccgggg
1861 cagtaaagag gctcgggcca gggccagtgc agcactccac aacatcattc actcacagcc
1921 tgatgacaag agaggcaggc gtgaaatccg agtccttcat cttttggaac agatacgcgc
1981 ttactgtgaa acctgttggg agtggcagga agctcatgaa ccaggcatgg accaggacaa
2041 aaatccaatg ccagctcctg ttgaacatca gatctgtcct gctgtgtgtg ttctaatgaa
2101 actttcattt gatgaagagc atagacatgc aatgaatgaa ctagggggac tacaggccat
2161 tgcagaatta ttgcaagtgg actgtgaaat gtacgggctt actaatgacc actacagtat
2221 tacactaaga cgatatgctg gaatggcttt gacaaacttg acttttggag atgtagccaa
2281 caaggctacg ctatgctcta tgaaaggctg catgagagca cttgtggccc aactaaaatc
2341 tgaaagtgaa gacttacagc aggttattgc aagtgttttg aggaatttgt cttggcgagc
2401 agatgtaaat agtaaaaaga cgttgcgaga agttggaagt gtgaaagcat tgatggaatg
2461 tgctttagaa gttaaaaagg aatcaaccct caaaagcgta ttgagtgcct tatggaattt
2521 gtcagcacat tgcactgaga ataaagctga tatatgtgct gtagatggtg cacttgcatt
2581 tttggttggc actcttactt accggagcca gacaaacact ttagccatta ttgaaagtgg
2641 aggtgggata ttacggaatg tgtccagctt gatagctaca aatgaggacc acaggcaaat
2701 cctaagagag aacaactgtc tacaaacttt attacaacac ttaaaatctc atagtttgac
2761 aatagtcagt aatgcatgtg gaactttgtg gaatctctca gcaagaaatc ctaaagacca
2821 ggaagcatta tgggacatgg gggcagttag catgctcaag aacctcattc attcaaagca
2881 caaaatgatt gctatgggaa gtgctgcagc tttaaggaat ctcatggcaa ataggcctgc
2941 gaagtacaag gatgccaata ttatgtctcc tggctcaagc ttgccatctc ttcatgttag
3001 gaaacaaaaa gccctagaag cagaattaga tgctcagcac ttatcagaaa cttttgacaa
3061 tatagacaat ttaagtccca aggcatctca tcgtagtaag cagagacaca agcaaagtct
3121 ctatggtgat tatgtttttg acaccaatcg acatgatgat aataggtcag acaattttaa
3181 tactggcaac atgactgtcc tttcaccata tttgaatact acagtgttac ccagctcctc
3241 ttcatcaaga ggaagcttag atagttctcg ttctgaaaaa gatagaagtt tggagagaga
3301 acgcggaatt ggtctaggca actaccatcc agcaacagaa aatccaggaa cttcttcaaa
3361 gcgaggtttg cagatctcca ccactgcagc ccagattgcc aaagtcatgg aagaagtgtc
3421 agccattcat acctctcagg aagacagaag ttctgggtct accactgaat tacattgtgt
3481 gacagatgag agaaatgcac ttagaagaag ctctgctgcc catacacatt caaacactta
3541 caatttcact aagtcggaaa attcaaatag gacatgttct atgccttatg ccaaattaga
3601 atacaagaga tcttcaaatg atagtttaaa tagtgtcagt agtagtgatg gttatggtaa
3661 aagaggtcaa atgaaaccct cgattgaatc ctattctgaa gatgatgaaa gtaagttttg
3721 cagttatggt caatacccag ccgacctagc ccataaaata catagtgcaa atcatatgga
3781 tgataatgat ggagaactag atacaccaat aaattatagt cttaaatatt cagatgagca
3841 gttgaactct ggaaggcaaa gtccttcaca gaatgaaaga tgggcaagac ccaaacacat
3901 aatagaagat gaaataaaac aaagtgagca aagacaatca aggaatcaaa gtacaactta
3961 tcctgtttat actgagagca ctgatgataa acacctcaag ttccaaccac attttggaca
4021 gcaggaatgt gtttctccat acaggtaagt atcaaggtta caagacaggt ttaaggagac
4081 caataagcta ttattcgtat agcatacatt atacgaagtt attaagttat ggagaccggt
4141 gtggcacgtg cggaccgagc ggccgcagga acccctagtg atggagttgg ccactccctc
4201 tctgcgcgct cgctcgctca ctgaggccgg gcgaccaaag gtcgcccgac gcccgggctt
4261 tgcccgggcg gcctcagtga gcgagcgagc gcgcagctgc ctgcaggggc gcctgatgcg
4321 gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atacgtcaaa gcaaccatag
4381 tacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
4441 gctacacttg ccagcgcctt agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
4501 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
4561 agtgctttac ggcacctcga ccccaaaaaa cttgatttgg gtgatggttc acgtagtggg
4621 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
4681 ggactcttgt tccaaactgg aacaacactc aactctatct cgggctattc ttttgattta
4741 taagggattt tgccgatttc ggtctattgg ttaaaaaatg agctgattta acaaaaattt
4801 aacgcgaatt ttaacaaaat attaacgttt acaattttat ggtgcactct cagtacaatc
4861 tgctctgatg ccgcatagtt aagccagccc cgacacccgc caacacccgc tgacgcgccc
4921 tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc
4981 tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg
5041 atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc
5101 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
5161 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
5221 agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt
5281 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt
5341 gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc
5401 cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta
5461 tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac
5521 ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa
5581 ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg
5641 atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc
5701 cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg
5761 atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta
5821 gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg
5881 cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg
5941 tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc
6001 tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt
6061 gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt
6121 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
6181 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
6241 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
6301 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
6361 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag
6421 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
6481 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
6541 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
6601 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
6661 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
6721 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
6781 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
6841 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
6901 atgt
//
