| 质粒名称: | pLH-stsgRNA2.1 |
|---|---|
| 出品公司： | Addgene |
| 目录编号: | 64117 |
| 存储实验室: | Thoru Pederson |
|
载体骨架基本信息 |
|
| 载体名称: | pLKO.1 |
| 空载体大小: | 8000 bp |
| 完整质粒大小: | 82801bp |
| 载体类型: | 哺乳细胞、 Lentiviral, CRISPR表达 |
| 筛选标记: | Hygromycin |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | ccdB Survival |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | St1 sgRNA2.1 |
| 物种来源: | Synthetic |
| 启动子: | U6 |
| 克隆方法: | 限制性内切酶 |
| 5’克隆位点: | SbfI(未破坏) |
| 3’克隆位点: | EcoRI(未破坏) |
| 5’测序引物 | cccttcaccgagggcctatttccc |
| 3’测序引物 | CTATTCTTTCCCCTGCACTGTACCC |
| 文献引用方法 | |
| 材料和方法部分: | pLH-stsgRNA2.1 was a gift from Thoru Pederson (Addgene plasmid # 64117 ; http://n2t.net/addgene:64117 ; RRID:Addgene_64117) |
| 参考文献部分: | Multicolor CRISPR labeling of chromosomal loci in human cells. Ma H, Naseri A, Reyes-Gutierrez P, Wolfe SA, Zhang S, Pederson T. Proc Natl Acad Sci U S A. 2015 Mar 10;112(10):3002-7. doi: 10.1073/pnas.1420024112. Epub 2015 Feb 23. 10.1073/pnas.1420024112 PubMed 25713381 |
买家导航
pLH-stsgRNA2.1载体质粒基本信息
pLH-stsgRNA2.1质粒图谱载体图谱和pLH-stsgRNA2.1载体序列质粒序列多克隆位点信息
pLH-stsgRNA2.1质粒载体简介
pLH-stsgRNA2.1质粒序列载体序列
LOCUS pLH-stsgRNA2.1 8280 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION Vector for expression of St1 sgRNA2.1 in mammalian cells.
ACCESSION .
VERSION .
KEYWORDS pLH-stsgRNA2.1
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8280)
AUTHORS Ma H, Naseri A, Reyes-Gutierrez P, Wolfe SA, Zhang S, Pederson T
TITLE Multicolor CRISPR labeling of chromosomal loci in human cells.
JOURNAL Proc Natl Acad Sci U S A. 2015 Mar 10;112(10):3002-7. doi:
10.1073/pnas.1420024112. Epub 2015 Feb 23.
PUBMED 25713381
REFERENCE 2 (bases 1 to 8280)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.biofeng.com
FEATURES Location/Qualifiers
source 1..8280
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind 426..444
/label=hPGK-F
/note="Human PGK promoter, forward primer"
CDS 597..1622
/codon_start=1
/gene="aph(4)-Ia"
/product="aminoglycoside phosphotransferase from E. coli"
/label=HygR
/note="confers resistance to hygromycin"
/translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
E"
primer_bind complement(1646..1662)
/label=KS primer
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(1647..1663)
/label=pBluescriptKS
/note="For pBluescript vector"
LTR 1745..1978
/label=3' LTR (Delta-U3)
/note="self-inactivating 3' long terminal repeat (LTR) from
HIV-1"
polyA_signal 2050..2171
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(2087..2106)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 2141..2160
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
rep_origin 2211..2346
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 2273..2292
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
primer_bind complement(2366..2385)
/label=T7
/note="T7 promoter, forward primer"
promoter complement(2367..2385)
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind complement(2395..2412)
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind complement(2395..2411)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(2404..2426)
/label=M13/pUC Forward
/note="In lacZ gene"
rep_origin 2553..3008
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(2640..2659)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 2850..2871
/label=F1ori-F
/note="F1 origin, forward primer"
promoter 3034..3138
/gene="bla"
/label=AmpR promoter
CDS 3139..3999
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(3357..3376)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 4170..4758
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 4659..4678
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 4912..4929
/label=L4440
/note="L4440 vector, forward primer"
protein_bind 5046..5067
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 5082..5112
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 5120..5136
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 5125..5147
/label=M13/pUC Reverse
/note="In lacZ gene"
primer_bind 5144..5160
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 5144..5160
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind 5179..5199
/label=T3
/note="T3 promoter, forward primer"
promoter 5181..5199
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
promoter 5227..5453
/label=RSV promoter
/note="Rous sarcoma virus enhancer/promoter"
LTR 5454..5634
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
misc_feature 5681..5806
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 6299..6532
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
CDS 6717..6761
/codon_start=1
/product="antigenic peptide corresponding to amino acids
655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
et al., 2013)"
/label=gp41 peptide
/note="recognized by the 2H10 single-chain llama nanobody"
/translation="KNEQELLELDKWASL"
promoter 7059..7299
/label=U6 promoter
/note="RNA polymerase III promoter for human U6 snRNA"
primer_bind 7059..7079
/label=hU6-F
/note="Human U6 promoter, forward primer"
primer_bind 7230..7249
/label=LKO.1 5'
/note="Human U6 promoter, forward primer"
CDS 7648..7953
/codon_start=1
/gene="ccdB"
/product="CcdB, a bacterial toxin that poisons DNA gyrase"
/label=ccdB
/note="Plasmids containing the ccdB gene cannot be
propagated in standard E. coli strains."
/translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
primer_bind 7832..7851
/label=ccdB-fwd
/note="ccdB gene, forward primer"
misc_feature 8111..8228
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
promoter 8277..507
/label=hPGK promoter
/note="human phosphoglycerate kinase 1 promoter"
ORIGIN
1 ttggggttgc gccttttcca aggcagccct gggtttgcgc agggacgcgg ctgctctggg
61 cgtggttccg ggaaacgcag cggcgccgac cctgggtctc gcacattctt cacgtccgtt
121 cgcagcgtca cccggatctt cgccgctacc cttgtgggcc ccccggcgac gcttcctgct
181 ccgcccctaa gtcgggaagg ttccttgcgg ttcgcggcgt gccggacgtg acaaacggaa
241 gccgcacgtc tcactagtac cctcgcagac ggacagcgcc agggagcaat ggcagcgcgc
301 cgaccgcgat gggctgtggc caatagcggc tgctcagcag ggcgcgccga gagcagcggc
361 cgggaagggg cggtgcggga ggcggggtgt ggggcggtag tgtgggccct gttcctgccc
421 gcgcggtgtt ccgcattctg caagcctccg gagcgcacgt cggcagtcgg ctccctcgtt
481 gaccgaatca ccgacctctc tccccagggg gatccaccgg atcaagcttg ccagctgggg
541 cgccctctgg taaggttggg aagccctgca gatctgatcc cggggggcaa tgagatatga
601 aaaagcctga actcaccgcg acgtctgtcg agaagtttct gatcgaaaag ttcgacagcg
661 tctccgacct gatgcagctc tcggagggcg aagaatctcg tgctttcagc ttcgatgtag
721 gagggcgtgg atatgtcctg cgggtaaata gctgcgccga tggtttctac aaagatcgtt
781 atgtttatcg gcactttgca tcggccgcgc tcccgattcc ggaagtgctt gacattgggg
841 aatttagcga gagcctgacc tattgcatct cccgccgtgc acagggtgtc acgttgcaag
901 atctgcctga aaccgaactg cccgctgttc tgcagccggt cgcggaggcc atggatgcga
961 tcgctgcggc cgatcttagc cagacgagcg ggttcggccc attcggaccg caaggaatcg
1021 gtcaatacac tacatggcgt gatttcatat gcgcgattgc tgatccccat gtgtatcact
1081 ggcaaactgt gatggacgac accgtcagtg cgtccgtcgc gcaggctctc gatgagctga
1141 tgctttgggc cgaggactgc cccgaagtcc ggcacctcgt gcacgcggat ttcggctcca
1201 acaatgtcct gacggacaat ggccgcataa cagcggtcat tgactggagc gaggcgatgt
1261 tcggggattc ccaatacgag gtcgccaaca tcttcttctg gaggccgtgg ttggcttgta
1321 tggagcagca gacgcgctac ttcgagcgga ggcatccgga gcttgcagga tcgccgcggc
1381 tccgggcgta tatgctccgc attggtcttg accaactcta tcagagcttg gttgacggca
1441 atttcgatga tgcagcttgg gcgcagggtc gatgcgacgc aatcgtccga tccggagccg
1501 ggactgtcgg gcgtacacaa atcgcccgca gaagcgcggc cgtctggacc gatggctgtg
1561 tagaagtact cgccgatagt ggaaaccgac gccccagcac tcgtccgagg gcaaaggaat
1621 agagtagatg ccgaccggga tctatcgata ccgtcgacct cgaggggggg cccggtacct
1681 ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg
1741 ggactggaag ggctaattca ctcccaacga agaaaagatc tgctttttgc ttgtactggg
1801 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
1861 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
1921 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
1981 agtagttcat gtcatcttat tattcagtat ttataacttg caaagaaatg aatatcagag
2041 agtgagagga acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
2101 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
2161 aatgtatctt atcatgtctg gctctagcta tcccgcccct aactccgccc atcccgcccc
2221 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg
2281 cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg
2341 gaggcctagg gacgtaccca attcgcccta tagtgagtcg tattacgcgc gctcactggc
2401 cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc
2461 agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc
2521 ccaacagttg cgcagcctga atggcgaatg ggacgcgccc tgtagcggcg cattaagcgc
2581 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc
2641 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct
2701 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa
2761 acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc
2821 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact
2881 caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg
2941 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgct
3001 tacaatttag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc
3061 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
3121 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt
3181 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct
3241 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc
3301 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta
3361 tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac
3421 tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc
3481 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac
3541 ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg
3601 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac
3661 gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc
3721 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt
3781 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga
3841 gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc
3901 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag
3961 atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca
4021 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc
4081 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
4141 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
4201 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
4261 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt
4321 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
4381 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
4441 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
4501 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
4561 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
4621 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
4681 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
4741 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
4801 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
4861 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca
4921 gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg
4981 attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac
5041 gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg
5101 gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac
5161 catgattacg ccaagcgcgc aattaaccct cactaaaggg aacaaaagct ggagctgcaa
5221 gcttaatgta gtcttatgca atactcttgt agtcttgcaa catggtaacg atgagttagc
5281 aacatgcctt acaaggagag aaaaagcacc gtgcatgccg attggtggaa gtaaggtggt
5341 acgatcgtgc cttattagga aggcaacaga cgggtctgac atggattgga cgaaccactg
5401 aattgccgca ttgcagagat attgtattta agtgcctagc tcgatacata aacgggtctc
5461 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
5521 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
5581 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
5641 cccgaacagg gacttgaaag cgaaagggaa accagaggag ctctctcgac gcaggactcg
5701 gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga ctggtgagta cgccaaaaat
5761 tttgactagc ggaggctaga aggagagaga tgggtgcgag agcgtcagta ttaagcgggg
5821 gagaattaga tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga aaaaatataa
5881 attaaaacat atagtatggg caagcaggga gctagaacga ttcgcagtta atcctggcct
5941 gttagaaaca tcagaaggct gtagacaaat actgggacag ctacaaccat cccttcagac
6001 aggatcagaa gaacttagat cattatataa tacagtagca accctctatt gtgtgcatca
6061 aaggatagag ataaaagaca ccaaggaagc tttagacaag atagaggaag agcaaaacaa
6121 aagtaagacc accgcacagc aagcggccgc tgatcttcag acctggagga ggagatatga
6181 gggacaattg gagaagtgaa ttatataaat ataaagtagt aaaaattgaa ccattaggag
6241 tagcacccac caaggcaaag agaagagtgg tgcagagaga aaaaagagca gtgggaatag
6301 gagctttgtt ccttgggttc ttgggagcag caggaagcac tatgggcgca gcgtcaatga
6361 cgctgacggt acaggccaga caattattgt ctggtatagt gcagcagcag aacaatttgc
6421 tgagggctat tgaggcgcaa cagcatctgt tgcaactcac agtctggggc atcaagcagc
6481 tccaggcaag aatcctggct gtggaaagat acctaaagga tcaacagctc ctggggattt
6541 ggggttgctc tggaaaactc atttgcacca ctgctgtgcc ttggaatgct agttggagta
6601 ataaatctct ggaacagatt tggaatcaca cgacctggat ggagtgggac agagaaatta
6661 acaattacac aagcttaata cactccttaa ttgaagaatc gcaaaaccag caagaaaaga
6721 atgaacaaga attattggaa ttagataaat gggcaagttt gtggaattgg tttaacataa
6781 caaattggct gtggtatata aaattattca taatgatagt aggaggcttg gtaggtttaa
6841 gaatagtttt tgctgtactt tctatagtga atagagttag gcagggatat tcaccattat
6901 cgtttcagac ccacctccca accccgaggg gacccgacag gcccgaagga atagaagaag
6961 aaggtggaga gagagacaga gacagatcca ttcgattagt gaacggatct cgacggtatc
7021 gatcacgaga ctagcctcga gcggccgccc ccttcaccga gggcctattt cccatgattc
7081 cttcatattt gcatatacga tacaaggctg ttagagagat aattggaatt aatttgactg
7141 taaacacaaa gatattagta caaaatacgt gacgtagaaa gtaataattt cttgggtagt
7201 ttgcagtttt aaaattatgt tttaaaatgg actatcatat gcttaccgta acttgaaagt
7261 atttcgattt cttggcttta tatatcttgt ggaaaggacg aaacaccggt gtcttcgagg
7321 cttactaaaa gccagataac agtatgcgta tttgcgcgct gatttttgcg gtataagaat
7381 atatactgat atgtataccc gaagtatgtc aaaaagaggt gtgctatgaa gcagcgtatt
7441 acagtgacag ttgacagcga cagctatcag ttgctcaagg catatatgat gtcaatatct
7501 ccggtctggt aagcacaacc atgcagaatg aagcccgtcg tctgcgtgcc gaacgctgga
7561 aagcggaaaa tcaggaaggg atggctgagg tcgcccggtt tattgaaatg aacggctctt
7621 ttgctgacga gaacagggac tggtgaaatg cagtttaagg tttacaccta taaaagagag
7681 agccgttatc gtctgtttgt ggatgtacag agtgatatta ttgacacgcc cgggcgacgg
7741 atggtgatcc ccctggccag tgcacgtctg ctgtcagata aagtctcccg tgaactttac
7801 ccggtggtgc atatcgggga tgaaagctgg cgcatgatga ccaccgatat ggccagtgtg
7861 ccggtctccg ttatcgggga agaagtggct gatctcagcc accgcgaaaa tgacatcaaa
7921 aacgccatta acctgatgtt ctggggaata taaaccctgc aggacgaaga ccagtctttg
7981 tactctcaag agaaatcttg cagaagctac aaagataagg cttcatgccg aaatcaacac
8041 cctgtcattc tatggcaggg tgtttttttg aattctcgac ctcgagacaa atggcagtat
8101 tcatccacaa ttttaaaaga aaagggggga ttggggggta cagtgcaggg gaaagaatag
8161 tagacataat agcaacagac atacaaacta aagaattaca aaaacaaatt acaaaaattc
8221 aaaattttcg ggtttattac agggacagca gagatccact ttggccgcgg ctcgaggggg
//
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