pQL144

LOCUS       pQL144         6446 bp DNA     circular SYN 24-JAN-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 6446)
  AUTHORS   Westlake University
  TITLE     Direct Submission
  JOURNAL   Exported Sep 2, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..6446
                     /mol_type="other DNA"
                     /organism="recombinant plasmid"
     primer_bind     141..160
                     /label=引物 1
     primer_bind     213..262
                     /label=QL386 F
     misc_feature    234..243
                     /label=1
     primer_bind     complement(244..294)
                     /label=QL387  R
     misc_feature    244..253
                     /label=2
     misc_feature    254..263
                     /label=3
     misc_feature    264..273
                     /label=4
     misc_feature    280..427
                     /label=hcmvmin
     misc_feature    349..363
                     /label=New Feature
     primer_bind     complement(412..427)
                     /label=oYW11
     primer_bind     complement(418..439)
                     /label=QL228
     primer_bind     complement(428..449)
                     /label=引物 2
     misc_feature    450..473
                     /label=New Feature
     primer_bind     474..500
                     /label=Oscl13-1
     misc_feature    475..1995
                     /label=SEAP
     primer_bind     complement(1975..2003)
                     /label=Oscl23-2
     primer_bind     complement(1977..1995)
                     /label=Oscl13-2
     terminator      2043..2270
                     /label=bGH PA
     misc_feature    2938..3015
                     /label=SV40 ORI
     CDS             3157..3948
                     /label=Kan/neoR
     primer_bind     complement(4288..4308)
                     /label=M13-rev
     misc_binding    complement(4314..4336)
                     /label=LacO
     promoter        complement(4341..4370)
                     /label=lac
     CDS             complement(5453..6112)
                     /label=AmpR
     promoter        complement(6352..6380)
                     /label=Amp prom
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgtcacttcc
      241 tgtcacttcc tgtcacttcc tgtcacttcc tgtgctagct cgagctcggt acccgggtcg
      301 agtaggcgtg tacggtggga ggcctatata agcagagctc gtttagtgaa ccgtcagatc
      361 gcctggagac gccatccacg ctgttttgac ctccatagaa gacaccggga ccgatccagc
      421 ctccgcggcc ccgaattcga gctcgcccgg ggatcctcta gagtcagctt ctgcatgctg
      481 ctgctgctgc tgctgctggg cctgaggcta cagctctccc tgggcatcat cccagttgag
      541 gaggagaacc cggacttctg gaaccgcgag gcagccgagg ccctgggtgc cgccaagaag
      601 ctgcagcctg cacagacagc cgccaagaac ctcatcatct tcctgggcga tgggatgggg
      661 gtgtctacgg tgacagctgc caggatccta aaagggcaga agaaggacaa actggggcct
      721 gagatacccc tggccatgga ccgcttccca tatgtggctc tgtccaagac atacaatgta
      781 gacaaacatg tgccagacag tggagccaca gccacggcct acctgtgcgg ggtcaagggc
      841 aacttccaga ccattggctt gagtgcagcc gcccgcttta accagtgcaa cacgacacgc
      901 ggcaacgagg tcatctccgt gatgaatcgg gccaagaaag cagggaagtc agtgggagtg
      961 gtaaccacca cacgagtgca gcacgcctcg ccagccggca cctacgccca cacggtgaac
     1021 cgcaactggt actcggacgc cgacgtgcct gcctcggccc gccaggaggg gtgccaggac
     1081 atcgctacgc agctcatctc caacatggac attgacgtga tcctaggtgg aggccgaaag
     1141 tacatgtttc ccatgggaac cccagaccct gagtacccag atgactacag ccaaggtggg
     1201 accaggctgg acgggaagaa tctggtgcag gaatggctgg cgaagcgcca gggtgcccgg
     1261 tatgtgtgga accgcactga gctcatgcag gcttccctgg acccgtctgt gacccatctc
     1321 atgggtctct ttgagcctgg agacatgaaa tacgagatcc accgagactc cacactggac
     1381 ccctccctga tggagatgac agaggctgcc ctgcgcctgc tgagcaggaa cccccgcggc
     1441 ttcttcctct tcgtggaggg tggtcgcatc gaccatggtc atcatgaaag cagggcttac
     1501 cgggcactga ctgagacgat catgttcgac gacgccattg agagggcggg ccagctcacc
     1561 agcgaggagg acacgctgag cctcgtcact gccgaccact cccacgtctt ctccttcgga
     1621 ggctaccccc tgcgagggag ctccatcttc gggctggccc ctggcaaggc ccgggacagg
     1681 aaggcctaca cggtcctcct atacggaaac ggtccaggct atgtgctcaa ggacggcgcc
     1741 cggccggatg ttaccgagag cgagagcggg agccccgagt atcggcagca gtcagcagtg
     1801 cccctggacg aagagaccca cgcaggcgag gacgtggcgg tgttcgcgcg cggcccgcag
     1861 gcgcacctgg ttcacggcgt gcaggagcag accttcatag cgcacgtcat ggccttcgcc
     1921 gcctgcctgg agccctacac cgcctgcgac ctggcgcccc ccgccggcac caccgacgcc
     1981 gcgcacccgg gttaagcggc cgctcgagtc tagagggccc gtttaaaccc gctgatcagc
     2041 ctcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt
     2101 gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca
     2161 ttgtctgagt aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga
     2221 ggattgggaa gacaatagca ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc
     2281 ggaaagaacc agctggggct ctagggggta tccccacgcg ccctgtagcg gcgcattaag
     2341 cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc
     2401 cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc
     2461 tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa
     2521 aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg
     2581 ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac
     2641 actcaaccct atctcggtct attcttttga tttataaggg attttgccga tttcggccta
     2701 ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattaattct gtggaatgtg
     2761 tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
     2821 catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt
     2881 atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc
     2941 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt
     3001 atttatgcag aggccgaggc cgcctctgcc tctgagctat tccagaagta gtgaggaggc
     3061 ttttttggag gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc cattttcgga
     3121 tctgatcaag agacaggatg aggatcgttt cgcatgattg aacaagatgg attgcacgca
     3181 ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca acagacaatc
     3241 ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt tctttttgtc
     3301 aagaccgacc tgtccggtgc cctgaatgaa ctgcaggacg aggcagcgcg gctatcgtgg
     3361 ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg
     3421 gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca ccttgctcct
     3481 gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct tgatccggct
     3541 acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac tcggatggaa
     3601 gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc gccagccgaa
     3661 ctgttcgcca ggctcaaggc gcgcatgccc gacggcgagg atctcgtcgt gacccatggc
     3721 gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt catcgactgt
     3781 ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg tgatattgct
     3841 gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc
     3901 gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc gggactctgg
     3961 ggttcgaaat gaccgaccaa gcgacgccca acctgccatc acgagatttc gattccaccg
     4021 ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc
     4081 tccagcgcgg ggatctcatg ctggagttct tcgcccaccc caacttgttt attgcagctt
     4141 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
     4201 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tgtataccgt
     4261 cgacctctag ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
     4321 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
     4381 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
     4441 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
     4501 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     4561 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     4621 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     4681 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     4741 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     4801 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     4861 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     4921 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     4981 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     5041 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     5101 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
     5161 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     5221 ctggtagcgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     5281 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     5341 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
     5401 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
     5461 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
     5521 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
     5581 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
     5641 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
     5701 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
     5761 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
     5821 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
     5881 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
     5941 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
     6001 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
     6061 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
     6121 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
     6181 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
     6241 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
     6301 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
     6361 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
     6421 ttccccgaaa agtgccacct gacgtc
//