pSL552

LOCUS       pSL552                7471 bp DNA     circular SYN 30-MAR-2026
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7471)
  AUTHORS   Westlake University
  TITLE     Direct Submission
  JOURNAL   Exported Aug 28, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..7471
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     primer_bind     138..159
                     /label=oSCL1174
     polyA_signal    143..264
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     143..172
                     /label=OXB016
     primer_bind     143..168
                     /label=OXB008
     primer_bind     complement(180..199)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(220..240)
                     /label=PSG5-R(SV40-R)
     primer_bind     234..253
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(236..277)
                     /label=OXB007
     primer_bind     253..288
                     /label=OXB006
     primer_bind     270..287
                     /label=Oscl31-4
     primer_bind     270..287
                     /label=oSCL457
     misc_feature    complement(271..1830)
                     /label=SEAP
     primer_bind     271..323
                     /label=oSCL795
     primer_bind     271..287
                     /label=oSCL548
     primer_bind     271..287
                     /label=oSCL776
     primer_bind     271..287
                     /label=oSCL083
     polyA_signal    complement(271..279)
                     /label=SV40 late polyA
     primer_bind     274..289
                     /label=oSCL475
     primer_bind     complement(682..712)
                     /label=Oscl50-1
     primer_bind     682..712
                     /label=Oscl50-2
     primer_bind     complement(765..797)
                     /label=oSCL547
     primer_bind     778..809
                     /label=oSCL546
     primer_bind     complement(1597..1625)
                     /label=oSCL544
     primer_bind     1606..1642
                     /label=oSCL545
     primer_bind     complement(1820..1869)
                     /label=OXB005
     primer_bind     complement(1824..1837)
                     /label=Oscl31-3
     primer_bind     complement(1824..1837)
                     /label=oSCL775
     primer_bind     complement(1824..1836)
                     /label=oSCL456
     misc_feature    complement(1845..1876)
                     /label=Pmin
     primer_bind     1845..1864
                     /label=OXB004
     misc_feature    complement(1904..1998)
                     /label=5*UAS
     primer_bind     complement(1969..1979)
                     /label=OXB003
     primer_bind     complement(1988..2004)
                     /label=OXB003
     primer_bind     complement(1994..2004)
                     /label=oSCL1173
     misc_feature    2003..2590
                     /label=CMV
     primer_bind     2561..2590
                     /label=OX009
     primer_bind     complement(2634..2678)
                     /label=oSCL1135
     primer_bind     2634..2653
                     /label=T7
     primer_bind     complement(2634..2653)
                     /label=oSCL1121
     primer_bind     2655..2688
                     /label=OX009
     primer_bind     2660..2690
                     /label=oSCL291
     CDS             2666..3106
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="DNA binding domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 DNA binding domain
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
     misc_feature    2666..2677
                     /label=SEAP
     primer_bind     3082..3124
                     /label=OXB011 72%GC
     primer_bind     complement(3083..3106)
                     /label=OXB010
     primer_bind     complement(3084..3136)
                     /label=oSCL292
     primer_bind     3107..3136
                     /label=oSCL388
     primer_bind     3107..3126
                     /label=oLZ048 NSP3-F
     primer_bind     3107..3124
                     /label=oLZ147 NS3a F
     primer_bind     3107..3123
                     /label=oYF0033
     primer_bind     complement(3108..3136)
                     /label=oLZ145 DNCR2-GNCR1 R
     primer_bind     3110..3155
                     /label=oYF0025
     primer_bind     complement(3117..3136)
                     /label=oSCL075
     primer_bind     3121..3136
                     /label=oLZ146 GNCR1-N F
     primer_bind     3132..3155
                     /label=oYF009
     CDS             3137..3724
                     /codon_start=1
                     /label=NS3a
                     /translation="KKKGSVVIVGRINLSGDTAYAQQTRGEEGCQETSQTGRDKNQVEG
                     EVQIVSTATQTFLATSINGVLWTVYHGAGTRTIASPKGPVTQMYTNVDKDLVGWQAPQG
                     SRSLTPCTCGSSDLYLVTRHADVIPVRRRGDSRGRLLSPRPISYLKGSAGGPLLCPAGH
                     AVGIFRAAVSTRGVAKAVDFIPVESLETTMRSP"
     primer_bind     3137..3155
                     /label=oFY001
     primer_bind     3137..3155
                     /label=oYF0013
     primer_bind     complement(3705..3730)
                     /label=oLZ148 NS3a R
     primer_bind     complement(3706..3764)
                     /label=Primer 1
     primer_bind     complement(3706..3744)
                     /label=oSCL768
     primer_bind     complement(3706..3733)
                     /label=oYF0014
     primer_bind     complement(3706..3730)
                     /label=oYF0026
     primer_bind     complement(3706..3724)
                     /label=oFY002
     primer_bind     complement(3706..3724)
                     /label=oYF0010
     primer_bind     3710..3729
                     /label=oYF0033
     primer_bind     complement(3711..3769)
                     /label=oSCL766
     primer_bind     complement(3711..3730)
                     /label=oYF0035
     primer_bind     3711..3729
                     /label=oLZ147 NS3a F
     misc_feature    3731..3775
                     /label=P2A
     primer_bind     3731..3771
                     /label=Primer 1
     primer_bind     complement(3731..3767)
                     /label=oSCL475
     primer_bind     complement(3738..3788)
                     /label=oSCL798
     primer_bind     complement(3752..3775)
                     /label=oSCL767
     primer_bind     3772..3815
                     /label=oSCL762
     primer_bind     3772..3790
                     /label=oSCL764
     primer_bind     3790..3824
                     /label=oYF0011
     CDS             3791..4489
                     /codon_start=1
                     /label=GNCR1-N
                     /translation="DIEKLCKKAEEEAKEAQEKADELRQRHPDSQAAEDAEDLANLAVA
                     AVLTACLLAQEHPNADIAKLCIKAASEAAEAASKAAELAQRHPDSQAARDAIKLASQAA
                     RAVILAIMLAAENPNADIAKLCIKAASEAAEAASKAAELAQRHPDSQAARDAIKLASQA
                     AEAVERAIWLAAENPNADIAKKCIKAASEAAEEASKAAEEAQRHPDSQKARDEIKEASQ
                     KAEEVKERCKS"
     primer_bind     3791..3824
                     /label=oYF005
     primer_bind     3791..3818
                     /label=oYF0019
     primer_bind     3791..3815
                     /label=oLZ146 GNCR1-N F
     primer_bind     complement(4466..4504)
                     /label=OXB012
     primer_bind     complement(4468..4509)
                     /label=oYF006
     primer_bind     complement(4468..4509)
                     /label=oYF0020
     primer_bind     complement(4468..4490)
                     /label=oYF0012
     primer_bind     4480..4538
                     /label=OXB013
     primer_bind     complement(4519..4539)
                     /label=oSCL292
     primer_bind     4519..4534
                     /label=oXQ080
     misc_feature    4520..4669
                     /label=VPR
     CDS             4520..4669
                     /label=VP64
     misc_feature    4520..4558
                     /label=VP16 minimal
                     
     primer_bind     4520..4534
                     /label=oSCL265
     misc_feature    4559..4597
                     /label=VP16 minimal
                     
     misc_feature    4598..4636
                     /label=VP16 minimal
                     
     primer_bind     complement(4654..4707)
                     /label=OXB014
     primer_bind     complement(4655..4673)
                     /label=oSCL266
     primer_bind     complement(4655..4670)
                     /label=oXQ082
     polyA_signal    4679..4727
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     4691..4731
                     /label=OXB017
     repeat_region   4734..4874
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   4734..4863
                     /label=AAV2 ITR
     rep_origin      4949..5404
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5036..5055)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5246..5267
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5421..5440)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5540..5562
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5600..5618)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5686..5790
                     /gene="bla"
                     /label=AmpR promoter
     CDS             5791..6651
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6009..6028)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6822..7410
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7311..7330
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggcctcta gcaacttgtt tattgcagct tataatggtt acaaataaag
      181 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
      241 gtccaaactc atcaatgtat cttatcgcga tcatgtctgc tcgaagcggc cggccgcccc
      301 gactctgctg taacccgggt gcgcggcgtc ggtggtgccg gcggggggcg ccaggtcgca
      361 ggcggtgtag ggctccaggc aggcggcgaa ggccatgacg tgcgctatga aggtctgctc
      421 ctgcacgccg tgaaccaggt gcgcctgcgg gccgcgcgcg aacaccgcca cgtcctcgcc
      481 tgcgtgggtc tcttcgtcca ggggcactgc tgactgctgc cgatactcgg ggctcccgct
      541 ctcgctctcg gtaacatccg gccgggcgcc gtccttgagc acatagcctg gaccgtttcc
      601 gtataggagg accgtgtagg ccttcctgtc ccgggccttg ccaggggcca gcccgaagat
      661 ggagctccct cgcagggggt agcctccgaa ggagaacacg tgggagtggt cggcagtgac
      721 gaggctcagc gtgtcctcct cgctggtgag ctggcccgcc ctctcaatgg cgtcgtcgaa
      781 catgatggtc tcagtcagtg cccggtaagc cctgctttca tgatgaccat ggtcgatgcg
      841 accaccctcc acgaagagga agaagccgcg ggggttcctg ctcagcaggc gcagggcagc
      901 ctctgtcatc tccatcaggg aggggtccag tgtggagtct cggtggatct cgtatttcat
      961 gtctccaggc tcaaagagac ccatgagatg ggtcacagac gggtccaggg aagcctgcat
     1021 gagctcagtg cggttccaca cataccgggc accctggcgc ttcgccagcc attcctgcac
     1081 cagattcttc ccgtccagcc tggtcccacc ttggctgtag tcatctgggt actcagggtc
     1141 tggggttccc atgcgaaaca tgtactttcg gcctccacct aggatcacgt caatgtccat
     1201 gttggagatg agctgcgtag cgatgtcctg gcacccctcc tggcgggccg aggcaggcac
     1261 gtcggcgtcc gagtaccagt tgcggttcac cgtgtgggcg taggtgccgg ctggcgaggc
     1321 gtgctgcact cgtgtggtgg ttaccactcc cactgacttc cctgctttct tggcccgatt
     1381 catcacggag atgacctcgt tgccgcgtgt cgtgttgcac tggttaaagc gggcggctgc
     1441 actcaagcca atggtctgga agttgccctt gaccccgcac aggtaggccg tggctgtggc
     1501 tccactgtct ggcacatgtt tgtctacatt gtatgtcttg gacagagcca catatgggaa
     1561 gcggtccatg gccaggggta tctcaggccc cagtttgtcc ttcttctgcc cttttaggat
     1621 tctggcagct gtcaccgtag acacccccat cccatcgccc aggaagatga tgaggttctt
     1681 ggcggctgtc tgtgcaggct gcagcttctt ggcggcaccc agggcctcgg ctgcctcgcg
     1741 gttccagaag tccgggttct cctcctcaac tgggatgatg cccagggaga gctgtagcct
     1801 caggcccagc agcagcagca gcagcagcat ggtggcgttc tagactggaa gtcgagcttc
     1861 cattatatac cctctagtgt ctaagtaggc cgccgagtct agtctcggag gacagtactc
     1921 cgctcggagg acagtactcc gctcggagga cagtactccg ctcggaggac agtactccgc
     1981 tcggaggaca gtactccgac gcgttgacat tgattattga ctagttatta atagtaatca
     2041 attacggggt cattagttca tagcccatat atggagttcc gcgttacata acttacggta
     2101 aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat
     2161 gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg
     2221 taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac
     2281 gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt
     2341 cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat gcggttttgg
     2401 cagtacatca atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc
     2461 attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt
     2521 aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata
     2581 agcagagctc tctggctaac tagagaaccc actgcttact ggcttatcga aattaatacg
     2641 actcactata gggaagctcg ccaccatgaa gctactgtct tctatcgaac aagcatgcga
     2701 tatttgccga cttaaaaagc tcaagtgctc caaagaaaaa ccgaagtgcg ccaagtgtct
     2761 gaagaacaac tgggagtgtc gctactctcc caaaaccaaa aggtctccgc tgactagggc
     2821 acatctgaca gaagtggaat caaggctaga aagactggaa cagctatttc tactgatttt
     2881 tcctcgagaa gaccttgaca tgattttgaa aatggattct ttacaggata taaaagcatt
     2941 gttaacagga ttatttgtac aagataatgt gaataaagat gccgtcacag atagattggc
     3001 ttcagtggag actgatatgc ctctaacatt gagacagcat agaataagtg cgacatcatc
     3061 atcggaagag agtagtaaca aaggtcaaag acagttgact gtatcggcca gcggaagtgg
     3121 cggaggaggc gacgtcaaga agaagggcag cgtggtgatc gtgggcagaa tcaacctgag
     3181 cggcgacacc gcctacgccc agcagaccag aggcgaggag ggctgccagg agaccagcca
     3241 gaccggcaga gacaagaacc aggtggaggg cgaggtgcag atcgtgagca ccgccaccca
     3301 gaccttcctg gccaccagca tcaacggcgt gctgtggacc gtgtaccacg gcgccggcac
     3361 cagaaccatc gccagcccca agggccccgt gacccagatg tacacgaacg tggacaagga
     3421 cttagtgggc tggcaggccc cccaaggcag cagaagcctg accccctgca cctgcggcag
     3481 cagcgacctg tacctggtga ccagacacgc cgacgtgatc cccgtgagaa gaagaggcga
     3541 cagcagaggc cgcctgctga gccccagacc catcagctat ctgaaaggct cagctggcgg
     3601 ccccctgttg tgtcccgccg gccatgccgt gggcatcttc agagccgccg tgagcaccag
     3661 aggcgtggcc aaggccgtgg acttcatccc cgtggagagc ctggagacca ccatgagaag
     3721 ccccgctagc ggagctacta acttcagcct gctgaagcag gctggtgacg tcgaggagaa
     3781 tcctggcccc gacatcgaga agctgtgcaa gaaggcggaa gaggaggcca aggaggccca
     3841 ggagaaggcc gacgagctga gacaacgaca ccccgattcg caggcggcgg aagacgcgga
     3901 ggatctcgca aatctggctg tcgccgccgt gctgaccgct tgtctgctgg cccaggagca
     3961 tcccaacgcc gatatcgcta agctgtgcat caaggccgcg agcgaagctg cggaggccgc
     4021 gtcaaaagcc gccgagctgg cccaacggca cccagacagt caagcagcca gagatgctat
     4081 caaactcgcc tctcaagccg cccgcgccgt gatcctggcc atcatgctcg ccgcagagaa
     4141 tcccaacgct gatattgcaa agctatgtat taaggcggcc agcgaagccg cagaggctgc
     4201 ctcgaaagct gcggaacttg cacagcggca ccccgactcc caggccgccc gagatgcaat
     4261 taaactggcc agtcaggccg ctgaggcggt ggagagagcc atctggctgg ccgccgagaa
     4321 ccccaatgcc gatatcgcca aaaaatgcat caaagcggcc tccgaagcag ccgaggaggc
     4381 ctctaaagct gccgaagagg ctcaaagaca cccagacagc cagaaggcac gcgacgagat
     4441 caaggaggcc tctcagaagg ccgaggaggt gaaggagaga tgcaagagcg gtggaggaag
     4501 cggaggaggt tctggaagcg acgcattgga cgattttgat ctggatatgc tgggaagtga
     4561 cgccctcgat gattttgacc ttgacatgct tggttcggat gcccttgatg actttgacct
     4621 cgacatgctc ggcagtgacg cccttgatga tttcgacctg gacatgctgt gaggatcccg
     4681 gcaataaaaa gacagaataa aacgcacggg tgttgggtcg tttgttcggc cgcaggaacc
     4741 cctagtgatg gagttggcca ctccctctct gcgcgctcgc tcgctcactg aggccgggcg
     4801 accaaaggtc gcccgacgcc cgggctttgc ccgggcggcc tcagtgagcg agcgagcgcg
     4861 cagctgcctg caggggcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc
     4921 acaccgcata cgtcaaagca accatagtac gcgccctgta gcggcgcatt aagcgcggcg
     4981 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccttagc gcccgctcct
     5041 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
     5101 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt
     5161 gatttgggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg
     5221 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac
     5281 tctatctcgg gctattcttt tgatttataa gggattttgc cgatttcggt ctattggtta
     5341 aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgtttaca
     5401 attttatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagccccga
     5461 cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac
     5521 agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg
     5581 aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata
     5641 ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt
     5701 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
     5761 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
     5821 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
     5881 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
     5941 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt
     6001 aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt
     6061 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat
     6121 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac
     6181 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg
     6241 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc
     6301 ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa
     6361 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag
     6421 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct
     6481 gataaatctg gagccggtga gcgtggaagc cgcggtatca ttgcagcact ggggccagat
     6541 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa
     6601 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac
     6661 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
     6721 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
     6781 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     6841 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     6901 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     6961 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     7021 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     7081 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     7141 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     7201 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     7261 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     7321 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     7381 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     7441 ctggcctttt gctggccttt tgctcacatg t
//