pAAVLINK-EF1alpha-HA-ASH1L-A

LOCUS      pAAVLINK-EF1alpha-HA-ASH1L-A              6639 bp DNA     circular SYN 10-AUG-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6639)
  AUTHORS   Stavrini Papadaki
  TITLE     Direct Submission
  JOURNAL   Exported Jun 16, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..6639
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
                     /note="color: #ffe4c4"
     promoter        158..700
                     /label=EF1-alpha
                     /note="Chimeric promoter of ef1a and htlv 5' UTR"
     promoter        187..398
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             411..679
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     misc_feature    505..510
                     /label=SDS
     CDS             716..742
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             749..3779
                     /codon_start=1
                     /gene="ASH1L"
                     /gene_synonym="ASH1; ASH1L1; KMT2H; MRD52"
                     /product="histone-lysine N-methyltransferase ASH1L 
                     isoform2"
                     /EC_number="2.1.1.359; 2.1.1.367"
                     /label=ASH1L
                     /note="isoform 2 is encoded by transcript variant 
                     2;probable histone-lysine N-methyltransferase ASH1L; 
                     ASH1-like protein; lysine N-methyltransferase 2H; absent 
                     small and homeotic disks protein 1 homolog; ash1 (absent, 
                     small, or homeotic)-like"
                     /db_xref="GI:110349788"
                     /db_xref="CCDS:CCDS1113.2"
                     /db_xref="GeneID:55870"
                     /db_xref="HGNC:HGNC:19088"
                     /db_xref="MIM:607999"
                     /protein_id="NP_060959.2"
                     /translation="DPRNTAMLGLGSDSEGFSRKSPSAISTGTLVSKREVELEKNTKEE
                     EDLRKRNRERNIEAGKDDGLTDAQQQFSVKETNFSEGNLKLKIGLQAKRTKKPPKNLEN
                     YVCRPAIKTTIKHPRKALKSGKMTDEKNEHCPSKRDPSKLYKKADDVAAIECQSEEVIR
                     LHSQGENNPLSKKLSPVHSEMADYINATPSTLLGSRDPDLKDRALLNGGTSVTEKLAQL
                     IATCPPSKSSKTKPKKLGTGTTAGLVSKDLIRKAGVGSVAGIIHKDLIKKPTISTAVGL
                     VTKDPGKKPVFNAAVGLVNKDSVKKLGTGTTAVFINKNLGKKPGTITTVGLLSKDSGKK
                     LGIGIVPGLVHKESGKKLGLGTVVGLVNKDLGKKLGSTVGLVAKDCAKKIVASSAMGLV
                     NKDIGKKLMSCPLAGLISKDAINLKAEALLPTQEPLKASCSTNINNQESQELSESLKDS
                     ATSKTFEKNVVRQNKESILEKFSVRKEIINLEKEMFNEGTCIQQDSFSSSEKGSYETSK
                     HEKQPPVYCTSPDFKMGGASDVSTAKSPFSAVGESNLPSPSPTVSVNPLTRSPPETSSQ
                     LAPNPLLLSSTTELIEEISESVGKNQFTSESTHLNVGHRSVGHSISIECKGIDKEVNDS
                     KTTHIDIPRISSSLGKKPSLTSESSIHTITPSVVNFTSLFSNKPFLKLGAVSASDKHCQ
                     VAESLSTSLQSKPLKKRKGRKPRWTKVVARSTCRSPKGLELERSELFKNVSCSSLSNSN
                     SEPAKFMKNIGPPSFVDHDFLKRRLPKLSKSTAPSLALLADSEKPSHKSFATHKLSSSM
                     CVSSDLLSDIYKPKRGRPKSKEMPQLEGPPKRTLKIPASKVFSLQSKEEQEPPILQPEI
                     EIPSFKQGLSVSPFPKKRGRPKRQMRSPVKMKPPVLSVAPFVATESPSKLESESDNHRS
                     SSDFFESEDQLQDPDDLDDSHRPSVCSMSDLEMEPDKKITKRNNGQLMKTIIRKINKMK
                     TLKRKKLLNQILSSSVESSNK"
     misc_feature    876..881
                     /label=SDS
     misc_feature    1427..1432
                     /label=SDS
     misc_feature    2312..2317
                     /label=SDS
     intron          3780..3820
                     /label=Splicing Donor
     misc_feature    3824..3857
                     /label=loxP-JT1571
     repeat_region   3902..4042
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     misc_feature    3902..4031
                     /label=AAV2 ITR
     repeat_region   3903..4043
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     rep_origin      4117..4572
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        4854..4958
                     /gene="bla"
                     /label=AmpR promoter
     CDS             4959..5819
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      5990..6578
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtgtcgac tctagaggat ctgcgatcgc tccggtgccc
      181 gtcagtgggc agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca
      241 attgaacggg tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact
      301 ggctccgcct ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga
      361 acgttctttt tcgcaacggg tttgccgcca gaacacagct gaagcttcga ggggctcgca
      421 tctctccttc acgcgcccgc cgccctacct gaggccgcca tccacgccgg ttgagtcgcg
      481 ttctgccgcc tcccgcctgt ggtgcctcct gaactgcgtc cgccgtctag gtaagtttaa
      541 agctcaggtc gagaccgggc ctttgtccgg cgctcccttg gagcctacct agactcagcc
      601 ggctctccac gctttgcctg accctgcttg ctcaactcta cgtctttgtt tcgttttctg
      661 ttctgcgccg ttacagatcc aagctgtgac cggcgcctac cgtacggcca ccatgtaccc
      721 ttatgacgta cctgactatg ctggatctga ccctagaaat actgctatgt taggattggg
      781 ttctgattcc gaaggttttt caagaaagag tccttctgcc atcagtactg gcacattggt
      841 cagtaagaga gaagtagagc tagaaaaaaa cacaaaggag gaagaggacc ttcgcaaacg
      901 gaatcgagaa agaaacatcg aagctgggaa agatgatggt ttgactgatg cacagcaaca
      961 gttttcagtg aaagaaacaa acttttcaga gggaaattta aaattgaaaa ttggcctcca
     1021 ggctaagaga actaaaaaac ctccaaagaa cttggagaac tatgtatgtc gacctgccat
     1081 aaaaacaact attaagcacc caaggaaagc acttaaaagt ggaaagatga cggatgaaaa
     1141 gaatgaacac tgtccttcaa aacgagaccc ttcaaagttg tacaagaaag cagatgatgt
     1201 tgcagccatt gaatgccagt ctgaagaagt catccgtctt cattcacagg gagaaaacaa
     1261 tcctttgtct aagaagctgt ctccagtaca ctcagaaatg gcagattata ttaatgcaac
     1321 gccatctact cttcttggta gccgggatcc tgatttaaag gacagagcat tacttaatgg
     1381 aggaactagt gtaacagaaa agttggcaca gctgattgct acctgtcctc cttccaagtc
     1441 ttccaagaca aaaccgaaga agttaggaac tggcactaca gcaggattgg ttagcaagga
     1501 tttgatcagg aaagcaggtg ttggctctgt agctggaata atacataagg acttaataaa
     1561 aaagccaacc atcagcacag cagttggatt ggtaactaaa gatcctggga aaaagccagt
     1621 gtttaatgca gcagtaggat tggtcaataa ggactctgtg aaaaaactgg gaactggcac
     1681 tacagcggta ttcattaata aaaacttagg caaaaagcca ggaactatca ctacagtagg
     1741 actgctaagc aaagattcag gaaagaagct aggaattggt attgttccag gtttagtgca
     1801 taaagagtct ggcaagaagt taggacttgg cactgtggtt ggactggtta ataaagattt
     1861 gggaaagaaa ttgggttcta ctgttggcct agtggccaag gactgtgcaa agaagattgt
     1921 agcaagttca gcaatgggat tggttaataa ggacattgga aagaaactaa tgagttgtcc
     1981 tttggcaggt ctgatcagta aagatgccat aaaccttaaa gccgaagcac tgctccccac
     2041 tcaggaaccg cttaaggctt cttgtagtac aaacatcaat aatcaggaaa gtcaggaact
     2101 ttctgaatcc ctgaaagata gtgccaccag caaaactttt gaaaagaatg ttgtacggca
     2161 gaataaagaa agcatattgg aaaagttctc agtacgaaaa gaaatcatta atttggagaa
     2221 agaaatgttt aatgaaggaa catgcattca gcaagacagt ttctcatcca gtgaaaaggg
     2281 atcttatgaa acctcaaagc atgaaaagca gcctcctgta tattgcactt ctccggactt
     2341 taaaatggga ggtgcttctg atgtatctac cgctaaatcc ccattcagtg cagtaggaga
     2401 aagcaatctc ccttccccat cacctactgt atctgttaat cctttaacca gaagtccccc
     2461 tgaaacttct tcacagttgg ctcctaatcc attactttta agttctacta cagaactaat
     2521 cgaagaaatt tctgaatctg ttggaaagaa ccagtttact tctgaaagta cccacttgaa
     2581 cgttggtcat aggtcagttg gtcatagtat aagtattgaa tgtaaaggga ttgataaaga
     2641 ggtaaatgat tcaaaaacta cccatataga tattccaaga ataagctctt cccttggaaa
     2701 aaagccaagt ttgacttctg aatccagcat tcatactatt actccttcag ttgttaactt
     2761 cactagttta tttagtaata agcctttttt aaaactgggt gcagtatctg catcagacaa
     2821 acactgccaa gttgctgaaa gcctaagtac tagtttgcag tccaaaccat taaaaaaaag
     2881 aaaaggaaga aaacctcggt ggactaaagt ggtggcaaga agcacatgcc ggtctccaaa
     2941 agggctagaa ttagaaagat cagagctttt taaaaacgtt tcatgtagct cactatcaaa
     3001 tagtaattct gagccagcca agtttatgaa aaacattgga cccccttcat ttgtagatca
     3061 tgacttcctt aaacgccgat tgccaaagtt gagcaaatcc acagctccat ctcttgctct
     3121 cttagctgat agtgaaaaac catctcataa gtcttttgct actcacaaac tatcctccag
     3181 tatgtgtgtc tctagtgacc ttttgtctga tatttataag cccaaaagag gaaggcctaa
     3241 atctaaggag atgcctcaac tggaagggcc acctaaaagg actttaaaaa tccctgcttc
     3301 taaagtgttt tctttacagt ctaaggaaga acaagaaccc ccaattttac agccagaaat
     3361 tgaaatccct tccttcaaac aaggtctgtc tgtgtctcct tttccaaaaa agagaggcag
     3421 gcctaagagg caaatgaggt caccagtcaa gatgaagcca cctgtactgt cagtggctcc
     3481 atttgttgcc actgaaagtc caagcaagct agaatctgaa agtgacaacc atagaagtag
     3541 cagtgatttc tttgagagcg aggatcaact tcaggatcca gatgacctag atgacagtca
     3601 taggccaagt gtctgtagta tgagtgacct tgagatggaa ccagataaaa aaattaccaa
     3661 gagaaacaat ggacaattaa tgaaaacaat tatccgcaaa ataaataaaa tgaagacttt
     3721 aaagagaaag aaactgttga atcagattct ttcaagttct gtagaatcaa gtaataaagg
     3781 taagtatcaa ggttacaaga caggtttaag gagaccaata agctattatt cgtatagcat
     3841 acattatacg aagttattaa gttatggaga ccggtgtggc acgtgcggac cgagcggccg
     3901 caggaacccc tagtgatgga gttggccact ccctctctgc gcgctcgctc gctcactgag
     3961 gccgggcgac caaaggtcgc ccgacgcccg ggctttgccc gggcggcctc agtgagcgag
     4021 cgagcgcgca gctgcctgca ggggcgcctg atgcggtatt ttctccttac gcatctgtgc
     4081 ggtatttcac accgcatacg tcaaagcaac catagtacgc gccctgtagc ggcgcattaa
     4141 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccttagcgc
     4201 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag
     4261 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca
     4321 aaaaacttga tttgggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc
     4381 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa
     4441 cactcaactc tatctcgggc tattcttttg atttataagg gattttgccg atttcggtct
     4501 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa
     4561 cgtttacaat tttatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc
     4621 agccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat
     4681 ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt
     4741 catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg cctattttta taggttaatg
     4801 tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa
     4861 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     4921 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
     4981 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
     5041 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
     5101 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
     5161 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
     5221 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
     5281 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
     5341 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
     5401 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     5461 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
     5521 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     5581 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     5641 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
     5701 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     5761 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     5821 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     5881 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     5941 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     6001 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     6061 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     6121 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     6181 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     6241 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     6301 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     6361 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     6421 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     6481 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     6541 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     6601 tacggttcct ggccttttgc tggccttttg ctcacatgt
//