pAAVLINKv2-SCP1-Cre-UDeg3a-3'-CUL7-FLAG

价格：元

规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg

相关技术服务：质粒构建    基因合成    质粒大提

联系方式：I47-825O-882O

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pAAVLINKv2-SCP1-Cre-UDeg3a-3'-CUL7-FLAG载体质粒基本信息

质粒名称: pAAVLINKv2-SCP1-Cre-UDeg3a-3'-CUL7-FLAG
出品公司: Wekwikgene
目录编号: 0001858
产品用途: 3' AAVLINK plasmid for CUL7 expression
存储实验室: Zhonghua Lu Lab

载体骨架基本信息
载体名称: pAAV-MCS2
表达系统: 腺病毒
启动子: SCP1
拷贝数: 高拷贝
载体抗性: 氨苄霉素
生长温度: 37 ℃
宿主菌: DH5alpha

插入基因信息
插入基因名称:
CUL7
物种来源: H.sapiens
密码子优化: 未优化

文献引用方法
参考文献部分:
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review

pAAVLINKv2-SCP1-Cre-UDeg3a-3'-CUL7-FLAG质粒图谱载体图谱和pAAVLINKv2-SCP1-Cre-UDeg3a-3'-CUL7-FLAG载体序列质粒序列多克隆位点信息

pAAVLINKv2-SCP1-Cre-UDeg3a-3-CUL7-FLAG载体图谱

pAAVLINKv2-SCP1-Cre-UDeg3a-3'-CUL7-FLAG质粒载体简介

pAAVLINKv2-SCP1-Cre-UDeg3a-3'-CUL7-FLAG质粒序列载体序列

LOCUS       pAAVLINKv2-SCP1-Cre-UDeg3a-3'-CUL7-FLAG            6477 bp DNA     circular SYN 24-NOV-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6477)
  AUTHORS   Stavrini Papadaki
  TITLE     Direct Submission
  JOURNAL   Exported Jun 11, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..6477
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
                     /note="color: #ffe4c4"
     misc_feature    145..225
                     /label=SCP1
     CDS             237..1265
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
                     LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
                     PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
                     LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
                     RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
                     RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
                     DGD"
     CDS             1281..1370
                     /codon_start=1
                     /label=UDeg3a
                     /translation="LLLKLLLLGGGSGPRPRGTRGKGRRIHYKG"
     misc_feature    1295..1300
                     /label=SDS
     misc_feature    1298..1303
                     /label=SDS
     intron          1374..1414
                     /label=Splicing Donor
     protein_bind    1421..1454
                     /label=lox2272-JTZ17
                     /bound_moiety="Cre recombinase"
                     /note="Right element (RE) mutant of loxP (Araki et al., 
                     2010). Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     intron          1455..1546
                     /label=Splicing Acceptor
                     /note="Splicing acceptor"
     CDS             1547..3178
                     /codon_start=1
                     /gene="CUL7"
                     /gene_synonym="3M1; CUL-7; dJ20C7.5; KIAA0076"
                     /product="cullin-7 isoform 2"
                     /label=CUL7
                     /note="isoform 2 is encoded by transcript variant 2"
                     /db_xref="GI:41872646"
                     /db_xref="CCDS:CCDS4881.1"
                     /db_xref="GeneID:9820"
                     /db_xref="HGNC:HGNC:21024"
                     /db_xref="MIM:609577"
                     /protein_id="NP_055595.2"
                     /translation="VNNFLTSSWRDDDFVPRYCEHFNILQNSSSELFGPRAAFLLALQN
                     GCAGALLKLPFLKAAHVSEQFARHIDQQIQGSRIGGAQEMERLAQLQQCLQAVLIFSGL
                     EIATTFEHYYQHYMADRLLGVVSSWLEGAVLEQIGPCFPNRLPQQMLQSLSTSKELQRQ
                     FHVYQLQQLDQELLKLEDTEKKIQVGLGASGKEHKSEKEEEAGAAAVVDVAEGEEEEEE
                     NEDLYYEGAMPEVSVLVLSRHSWPVASICHTLNPRTCLPSYLRGTLNRYSNFYNKSQSH
                     PALERGSQRRLQWTWLGWAELQFGNQTLHVSTVQMWLLLYLNDLKAVSVESLPAFSGLS
                     ADMLNQAIGPLTSSRGPLDLHEQKDIPGGVLKIRDGSKEPRSRWDIVRLIPPQTYLQAE
                     GEDGQNLEKRRNLLNCLIVRILKAHGDEGLHIDQLVCLVLEAWQKGPCPPRGLVSSLGK
                     GSACSSTDVLSCILHLLGKGTLRRHDDRPQVLSYAVPVTVMEPHTESLNPGSSGPNPPL
                     TFHTLQIRSRGVPYASCTATQSFSTFR"
     misc_feature    2196..2201
                     /label=SDS
     misc_feature    2205..2210
                     /label=SDS
     misc_feature    2328..2333
                     /label=SDS
     misc_feature    2647..2652
                     /label=SDS
     misc_feature    2965..2970
                     /label=SDS
     misc_feature    3141..3146
                     /label=SDS
     CDS             3185..3250
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     terminator      3266..3697
                     /label=W3SL
                     /note="Short WPRE and SV40 polyA"
     misc_feature    3410..3415
                     /label=SDS
     repeat_region   3740..3880
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     misc_feature    3740..3869
                     /label=AAV2 ITR
     repeat_region   3741..3881
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     rep_origin      3955..4410
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        4692..4796
                     /gene="bla"
                     /label=AmpR promoter
     CDS             4797..5657
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      5828..6416
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtgtactt atataagggg gtgggggcgc gttcgtcctc
      181 agtcgcgatc gaacactcga gccgagcaga cgtgcctacg gaccgcgtac gccaccatgt
      241 ccaatttact gaccgtacac caaaatttgc ctgcattacc ggtcgatgca acgagtgatg
      301 aggttcgcaa gaacctgatg gacatgttca gggatcgcca ggcgttttct gagcatacct
      361 ggaaaatgct tctgtccgtt tgccggtcgt gggcggcatg gtgcaagttg aataaccgga
      421 aatggtttcc cgcagaacct gaagatgttc gcgattatct tctatatctt caggcgcgcg
      481 gtctggcagt aaaaaccatc cagcaacatt tgggccagct aaacatgctt catcgtcggt
      541 ccgggctgcc acgaccaagt gacagcaatg ctgtttcact ggttatgcgg cggatccgaa
      601 aagaaaacgt tgatgccggt gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt
      661 tcgaccaggt tcgttcactc atggaaaata gcgatcgctg ccaggatata cgtaatctgg
      721 catttctggg gattgcttat aacaccctgt tacgtatagc cgaaattgcc aggatcaggg
      781 ttaaagatat ctcacgtact gacggtggga gaatgttaat ccatattggc agaacgaaaa
      841 cgctggttag caccgcaggt gtagagaagg cacttagcct gggggtaact aaactggtcg
      901 agcgatggat ttccgtctct ggtgtagctg atgatccgaa taactacctg ttttgccggg
      961 tcagaaaaaa tggtgttgcc gcgccatctg ccaccagcca gctatcaact cgcgccctgg
     1021 aagggatttt tgaagcaact catcgattga tttacggcgc taaggatgac tctggtcaga
     1081 gatacctggc ctggtctgga cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg
     1141 ctggagtttc aataccggag atcatgcaag ctggtggctg gaccaatgta aatattgtaa
     1201 tgaactatat ccgtaacctg gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg
     1261 gcgatggagg tggatctggt ctgctgctga aactcctcct cctcggtggt ggttctggtc
     1321 ctcgtccccg tggtactcgt ggtaaaggtc gccgtattca ctacaaagga taagtaagta
     1381 tcaaggttac aagacaggtt taaggagacc aataactagt ataacttcgt ataggatact
     1441 ttatagcaat ttatgaaact gggcttgtcg agacagagaa gactcttgcg tttctgatag
     1501 gcacctattg gtcttactga catccacttt gcctttctct ccacaggtga acaattttct
     1561 gacctcatcc tggcgggatg atgactttgt gccacgctac tgtgagcact ttaatattct
     1621 gcagaactca agctctgaac tgtttgggcc tcgggcagcc ttcttgctgg cgctgcaaaa
     1681 tggctgtgcg ggagccttgc tgaagctccc ttttctcaaa gctgcccacg tgagtgagca
     1741 gttcgcccgg cacattgacc agcagatcca gggcagccgg atcggtggag cccaggaaat
     1801 ggagaggctg gcacagctgc agcaatgcct gcaagctgtc ctgattttct ccggcttgga
     1861 gatagccacc acttttgagc attattacca gcactacatg gcggaccgtc tcctgggcgt
     1921 ggtctcgagc tggctggagg gggccgtgct ggagcagatc ggtccctgct tccccaaccg
     1981 cctcccccag cagatgttgc agagcctgag cacctctaag gagctgcagc gccagttcca
     2041 cgtctaccag ctccagcagc tggatcagga actcctgaag ctggaggata cagagaagaa
     2101 aatacaggtg ggccttgggg ccagtggcaa ggagcacaag agcgagaagg aagaggaagc
     2161 tggggcagca gcagtggtgg atgtggcgga gggagaggag gaagaggagg agaatgagga
     2221 cctctactat gaaggggcaa tgccagaagt gtctgtgctt gtcctgtccc gacactcctg
     2281 gcctgttgcc tcaatctgcc acacactgaa ccccagaacc tgcctgccct cctacctgag
     2341 gggcactttg aacagatact ccaacttcta caacaagagt cagagccacc ctgcccttga
     2401 gcgaggctca cagaggcgac tgcagtggac gtggctgggc tgggctgagc tgcagtttgg
     2461 gaaccagacc ctgcatgtgt ccaccgtgca gatgtggcta ctgctgtatc tcaacgacct
     2521 gaaggcggtc tctgtggaga gtctgccggc gttctcaggg ctctccgcag acatgctcaa
     2581 tcaggcgatt gggcccctca cctcttcaag aggccccctg gaccttcacg agcaaaagga
     2641 tataccagga ggggtcctca agattcgaga tggcagcaag gaacccaggt cgagatggga
     2701 cattgtgcgg ctcatcccac ctcagacgta cctgcaagct gagggtgaag acggccagaa
     2761 cttggagaag agacggaatc ttctgaactg cctcatcgtc cgaatcctca aggcccatgg
     2821 agatgagggg ctgcacattg accagcttgt ctgtctggtg ctggaggctt ggcagaaggg
     2881 cccgtgtcct cccaggggtt tggtcagcag ccttggtaag gggtctgcat gcagcagcac
     2941 tgacgtcctc tcctgcatcc tacacctcct gggcaagggc acgctgagac gccatgacga
     3001 ccggccccag gtgctgtcct atgcagtccc tgtgactgtc atggagcctc acactgagtc
     3061 cctgaaccca ggctcctcag gccccaaccc acccctcacc ttccataccc tacagattcg
     3121 ctcccggggt gtgccctatg cctcctgcac tgccacccag agcttctcta ccttccggtg
     3181 tacagactac aaagaccatg acggtgatta taaagatcat gacatcgact acaaggatga
     3241 cgatgacaag taagaattct gatgactcga gataatcaac ctctggatta caaaatttgt
     3301 gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct
     3361 ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat
     3421 aaatcctggt tagttcttgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg
     3481 acaggggctc ggctgttggg cactgacaat tccgtggtgt ttatttgtga aatttgtgat
     3541 gctattgctt tatttgtaac catctagctt tatttgtgaa atttgtgatg ctattgcttt
     3601 atttgtaacc attataagct gcaataaaca agttaacaac aacaattgca ttcattttat
     3661 gtttcaggtt cagggggaga tgtgggaggt tttttaaaga tctggagacc ggtgtggcac
     3721 gtgcggaccg agcggccgca ggaaccccta gtgatggagt tggccactcc ctctctgcgc
     3781 gctcgctcgc tcactgaggc cgggcgacca aaggtcgccc gacgcccggg ctttgcccgg
     3841 gcggcctcag tgagcgagcg agcgcgcagc tgcctgcagg ggcgcctgat gcggtatttt
     3901 ctccttacgc atctgtgcgg tatttcacac cgcatacgtc aaagcaacca tagtacgcgc
     3961 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac
     4021 ttgccagcgc cttagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg
     4081 ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt
     4141 tacggcacct cgaccccaaa aaacttgatt tgggtgatgg ttcacgtagt gggccatcgc
     4201 cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct
     4261 tgttccaaac tggaacaaca ctcaactcta tctcgggcta ttcttttgat ttataaggga
     4321 ttttgccgat ttcggtctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga
     4381 attttaacaa aatattaacg tttacaattt tatggtgcac tctcagtaca atctgctctg
     4441 atgccgcata gttaagccag ccccgacacc cgccaacacc cgctgacgcg ccctgacggg
     4501 cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt
     4561 gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc
     4621 tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc
     4681 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
     4741 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga
     4801 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
     4861 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag
     4921 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag
     4981 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta
     5041 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg
     5101 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca
     5161 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag
     5221 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc
     5281 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg
     5341 tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc
     5401 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg
     5461 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg
     5521 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga
     5581 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac
     5641 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa
     5701 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
     5761 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     5821 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     5881 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     5941 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
     6001 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     6061 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     6121 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     6181 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     6241 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     6301 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     6361 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     6421 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgt
//

pAAVLINKv2-SCP1-Cre-UDeg3a-3'-CUL7-FLAG载体图谱质粒图谱pdf版和pAAVLINKv2-SCP1-Cre-UDeg3a-3'-CUL7-FLAG载体序列质粒序列等相关资料下载

pAAVLINKv2-SCP1-Cre-UDeg3a-3'-CUL7-FLAG质粒载体应用举例

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